Patents by Inventor Theresa C. White
Theresa C. White has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 10364422Abstract: Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.Type: GrantFiled: January 25, 2016Date of Patent: July 30, 2019Assignee: IOGEN ENERGY CORPORATIONInventors: Brian R. Scott, Patrick St-Pierre, James Lavigne, Nabil Masri, Theresa C. White, John J. Tomashek
-
Publication number: 20160201043Abstract: Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.Type: ApplicationFiled: January 25, 2016Publication date: July 14, 2016Inventors: Brian R. Scott, Patrick St-Pierre, James Lavigne, Nabil Masri, Theresa C. White, John J. Tomashek
-
Patent number: 9279112Abstract: Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acid sequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolyzing cellulose with the modified cellulases in the presence of lignin.Type: GrantFiled: February 26, 2010Date of Patent: March 8, 2016Assignee: IOGEN ENERGY CORPORATIONInventors: Brian R. Scott, Patrick St-Pierre, James Lavigne, Nabil Masri, Theresa C. White, John J. Tomashek
-
Patent number: 8323931Abstract: A fermentation process for the production of cellulase mixtures is provided. The process comprises providing a genetically modified host filamentous fungus that overexpresses a Xyr1 transcription factor and/or that is partially or completely deficient in expressing one or more hemicellulase enzyme. The host filamentous fungus is cultured in a medium comprising a carbon source. The carbon source contains from about 60 wt % to about 100 wt % hemicellulose-derived carbohydrate and less than 5% of a cellulase-inducing carbohydrate or contains from about 25 wt % to about 100% wt % hemicellulose-derived sugar alcohol in combination with from about 0 wt % to about 75 wt % glucose, glycerol or a combination thereof.Type: GrantFiled: November 3, 2009Date of Patent: December 4, 2012Assignee: Iogen Energy CorporationInventors: Loreta Gudynaite-Savitch, Christopher D. Hindle, Theresa C. White
-
Patent number: 8101398Abstract: A modified Family 6 cellulase enzyme comprising a proline residue at position 413 is provided. Genetic constructs and genetically modified microbes comprising DNA sequences encoding the modified Family 6 cellulase are also provided. Family 6 cellulases of the invention display improved thermostability, thermophilicity, alkalophilicity, or a combination thereof, relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry that require cellulase stability and activities at temperatures, pH values, or both, above that of the native enzyme.Type: GrantFiled: June 23, 2010Date of Patent: January 24, 2012Assignee: Iogen Energy CorporationInventors: Patrick St-Pierre, Nabil Masri, Marie-Christine Fournier, Theresa C. White
-
Patent number: 8012721Abstract: The present invention pertains to a method of converting cellulose to glucose by treating a pretreated lignocellulosic substrate with an enzyme mixture comprising cellulase enzyme and endoglucanase core proteins, wherein the endoglucanase core proteins are present in the enzyme mixture at an amount relative to all endoglucanases from about 35 wt. % to about 100 wt. % and wherein the endoglucanase cellulase enzymes are present in the enzyme mixture at an amount relative to the amount of CBH and EG enzymes from about 2 wt. % to about 50 wt. %. The pretreated lignocellulosic substrate is selected from the group consisting of agricultural residues, residues after starch or sugar removal, dedicated ethanol crops, forestry products, and pulp and paper products, or combinations thereof.Type: GrantFiled: March 5, 2003Date of Patent: September 6, 2011Assignee: Iogen Energy CorporationInventors: Daphne Wahnon, Theresa C. White, Jennifer Donaldson, Jeffrey S. Tolan
-
Publication number: 20100317087Abstract: A modified Family 6 cellulase enzyme comprising a proline residue at position 413 is provided. Genetic constructs and genetically modified microbes comprising DNA sequences encoding the modified Family 6 cellulase are also provided. Family 6 cellulases of the invention display improved thermostability, thermophilicity, alkalophilicity, or a combination thereof, relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry that require cellulase stability and activities at temperatures, pH values, or both, above that of the native enzyme.Type: ApplicationFiled: June 23, 2010Publication date: December 16, 2010Applicant: IOGEN ENERGY CORPORATIONInventors: Patrick St-Pierre, Nabil Masri, Marie-Christine Fournier, Theresa C. White
-
Publication number: 20100221778Abstract: Provided are modified cellulase enzymes exhibiting increase cellulose-hydrolyzing activity in the presence of lignin and/or reduced binding to lignin comprising modified linker peptides comprising one or more amino acid substitutions, insertions, or deletions that result in (a) a decrease in the calculated isoelectric point of the linker peptide and/or (b) an increase in the ratio of threonine:serine in the linker peptide relative to a parental linker peptide from which said modified linker peptide is derived. Also provided are genetic constructs comprising nucleic acidsequences encoding for modified cellulase enzymes, methods for the production of the modified cellulase enzymes from host strains and a process for hydrolysing cellulose with the modified cellulases in the presence of lignin.Type: ApplicationFiled: February 26, 2010Publication date: September 2, 2010Applicant: IOGEN ENERGY CORPORATIONInventors: Brian R. Scott, Patrick St-Pierre, James Lavigne, Nabil Masri, Theresa C. White, John J. Tomashek
-
Patent number: 7785854Abstract: A modified Family 6 cellulase enzyme comprising a proline residue at position 413 is provided. Genetic constructs and genetically modified microbes comprising DNA sequences encoding the modified Family 6 cellulase are also provided. Family 6 cellulases of the invention display improved thermostability, thermophilicity, alkalophilicity, or a combination thereof, relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry that require cellulase stability and activities at temperatures, pH values, or both, above that of the native enzyme.Type: GrantFiled: August 29, 2007Date of Patent: August 31, 2010Assignee: Iogen Energy CorporationInventors: Patrick St-Pierre, Nabil Masri, Marie-Christine Fournier, Theresa C. White
-
Publication number: 20100129880Abstract: A fermentation process for the production of cellulase mixtures is provided. The process comprises providing a genetically modified host filamentous fungus that overexpresses a Xyr1 transcription factor and/or that is partially or completely deficient in expressing one or more hemicellulase enzyme. The host filamentous fungus is cultured in a medium comprising a carbon source. The carbon source contains from about 60 wt % to about 100 wt % hemicellulose-derived carbohydrate and less than 5% of a cellulase-inducing carbohydrate or contains from about 25 wt % to about 100% wt % hemicellulose-derived sugar alcohol in combination with from about 0 wt % to about 75 wt % glucose, glycerol or a combination thereof.Type: ApplicationFiled: November 3, 2009Publication date: May 27, 2010Applicant: IOGEN ENERGY CORPORATIONInventors: Loreta Gudynaite-Savitch, Christopher D. Hindle, Theresa C. White
-
Patent number: 7691609Abstract: A modified Family 11 xylanase enzyme comprising a sequence that introduces a functional consensus glycosylation site is provided. Non-limiting examples of introduced glycosylation sites include mutation of the amino acid at position 34, 131, 180, 182, or a combination thereof, to an asparagine. The indicated amino acid position in the Family 11 xylanase is determined from sequence alignment of the xylanase of interest with that of a Trichoderma reesei xylanase II amino acid sequence. The introduced consensus glycosylation site facilitates increased expression efficiency of the modified xylanase when compared to the expression efficiency of a corresponding xylanase from which the modified xylanase was derived, using similar host strains and growth conditions.Type: GrantFiled: November 7, 2008Date of Patent: April 6, 2010Assignee: Iogen Bio-Products CorporationInventors: Theresa C. White, Genevieve R. Giroux, Katie E. A. Wallace
-
Patent number: 7419809Abstract: The present invention pertains to a method of converting cellulose to glucose by treating a pretreated lignocellulosic substrate with an enzyme mixture comprising cellulase enzyme and a modified CBHO, wherein the modified CBHI is present in the enzyme mixture at an amount relative to all CBHI-type enzymes from about 15% to about 100% (w/w),depending upon the modified CBHI used. The pretreated lignocellulosic substrate is selected from the group consisting of agricultural residues, residues after starch or sugar removal dedicated ethanol crops forestry products, and pulp and paper products, or combinations thereof.Type: GrantFiled: September 25, 2001Date of Patent: September 2, 2008Assignee: Iogen Energy CorporationInventors: Brian Foody, Theresa C. White, Jeffrey S. Tolan, Jennifer Donaldson
-
Patent number: 6939704Abstract: The present invention relates to increasing the production of a protein of interest from a fugal host. The invention discloses nucleotide sequences comprising, a regulatory region in operative association with xylanase secretion sequence and a gene of interest. The gene of interest encodes a protein selected from a pharmaceutical, nutraceutical, industrial, animal feed, food additive and an enzyme. Preferably, the gene of interest encodes a cellulase, hernicellulase, a lignin degrading enzyme, pectinase, protease, or peroxidase. The present invention also relates to vectors and hosts comprising these nucleic acid sequences, and to methods for the production of a protein of interest.Type: GrantFiled: September 9, 1999Date of Patent: September 6, 2005Assignee: Iogen Energy CorporationInventors: Theresa C. White, Sylvia McHugh, Christopher D. Hindle
-
Publication number: 20040053373Abstract: The present invention pertains to a method of converting cellulose to glucose by treating a pretreated lignocellulosic substrate with an enzyme mixture comprising cellulase enzyme and a modified CBHI, wherein the modified CBHI is present in the enzyme mixture at an amount relative to all CBHI-type enzymes from about 15% to about 100% (w/w), dpending upon the modified CBH1 used. The pretreated lignocellulosic substrate is selected from the group consisting of agricultural residues, residues after starch or sugar removal, dedicated ethanol crops, forestry products, and pulp and paper products, or combinations thereof.Type: ApplicationFiled: September 24, 2003Publication date: March 18, 2004Inventors: Brian Foody, Theresa C. White, Jeffrey S. Tolan, Jennifer Donaldson
-
Patent number: 6015703Abstract: This invention relates to the genetic modification of a microbe to enhance its production of an enzyme, beta-glucosidase, that is important in the cellulose conversion process. The inventors have discovered genetic constructs that, when expressed in recombinant microbes, dramatically increase the amount of beta-glucosidase produced relative to untransformed microbes. The genetic constructs comprise a promoter, a xylanase secretion signal, and a mature beta-glucosidase coding region. The increased level of beta-glucosidase significantly increases the efficiency of hydrolysis of cellulose to glucose by cellulase enzymes, thereby enhancing the production of fuel ethanol from cellulose.Type: GrantFiled: March 10, 1998Date of Patent: January 18, 2000Assignee: Iogen CorporationInventors: Theresa C. White, Christopher D. Hindle