Abstract: A cell culture apparatus is provided, including a storage tank including one or a plurality of cell culture units, in which the cell culture unit includes a culture chamber having an inner surface-side space in which a culture solution is stored, a permeable membrane having a first surface to which cells are adherable and a second surface opposite to the first surface, the first surface facing the inner surface-side space, a culture solution storage chamber that stores the culture solution, a culture solution introduction flow path that introduces the culture solution in the culture solution storage chamber to the inner surface-side space, and a culture solution discharge flow path that sends, to the culture solution storage chamber, the culture solution which permeates through the membrane from the inner surface-side space and flows into an outer surface-side space that the second surface of the membrane faces.

Abstract: A polymer compound is provided which is changed from a water-insoluble state to a water-soluble state by irradiation with light. The polymer compound is represented by Formula (5), where A and B are a single bond or a functional group, R3, R4, and R9 are hydrogen or an alkyl group, and R6 and R7 are hydrogen, an alkyl group, or the like.

Abstract: A cell culture instrument configured to detach cells at a desired position and a cell processing method using the cell culture instrument. The cell culture instrument includes: a substrate; and a photoreactive layer having a photosolubility and a photothermal convertibility, wherein the photoreactive layer is laminated on the substrate, and the photoreactive layer includes a polymer having a photosolubility and a photothermal convertibility.

Abstract: A cell culture apparatus, includes a connected culture container including n number of units disposed in parallel along a second direction that is a different direction from a first direction where each of the n number of the units is constituted of m number of culture chambers and one or more communication-channels, the m number of the culture chambers each having a cell-holding portion that holds seeded cells, the m number of the culture chambers storing liquid culture media, the m number of the culture chambers being disposed in parallel along the first direction, the communication-channels communicating the m number of the culture chambers with each other; and a plurality of pneumatic pipes communicating same-row-disposed culture chambers that are disposed on a same row along the second direction with each other in the connected culture container.

Abstract: A cell culture device includes a storage tank having one or a plurality of cell culture units. The cell culture units includes a culture liquid main chamber having a circulation space through which a culture liquid of cells circulates, a permeable membrane having one surface to which the cells are able to adhere, said one face facing the circulation space, a first culture liquid storage chamber having an airtight structure in which the culture liquid is to be stored, a second culture liquid storage chamber in which the culture liquid is to be stored, a culture liquid introduction flow path that introduces the culture liquid from the first culture liquid storage chamber into the circulation space of the culture liquid main chamber, and a culture liquid lead-out flow path that introduces the culture liquid from the circulation space into the second culture liquid storage chamber.

Abstract: A cell culture device includes a storage tank having one or a plurality of cell culture units. Each of the cell culture units includes a first liquid storage chamber having an airtight structure in which a liquid is to be stored, a second liquid storage chamber in which the liquid is to be stored, a culture liquid storage chamber having a culture liquid storage space in which a culture liquid of cells is to be stored, a permeable membrane having one surface to which the cells are able to adhere, said one face facing the culture liquid storage space, and a liquid lead-out flow path that introducing the liquid from a space on the other surface side of the membrane into the second liquid storage chamber, the first liquid storage chamber being set as a supply source of the liquid.

Abstract: A laser processing machine for killing specific cells from a group of cells on a surface of a layer containing an ingredient capable of absorbing laser light, the laser processing machine being configured to: control a laser light source to output laser light at 5 W or less and at a wavelength of 380 nm or greater such that the laser light source is applied to a second area on a second surface of the layer opposed to the first surface; and control an actuator to provide a relative movement between the second area where the laser light is applied and the layer at a rate of 2000 mm/sec or less such that the irradiated second area absorbs energy to generate heat that kills unwanted cells on a first area of the first surface and the laser light does not instantly kill the specific cells on the first area upon irradiation.

Abstract: A cell culture vessel for use with a laser processing machine, including: a layer containing an ingredient that generates heat upon laser irradiation; the layer kills specific cells from among a group of cells cultured on a first surface of the layer when a second surface of the layer is irradiated with laser light having an output of 5W or less at a wavelength of 380 nm or greater and a relative movement between a second area on the second surface where the laser light is applied and the layer is at a rate of 2000 mm/sec or less such that the second area absorbs energy of the laser light to generate heat that kills the specific cells that are present on a first area of the first surface and the laser light does not instantly kill the specific cells on the first area upon irradiation with the laser light.

Abstract: A method for producing a chip for cell culture that enables production of a microchannel structure, enables mass production at low cost, and also has functions suited to cell culture. The method includes forming a photosensitive adhesive layer by applying a photosensitive adhesive to a first substrate having transparency, thus obtaining a 3D printing substrate, 3D printing steps that employ vat photopolymerization, forming a first adhesive layer by depositing a first adhesive on a second substrate having transparency, thus obtaining a cell culture channel top plate, and bonding the cell culture channel and the cell culture channel top plate together via the first adhesive layer, and then performing thermocompression bonding to obtain a microchannel structural body having a hollow structure, wherein the first adhesive is a polyester-based resin having a Tg value of 5° C. or higher.

Abstract: A method of killing specific cells from among a group of cells cultured in a culture vessel by quick and brief laser treatment, the cell culture vessel comprising a main body and a to-be-irradiated layer attached to the main body, the to-be-irradiated layer containing an ingredient capable of absorbing laser light upon laser irradiation, the group of cells being cultured on the surface of the to-be-irradiated layer, the method comprising: applying laser light to a partial area of the to-be-irradiated layer directly below the specific cells.

Abstract: A polymer compound is provided which is changed from a water-insoluble state to a water-soluble state by irradiation with light. The polymer compound is represented by Formula (5), where A and B are a single bond or a functional group, R3, R4, and R9 are hydrogen or an alkyl group, and R6 and R7 are hydrogen, an alkyl group, or the like.

Abstract: Provided are a photodegradable hydrogel in which cells can be embedded in the photodegradable gel without causing cytotoxicity when the cells are embedded in the photodegradable gel by allowing the cells to coexist at the time of preparation of the photodegradable gel, and which contains a protein as one of the main components; a culture device using the same; a method for forming tissue; and a method for separating cells. A photodegradable hydrogel is obtained by condensation of an alkyne group contained in a cyclooctyne ring or an azacyclooctyne ring of the following compound A with an azido group of the following compound B.

Abstract: A cell culture apparatus is provided, including a storage tank including one or a plurality of cell culture units, in which the cell culture unit includes a culture chamber having an inner surface-side space in which a culture solution is stored, a permeable membrane having a first surface to which cells are adherable and a second surface opposite to the first surface, the first surface facing the inner surface-side space, a culture solution storage chamber that stores the culture solution, a culture solution introduction flow path that introduces the culture solution in the culture solution storage chamber to the inner surface-side space, and a culture solution discharge flow path that sends, to the culture solution storage chamber, the culture solution which permeates through the membrane from the inner surface-side space and flows into an outer surface-side space that the second surface of the membrane faces.

Abstract: A cell culture device includes a storage tank having one or a plurality of cell culture units. The cell culture units includes a culture liquid main chamber having a circulation space through which a culture liquid of cells circulates, a permeable membrane having one surface to which the cells are able to adhere, said one face facing the circulation space, a first culture liquid storage chamber having an airtight structure in which the culture liquid is to be stored, a second culture liquid storage chamber in which the culture liquid is to be stored, a culture liquid introduction flow path that introduces the culture liquid from the first culture liquid storage chamber into the circulation space of the culture liquid main chamber, and a culture liquid lead-out flow path that introduces the culture liquid from the circulation space into the second culture liquid storage chamber.

Abstract: A cell culture device includes a storage tank having one or a plurality of cell culture units. Each of the cell culture units includes a first liquid storage chamber having an airtight structure in which a liquid is to be stored, a second liquid storage chamber in which the liquid is to be stored, a culture liquid storage chamber having a culture liquid storage space in which a culture liquid of cells is to be stored, a permeable membrane having one surface to which the cells are able to adhere, said one face facing the culture liquid storage space, and a liquid lead-out flow path that introducing the liquid from a space on the other surface side of the membrane into the second liquid storage chamber, the first liquid storage chamber being set as a supply source of the liquid.

Abstract: A method for producing a chip for cell culture that enables production of a microchannel structure, enables mass production at low cost, and also has functions suited to cell culture. The method includes forming a photosensitive adhesive layer by applying a photosensitive adhesive to a first substrate having transparency, thus obtaining a 3D printing substrate, 3D printing steps that employ vat photopolymerization, forming a first adhesive layer by depositing a first adhesive on a second substrate having transparency, thus obtaining a cell culture channel top plate, and bonding the cell culture channel and the cell culture channel top plate together via the first adhesive layer, and then performing thermocompression bonding to obtain a microchannel structural body having a hollow structure, wherein the first adhesive is a polyester-based resin having a Tg value of 5° C. or higher.

Abstract: A method of killing specific cells from among a group of cells cultured in a culture vessel by quick and brief laser treatment, the cell culture vessel comprising a main body and a to-be-irradiated layer attached to the main body, the to-be-irradiated layer containing an ingredient capable of absorbing laser light upon laser irradiation, the group of cells being cultured on the surface of the to-be-irradiated layer, the method comprising: applying laser light to a partial area of the to-be-irradiated layer directly below the specific cells.

Abstract: A method for producing a structure composed of crosslinked polymer, including laminating a photo-dissoluble polymer layer on a substrate; laminating a layer containing a crosslinkable polymer on the photo-dissoluble polymer layer; irradiating the layer containing the crosslinkable polymer with light in a patternwise manner under crosslinking conditions to crosslink the crosslinkable polymer in a patternwise manner to obtain a crosslinked polymer sheet; washing and removing a uncrosslinked crosslinkable polymer to obtain a patterned crosslinked polymer sheet; and irradiating the photo-dissoluble polymer layer with light in a patternwise manner under dissolution conditions to dissolve the photo-dissoluble polymer layer in a patternwise manner, and peeling the crosslinked polymer sheet in a patternwise manner from the substrate.

Abstract: A cell culture apparatus, includes a connected culture container including n number of units disposed in parallel along a second direction that is a different direction from a first direction where each of the n number of the units is constituted of m number of culture chambers and one or more communication-channels, the m number of the culture chambers each having a cell-holding portion that holds seeded cells, the m number of the culture chambers storing liquid culture media, the m number of the culture chambers being disposed in parallel along the first direction, the communication-channels communicating the m number of the culture chambers with each other; and a plurality of pneumatic pipes communicating same-row-disposed culture chambers that are disposed on a same row along the second direction with each other in the connected culture container.

Abstract: Provided are a photodegradable hydrogel in which cells can be embedded in the photodegradable gel without causing cytotoxicity when the cells are embedded in the photodegradable gel by allowing the cells to coexist at the time of preparation of the photodegradable gel, and which contains a protein as one of the main components; a culture device using the same; a method for forming tissue; and a method for separating cells. A photodegradable hydrogel is obtained by condensation of an alkyne group contained in a cyclooctyne ring or an azacyclooctyne ring of the following compound A with an azido group of the following compound B.