Abstract: The present invention addresses the problem of providing a method for observing skin tissue in which x-ray microCT is used. Adnexae such as sweat glands, sebaceous glands, and hair follicles can be identified in skin tissue by using acetone as a pretreatment solution and staining using an iodine-containing solution as a staining solution.

Abstract: This invention provides a method of causing proliferation of stem cells and their derivative cells and thus solves the cosmetic problems of wrinkles and sagging. Mechanical stress is applied to skin that includes sebaceous glands, thereby causing proliferation of stem cells and their derivative cells.

Abstract: The purpose of the present invention is to provide an agent for improving skin looseness or aging caused by dermal cavitation. By attracting fat stem cells into cavities in the dermis, dermal fibroblasts around the cavities can be activated and thus dermal cavitation can be ameliorated. As the results of a screening for fat stem cell attractants, it was found that rosemary extract and licorice extract are capable of attracting fat stem cells and, therefore, can improve skin looseness or aging caused by dermal cavitation.

Abstract: The purpose of the invention is to develop a method for screening anti-aging substances. The present inventors have found a phenomenon in which a dermis aging factor secreted by senescent cells acts on normal cells to promote the decomposition of dermal components and a reduction in the production of dermal components, and have arrived at developing a method for screening anti-aging substances by employing the dermis aging factor as an indicator.

Abstract: [PROBLEM] To provide a mesenchymal stem cell attracting agent, as well as an aging-factor production inhibiting agent, anti-aging agent, and dermal regeneration promoting agent including the attracting agent. [SOLVING MEANS] It was found that iris extract, olive leaf extract, and chamomile extract have a mesenchymal stem cell inducing action, which led to the invention of a mesenchymal stem cell attracting agent. Furthermore, it was discovered that mesenchymal stem cells inhibit aging factor production and activate dermal fibroblasts, whereby an aging-factor production inhibiting agent, anti-aging agent, and dermal regeneration promoting agent including the attracting agent were invented.

Abstract: The present invention addresses the problem of providing a method for observing skin tissue in which x-ray microCT is used. Adnexae such as sweat glands, sebaceous glands, and hair follicles can be identified in skin tissue by using acetone as a pretreatment solution and staining using an iodine-containing solution as a staining solution.

Abstract: The purpose of the present invention is to provide an agent for improving skin looseness or aging caused by dermal cavitation. By attracting fat stem cells into cavities in the dermis, dermal fibroblasts around the cavities can be activated and thus dermal cavitation can be ameliorated. As the results of a screening for fat stem cell attractants, it was found that rosemary extract and licorice extract are capable of attracting fat stem cells and, therefore, can improve skin looseness or aging caused by dermal cavitation.

Abstract: The present invention is directed to method for preventing and improving an aggravation of a skin condition accompanied with a skin elastic property loss by suppressing an increase in, or inhibiting the physiological activity of, palmitic acid. The present invention provides a cosmetic method for preventing and improving wrinkling and sagging accompanied with a skin elastic property loss and a method for preventing and treating a wound healing disorder and a bedsore.

Abstract: A cosmetic method for preventing an aggravation of skin condition accompanied with elasticity loss such as sag and a wrinkle is developed. The invention provides the method for preventing an elastic property loss specifically including the step of suppressing the increase in subcutaneous fat. In the step of the present invention for preventing the elastic property loss, the step of suppressing the increase in the subcutaneous fat may include a step of a thermal stimulation. In the step of the present invention for preventing the elastic property loss, the step may include a step of administering to a subject a composition which suppresses an increase in subcutaneous fat. The present invention provides a cosmetic method for preventing an aggravation of skin condition accompanied with elasticity loss, specifically including a step of applying to a skin a method for preventing the elastic property loss of skin.

Abstract: It is an object of the invention to develop a method and an apparatus for improving a skin condition by improving the condition of the subcutaneous fat tissue that induces abnormal secretion of adipocytokines by the subcutaneous fat cells. The present invention provides a method for improving skin condition of the face and/or neck. The method for improving skin condition of the present invention comprises a step for applying 60 to 90 minutes of thermal stimulation at 41 to 43° C. to the subcutaneous fat of the face and/or neck. The present invention also provides an apparatus for improving the skin condition of the face and/or neck. The apparatus for improving skin condition of the present invention comprises a heater and applies 60 to 90 minutes of thermal stimulation at 41 to 43° C. to the subcutaneous fat of the face and/or neck.