Patents by Inventor Tobias William Barr Ost

Tobias William Barr Ost has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8945835
    Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
    Type: Grant
    Filed: April 11, 2012
    Date of Patent: February 3, 2015
    Assignee: Illumina Cambridge Limited
    Inventors: Eric Hans Vermaas, Graham John Worsley, Jonathan Mark Boutell, Colin Lloyd Barnes, Roberto Rigatti, Niall Anthony Gormley, Geoffrey Paul Smith, Vincent Peter Smith, Tobias William Barr Ost, David Bentley
  • Publication number: 20140349891
    Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction,
    Type: Application
    Filed: January 28, 2014
    Publication date: November 27, 2014
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Xiaohai Liu, John Milton, Geoffrey Paul Smith, Colin Lloyd Barnes, Isabelle Marie Julia Rasolonjatovo, Roberto Rigatti, Xiaolin Wu, Tobias William Barr Ost, Graham John Worsley, David James Earnshaw, Gerardo Turcatti, Anthony Romieu
  • Publication number: 20140336057
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Application
    Filed: May 19, 2014
    Publication date: November 13, 2014
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Roberto Rigatti, Tobias William Barr Ost
  • Publication number: 20140287415
    Abstract: Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.
    Type: Application
    Filed: December 20, 2013
    Publication date: September 25, 2014
    Applicant: ILLUMINA, INC.
    Inventors: Geoffrey Paul Smith, Roberto Rigatti, Tobias William Barr Ost, Shankar Balasubramanian, Raquel Maria Sanches-Kuiper
  • Patent number: 8765381
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Grant
    Filed: April 4, 2013
    Date of Patent: July 1, 2014
    Assignee: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Tobias William Barr Ost
  • Patent number: 8715966
    Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
    Type: Grant
    Filed: April 19, 2012
    Date of Patent: May 6, 2014
    Assignee: Illumina Cambridge Limited
    Inventors: Liu Xiaohai, John Milton, Geoffrey Paul Smith, Colin Lloyd Barnes, Isabelle Marie Julia Rasolonjatovo, Roberto Rigatti, Xiaolin Wu, Tobias William Barr Ost, Graham John Worsley, David James Earnshaw, Gerardo Turcatti, Anthony Romieu
  • Patent number: 8623628
    Abstract: Modified DNA polymerases have an affinity for DNA such that the polymerase has an ability to incorporate one or more nucleotides into a plurality of separate DNA templates in each reaction cycle. The polymerases are capable of forming an increased number of productive polymerase-DNA complexes in each reaction cycle. The modified polymerases may be used in a number of DNA sequencing applications, especially in the context of clustered arrays.
    Type: Grant
    Filed: May 10, 2006
    Date of Patent: January 7, 2014
    Assignee: Illumina, Inc.
    Inventors: Tobias William Barr Ost, Geoffrey Paul Smith, Shankar Balasubramanian, Roberto Rigatti, Raquel Maria Sanches
  • Publication number: 20130165327
    Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
    Type: Application
    Filed: April 11, 2012
    Publication date: June 27, 2013
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Eric Hans Vermaas, Graham John Worsley, Jonathan Mark Boutell, Colin Lloyd Barnes, Roberto Rigatti, Niall Anthony Gormley, Geoffrey Paul Smith, Vincent Peter Smith, Tobias William Barr Ost, David Bentley
  • Patent number: 8431348
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Grant
    Filed: June 29, 2012
    Date of Patent: April 30, 2013
    Assignee: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Tobias William Barr Ost
  • Publication number: 20120309634
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Application
    Filed: June 29, 2012
    Publication date: December 6, 2012
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Roberto Rigatti, Tobias William Barr Ost
  • Publication number: 20120208194
    Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction,
    Type: Application
    Filed: April 19, 2012
    Publication date: August 16, 2012
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Xiaohai Liu, John Milton, Geoffrey Paul Smith, Colin Barnes, Isabelle Marie Julia Rasolonjatovo, Roberto Rigatti, Xiaolin Wu, Tobias William Barr Ost, Graham John Worsley, David James Earnshaw, Gerardo Turcatti, Anthony Romieu
  • Patent number: 8236505
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Grant
    Filed: December 19, 2011
    Date of Patent: August 7, 2012
    Assignee: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Tobias William Barr Ost
  • Patent number: 8192930
    Abstract: The invention provides methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template.
    Type: Grant
    Filed: February 8, 2007
    Date of Patent: June 5, 2012
    Assignee: Illumina Cambridge Limited
    Inventors: Eric Hans Vermaas, Graham John Worsley, Jonathan Mark Boutell, Colin Lloyd Barnes, Roberto Rigatti, Niall Anthony Gormley, Geoffrey Paul Smith, Vincent Peter Smith, Tobias William Barr Ost, David Bentley
  • Publication number: 20120094848
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Application
    Filed: December 19, 2011
    Publication date: April 19, 2012
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Roberto Rigatti, Tobias William Barr Ost
  • Patent number: 8105784
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Grant
    Filed: May 3, 2011
    Date of Patent: January 31, 2012
    Assignee: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Tobias William Barr Ost
  • Publication number: 20110223601
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Application
    Filed: May 3, 2011
    Publication date: September 15, 2011
    Applicant: ILLUMINA CAMBRIDGE LIMITED
    Inventors: Roberto Rigatti, Tobias William Barr Ost
  • Patent number: 7960120
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Grant
    Filed: April 1, 2010
    Date of Patent: June 14, 2011
    Assignee: Illumina Cambridge Ltd.
    Inventors: Roberto Rigatti, Tobias William Barr Ost, Sarah J. Fashena
  • Publication number: 20110014657
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Application
    Filed: April 1, 2010
    Publication date: January 20, 2011
    Inventors: Roberto Rigatti, Tobias William Barr Ost, Sarah J. Fashena
  • Patent number: 7754429
    Abstract: The invention relates to methods for pairwise sequencing of a double-stranded polynucleotide template, which methods result in the sequential determination of nucleotide sequences in two distinct and separate regions of the polynucleotide template. Using the methods of the invention it is possible to obtain two linked or paired reads of sequence information from each double-stranded template on a clustered array, rather than just a single sequencing read from one strand of the template.
    Type: Grant
    Filed: October 5, 2007
    Date of Patent: July 13, 2010
    Assignee: Illumina Cambridge Limited
    Inventors: Roberto Rigatti, Tobias William Barr Ost
  • Publication number: 20090118128
    Abstract: The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5? end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction.
    Type: Application
    Filed: July 20, 2006
    Publication date: May 7, 2009
    Inventors: Xiaohai Liu, John Milton, Geoffrey Paul Smith, Colin Barnes, Isabelle Marie Julia Rasolonjatovo, Roberto Rigatti, Xiaolin Wu, Tobias William Barr Ost, Graham John Worsley, David James Earnshaw, Gerardo Turcatti, Anthony Romieu