Patents by Inventor Tomohisa Hasunuma

Tomohisa Hasunuma has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210292149
    Abstract: A cap attaching/detaching device 20 is a device for attaching or detaching a cap 141 in which a screw portion is formed to or from a test tube 14 by rotating the cap 141. A cap grip portion 21 grips the cap 141. A rotation mechanism 22 attaches or detaches the cap 141 to or from the test tube 14 by rotating the cap 141 by rotating the cap grip portion 21. The rotation mechanism 22 has an axial portion 221, and a nut portion 222. The axial portion 221 has an outer peripheral surface on which a screw thread 223 is formed. The nut portion 222 has an inner peripheral surface on which a screw groove 224 to be screwed into the screw thread 223 of the axial portion 221 is formed, and rotatably holds the axial portion 221 in a fixed state. A pitch of the screw thread 223 is the same as a pitch of the screw portion formed in the cap 141.
    Type: Application
    Filed: July 10, 2019
    Publication date: September 23, 2021
    Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, SHIMADZU CORPORATION
    Inventors: Tsutomu WATANABE, Akari ODA, Tomohisa HASUNUMA
  • Publication number: 20210292705
    Abstract: When a stirring device 11 performs processing of stirring a mixed solution in a test tube S, a holding mechanism 13 and a movement mechanism 14 are operated so that the test tube S is moved to a position of B2. Then, in the test tube S, the cells attached to the inner surface of the test tube S and the liquid are separated. Further, the cells attached to the inner surface of the test tube S are detected by a color sensor 152. Therefore, it is possible to reduce the work for determining the end of the stirring processing by the user. As a result, the workability of the user when using the stirring device 11 can be improved.
    Type: Application
    Filed: July 10, 2019
    Publication date: September 23, 2021
    Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, SHIMADZU CORPORATION
    Inventors: Aya TORAI, Yoshihiro MATSUMOTO, Tomohisa HASUNUMA
  • Publication number: 20210292698
    Abstract: A stirring device 11 includes a stirring mechanism 7 and a control unit 15. The stirring mechanism 7 includes a motor 74 and a pedestal portion 77. In the stirring device 11, the motor 74 repeats forward rotation and reverse rotation alternately under the control of the control unit 15. Then, the pedestal portion 77 repeats turning in one direction and turning in the other direction alternately. As a result, the test tube S repeats the movement in one direction and the movement in the other direction alternately, and the vortex rotating in one direction and the vortex rotating in the other direction are alternately generated in the mixed solution in the test tube S. Then, the mixed solution in the test tube S is stirred to be rotated, and is stirred to be moved in the vertical direction. Therefore, the mixed solution in the test tube S can be efficiently stirred. As a result, the time required for stirring the mixed solution in the test tube S can be shortened.
    Type: Application
    Filed: July 10, 2019
    Publication date: September 23, 2021
    Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, SHIMADZU CORPORATION
    Inventors: Aya TORAI, Yoshihiro MATSUMOTO, Tomohisa HASUNUMA
  • Publication number: 20210295526
    Abstract: A solid-liquid boundary detection device 20 is a device for detecting a boundary 141 between a solid and a liquid in a test tube 14, and includes a surface illumination 21, an imaging unit 22, and a boundary detection processor 31. The surface illumination 21 illuminates the test tube 14 from outside. The imaging unit 22 is arranged on an opposite side of the test tube 14 from the surface illumination 21, and captures an image including the boundary 141. The boundary detection processor 31 detects the boundary 141 from the image captured by the imaging unit 22.
    Type: Application
    Filed: July 10, 2019
    Publication date: September 23, 2021
    Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, SHIMADZU CORPORATION
    Inventors: Tsutomu WATANABE, Naoki TAKAMURA, Tomohisa HASUNUMA
  • Publication number: 20200270566
    Abstract: A method of cultivating algal cells of an algae belonging to a class selected from Chlorophyceae, Euglenophyceae, Bacillariophyceae and Haptophyceae includes: irradiating the algal cells with an artificial light having a ratio of (i) photon flux density in a wavelength range of 520-630 nm to (ii) photosynthetic photon flux density, that is 65% or more; and measuring a condition of the algal cells and/or a condition of an algal cell culture provided by cultivating the algal cells. Irradiation and non-irradiation of the algal cells with the artificial light are switched, or the photon flux density in the wavelength range of 520-630 nm is changed, according to the measured condition of the algal cells and/or the measured condition of the algal cell culture.
    Type: Application
    Filed: February 26, 2020
    Publication date: August 27, 2020
    Applicants: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY, NICHIA CORPORATION
    Inventors: Tomohisa HASUNUMA, Yuichi KATO, Yasuo FUJIKAWA, Tomohiro TSURUMOTO
  • Patent number: 10351882
    Abstract: There is provided a method for generating an oil/fat component, in which salt-tolerant algae are cultured in a culture medium of which the salt concentration has been increased in a stepwise manner.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: July 16, 2019
    Assignees: National University Corporation KOBE University, Inter-University Research Institute Corporation National Institutes of Natural Sciences, DIC Corporation
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Shih-hsin Ho, Jun Minagawa, Haruo Nishie, Hiroyuki Taroda, Jo-Shu Chang
  • Patent number: 10214756
    Abstract: There is provided a method for generating an oil/fat component by means of culturing algae, in which marine algae belonging to chlamydomonas are cultured in a culture medium containing sea salt.
    Type: Grant
    Filed: March 17, 2014
    Date of Patent: February 26, 2019
    Assignees: National University Corporation KOBE University, Inter-University Research Institute Corporation National Institutes of Natural Sciences, DIC Corporation
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Shih-hsin Ho, Jun Minagawa, Haruo Nishie, Hiroyuki Taroda, Jo-Shu Chang
  • Patent number: 9751917
    Abstract: Disclosed is a polynucleotide for cell surface expression. The polynucleotide of the invention comprises a promoter, a secretion signal sequence, a sequence encoding an intended protein, and a sequence encoding a cell surface-localized protein or a cell membrane-binding domain thereof, wherein the promoter is a promoter of a gene encoding the cell surface-localized protein. Provided is a polynucleotide for cell surface expression allowing the production of yeast displaying an enzyme on the cell surface with a high activity.
    Type: Grant
    Filed: March 25, 2014
    Date of Patent: September 5, 2017
    Assignee: National University Corporation Kobe University
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Kentaro Inokuma
  • Publication number: 20170218382
    Abstract: An expression vector is disclosed which contains a promoter DNA; a DNA encoding a peptide having a defined amino acid sequence and having secretion signal activity; and a DNA encoding an intended protein or a cloning site for insertion of the DNA encoding an intended protein. An expression vector is also disclosed which contains a promoter DNA; a DNA encoding any peptide having a defined amino acid sequence and having secretion signal activity; a DNA encoding an intended protein or a cloning site for insertion of the DNA encoding an intended protein; and a DNA encoding an anchor domain. The peptide having secretion signal activity allows for secretory production and cell surface display of a protein with high activity, in yeast.
    Type: Application
    Filed: July 30, 2015
    Publication date: August 3, 2017
    Applicant: National University Corporation Kobe University
    Inventors: Akihiko KONDO, Tomohisa HASUNUMA, Kentaro INOKUMA
  • Publication number: 20160326224
    Abstract: Disclosed is a polynucleotide for cell surface expression. The polynucleotide of the invention comprises a promoter, a secretion signal sequence, a sequence encoding an intended protein, and a sequence encoding a cell surface-localized protein or a cell membrane-binding domain thereof, wherein the promoter is a promoter of a gene encoding the cell surface-localized protein. Provided is a polynucleotide for cell surface expression allowing the production of yeast displaying an enzyme on the cell surface with a high activity.
    Type: Application
    Filed: March 25, 2014
    Publication date: November 10, 2016
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Kentaro Inokuma
  • Patent number: 9441249
    Abstract: Provided is a method for efficiently producing ethanol by ethanol fermentation from xylose using a saccharified biomass, which contains various fermentation inhibitors. A method for producing ethanol from biomass of the present invention includes the step of culturing a xylose-utilizing yeast transformed so as to overexpress a gene for an acetic acid-responsive transcription factor in combination with a saccharified biomass.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: September 13, 2016
    Assignee: National University Corporation Kobe University
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Yuri Sakihama
  • Publication number: 20160215307
    Abstract: There is provided a method for generating an oil/fat component, in which salt-tolerant algae are cultured in a culture medium of which the salt concentration has been increased in a stepwise manner.
    Type: Application
    Filed: March 17, 2014
    Publication date: July 28, 2016
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Shih-hsin Ho, Jun Minagawa, Haruo Nishie, Hiroyuki Taroda, Jo-Shu Chang
  • Publication number: 20160201098
    Abstract: There is provided a method for generating an oil/fat component by means of culturing algae, in which marine algae belonging to Chlamydomonas are cultured in a culture medium containing sea salt.
    Type: Application
    Filed: March 17, 2014
    Publication date: July 14, 2016
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Shih-hsin Ho, Jun Minagawa, Haruo Nishie, Hiroyuki Taroda, Jo-Shu Chang
  • Publication number: 20160017379
    Abstract: An object of the present invention is to provide a method for producing ethanol efficiently even in the presence of a fermentation inhibitor in a saccharified biomass. The present invention provides a method for producing ethanol from biomass, comprising: culturing a transformed xylose-utilizing yeast to overexpress the gene for at least one pentose phosphate pathway metabolic enzyme, with a saccharified biomass.
    Type: Application
    Filed: October 2, 2015
    Publication date: January 21, 2016
    Applicant: NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Tomoya Sanda
  • Publication number: 20160011103
    Abstract: An optical measuring method for measuring a concentration of a fermentation inhibitor included in a biomass-derived fermentation raw material includes acquiring a diffuse reflection spectrum or a transmission spectrum relating to a measurement target 40 which includes the biomass-derived fermentation raw material by radiating near-infrared light to the measurement target 40, and computing the concentration of the fermentation inhibitor based on the diffuse reflection spectrum or the transmission spectrum.
    Type: Application
    Filed: July 7, 2015
    Publication date: January 14, 2016
    Inventors: Tetsu MORISHIMA, Akihiko KONDO, Tomohisa HASUNUMA, Akihito NAKANISHI
  • Publication number: 20150218592
    Abstract: Provided is a method for efficiently producing ethanol by ethanol fermentation from xylose using a saccharified biomass, which contains various fermentation inhibitors. A method for producing ethanol from biomass of the present invention includes the step of culturing a xylose-utilizing yeast transformed so as to overexpress a gene for an acetic acid -responsive transcription factor in combination with a saccharified biomass.
    Type: Application
    Filed: August 23, 2013
    Publication date: August 6, 2015
    Applicant: National University Corporation Kobe University
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Yuri Sakihama
  • Publication number: 20140120598
    Abstract: Provided is a novel method of producing ethanol by using a cellulose-based biomass as a raw material. In particular, provided is a novel method of producing ethanol by which ethanol can be effectively produced in the presence of a substance having an inhibitory action on fermentation of ethanol. Ethanol can be effectively produced by using a microorganism engineered to suppress the expression of at least one kind of phosphatase among the phosphatases intrinsically possessed by the microorganism, even under a condition where a substance that has heretofore been believed to have a fermentation inhibitory action, specifically, a weakly acidic substance and/or a furan compound are/is incorporated.
    Type: Application
    Filed: June 25, 2012
    Publication date: May 1, 2014
    Applicant: National University Corporation KOBE University
    Inventors: Akihiko Kondo, Tomohisa Hasunuma
  • Publication number: 20120282664
    Abstract: An object of the present invention is to provide a method for producing ethanol efficiently even in the presence of a fermentation inhibitor in a saccharified biomass. The present invention provides a method for producing ethanol from biomass, comprising: culturing a transformed xylose-utilizing yeast to overexpress the gene for at least one pentose phosphate pathway metabolic enzyme, with a saccharified biomass.
    Type: Application
    Filed: November 29, 2010
    Publication date: November 8, 2012
    Inventors: Akihiko Kondo, Tomohisa Hasunuma, Tomoya Sanda