Patents by Inventor Trevor Lane Broadt

Trevor Lane Broadt has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10954492
    Abstract: Described are improved processes for production and purification of nucleic acid-containing compositions, such as non-naturally occurring viruses, for example, recombinant polioviruses that can be employed as oncolytic agents. Some of the described improved processes relate to improved processes for producing viral DNA template. Also described are improved processes for chromatography purification of nucleic acid-containing compositions, in which the nucleic acid is quantified in chromatography fractions using a rapid detection method of the one or more nucleic acid sequences of the nucleic acid-containing composition, such as detection by real time RT-qPCR. In addition, improved processes for production and purification of oncolytic poliovirus, such as PVS-RIPO, are described. Compositions generated using these methods are also provided.
    Type: Grant
    Filed: June 10, 2016
    Date of Patent: March 23, 2021
    Assignee: The United States of America, as represented by the Secretary, Department of Health and Human Services
    Inventors: Trevor Lane Broadt, Samir Hussein Shaban, Yueqing Xie, Jianwei Sean Zhu, George Mitra
  • Patent number: 10662464
    Abstract: Described herein are massively parallel sequencing methods for virus-derived therapeutics such as viral vaccines, including the PVS-RIPO vaccine. The methods allow for the determination of micro-heterogeneity and quantitation of low frequency sequence variants and in the case of PVS-RIPO, are expected to replace the monkey neurovirulence safety test (MNVT) and the mutant analysis by PCR and restriction enzyme cleavage (MAPREC) methods that are currently used to screen lots of RNA virus-derived therapeutics.
    Type: Grant
    Filed: July 29, 2016
    Date of Patent: May 26, 2020
    Assignees: The United States of America, as represented by the Secretary, Department of Health and Human Serv, American International Biotechnology, LLC
    Inventors: Trevor Lane Broadt, Michael D. Harwich, William T. Budd, Gregory A. Meyers
  • Publication number: 20180237835
    Abstract: Described herein are massively parallel sequencing methods for virus-derived therapeutics such as viral vaccines, including the PVS-RIPO vaccine. The methods allow for the determination of micro-heterogeneity and quantitation of low frequency sequence variants and in the case of PVS-RIPO, are expected to replace the monkey neurovirulence safety test (MNVT) and the mutant analysis by PCR and restriction enzyme cleavage (MAPREC) methods that are currently used to screen lots of RNA virus-derived therapeutics.
    Type: Application
    Filed: July 29, 2016
    Publication date: August 23, 2018
    Applicants: The United States of America, as represented by the Secretary, Department of Health and Human Serv, American International Biotechnology, LLC
    Inventors: Trevor Lane Broadt, Michael D. Harwich, William T. Budd, Gregory A. Meyers
  • Publication number: 20180163182
    Abstract: Described are improved processes for production and purification of nucleic acid-containing compositions, such as non-naturally occurring viruses, for example, recombinant polioviruses that can be employed as oncolytic agents. Some of the described improved processes relate to improved processes for producing viral DNA template. Also described are improved processes for chromatography purification of nucleic acid-containing compositions, in which the nucleic acid is quantified in chromatography fractions using a rapid detection method of the one or more nucleic acid sequences of the nucleic acid-containing composition, such as detection by real time RT-qPCR. In addition, improved processes for production and purification of oncolytic poliovirus, such as PVS-RIPO, are described. Compositions generated using these methods are also provided.
    Type: Application
    Filed: June 10, 2016
    Publication date: June 14, 2018
    Applicants: Human Services
    Inventors: Trevor Lane Broadt, Samir Hussein Shaban, Yueqing Xie, Jianwei Sean Zhu, George Mitra