Patents by Inventor Veronika Aleksandrovna Kotliarova

Veronika Aleksandrovna Kotliarova has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11060121
    Abstract: The present invention provides a method for producing glycine or a salt thereof by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity. The bacterium can be, for example, a coryneform bacterium or a bacterium belonging to the family Enterobacteriaceae.
    Type: Grant
    Filed: June 23, 2020
    Date of Patent: July 13, 2021
    Assignee: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova
  • Publication number: 20200325507
    Abstract: The present invention provides a method for producing glycine or a salt thereof by fermentation of a bacterium which has been modified to overexpress a gene encoding a protein having L-threonine 3-dehydrogenase activity and a gene encoding a protein having 2-amino-3-oxobutanoate coenzyme A ligase activity. The bacterium can be, for example, a coryneform bacterium or a bacterium belonging to the family Enterobacteriaceae.
    Type: Application
    Filed: June 23, 2020
    Publication date: October 15, 2020
    Applicant: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova
  • Patent number: 10077447
    Abstract: The present invention provides a coryneform bacterium having an ability to produce a heterologous fusion protein by secretory production, which has been modified to express a genetic construct for secretory production of the heterologous fusion protein encoding at least a heterologous fusion protein comprising an extein and an intein having an activity of acyl rearrangement. The method for producing proteins modified at the C-terminus is also provided.
    Type: Grant
    Filed: October 27, 2015
    Date of Patent: September 18, 2018
    Assignee: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova, Hidemi Fujii, Masakazu Sugiyama
  • Publication number: 20160060640
    Abstract: The present invention provides a coryneform bacterium having an ability to produce a heterologous fusion protein by secretory production, which has been modified to express a genetic construct for secretory production of the heterologous fusion protein encoding at least a heterologous fusion protein comprising an extein and an intein having an activity of acyl rearrangement. The method for producing proteins modified at the C-terminus is also provided.
    Type: Application
    Filed: October 27, 2015
    Publication date: March 3, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova, Hidemi Fujii, Masakazu Sugiyama
  • Publication number: 20150203881
    Abstract: A method for producing an L-amino acid is described, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of a wild-type alcohol dehydrogenase encoded by the adhE gene or a mutant alcohol dehydrogenase which is resistant to aerobic inactivation.
    Type: Application
    Filed: March 25, 2015
    Publication date: July 23, 2015
    Applicant: AJINOMOTO CO., INC.
    Inventors: Leonid Romanovich Ptitsyn, Irina Borisovna Altman, Veronika Aleksandrovna Kotliarova, Olga Nikolaevna Mokhova, Tatyana Abramovna Yampolskaya, Yury Ivanovich Kozlov, Masaru Terashita, Yoshihiro Usuda, Kazuhiko Matsui
  • Patent number: 8524476
    Abstract: A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.
    Type: Grant
    Filed: June 21, 2010
    Date of Patent: September 3, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Olga Sergeevna Beznoschenko, Tatyana Aleksandrovna Bachina, Yuki Imabayashi, Masakazu Sugiyama, Shunichi Suzuki
  • Patent number: 8367381
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: February 5, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Sakayu Shimizu, Jun Ogawa, Makoto Hibi, Vitaly Grigorievich Paraskevov
  • Patent number: 8367382
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: February 5, 2013
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Sakayu Shimizu, Jun Ogawa, Makoto Hibi, Vitaly Grigorievich Paraskevov
  • Patent number: 8273562
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: January 5, 2012
    Date of Patent: September 25, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
  • Publication number: 20120115203
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Application
    Filed: January 5, 2012
    Publication date: May 10, 2012
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi, Vitaly Grigorievich Paraskevov
  • Publication number: 20120107883
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Application
    Filed: January 5, 2012
    Publication date: May 3, 2012
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
  • Publication number: 20120107884
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Application
    Filed: January 5, 2012
    Publication date: May 3, 2012
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
  • Patent number: 8114651
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Grant
    Filed: March 27, 2009
    Date of Patent: February 14, 2012
    Assignee: Ajinomoto Co., Inc.
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, legal representative, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
  • Publication number: 20100330622
    Abstract: A method for manufacturing a product of a reaction catalyzed by a protein having 2-oxoglutarate-dependent enzyme activity such as (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using a bacterium transformed with a DNA fragment containing a gene coding for a protein having 2-oxoglutarate-dependent enzyme activity such as L-isoleucine dioxygenase activity; and wherein said bacterium has the ability to produce a product such as (2S,3R,4S)-4-hydroxy-L-isoleucine.
    Type: Application
    Filed: June 21, 2010
    Publication date: December 30, 2010
    Inventors: Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Olga Sergeevna Beznoschenko, Tatyana Aleksandrovna Bachina, Yuki Imabayashi, Masakazu Sugiyama, Shunichi Suzuki
  • Publication number: 20100323409
    Abstract: A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof using an L-isoleucine-producing bacterium transformed with a DNA fragment containing a gene coding for a protein having L-isoleucine dioxygenase activity; and having the ability to produce (2S,3R,4S)-4-hydroxy-L-isoleucine.
    Type: Application
    Filed: June 21, 2010
    Publication date: December 23, 2010
    Inventors: Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Olga Sergeevna Beznoschenko
  • Publication number: 20090275092
    Abstract: A highly active L-isoleucine dioxygenase from Bacillus thuringiensis is provided. A method for manufacturing (2S,3R,4S)-4-hydroxy-L-isoleucine or a salt thereof by reacting L-isoleucine in an aqueous solvent in the presence of L-isoleucine dioxygenase and isolating (2S,3R,4S)-4-hydroxy-L-isoleucine is also provided.
    Type: Application
    Filed: March 27, 2009
    Publication date: November 5, 2009
    Inventors: Tomohiro Kodera, Sergey Vasilievich Smirnov, Natalia Nikolaevna Samsonova, Veronika Aleksandrovna Kotliarova, Natalia Yurievna Rushkevich, Yury Ivanovich Kozlov, Vitaly Grigorlevich Paraskevov, Sakayu Shimizu, Jun Ogawa, Makoto Hibi
  • Publication number: 20090203090
    Abstract: A method for producing an L-amino acid is described, for example L-threonine, L-lysine, L-histidine, L-phenylalanine, L-arginine, L-tryptophan, or L-glutamic acid, using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance an activity of a wild-type alcohol dehydrogenase encoded by the adhE gene or a mutant alcohol dehydrogenase which is resistant to aerobic inactivation.
    Type: Application
    Filed: January 15, 2009
    Publication date: August 13, 2009
    Inventors: LEONID ROMANOVICH PTITSYN, IRINA BORISOVNA ALTMAN, VERONIKA ALEKSANDROVNA KOTLIAROVA, OLGA NIKOLAEVNA MOKHOVA, TATYANA ABRAMOVNA YAMPOLSKAYA, Yury Ivanovich Kozlov, Vitaly Grigorievich Paraskevov, MASARU TERASHITA, YOSHIHIRO USUDA, KAZUHIKO MATSUI
  • Patent number: 7211419
    Abstract: L-arginine, citrulline and pyrimidine derivatives including orotic acid, uridine, uridine 5?-monophosphate (UMP), cytidine and cytidine 5?-monophosphate (CMP) are produced using a bacterium belonging to the genus Escherichia harboring a mutant carbamoylphosphate synthetase in which the amino acid sequence corresponding to positions from 947 to 951 in a wild type carbamoylphosphate synthetase is replaced with any one of amino acid sequences of SEQ ID NOS: 1 to 9, and feedback inhibition by uridine 5?-monophosphate in the bacterium is desensitized.
    Type: Grant
    Filed: October 20, 2005
    Date of Patent: May 1, 2007
    Assignee: Ajinomoto Co., Inc.
    Inventors: Leonid Romanovich Ptitsyn, Sergey Vasil'evich Smirnov, Irina Borisovna Altman, Anna Eugenievna Novikova, Veronika Aleksandrovna Kotliarova, Mikhail Markovich Gusyatiner, Yulia Georgievna Rostova, Tatyana Abramovna Yampolskaya
  • Patent number: 6991924
    Abstract: L-arginine, citrulline and pyrimidine derivatives including orotic acid, uridine, uridine 5?-monophosphate (UMP), cytidine and cytidine 5?-monophosphate (CMP) are produced using a bacterium belonging to the genus Escherichia harboring a mutant carbamoylphosphate synthetase in which the amino acid sequence corresponding to positions from 947 to 951 in a wild type carbamoylphosphate synthetase is replaced with any one of amino acid sequences of SEQ ID NOS: 1 to 9, and feedback inhibition by uridine 5?-monophosphate in the bacterium is desensitized.
    Type: Grant
    Filed: August 2, 2002
    Date of Patent: January 31, 2006
    Assignee: Ajinomoto Co., Inc.
    Inventors: Leonid Romanovich Ptitsyn, Sergey Vasil'evich Smirnov, Irina Borisovna Altman, Anna Eugenievna Novikova, Veronika Aleksandrovna Kotliarova, Mikhail Markovich Gusyatiner, Yulia Georgievna Rostova, Tatyana Abramovna Yampolskaya
  • Publication number: 20030129708
    Abstract: L-arginine, citrulline and pyrimidine derivatives including orotic acid, uridine, uridine 5′-monophosphate (UMP), cytidine and cytidine 5′-monophosphate (CMP) are produced using a bacterium belonging to the genus Escherichia harboring a mutant carbamoylphosphate synthetase in which the amino acid sequence corresponding to positions from 947 to 951 in a wild type carbamoylphosphate synthetase is replaced with any one of amino acid sequences of SEQ ID NOS: 1 to 9, and feedback inhibition by uridine 5′-monophosphate in the bacterium is desensitized.
    Type: Application
    Filed: November 12, 2002
    Publication date: July 10, 2003
    Applicant: AJINOMOTO CO., INC
    Inventors: Leonid Romanovich Ptitsyn, Sergey Vasil?apos;evich Smirnov, Irina Borisovna Altman, Anna Eugenievna Novikova, Veronika Aleksandrovna Kotliarova, Mikhail Markovich Gusyatiner, Yulia Georgievna Rostova, Tatyana Abramovna Yampolskaya