Abstract: A detection device adapted for detecting an analyte in a sample includes a substrate having a top surface, a sample pad, a binding pad, a cellulose pad, a nitrocellulose membrane and an absorbent pad. The sample pad is for receiving the sample. The binding pad includes a main body and a first detecting reagent disposed on the main body and is adapted for specifically binding to the analyte. The cellulose pad has a first connecting end portion and a second connecting end portion. The nitrocellulose membrane includes a membrane body and a detection zone that includes a second detecting reagent adapted for specifically binding to the analyte. The absorbent pad connects to the membrane body.