Abstract: Provided is a method for efficiently culturing pluripotent stem cells with higher safety. The present invention relates to a method for culturing pluripotent stem cells, the method comprising culturing an isolated pluripotent stem cells in a pseudo-microgravity environment to proliferate the pluripotent stem cells while maintaining the pluripotent stem cells in an undifferentiated state, thereby forming and growing spheroids of the pluripotent stem cells; and a method for inducing differentiation of pluripotent stem cells by using the method.

Abstract: Disclosed is a rotating culture vessel based on a rotating culture technology using an RWV, by which cell seeding, liquid medium exchange, quality control and so on can be automated and degassing can be conducted simultaneously with liquid medium exchange without disturbing the cells under culture. Also disclosed is an automatic cell culture apparatus using the same. A rotating culture vessel, which contains cells and a liquid culture medium, to be attached to a horizontal rotating shaft of a rotating culture device to three-dimensionally culture the cells, wherein one or more inlets/outlets for supplying cells and a liquid culture medium at the early stage and then taking out the cultured cells, are formed at appropriate position of a flat cylindrical culture container; at least one pair of a supply port and a discharge port for liquid medium exchange is provided on the outer circumferential cylindrical face of the culture container.

Abstract: The mononuclear cell separation apparatus of the present invention has an injecting means (210) for injecting a centrifugation medium from the bottom surface of a container (100) storing a blood sample; a centrifugation means (300) for centrifuging a container (100) containing a centrifugation medium and a blood sample layered in this order from the bottom surface side; a detecting means (400) for detecting a clot present at a mononuclear cell layer after centrifugation; a removing means (220) for removing a detected clot; and a harvesting means (230) for harvesting the mononuclear cell. The mononuclear cell separation method of the present invention includes an injecting step, a centrifuging step, a detection step, a removing step, and a harvesting step corresponding to each constituent element of the mononuclear cell separation apparatus of the present invention.

Abstract: Disclosed is a rotating culture vessel based on a rotating culture technology using an RWV, by which cell seeding, liquid medium exchange, quality control and so on can be automated and degassing can be conducted simultaneously with liquid medium exchange without disturbing the cells under culture. Also disclosed is an automatic cell culture apparatus using the same. A rotating culture vessel, which contains cells and a liquid culture medium, to be attached to a horizontal rotating shaft of a rotating culture device to three-dimensionally culture the cells, wherein one or more inlets/outlets for supplying cells and a liquid culture medium at the early stage and then taking out the cultured cells, are formed at appropriate position of a flat cylindrical culture container; at least one pair of a supply port and a discharge port for liquid medium exchange is provided on the outer circumferential cylindrical face of the culture container.

Abstract: Disclosed is a rotating culture vessel based on a rotating culture technology using an RWV, by which cell seeding, liquid medium exchange, quality control and so on can be automated and degassing can be conducted simultaneously with liquid medium exchange without disturbing the cells under culture. Also disclosed is an automatic cell culture apparatus using the same. A rotating culture vessel, which contains cells and a liquid culture medium, to be attached to a horizontal rotating shaft of a rotating culture device to three-dimensionally culture the cells, wherein one or more inlets/outlets for supplying cells and a liquid culture medium at the early stage and then taking out the cultured cells, are formed at appropriate position of a flat cylindrical culture container; at least one pair of a supply port and a discharge port for liquid medium exchange is provided on the outer circumferential cylindrical face of the culture container.

Abstract: This large-scale cell culture system is for performing large-scale culture of cells by performing, in a closed system, subculture and transfer of spheroids and a culture medium by use of a vessel having a syringe structure, wherein the vessel comprises a front flange and a back flange which have a same circular outer shape and which are provided integrally with both ends of an outer cylinder part of the vessel, and the vessel allows rotary culture, utilizing the front flange and the back flange in a state where a head of the vessel is closed by a detachable cap and a space, in the vessel, closed by a gasket of a plunger is filled with a cell liquid obtained by suspending cells in a culture medium.

Abstract: The rotating culture device includes a tubular culture vessel long in an axial direction, a support mechanism which supports the culture vessel so that the culture vessel is rotatable about an axis thereof, rotation drive section which rotationally drives the culture vessel, tilting section which tilts the support mechanism so that the axis of the culture vessel is tilted in a vertical direction, and control section which controls operation of the rotation drive section and operation of the tilting section.

Abstract: Provided is a method for efficiently culturing pluripotent stem cells with higher safety. The present invention relates to a method for culturing pluripotent stem cells, the method comprising culturing an isolated pluripotent stem cells in a pseudo-microgravity environment to proliferate the pluripotent stem cells while maintaining the pluripotent stem cells in an undifferentiated state, thereby forming and growing spheroids of the pluripotent stem cells; and a method for inducing differentiation of pluripotent stem cells by using the method.

Abstract: The device is configured from: a reflective surface shape controllable mirror in which a band-shaped X-ray reflective surface 2 is formed on a central portion of a front surface of a substrate 1, reference planes 3 are formed along both sides of the X-ray reflective surface, and a plurality of piezoelectric elements 4 are attached to at least one of front and back surfaces of the substrate so as to be arranged in the longitudinal direction of the X-ray reflective surface on both side portions of the substrate, and a multichannel control system for applying a voltage to each of the piezoelectric elements.

Abstract: Provided is a small-scale cell culture apparatus having a culture medium replacement function for culturing cells in a small number of Petri dishes, the cell culture apparatus being capable of performing automatic replacement of the culture medium and cell culture within the same cavity and significantly reducing the apparatus cost. At least one set of upper and lower discs is arranged inside an incubator chamber 1. Petri dishes 4, 6 in each of which cells and a culture medium are put are held on lower discs 18, 19, and top lids 5, 7 are held on upper discs 19, 30. The lower side of each of the top lids is open.

Abstract: Provided is a small-scale cell culture apparatus having a culture medium replacement function for culturing cells in a small number of Petri dishes, the cell culture apparatus being capable of performing automatic replacement of the culture medium and cell culture within the same cavity and significantly reducing the apparatus cost. At least one set of upper and lower discs is arranged inside an incubator chamber 1. Petri dishes 4, 6 in each of which cells and a culture medium are put are held on lower discs 18, 19, and top lids 5, 7 are held on upper discs 19, 30. The lower side of each of the top lids is open.

Abstract: The device is configured from: a reflective surface shape controllable mirror in which a band-shaped X-ray reflective surface 2 is formed on a central portion of a front surface of a substrate 1, reference planes 3 are formed along both sides of the X-ray reflective surface, and a plurality of piezoelectric elements 4 are attached to at least one of front and back surfaces of the substrate so as to be arranged in the longitudinal direction of the X-ray reflective surface on both side portions of the substrate, and a multichannel control system for applying a voltage to each of the piezoelectric elements.

Abstract: Disclosed is a rotating culture vessel based on a rotating culture technology using an RWV, by which cell seeding, liquid medium exchange, quality control and so on can be automated and degassing can be conducted simultaneously with liquid medium exchange without disturbing the cells under culture. Also disclosed is an automatic cell culture apparatus using the same. A rotating culture vessel, which contains cells and a liquid culture medium, to be attached to a horizontal rotating shaft of a rotating culture device to three-dimensionally culture the cells, wherein one or more inlets/outlets for supplying cells and a liquid culture medium at the early stage and then taking out the cultured cells, are formed at appropriate position of a flat cylindrical culture container; at least one pair of a supply port and a discharge port for liquid medium exchange is provided on the outer circumferential cylindrical face of the culture container.

Abstract: A pressure switch includes an external force transmission mechanism for separating a cover from a housing. The cover and the external force transmission mechanism defines a first pressure chamber, and the housing and the external force transmission mechanism defines a second pressure chamber. In the second pressure chamber, a movable piece having first and second movable members arranged opposite to each other and one or more fixed contacts are disposed. The first and second movable members are connected to each other via a flat spring. A contact is disposed on the second movable member and functions as a movable contact. The external force transmission mechanism applies an external force to the first movable member to displace the first movable member, and the second movable member is reversed at the operating point of the first movable member, so that the movable contact is brought into contact with the fixed contact.

Abstract: A pressure switch includes an external force transmission mechanism for separating a cover from a housing. The cover and the external force transmission mechanism defines a first pressure chamber, and the housing and the external force transmission mechanism defines a second pressure chamber. In the second pressure chamber, a movable piece having first and second movable members arranged opposite to each other and one or more fixed contacts are disposed. The first and second movable members are connected to each other via a flat spring. A contact is disposed on the second movable member and functions as a movable contact. The external force transmission mechanism applies an external force to the first movable member to displace the first movable member, and the second movable member is reversed at the operating point of the first movable member, so that the movable contact is brought into contact with the fixed contact.

Abstract: A brush for massaging and patting skin comprising wooden base and having a concave. A bristle plant elastic base with many orifices at all around of the upper face thereof and fixed permanently to or fit repeatedly in the concave of the wooden base. And many wooden bristles can replaceably be planted in the orifices of the elastic planted base.

Abstract: A method and apparatus for pouring a plurality of materials into a plurality of containers while sequentially weighting the materials. A predetermined number of containers are arranged in a row on a pallet so that the containers are transported by a pallet as a unit. In order to pour the materials into the containers, the pallet carrying the containers is intermittently moved in a longitudinal direction by a distance corresponding to a distance between adjacent containers, and the materials are poured into the respective containers when the pallet stops with the containers at respective pouring positions. The pallet is transported in a transverse direction so as to arrange it to allow pouring of the materials into the containers and so as to discharge the pallet towards a discharge section.

Abstract: A flow sensor comprising a flow passage, a rectifying element such as a plurality of screens or a honeycomb structure or both, arranged in the flow passage, a flow passage reducer for reducing the flow passage behind the rectifying element, and a thermal flow sensor, such as for example a micro bridge sensor, mounted at a location immediately behind the reducer. The above structure can largely reduce disturbance in flow to thereby enable highly accurate measurements of a wide range of flow without a low pass filter.

Abstract: A flow control valve comprising a main valve for opening and closing a fluid conduit, an electromagnetic coil for driving the main valve, a diaphragm responsive to the action of the main valve, a sub-valve chamber partitioned by the main valve and the diaphragm, a sub-valve incorporated in the main valve for opening and closing the sub-valve chamber to a conduit in the secondary side, a projection, protrusively formed on a driving shaft of the electromagnetic coil, responsive to a driving force of the electromagnetic coil for sliding the sub-valve by a predetermined stroke to bring the same in a fully opened state with the main valve remaining in a closed state and thereafter opening the main valve, a bleed orifice provided through a side wall of the main valve for making the pressure in the sub-valve chamber equal to a primary pressure; and a returning spring for urging each of the main valve and the sub-valve in the closing direction.

Abstract: There is disclosed a proof-printing machine comprising an elongated table for mounting a printing plate thereon detachably which is supported by a base frame, a carriage means being movable along the table in the longitudinal direction hereof, a head means being mounted on the carriage means, a doctor blade means for applying ink onto the printing plate so supported by the head means as to be adjustable of its angle and contact pressure against the printing plate, a cylindrical press drum being supported by the head means so as to be rotatable about the axis thereof and adjustable of its contact pressure against the printing plate onto the peripheral surface of which a blank material to be printed is fixed, a pair of timing belt means for transferring the carriage each of which is spanned between a pair of pulleys provided at each end portion of the side wall of the table respectively, a pulse motor for driving said timing belt means, and control means for driving the pulse motor according to a predetermined