Patents by Inventor Tatsuhiko Ozawa

Tatsuhiko Ozawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20170166904
    Abstract: The purpose of the invention is to provide means with which it is possible to efficiently select a vector to which a foreign gene has been introduced when a foreign gene is to be introduced by homologous recombination to a vector having multiple sequences homologous with one another. The vector comprises, in succession, a replication origin, a sequence A, a marker gene X, two sequences C and D for introducing a foreign gene by homologous recombination, and a sequence B homologous with sequence A. The two sequences C and D are directly or indirectly adjacent to one another. The vector is used for introducing a foreign gene between the two adjacent sequences C and D.
    Type: Application
    Filed: December 7, 2016
    Publication date: June 15, 2017
    Inventors: Masae HORII, Hiroyuki KISHI, Eiji KOBAYASHI, Tatsuhiko OZAWA, Atsushi MURAGUCHI
  • Publication number: 20150203886
    Abstract: An object is to provide a TCR closing system that enables not only bias-free analysis of TCR repertoires, but also collection of antigen-specific TCR ?/? cDNA pairs and evaluation of functions thereof. There is provided a method for producing a gene of T cell receptor (TCR) specific to an antigen A, which comprises 1) the step of stimulating a group of T cells including a T cell specific to an antigen A or one T cell specific to an antigen A under a condition effective for amplification of a TCR gene; 2) the step of identifying a T cell specific to an antigen A among the group of T cells including a T cell specific to the antigen A, and sorting one T cell specific to the antigen A into a vessel; and 3) the step of subjecting the one activated T cell specific to the antigen A in the vessel to PCR to amplify a gene of TCR specific to the antigen A.
    Type: Application
    Filed: July 24, 2013
    Publication date: July 23, 2015
    Inventors: Hiroyuki Kishi, Atsushi Muraguchi, Eiji Kobayashi, Tatsuhiko Ozawa
  • Publication number: 20150038363
    Abstract: An object of the present invention is to stimulate a T cell without using a peptide/MHC tetramer. In the present invention, the step of supplying an antigen peptide to a T cell having a T cell receptor (TCR) that can recognize the antigen peptide on cell surface to form a complex of a major histocompatibility complex (MHC) molecule on the cell surface of the T cell and the antigen peptide is used, and the T cell is stimulated through recognition by TCR of the antigen peptide as the MHC molecule-antigen peptide complex on the cell surface of the same T cell. Such a stimulating and activating method would be applicable to not only T cells, but also various cells. According to the present invention, an antigen-specific T cell can be identified without establishing any antigen-specific T cell strain, and without using such a reagent as MHC/peptide tetramer. That is, a cancer-specific T cell can be efficiently and conveniently identified.
    Type: Application
    Filed: March 6, 2013
    Publication date: February 5, 2015
    Inventors: Hiroyuki Kishi, Atsushi Muraguchi, Hiroshi Hamana, Eiji Kobayashi, Tatsuhiko Ozawa
  • Publication number: 20110129816
    Abstract: A device for detecting or quantifying influenza viruses in a sample, which comprises a detection region having a human anti-influenza virus nucleoprotein antibody immobilized onto a support, a sample supply region, and a sample-migrating region; and a kit for detecting or quantifying influenza viruses, which comprises a solid phase in which a human anti-influenza virus nucleoprotein antibody is fixed onto a carrier.
    Type: Application
    Filed: June 5, 2009
    Publication date: June 2, 2011
    Applicants: NATIONAL UNIVERSITY CORPORATION UNIVERSITY OF TOYAMA, SC WORLD, INC., KYOWA MEDEX CO., LTD.
    Inventors: Atsushi Muraguchi, Hiroyuki Kishi, Tatsuhiko Ozawa, Mamoru Ikemoto, Hitoshi Ono, Kazuki Morita, Koji Uzawa