Patents by Inventor Tomoji Kawai

Tomoji Kawai has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10876159
    Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.
    Type: Grant
    Filed: December 28, 2018
    Date of Patent: December 29, 2020
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
  • Patent number: 10877021
    Abstract: A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.
    Type: Grant
    Filed: April 21, 2017
    Date of Patent: December 29, 2020
    Assignees: OSAKA UNIVERSITY, TOKYO INSTITUTE OF TECHNOLOGY, NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY
    Inventors: Makusu Tsutsui, Kazumichi Yokota, Masateru Taniguchi, Tomoji Kawai, Mina Okochi, Masayoshi Tanaka, Yoshinobu Baba, Noritada Kaji, Takao Yasui, Yuji Miyahara, Yukichi Horiguchi
  • Patent number: 10830685
    Abstract: Provided is a device for electrical measurement designed to be able to perform high sensitivity detection by reading not only changes in steady-state current, but also the occurrence of transient current, and an electrical measurement apparatus including the device for electrical measurement. The device for electrical measurement includes a substrate on which are formed at least a sample separation channel and a sample migration channel, as well as a sample measuring unit, with one end of the sample separation channel formed to connect to one end of the sample migration channel, and the sample measuring unit including a first measuring unit connected to the sample migration channel, and a second measuring unit connected to the sample migration channel from the reverse side to the first measuring unit.
    Type: Grant
    Filed: April 6, 2016
    Date of Patent: November 10, 2020
    Assignee: NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITY
    Inventors: Yoshinobu Baba, Noritada Kaji, Takao Yasui, Tomoji Kawai, Takeshi Yanagida
  • Publication number: 20200251184
    Abstract: The present invention provides a classification analysis method, a classification analysis device, and a storage medium for classification analysis, which enable, with high accuracy, the classification analysis of particulate or molecular analytes. As a means for solving the problem, a data group of particle-passage detection signals is based which are detected by a nanopore device 8 in accordance with passage of subject particles through a through-hole 12. A feature value is obtained in advance which indicates the feature of the waveform of the pulse signals corresponding to the passage of the predetermined analyte and the feature value obtained in advance is set as the learning data for the machine learning. The feature value obtained from the pulse signals of said analyzed data is set as a variable and the classification analysis on the predetermined analytes in the analyzed data can be performed by executing a classification analysis program due to the machine learning.
    Type: Application
    Filed: December 12, 2017
    Publication date: August 6, 2020
    Applicant: Osaka University
    Inventors: Takashi WASHIO, Tomoji KAWAI, Masateru TANIGUCHI, Makusu TSUTSUI, Kazumichi YOKOTA, Akira ISHI, Takeshi YOSHIDA
  • Publication number: 20200070169
    Abstract: Provided is a channel device that is capable of increasing the concentration of fine particles in a liquid only by use of fluid-dynamic flows without relying on electrostatic interactions. A channel device (1) in accordance with an embodiment of the present invention includes: a main channel (11) configured to allow a liquid containing fine particles to flow therethrough; a chamber (15) that is provided at an end of the main channel (11) and that is configured to store target fine particles which have increased in concentration; and a side channel (12) that is connected to a side face of the main channel (11) and that is configured to allow unwanted liquid to drain therethrough, wherein at least one of a height and a width of the side channel (12) is smaller than a particle size of the fine particles.
    Type: Application
    Filed: May 9, 2018
    Publication date: March 5, 2020
    Inventors: Wataru TONOMURA, Makusu TSUTSUI, Kazumichi YOKOTA, Akihide ARIMA, Masateru TANIGUCHI, Tomoji KAWAI
  • Patent number: 10557167
    Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.
    Type: Grant
    Filed: March 2, 2017
    Date of Patent: February 11, 2020
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro, Mark Oldham, Eric Nordman
  • Publication number: 20200033248
    Abstract: In a method for analyzing samples involving the use of a device for analyzing samples, the device for analyzing samples includes at least a movement part through which a sample moves, and a measurement unit that is formed in a middle of the movement part and that measures a value of an ion current when the sample passes through the movement part. The analysis method includes at least a measurement step for measuring the value of the ion current when the sample passes through the movement part, and a determination step for determining a change over time in a quantity of ions from the value of the ion current measured in the measurement step. The quantity of ions includes a quantity of ions that have leaked from the sample during movement of the sample through the movement part.
    Type: Application
    Filed: October 4, 2017
    Publication date: January 30, 2020
    Inventors: Hirotoshi YASAKI, Takao YASUI, Noritada KAJI, Yoshinobu BABA, Tomoji KAWAI, Satoyuki KAWANO, Kentaro DOI, Takeshi YANAGIDA, Mao FUKUYAMA
  • Publication number: 20190367979
    Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.
    Type: Application
    Filed: December 28, 2018
    Publication date: December 5, 2019
    Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
  • Publication number: 20190257787
    Abstract: The present invention provides a number analyzing method, a number analyzing device, and a storage medium for number analysis, which enable, with high accuracy, analysis of the number or number distribution of particulate or molecular analytes according to the kinds of the analytes. A computer control program is executed on the basis of a data group of particle-passage detection signals which are detected by a nanopore device (8) in accordance with passage of subject particles through a through-hole (12). Also, a particle type distribution estimating program, which is a number deriving means, is executed, to estimate probability density on the basis of a data group based on feature values indicating feature of the waveforms of pulse signals which correspond to the passage of particles and which are obtained as the particle-passage detection signals. Thus, the number of particles can be derived for each particle type.
    Type: Application
    Filed: December 19, 2016
    Publication date: August 22, 2019
    Applicant: Osaka University
    Inventors: Takashi WASHIO, Tomoji KAWAI, Masateru TANIGUCHI, Makusu TSUTSUI, Kazumichi YOKOTA, Akira ISHI, Takeshi YOSHIDA
  • Publication number: 20190128888
    Abstract: A device for biological material detection includes a substrate; a through-hole through which a biological material to be tested passes, the through-hole being formed in the substrate; a molecule that interacts with the biological material to be tested passing through, the molecule being formed in the through-hole; a first chamber member that forms, with at least the surface including the through-hole on one surface side of the substrate, a first chamber to be filled with electrolyte; and a second chamber member that forms, with at least the surface including the through-hole on the other surface side of the substrate, a second chamber to be filled with electrolyte. The biological material to be tested is identified by the waveform of the ion current (passage time, shape, etc.) when the biological material to be tested passes through the through-hole.
    Type: Application
    Filed: April 21, 2017
    Publication date: May 2, 2019
    Inventors: Makusu TSUTSUI, Kazumichi YOKOTA, Masateru TANIGUCHI, Tomoji KAWAI, Mina OKOCHI, Masayoshi TANAKA, Yoshinobu BABA, Noritada KAJI, Takao YASUI, Yuji MIYAHARA, Yukichi HORIGUCHI
  • Patent number: 10202644
    Abstract: The present invention provides technology that uses current measurements to identify nucleotides and determine a nucleotide sequence in polynucleotides. The present invention calculates a modal value of a tunnel current that arises when a nucleotide or polynucleotide for analysis passes through between electrodes, and then employs the calculated modal value. The present invention accordingly enables direct rapid implementation to identify nucleotides and to determine a nucleotide sequence in a polynucleotide without marking.
    Type: Grant
    Filed: October 14, 2015
    Date of Patent: February 12, 2019
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Masateru Taniguchi, Makusu Tsutsui, Kazumichi Yokota, Tomoji Kawai
  • Publication number: 20180100792
    Abstract: Provided is a device for electrical measurement designed to be able to perform high sensitivity detection by reading not only changes in steady-state current, but also the occurrence of transient current, and an electrical measurement apparatus including the device for electrical measurement. The device for electrical measurement includes a substrate on which are formed at least a sample separation channel and a sample migration channel, as well as a sample measuring unit, with one end of the sample separation channel formed to connect to one end of the sample migration channel, and the sample measuring unit including a first measuring unit connected to the sample migration channel, and a second measuring unit connected to the sample migration channel from the reverse side to the first measuring unit.
    Type: Application
    Filed: April 6, 2016
    Publication date: April 12, 2018
    Inventors: Yoshinobu BABA, Noritada KAJI, Takao YASUI, Tomoji KAWAI, Takeshi YANAGIDA
  • Publication number: 20180023132
    Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.
    Type: Application
    Filed: March 2, 2017
    Publication date: January 25, 2018
    Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro, Mark Oldham, Eric Nordman
  • Publication number: 20170306396
    Abstract: The nano-gap electrode pair 12 is disposed so that a biomolecule joined to at least one or more types of a single molecule included in a sample passes an opposing position, and the strength of the electric field in a position spaced only a predetermined distance on the downstream side from the opposing position 64 becomes stronger than the strength of the electric field in a position spaced only the predetermined distance on the upstream side from the opposing position 64.
    Type: Application
    Filed: August 19, 2016
    Publication date: October 26, 2017
    Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro
  • Patent number: 9726636
    Abstract: According to one embodiment, provided is a single particle analyzing device including a measuring vessel, first and second chambers in the vessel defined by an insulating membrane, a pore opening in the membrane to connect the chambers, and first and second electrodes in the chambers. Electric current flows between the electrodes through the pore. Electrical characteristics are measured during migration of the target from the first chamber to the second chamber to measure the size and shape of the target. (a) t<a <d?100a or (b) s<L, s<d?100s, t<L and t<d, wherein a, L and s are the diameter, length and width of the target, d is the diameter of the pore, and t is the thickness of the membrane in the proximity to the pore.
    Type: Grant
    Filed: September 12, 2014
    Date of Patent: August 8, 2017
    Assignees: KABUSHIKI KAISHA TOSHIBA, OSAKA UNIVERSITY
    Inventors: Sadato Hongo, Tomoji Kawai, Makusu Tsutsui, Masateru Taniguchi, Soh Ryuzaki
  • Publication number: 20170146511
    Abstract: The present disclosure provides devices, systems and methods for effectuating nanoelectrodes for use with determining the sequence of double stranded biopolymers. Various modified bases and different metals may be utilized alone or in combination so as to provide differentiation between different nucleobases and to determine which base is associated with which strand.
    Type: Application
    Filed: November 4, 2016
    Publication date: May 25, 2017
    Inventors: Masateru Taniguchi, Tomoji Kawai, Takahito Ohshiro
  • Patent number: 9644236
    Abstract: Devices, systems and methods for sequencing protein samples are provided. In some examples, currents generated when a monomer passes through between electrodes of a nanogap electrode pair are measured for each of several different distances, so that monomers are identified when compared to a reference physical quantity of a known monomer, which may be obtained from a current measured with a similar inter-electrode distance(s) at which each of plural kinds of monomers are identifiable and ordered with predetermined accuracy and based on a detected physical quantity obtained from a tunneling current, which may be further normalized by the use of one or more reference substances.
    Type: Grant
    Filed: March 4, 2016
    Date of Patent: May 9, 2017
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro, Mark Oldham, Eric Nordman
  • Patent number: 9535033
    Abstract: The analysis method allows analysis of samples with high sensitivity, irrespective of interelectrode distance. The method includes: a step of applying a voltage between a first electrode pair such that an electric field is formed in a direction intersecting a migration direction of a sample; a step of placing a solution, including an electrochemically active molecule that produces a redox reaction at the electrode pair, between the first electrode pair; a step of causing the sample to migrate; and a step of measuring an amount of change in current flow between the first electrode pair.
    Type: Grant
    Filed: August 2, 2013
    Date of Patent: January 3, 2017
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Tomoji Kawai, Takahito Ohshiro, Masateru Taniguchi
  • Publication number: 20160377591
    Abstract: The present disclosure provides a biomolecule sequencing device that includes at least one set of nano-gap electrodes arranged so that a current flows when a biomolecule contained in a sample passes in proximity to the set of nano-gap electrodes, an electrophoresis electrode pair for forming an electric field for moving the biomolecule between the electrodes of the set of nano-gap electrodes, a flow path for flowing the sample in a direction towards the nano-gap electrode pair, a flow path for flowing the sample in a direction away from the nano-gap electrode pair, a measurement unit configured to measure a tunnel current generated when the biomolecule passes between the electrodes of the nano-gap electrode pair with an electric field being formed, and an identification unit configured to sequence the biomolecule.
    Type: Application
    Filed: July 22, 2016
    Publication date: December 29, 2016
    Inventors: Tomoji Kawai, Masateru Taniguchi, Takahito Ohshiro, Mark Floyd Oldham, Eric Scott Nordman
  • Patent number: 9506894
    Abstract: The present invention provides a method and apparatus for controlling the moving speed of a substance, both of which can adjust the moving speed of a substance to a desired speed. The control method and control apparatus cause a substance to pass through an internal space, in which an electro-osmotic flow is generated, of a surround electrode formed so as to surround part of the moving path of the substance, whereby the control method and control apparatus change the moving speed of the substance.
    Type: Grant
    Filed: August 26, 2013
    Date of Patent: November 29, 2016
    Assignee: QUANTUM BIOSYSTEMS INC.
    Inventors: Tomoji Kawai, Soh Ryuzaki, Masateru Taniguchi