Patents by Inventor Vecheslav Elagin
Vecheslav Elagin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9856514Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.Type: GrantFiled: November 18, 2014Date of Patent: January 2, 2018Assignee: Meridian Bioscience, Inc.Inventors: Vladimir I Slepnev, Ahmer Kodvawala, Brian Loeffler, Anand Hindupur, Reddy Ponaka, Vecheslav A. Elagin
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Publication number: 20170247680Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.Type: ApplicationFiled: April 4, 2017Publication date: August 31, 2017Inventors: Vladimir I. Slepnev, Ahmer Kodvawala, Brian Loeffler, Anand Hindupur, Reddy Ponaka, Vecheslav A. Elagin
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Patent number: 9249455Abstract: Disclosed are methods, kits, and components for detecting small RNA molecules, such as microRNA and siRNA, in a sample. The methods utilize primers and reporter molecules comprising non-natural bases. The disclosed kits may include one or more components for performing the disclosed methods.Type: GrantFiled: April 16, 2009Date of Patent: February 2, 2016Assignee: LUMINEX CORPORATIONInventors: Michael J. Moser, Vecheslav A. Elagin
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Publication number: 20150079601Abstract: The invention provides methods of detecting a nucleic acid present in a biological sample, comprising combining the biological sample with a lysis buffer to form a lysis mixture comprising nucleic acid released from cells in said biological sample; and subjecting a volume of the lysis mixture to size-exclusion chromatography in a column comprising a volume of size-exclusion medium. In certain embodiments, the lysis buffer separates double-stranded nucleic acid into single-stranded nucleic acid. In certain embodiments, the elution can have a flow rate of separation of less than 10 minutes to produce an eluted solution comprising isolated nucleic acid. The invention provides for a method of accurately and rapidly detecting products of nucleic acid amplification.Type: ApplicationFiled: November 18, 2014Publication date: March 19, 2015Inventors: Vladimir I. Slepnev, Ahmer Kodvawala, Brian Loeffler, Anand Hindupur, Reddy Ponaka, Vecheslav A. Elagin
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Patent number: 8765418Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: GrantFiled: February 15, 2013Date of Patent: July 1, 2014Assignee: Third Wave Technologies, Inc.Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Publication number: 20130149697Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: ApplicationFiled: February 15, 2013Publication date: June 13, 2013Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, JR., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Patent number: 8389245Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: GrantFiled: September 19, 2007Date of Patent: March 5, 2013Assignee: Third Wave Technologies, Inc.Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Jr., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Patent number: 8354232Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.Type: GrantFiled: July 20, 2010Date of Patent: January 15, 2013Assignee: Third Wave Technologies, Inc.Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor I. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
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Patent number: 8071750Abstract: The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.Type: GrantFiled: December 30, 2008Date of Patent: December 6, 2011Assignee: Third Wave Technologies, Inc.Inventors: Vecheslav A. Elagin, Scott M. Law, Bjork Hill
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Publication number: 20100285488Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.Type: ApplicationFiled: July 20, 2010Publication date: November 11, 2010Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor A. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
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Patent number: 7759062Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.Type: GrantFiled: June 11, 2007Date of Patent: July 20, 2010Assignee: Third Wave Technologies, Inc.Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor A. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
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Publication number: 20090275039Abstract: Disclosed are methods, kits, and components for detecting small RNA molecules, such as microRNA and siRNA, in a sample. The methods utilize primers and reporter molecules comprising non-natural bases. The disclosed kits may include one or more components for performing the disclosed methods.Type: ApplicationFiled: April 16, 2009Publication date: November 5, 2009Inventors: Michael J. Moser, Vecheslav A. Elagin
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Patent number: 7527948Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: GrantFiled: September 27, 2004Date of Patent: May 5, 2009Assignee: Third Wave Technologies, Inc.Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Jr., Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Publication number: 20090111092Abstract: The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.Type: ApplicationFiled: December 30, 2008Publication date: April 30, 2009Applicant: THIRD WAVE TECHNOLOGIES, INC.Inventors: Vecheslav A. Elagin, Scott M. Law, Bjork Hill
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Patent number: 7473773Abstract: The present invention provides compositions and methods for the detection and characterization of HCV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, to determine HCV genotype.Type: GrantFiled: January 7, 2005Date of Patent: January 6, 2009Assignee: Third Wave Technologies, Inc.Inventors: Vecheslav A. Elagin, Scott M. Law, Bjork Hill
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Publication number: 20080176215Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: ApplicationFiled: September 19, 2007Publication date: July 24, 2008Applicant: Third Wave Technologies, Inc.Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert W. Kwiatkowski, Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Publication number: 20080131870Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.Type: ApplicationFiled: June 11, 2007Publication date: June 5, 2008Applicant: Third Wave Technologies, Inc.Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor A. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
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Publication number: 20070207455Abstract: The present invention provides methods and compositions for detecting hepatitis C virus (HCV). In particular, the present invention provides nucleic acid detection assays configured to detect a novel sub-type of HCV-1.Type: ApplicationFiled: November 17, 2006Publication date: September 6, 2007Applicant: Third Wave Technologies, Inc.Inventors: Scott Law, Vecheslav Elagin
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Publication number: 20070111200Abstract: The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.Type: ApplicationFiled: September 27, 2004Publication date: May 17, 2007Inventors: Angela Hudson, Tamara Sander, Poonam Agarwal, Michelle Garsha, Hon Ip, Robert Kwiatkowski, Vecheslav Elagin, Marilyn Olson-Munoz, Michelle Curtis, Sarah Olson, Ilse Tyler
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Publication number: 20060252032Abstract: The present invention provides methods, compositions, and kits related to nucleic acid detection assays for detecting human herpes virus. For example, the present invention provides detection assays for detecting human herpes virus subtypes HHV1- through HHV-8.Type: ApplicationFiled: January 30, 2006Publication date: November 9, 2006Applicant: Third Wave Technologies, Inc.Inventors: Azamat Aslanukov, Victor Lyamichev, Hatim Allawi, Vecheslav Elagin