Patents by Inventor Vecheslav Elagin

Vecheslav Elagin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9856514
    Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.
    Type: Grant
    Filed: November 18, 2014
    Date of Patent: January 2, 2018
    Assignee: Meridian Bioscience, Inc.
    Inventors: Vladimir I Slepnev, Ahmer Kodvawala, Brian Loeffler, Anand Hindupur, Reddy Ponaka, Vecheslav A. Elagin
  • Publication number: 20170247680
    Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.
    Type: Application
    Filed: April 4, 2017
    Publication date: August 31, 2017
    Inventors: Vladimir I. Slepnev, Ahmer Kodvawala, Brian Loeffler, Anand Hindupur, Reddy Ponaka, Vecheslav A. Elagin
  • Patent number: 9249455
    Abstract: Disclosed are methods, kits, and components for detecting small RNA molecules, such as microRNA and siRNA, in a sample. The methods utilize primers and reporter molecules comprising non-natural bases. The disclosed kits may include one or more components for performing the disclosed methods.
    Type: Grant
    Filed: April 16, 2009
    Date of Patent: February 2, 2016
    Assignee: LUMINEX CORPORATION
    Inventors: Michael J. Moser, Vecheslav A. Elagin
  • Publication number: 20150079601
    Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.
    Type: Application
    Filed: November 18, 2014
    Publication date: March 19, 2015
    Inventors: Vladimir I. Slepnev, Ahmer Kodvawala, Brian Loeffler, Anand Hindupur, Reddy Ponaka, Vecheslav A. Elagin
  • Patent number: 8765418
    Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.
    Type: Grant
    Filed: February 15, 2013
    Date of Patent: July 1, 2014
    Assignee: Third Wave Technologies, Inc.
    Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
  • Publication number: 20130149697
    Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.
    Type: Application
    Filed: February 15, 2013
    Publication date: June 13, 2013
    Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, JR., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
  • Patent number: 8389245
    Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.
    Type: Grant
    Filed: September 19, 2007
    Date of Patent: March 5, 2013
    Assignee: Third Wave Technologies, Inc.
    Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Jr., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
  • Patent number: 8354232
    Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: January 15, 2013
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor I. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
  • Patent number: 8071750
    Abstract: The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.
    Type: Grant
    Filed: December 30, 2008
    Date of Patent: December 6, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: Vecheslav A. Elagin, Scott M. Law, Bjork Hill
  • Publication number: 20100285488
    Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.
    Type: Application
    Filed: July 20, 2010
    Publication date: November 11, 2010
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor A. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
  • Patent number: 7759062
    Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.
    Type: Grant
    Filed: June 11, 2007
    Date of Patent: July 20, 2010
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor A. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
  • Publication number: 20090275039
    Abstract: Disclosed are methods, kits, and components for detecting small RNA molecules, such as microRNA and siRNA, in a sample. The methods utilize primers and reporter molecules comprising non-natural bases. The disclosed kits may include one or more components for performing the disclosed methods.
    Type: Application
    Filed: April 16, 2009
    Publication date: November 5, 2009
    Inventors: Michael J. Moser, Vecheslav A. Elagin
  • Patent number: 7527948
    Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.
    Type: Grant
    Filed: September 27, 2004
    Date of Patent: May 5, 2009
    Assignee: Third Wave Technologies, Inc.
    Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Jr., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
  • Publication number: 20090111092
    Abstract: The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.
    Type: Application
    Filed: December 30, 2008
    Publication date: April 30, 2009
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Vecheslav A. Elagin, Scott M. Law, Bjork Hill
  • Patent number: 7473773
    Abstract: The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.
    Type: Grant
    Filed: January 7, 2005
    Date of Patent: January 6, 2009
    Assignee: Third Wave Technologies, Inc.
    Inventors: Vecheslav A. Elagin, Scott M. Law, Bjork Hill
  • Publication number: 20080176215
    Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.
    Type: Application
    Filed: September 19, 2007
    Publication date: July 24, 2008
    Applicant: Third Wave Technologies, Inc.
    Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
  • Publication number: 20080131870
    Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.
    Type: Application
    Filed: June 11, 2007
    Publication date: June 5, 2008
    Applicant: Third Wave Technologies, Inc.
    Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor A. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
  • Publication number: 20070207455
    Abstract: The present invention provides methods and compositions for detecting hepatitis C virus (HCV). In particular, the present invention provides nucleic acid detection assays configured to detect a novel sub-type of HCV-1.
    Type: Application
    Filed: November 17, 2006
    Publication date: September 6, 2007
    Applicant: Third Wave Technologies, Inc.
    Inventors: Scott Law, Vecheslav Elagin
  • Publication number: 20070111200
    Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.
    Type: Application
    Filed: September 27, 2004
    Publication date: May 17, 2007
    Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert Kwiatkowski, Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
  • Publication number: 20060252032
    Abstract: The present invention provides methods, compositions, and kits related to nucleic acid detection assays for detecting human herpes virus. For example, the present invention provides detection assays for detecting human herpes virus subtypes HHV1- through HHV-8.
    Type: Application
    Filed: January 30, 2006
    Publication date: November 9, 2006
    Applicant: Third Wave Technologies, Inc.
    Inventors: Azamat Aslanukov, Victor Lyamichev, Hatim Allawi, Vecheslav Elagin