Patents by Inventor Tetsushi Sakuma

Tetsushi Sakuma has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10907589
    Abstract: A work vehicle includes an engine, a particulate filter, and an oil separator. The particulate filter is connected to the engine to collect particulate matters contained in exhaust gas from the engine. The particulate filter is provided to overlap with the engine when viewed in a height direction along a height of the work vehicle. The oil separator is connected to the engine via a blow-by-gas discharge pipe to catch a liquid component in blow-by-gas from the engine. The oil separator is provided between the engine and the particulate filter to overlap with the particulate filter when viewed in the height direction.
    Type: Grant
    Filed: November 25, 2019
    Date of Patent: February 2, 2021
    Assignee: KUBOTA CORPORATION
    Inventors: Tetsushi Sakuma, Akiyoshi Ono, Megumi Sawai, Daisuke Inaba, Shinji Kato
  • Publication number: 20200362323
    Abstract: The present disclosure provides a technique whereby the influence of an endogenous TCR is eliminated in TCR gene transfer. A TCR gene is edited using a genome editing enzyme, said genome editing enzyme having one characteristic that amino acids at two specific positions in DNA-binding modules contained in a DNA-binding domain thereof show repeating patterns which differ from one module to another among the four DNA-binding modules. Thus, a lowering in the expression efficiency of the transferred TCR caused by mispairing with an endogenous TCR and the occurrence of a self-reactive TCR are avoided.
    Type: Application
    Filed: October 9, 2018
    Publication date: November 19, 2020
    Inventors: Tatsuo Ichinohe, Takashi Yamamoto, Tetsushi Sakuma, Yasuko Honjo, Takakazu Kawase, Takahiko Miyama, Ryuji Suzuki
  • Publication number: 20200318125
    Abstract: It is an object of the present invention to provide a method for introducing a protein into a plant, which is simple and extensively applicable to various types of plant cells and proteins. The above object is achieved by the present invention to provide a complex comprising a protein of interest to be introduced into a target plant cell and a carrier peptide, a method for introducing a protein of interest into a target plant cell using the complex, and a kit comprising a protein of interest to be introduced into a target plant cell and a carrier peptide.
    Type: Application
    Filed: January 19, 2017
    Publication date: October 8, 2020
    Applicant: RIKEN
    Inventors: Keiji NUMATA, Yoko HORII, Takeshi YOSHIZUMI, Taku DEMURA, Yutaka KODAMA, Takashi YAMAMOTO, Tetsushi SAKUMA, Kenji MIURA, Hiroshi EZURA
  • Publication number: 20200200128
    Abstract: A work vehicle includes an engine, a particulate filter, and an oil separator. The particulate filter is connected to the engine to collect particulate matters contained in exhaust gas from the engine. The particulate filter is provided to overlap with the engine when viewed in a height direction along a height of the work vehicle. The oil separator is connected to the engine via a blow-by-gas discharge pipe to catch a liquid component in blow-by-gas from the engine. The oil separator is provided between the engine and the particulate filter to overlap with the particulate filter when viewed in the height direction.
    Type: Application
    Filed: November 25, 2019
    Publication date: June 25, 2020
    Applicant: Kubota Corporation
    Inventors: Tetsushi SAKUMA, Akiyoshi ONO, Megumi SAWAI, Daisuke INABA, Shinji KATO
  • Publication number: 20200079257
    Abstract: A seat suspension includes a base including a base hook and a seat platform provided with a seat. A first link couples a first rotational axis of the base and a third rotational axis of the seat platform and is configured to be rotatable about the first rotational axis and the third rotational axis. A second link couples a second rotational axis of the base and a fourth rotational axis of the seat platform and is configured to be rotatable about the second rotational axis and the fourth rotational axis. A link hook is provided on the second link. An elastic member is latched to the base hook and the link hook and is configured to pull the link hook toward the base hook. A first angle between a contracting direction of the elastic member and a rotation direction of the link hook increases as the elastic member expands.
    Type: Application
    Filed: September 9, 2019
    Publication date: March 12, 2020
    Applicant: Kubota Corporation
    Inventors: Tetsushi SAKUMA, Daisuke YASUNOBE, Takateru NAKAGAWA
  • Publication number: 20190330280
    Abstract: [Problem to be Solved] It is intended to provide a fusion polypeptide that regulates the transcription of a target gene. [Solution] The present inventors have provided a fusion polypeptide comprising: a cell-penetrating peptide; a DNA-binding polypeptide; and a transcriptional regulator.
    Type: Application
    Filed: December 11, 2017
    Publication date: October 31, 2019
    Applicants: Astellas Pharma Inc., National Center for Global Health and Medicine, Hiroshima University
    Inventors: Hideki ENDO, Yukihito ISHIZAKA, Takashi YAMAMOTO, Tetsushi SAKUMA
  • Publication number: 20190177745
    Abstract: The present invention provides the following: a vector for inserting a desired nucleic acid into a predetermined site of a nucleic acid comprising a region formed of a first nucleotide sequence, the predetermined site, and a region composed of a second nucleotide sequence, in the stated order in the 5?-to-3? direction, wherein the vector comprises a region formed of the first nucleotide sequence, the desired nucleic acid, and the second nucleotide sequence in the stated order in the 5?-to-3? direction; a kit that includes this vector; a method of inserting a nucleic acid comprising a step for introducing this vector into a cell; a cell acquired by this method; and an organism comprising this cell.
    Type: Application
    Filed: February 22, 2019
    Publication date: June 13, 2019
    Applicant: HIROSHIMA UNIVERSITY
    Inventors: Takashi Yamamoto, Kenichi Suzuki, Tetsushi Sakuma, Yuto Sakane
  • Publication number: 20190169240
    Abstract: The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an ?-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an ?-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (?2) A.A.
    Type: Application
    Filed: December 11, 2018
    Publication date: June 6, 2019
    Applicants: KYUSHU UNIVERSITY, NAT'L UNIVERSITY CORPORATION, HIROSHIMA UNIVERSITY
    Inventors: Takashi Yamamoto, Tetsushi Sakuma, Takahiro Nakamura, Yusuke Yagi, Yasuyuki Okawa
  • Patent number: 10189879
    Abstract: The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an ?-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an ?-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (?2) A. A.
    Type: Grant
    Filed: April 22, 2014
    Date of Patent: January 29, 2019
    Assignees: KYUSHU UNIVERSITY, NAT'L UNIVERSITY CORPORATION, HIROSHIMA UNIVERSITY
    Inventors: Takashi Yamamoto, Tetsushi Sakuma, Takahiro Nakamura, Yusuke Yagi, Yasuyuki Okawa
  • Publication number: 20180273921
    Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.
    Type: Application
    Filed: June 6, 2018
    Publication date: September 27, 2018
    Applicant: Hiroshima University
    Inventors: Takashi Yamamoto, Tetsushi Sakuma, Hiroshi Ochiai, Shinya Matsuura, Tatsuo Miyamoto
  • Publication number: 20180242562
    Abstract: According to this invention, a bird lays an egg that does not contain an artificially introduced foreign gene in genome thereof and has an ovomucoid content lower than that in a wild type thereof. A method for producing a bird includes a modification step of cleaving the ovomucoid gene locus in a pluripotent stem cell of a bird with programmable endonuclease so as to modify the ovomucoid gene locus and a transplantation step of transplanting the pluripotent stem cell in which the ovomucoid gene locus is modified into an embryo of a bird. A bird egg does not contain an artificially introduced foreign gene in genome thereof and has an ovomucoid content lower than that in a wild type thereof.
    Type: Application
    Filed: July 20, 2016
    Publication date: August 30, 2018
    Inventors: Hiroyuki Horiuchi, Ryo Ezaki, Takashi Yamamoto, Tetsushi Sakuma, Akihiro Handa, Ryou Sasahara, Daisuke Kodama
  • Patent number: 10030235
    Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.
    Type: Grant
    Filed: May 9, 2014
    Date of Patent: July 24, 2018
    Assignee: Hiroshima University
    Inventors: Takashi Yamamoto, Tetsushi Sakuma, Hiroshi Ochiai, Shinya Matsuura, Tatsuo Miyamoto
  • Patent number: 10006011
    Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.
    Type: Grant
    Filed: February 9, 2016
    Date of Patent: June 26, 2018
    Assignee: Hiroshima University
    Inventors: Takashi Yamamoto, Tetsushi Sakuma, Hiroshi Ochiai, Shinya Matsuura, Tatsuo Miyamoto
  • Publication number: 20170248896
    Abstract: Image forming apparatus, including: image bearer; charging unit for electrically charging image bearer surface; developing unit for developing with toner, electrostatic latent image formed over image bearer by exposure unit for performing light exposure; transfer unit for transferring developed toner to receiving member; and cleaning unit for cleaning toner remaining over image bearer without being transferred, wherein the toner contains: external additives; and base particles made of at least binder resin and colorant, external additive content of the toner is from 4 to 7 parts by mass relative to 100 parts by mass of base particles, primary particles of at least one external additive have a number average particle diameter of 0.01 to 0.05 ?m, cleaning unit includes an elastic-body blade having surface elastic modulus of from 15 to 25 N/mm2 and surface friction coefficient of from 0.5 to 0.7 at an abutment part thereof abutting on image bearer.
    Type: Application
    Filed: July 17, 2015
    Publication date: August 31, 2017
    Inventors: Naoki NAKATAKE, Takeshi YAMASHITA, Tetsushi SAKUMA, Mitsutoshi KICHISE, Tsuyoshi NOZAKI, Yoshihiro MIKURIYA, Yoshimichi ISHIKAWA, Atsushi YAMAMOTO, Kazuoki FUWA, Tomohiro FUKAO, Tomoharu MIKI
  • Publication number: 20160264995
    Abstract: The present invention provides the following: a vector for inserting a desired nucleic acid into a predetermined site of a nucleic acid comprising a region formed of a first nucleotide sequence, the predetermined site, and a region composed of a second nucleotide sequence, in the stated order in the 5?-to-3? direction, wherein the vector comprises a region formed of the first nucleotide sequence, the desired nucleic acid, and the second nucleotide sequence in the stated order in the 5?-to-3? direction; a kit that includes this vector; a method of inserting a nucleic acid comprising a step for introducing this vector into a cell; a cell acquired by this method; and an organism comprising this cell.
    Type: Application
    Filed: October 30, 2014
    Publication date: September 15, 2016
    Applicant: HIROSHIMA UNIVERSITY
    Inventors: Takashi Yamamoto, Kenichi Suzuki, Tetsushi Sakuma, Yuto Sakane
  • Publication number: 20160186151
    Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.
    Type: Application
    Filed: May 9, 2014
    Publication date: June 30, 2016
    Applicant: HIROSHIMA UNIVERSITY
    Inventors: Takashi Yamamoto, Tetsushi Sakuma, Hiroshi Ochiai, Shinya Matsuura, Tatsuo Miyamoto
  • Patent number: 9360824
    Abstract: An image forming apparatus is provided which includes an image bearer, a charger, an irradiator, a developing device to develop an electrostatic latent image on the image bearer with a toner to form a toner image, a transfer device to transfer the toner image onto a transfer medium, and a cleaner to remove toner particles remaining on the image bearer without being transferred. The toner includes a mother particle including a binder resin and a colorant and one or more external additives. At least one of the external additives includes primary particles having a number average particle diameter in the range of 0.05 to 0.30 ?m. The cleaner includes an elastic body blade having a contact part with the image bearer. The contact part has a surface elastic modulus in the range of 15 to 25 N/mm2 and a surface friction coefficient in the range of 0.5 to 0.7.
    Type: Grant
    Filed: July 6, 2015
    Date of Patent: June 7, 2016
    Assignee: Ricoh Company, Ltd.
    Inventors: Tomohiro Fukao, Tsuyoshi Nozaki, Yoshihiro Mikuriya, Yoshimichi Ishikawa, Atsushi Yamamoto, Kazuoki Fuwa, Tomoharu Miki, Naoki Nakatake, Takeshi Yamashita, Tetsushi Sakuma
  • Publication number: 20160152990
    Abstract: The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library.
    Type: Application
    Filed: February 9, 2016
    Publication date: June 2, 2016
    Applicant: HIROSHIMA UNIVERSITY
    Inventors: Takashi Yamamoto, Tetsushi Sakuma, Hiroshi Ochiai, Shinya Matsuura, Tatsuo Miyamoto
  • Publication number: 20160075744
    Abstract: The object of the present invention is to, by analyzing PPR proteins that act to bind to DNA with a prediction that RNA recognition rules of PPR motifs can also be used for recognition of DNA, find a PPR protein showing such a characteristic. According to the present invention, it was revealed that, with a protein that can bind in a DNA base-selective manner or a DNA base sequence-specific manner, which contains one or more, preferably 2 to 30, more preferably 5 to 25, most preferably 9 to 15, of PPR motifs having a structure of the following formula 1 (wherein, in the formula 1, Helix A is a part that can form an ?-helix structure; X does not exist, or is a part consisting of 1 to 9 amino acids; Helix B is a part that can form an ?-helix structure; and L is a part consisting of 2 to 7 amino acids), and having a specific combination of amino acids corresponding to a DNA base or DNA base sequence as amino acids of three positions of No. 1 A.A., No. 4 A.A., in Helix A of the formula 1 and No. “ii” (-2) A.A.
    Type: Application
    Filed: April 22, 2014
    Publication date: March 17, 2016
    Applicants: KYUSHU UNIVERSITY, NATATIONAL UNIVERSITY CORPORATION, HIROSHIMA UNIVERSITY
    Inventors: Takashi Yamamoto, Tetsushi Sakuma, Takahiro Nakamura, Yusuke Yagi, Yasuyuki Okawa
  • Publication number: 20160033924
    Abstract: An image forming apparatus is provided which includes an image bearer, a charger, an irradiator, a developing device to develop an electrostatic latent image on the image bearer with a toner to form a toner image, a transfer device to transfer the toner image onto a transfer medium, and a cleaner to remove toner particles remaining on the image bearer without being transferred. The toner includes a mother particle including a binder resin and a colorant and one or more external additives. At least one of the external additives includes primary particles having a number average particle diameter in the range of 0.05 to 0.30 ?m. The cleaner includes an elastic body blade having a contact part with the image bearer. The contact part has a surface elastic modulus in the range of 15 to 25 N/mm2 and a surface friction coefficient in the range of 0.5 to 0.7.
    Type: Application
    Filed: July 6, 2015
    Publication date: February 4, 2016
    Inventors: Tomohiro FUKAO, Tsuyoshi NOZAKI, Yoshihiro MIKURIYA, Yoshimichi ISHIKAWA, Atsushi YAMAMOTO, Kazuoki FUWA, Tomoharu MIKI, Naoki NAKATAKE, Takeshi YAMASHITA, Tetsushi SAKUMA