Patents by Inventor Tetsuo Ohashi

Tetsuo Ohashi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210222154
    Abstract: The present invention relates to a method for operating magnetic particles in a device in which gel-like medium layer and liquid layer are alternately arranged inside the hollow tube and, first liquid layer and second liquid layer are separated by gel-like medium layer, and magnetic solid with large particle size exists in the first liquid layer rather than magnetic particles and the said magnetic particles. The method of the present invention comprises the first step of moving the magnetic particles and the magnetic solid in the first liquid layer to the gel-like medium layer, the second step of moving the magnetic particles and the magnetic solid moved into the gel-like medium layer into the second liquid layer, and the third step of inverting the movement of the magnetic particles and the magnetic solid moved into the second liquid layer into the gel-like medium layer, by operating a magnetic field.
    Type: Application
    Filed: January 21, 2021
    Publication date: July 22, 2021
    Applicant: SHIMADZU CORPORATION
    Inventors: Akira MURAMATSU, Masamitsu SHIKATA, Masaki KANAI, Seiya FUJIWARA, Tetsuo OHASHI
  • Publication number: 20210139954
    Abstract: A pretreatment kit includes a container (10) for particle manipulation, nucleic acid capture particles (70) capable of selectively binding to nucleic acid, aqueous-phase separation medium (21, 22, 23), a plurality of kinds of aqueous liquids (35, 31, 32, 38), and a nucleic acid for individual identification. The nucleic acid for individual identification is contained in at least one of the plurality of kinds of aqueous liquids or is bound onto the surfaces of the nucleic acid capture particles. The base sequence of the nucleic acid for individual identification contains an identification sequence including a base sequence noncomplementary to the nucleic acids contained in the biological sample. The pretreatment kit is used for separating nucleic acids from biological samples that contains nucleic acids and contaminants.
    Type: Application
    Filed: January 22, 2021
    Publication date: May 13, 2021
    Applicants: SHIMADZU CORPORATION, KAZUSA DNA RESEARCH INSTITUTE
    Inventors: Masaki KANAI, Hiroyuki JIKUYA, Tetsuo OHASHI, Shin NAKAMURA, Osamu OHARA
  • Publication number: 20210129158
    Abstract: In a magnetic particle operation device 1, a plurality of liquid layers 11 and a plurality of gel-like medium layers are alternately arranged. Magnetic particles 13 are introduced into the uppermost liquid layer 11 of the magnetic particle operation device 1, and a holding magnet 60 is in contact with the outer surface of the bulging portion 21 of the container 20. Therefore, during the storage of the device 1, the magnetic particles 13 in the bulging portion 21 are aggregated and held at a position facing the holding magnet 60 in the sample introduction space by the magnetic force of the holding magnet 60. As a result, during the storage of the device 1, the magnetic particles 13 can be prevented from being brought into contact with the gel-like medium layer 12. Further, when the device 1 is used, the magnetic particles 13 can be dispersed in the liquid layer 11 by moving the holding magnet 60 away from the container 20.
    Type: Application
    Filed: April 6, 2017
    Publication date: May 6, 2021
    Inventors: Masaki KANAI, Hiroyuki JIKUYA, Tetsuo OHASHI, Seiya FUJIWARA
  • Patent number: 10941394
    Abstract: A device for manipulating magnetic particles includes a gelled medium layer and liquid layers alternately stacked in a tubular container along a longitudinal direction of the container. A magnetic particle movement portion for moving magnetic particles exists along an inner wall surface of the container, and the magnetic particle movement portion extends along the longitudinal direction of the container. At a portion where the gelled medium layer is loaded, the cross-sectional shape of the container inner wall in a plane perpendicular to the longitudinal direction of the container is non-circular, and the shape of the magnetic particle movement portion in the cross section is a curved shape or an angular shape.
    Type: Grant
    Filed: January 15, 2015
    Date of Patent: March 9, 2021
    Assignee: SHIMADZU CORPORATION
    Inventors: Masaki Kanai, Tetsuo Ohashi, Takahiro Nishimoto
  • Publication number: 20200282407
    Abstract: An operation pipe and a device equipped with the operation pipe, which use a gel to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components that are biological components such as nucleic acids. More specifically, an operation pipe and a device, with which it is possible to perform operations such as separation, extraction, purification, elution, recovery, analysis and the like of target components in a sealable pipe by operating magnetic particles in the pipe under a magnetic field from outside of the pipe.
    Type: Application
    Filed: February 5, 2020
    Publication date: September 10, 2020
    Applicant: SHIMADZU CORPORATION
    Inventors: Masamitsu SHIKATA, Ayaka MINAMIMOTO, Akira MURAMATSU, Masaki KANAI, Seiya FUJIWARA, Tetsuo OHASHI, Hiroyuki JIKUYA
  • Patent number: 10684279
    Abstract: To provide a device for operating magnetic particles capable of treating large quantity, and retaining a gel-like medium layer stably, a vessel is formed so that an area perpendicular to a longitudinal direction in a large-diameter part packed with a liquid layer is larger than an area perpendicular to the longitudinal direction in the small-diameter part packed with a gel-like medium layer. Therefore, in the vessel, it is possible to reduce the diameter of the small-diameter part while keeping the capacity of the large-diameter part large. As a result, in the vessel, it is possible to treat large quantity in the liquid layer, and it is possible to retain the gel-like medium layer stably.
    Type: Grant
    Filed: February 8, 2018
    Date of Patent: June 16, 2020
    Assignee: SHIMADZU CORPORATION
    Inventors: Masaki Kanai, Hiroyuki Jikuya, Tetsuo Ohashi, Seiya Fujiwara
  • Patent number: 10589191
    Abstract: Provided is a method for separating a substance accommodated in a vessel-shaped structure comprising subjecting a vessel-shaped structure 1, which includes a vessel portion a, a vessel portion b, and a self-fusing material X connecting the both vessel portions, and which accommodates a substance selected from the group consisting of a liquid, a solid a gas, and a dispersion system, to the steps: (i) pulling the vessel portion a and the vessel, portion b away from each other to extend the self-fusing material X; (ii) fusing the extended self-fusing material X together between the vessel portion a and the vessel portion b to separate the accommodated substance; and (iii) cutting a fused part of the self-fusing material X to separate the vessel-shaped structure 1 into a separated structure.
    Type: Grant
    Filed: May 31, 2012
    Date of Patent: March 17, 2020
    Assignee: SHIMADZU CORPORATION
    Inventors: Tetsuo Ohashi, Yukio Oikawa, Kei Shinada
  • Patent number: 10533170
    Abstract: A magnetic particle manipulation method for magnetic particle comprises the steps of; subjecting magnetic particles and a magnetic solid having a larger particle diameter than said magnetic particles to existing together in a liquid layer, and moving said magnetic solid and said magnetic particles in said liquid layer by a magnetic field manipulation. According to one aspect of the present invention; a manipulation method for magnetic particles comprising the steps of; moving magnetic particles in a first liquid layer into a gelled medium layer by a magnetic field manipulation in a device, wherein gelled medium layers and liquid layers are in-place alternately in a container, moving said magnetic particles present in the gelled medium into a second liquid layer by the magnetic field manipulation; and moving said magnetic particles along with said magnetic solid in the second liquid layer.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: January 14, 2020
    Assignee: SHIMADZU CORPORATION
    Inventors: Tetsuo Ohashi, Yukio Oikawa
  • Patent number: 10384174
    Abstract: The present invention relates to a particle manipulation method to disperse magnetic particles 70 in a liquid 35 filling up a tube container 10, wherein a circumferential direction moving step to move the magnetic particles 70 along the circumferential direction of the container 10 in the liquid 35 and in a radial direction moving step to move the magnetic particles 70 as crossing the radial direction of the container 10 in the liquid 35 are implemented repeatedly. Such manipulations can be achieved by combining rotation of the container and gravity force and magnetic field manipulations.
    Type: Grant
    Filed: November 17, 2014
    Date of Patent: August 20, 2019
    Assignees: SHIMADZU CORPORATION, KAZUSA DNA RESEARCH INSTITUTE
    Inventors: Jun Yanagibayashi, Tetsuo Ohashi, Osamu Ohara
  • Patent number: 10369563
    Abstract: Provided are a simple, quick, and low-processing cost method capable of reliably separating part of an accommodated substance accommodated in a vessel only by simple mechanical operation while maintaining a hermetically-sealed state without any contact with an outside atmosphere, as well as a vessel with which the method may be used. A separable vessel comprises a first vessel portion, a second vessel portion which is contiguous with the first vessel portion, a fracture-inducing portion formed between the first vessel portion and the second vessel portion, and a self-fusing material provided on an outer surface so as to cover the fracture-inducing portion; and may contain an accommodated substance therein.
    Type: Grant
    Filed: March 10, 2017
    Date of Patent: August 6, 2019
    Assignee: SHIMADZU CORPORATION
    Inventor: Tetsuo Ohashi
  • Publication number: 20190024142
    Abstract: A pretreatment kit includes a container (10) for particle manipulation, nucleic acid capture particles (70) capable of selectively binding to nucleic acid, aqueous-phase separation medium (21, 22, 23), a plurality of kinds of aqueous liquids (35, 31, 32, 38), and a nucleic acid for individual identification. The nucleic acid for individual identification is contained in at least one of the plurality of kinds of aqueous liquids or is bound onto the surfaces of the nucleic acid capture particles. The base sequence of the nucleic acid for individual identification contains an identification sequence including a base sequence noncomplementary to the nucleic acids contained in the biological sample. The pretreatment kit is used for separating nucleic acids from biological samples that contains nucleic acids and contaminants.
    Type: Application
    Filed: January 18, 2017
    Publication date: January 24, 2019
    Applicants: SHIMADZU CORPORATION, KAZUSA DNA RESEARCH INSTITUTE
    Inventors: Masaki KANAI, Hiroyuki JIKUYA, Tetsuo OHASHI, Shin NAKAMURA, Osamu OHARA
  • Patent number: 10159909
    Abstract: Disclosed is a particle manipulation method including steps of moving particles, which exist in a water-based liquid, into a gelled medium that is insoluble or hardly soluble in the water-based liquid in the water-based liquid, and moving the particles, which exist in the gelled medium, to the outside of the gelled medium. Preferably, the gelled medium is a gel that contains chemically crosslinking polymer. Preferably, the gelled medium has a consistency of 340 to 475. In one embodiment, the movement of the particles existing in the water-based liquid to the gelled medium and the movement of the particles existing inside the gelled medium to the water-based liquid are carried out inside a device, the device being loaded with a plurality of water-based liquids and a gelled medium interposed among the water-based liquids.
    Type: Grant
    Filed: July 3, 2013
    Date of Patent: December 25, 2018
    Assignees: SHIMADZU CORPORATION, KAZUSA DNA RESEARCH INSTITUTE
    Inventors: Tetsuo Ohashi, Osamu Ohara, Ken Nonaka
  • Patent number: 10073108
    Abstract: The present invention provides a small and low running-cost device capable of minimizing the generation of contamination sources as much as possible while performing a series of all the desired manipulations. A device for manipulating a target component in a manipulation tube, comprising: a manipulation tube comprising a tube having an optionally-closeable open end for supplying a sample containing a target component at one end and a closed end at the other end, and a manipulation medium accommodated in the tube and having a gel layer and an aqueous liquid layer multilayered in a longitudinal direction of the tube; magnetic particles that should transport the target component; and magnetic field applying means capable of applying a magnetic field to the manipulation tube to move the magnetic particles in the longitudinal direction of the tube.
    Type: Grant
    Filed: November 11, 2016
    Date of Patent: September 11, 2018
    Assignee: SHIMADZU CORPORATION
    Inventor: Tetsuo Ohashi
  • Publication number: 20180224442
    Abstract: To provide a device for operating magnetic particles capable of treating large quantity, and retaining a gel-like medium layer stably, a vessel is formed so that an area perpendicular to a longitudinal direction in a large-diameter part packed with a liquid layer is larger than an area perpendicular to the longitudinal direction in the small-diameter part packed with a gel-like medium layer. Therefore, in the vessel, it is possible to reduce the diameter of the small-diameter part while keeping the capacity of the large-diameter part large. As a result, in the vessel, it is possible to treat large quantity in the liquid layer, and it is possible to retain the gel-like medium layer stably.
    Type: Application
    Filed: February 8, 2018
    Publication date: August 9, 2018
    Applicant: SHIMADZU CORPORATION
    Inventors: Masaki KANAI, Hiroyuki JIKUYA, Tetsuo OHASHI, Seiya FUJIWARA
  • Publication number: 20180112207
    Abstract: A device for manipulating magnetic particles includes a gelled medium layer and liquid layers alternately stacked in a tubular container along a longitudinal direction of the container. A magnetic particle movement portion for moving magnetic particles exists along an inner wall surface of the container, and the magnetic particle movement portion extends along the longitudinal direction of the container. At a portion where the gelled medium layer is loaded, the cross-sectional shape of the container inner wall in a plane perpendicular to the longitudinal direction of the container is non-circular, and the shape of the magnetic particle movement portion in the cross section is a curved shape or an angular shape.
    Type: Application
    Filed: January 15, 2015
    Publication date: April 26, 2018
    Applicant: SHIMADZU CORPORATION
    Inventors: Masaki KANAI, Tetsuo OHASHI, Takahiro NISHIMOTO
  • Publication number: 20180030432
    Abstract: A device for handling of magnetic particles, in which liquids and a gel-like medium are loaded. The device is provided with: a first liquid containing part in which a first liquid is contained; a second liquid contained, part in which a second liquid is contained, a third liquid containing part in which a third liquid is contained, and a first gel-like medium containing part in which the first gel-like medium is contained. The first liquid containing part, the second liquid containing part and the third liquid containing part are connected to the first gel-like medium containing part. The first liquid, the second liquid and the third liquid are separated from each other by the first gel-like medium.
    Type: Application
    Filed: January 30, 2015
    Publication date: February 1, 2018
    Applicants: Shimadzu Corporation, KAZUSA DNA RESEARCH INSTITUTE
    Inventors: Masaki KANAI, Hiroyuki JIKUYA, Tetsuo OHASHI, Shin NAKAMURA, Osamu OHARA
  • Publication number: 20170326509
    Abstract: The present invention relates to a particle manipulation method to disperse magnetic particles 70 in a liquid 35 filling up a tube container 10, wherein a circumferential direction moving step to move the magnetic particles 70 along the circumferential direction of the container 10 in the liquid 35 and in a radial direction moving step to move the magnetic particles 70 as crossing the radial direction of the container 10 in the liquid 35 are implemented repeatedly. Such manipulations can be achieved by combining rotation of the container and gravity force and magnetic field manipulations.
    Type: Application
    Filed: November 17, 2014
    Publication date: November 16, 2017
    Applicants: SHIMADZU CORPORATION, KAZUSA DNA RESEARCH INSTITUTE
    Inventors: Jun YANAGIBAYASHI, Tetsuo OHASHI, Osamu OHARA
  • Patent number: 9714445
    Abstract: A chip device for manipulating an object component is described, in which multiple liquid substances such as reagents required for a series of manipulations on a sample are stably secured in separated states throughout the manipulations within the device. The chip device includes a manipulation chip, magnetic particles, and a magnetic field application means. The manipulation chip includes a substrate, a groove formed in the surface of the substrate, and a manipulation medium accommodated in the groove such that gel phases and aqueous liquid phases are alternately disposed in the longitudinal direction of the groove and are in contact with each other. The magnetic particles are for capturing and carrying the object component. The magnetic field application means is capable of moving the magnetic particles in the longitudinal direction of the groove in the substrate by the application of a magnetic field to the substrate.
    Type: Grant
    Filed: November 5, 2012
    Date of Patent: July 25, 2017
    Assignee: SHIMADZU CORPORATION
    Inventors: Isao Katasho, Tetsuo Ohashi
  • Publication number: 20170182488
    Abstract: Provided are a simple, quick, and low-processing cost method capable of reliably separating part of an accommodated substance accommodated in a vessel only by simple mechanical operation while maintaining a hermetically-sealed state without any contact with an outside atmosphere, as well as a vessel with which the method may be used. A separable vessel comprises a first vessel portion, a second vessel portion which is contiguous with the first vessel portion, a fracture-inducing portion formed between the first vessel portion and the second vessel portion, and a self-fusing material provided on an outer surface so as to cover the fracture-inducing portion; and may contain an accommodated substance therein.
    Type: Application
    Filed: March 10, 2017
    Publication date: June 29, 2017
    Inventor: Tetsuo Ohashi
  • Publication number: 20170152509
    Abstract: A method for operating magnetic body particles that is for fixing a target substance in a liquid sample to the surface of the magnetic body particles; and a device for operating magnetic body particles that is used for said method. The magnetic body particles are particles to which a target substance can be fixed selectively. In this method, in a state where a liquid sample, the magnetic body particles, and a magnetic solid body that has a larger particle diameter than that of the magnetic body particle are made to coexist in a container, the magnetic body particles are moved together with the magnetic solid body within the liquid sample by operating a magnetic field from outside the container. By this operation, the target substance can be fixed selectively to the surface of the magnetic body particles.
    Type: Application
    Filed: May 23, 2014
    Publication date: June 1, 2017
    Applicant: Shimadzu Corporation
    Inventor: Tetsuo OHASHI