Patents by Inventor Victor I. Lyamichev

Victor I. Lyamichev has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9096893
    Abstract: The present invention provides systems, methods and kits for performing a detection assay (e.g., invasive cleavage assay) in combination with an amplification assay (e.g., PCR), where the detection assay employs enzyme footprint probes with relatively short (e.g., 6-12 bases) analyte-specific regions configured to provide a preferred footprint length of duplex for use with a particular nucleic acid modifying enzyme. In some embodiments, such assays are used for target quantification, and in other embodiments, such assays are used for genotyping. In certain embodiments, the use of such short probes allows for assays with increased dynamic range.
    Type: Grant
    Filed: July 12, 2013
    Date of Patent: August 4, 2015
    Assignee: HOLOGIC, INC.
    Inventors: Hatim T. Allawi, Victor I. Lyamichev
  • Publication number: 20140057259
    Abstract: The present invention provides systems, methods and kits for performing a detection assay (e.g., invasive cleavage assay) in combination with an amplification assay (e.g., PCR), where the detection assay employs enzyme footprint probes with relatively short (e.g., 6-12 bases) analyte-specific regions configured to provide a preferred footprint length of duplex for use with a particular nucleic acid modifying enzyme. In some embodiments, such assays are used for target quantification, and in other embodiments, such assays are used for genotyping. In certain embodiments, the use of such short probes allows for assays with increased dynamic range.
    Type: Application
    Filed: July 12, 2013
    Publication date: February 27, 2014
    Applicant: Hologic, Inc.
    Inventors: Hatim T. Allawi, Victor I. Lyamichev
  • Patent number: 8354232
    Abstract: The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: January 15, 2013
    Assignee: Third Wave Technologies, Inc.
    Inventors: Hatim Taysir Allawi, Vecheslav A. Elagin, Victor I. Lyamichev, Kwok Wu, Walter Iszczyszyn, Chris Fleming, LuAnne Chehak, Scott M. Law
  • Patent number: 8288093
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
    Type: Grant
    Filed: April 8, 2008
    Date of Patent: October 16, 2012
    Assignee: Third Wave Technologies, Inc.
    Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow
  • Patent number: 8182991
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to improved cleavage means for the detection and characterization of nucleic acid sequences. Structure-specific nucleases derived from a variety of thermostable organisms are provided. These structure-specific nucleases are used to cleave target-dependent cleavage structures, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: October 6, 2000
    Date of Patent: May 22, 2012
    Assignee: Third Wave Technologies, Inc.
    Inventors: Michael W. Kaiser, Victor I. Lyamichev, Natasha Lyamicheva
  • Patent number: 8026050
    Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5? nucleases and 3? exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
    Type: Grant
    Filed: September 13, 2000
    Date of Patent: September 27, 2011
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Mary Ann D. Brow, Victor I. Lyamichev
  • Patent number: 7829314
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: June 2, 2009
    Date of Patent: November 9, 2010
    Assignee: Third Wave Technologies, Inc.
    Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
  • Patent number: 7824862
    Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
    Type: Grant
    Filed: September 30, 2008
    Date of Patent: November 2, 2010
    Assignee: Third Wave Technologies, Inc.
    Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Todd A. Anderson, James E. Dahlberg
  • Patent number: 7790393
    Abstract: The present invention provides methods and routines for developing and optimizing nucleic acid detection assays for use in basic research, clinical research, and for the development of clinical detection assays. In particular, the present invention provides methods for designing oligonucleotide primers to be used in multiplex amplification reactions. The present invention also provides methods to optimize multiplex amplification reactions.
    Type: Grant
    Filed: July 16, 2008
    Date of Patent: September 7, 2010
    Assignee: Third Wave Technologies, Inc.
    Inventors: Victor I. Lyamichev, Andrew A. Lukowiak, Nancy Jarvis, David Kurensky
  • Patent number: 7785852
    Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5? nucleases and 3? exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
    Type: Grant
    Filed: August 5, 2005
    Date of Patent: August 31, 2010
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Mary Ann D. Brow, Victor I. Lyamichev
  • Patent number: 7691573
    Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5? nucleases and 3? exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
    Type: Grant
    Filed: August 5, 2005
    Date of Patent: April 6, 2010
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Mary Ann D. Brow, Victor I. Lyamichev
  • Publication number: 20090305283
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Application
    Filed: June 2, 2009
    Publication date: December 10, 2009
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
  • Patent number: 7601496
    Abstract: The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5? nucleases and 3? exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
    Type: Grant
    Filed: August 5, 2005
    Date of Patent: October 13, 2009
    Assignee: Third Wave Technologies, Inc.
    Inventors: James E. Dahlberg, Mary Ann D. Brow, Victor I. Lyamichev
  • Publication number: 20090253142
    Abstract: The present invention provides systems, methods and kits for performing a detection assay (e.g., invasive cleavage assay) in combination with an amplification assay (e.g., PCR), where the detection assay employs enzyme footprint probes with relatively short (e.g., 6-12 bases) analyte-specific regions configured to provide a preferred footprint length of duplex for use with a particular nucleic acid modifying enzyme. In some embodiments, such assays are used for target quantification, and in other embodiments, such assays are used for genotyping. In certain embodiments, the use of such short probes allows for assays with increased dynamic range.
    Type: Application
    Filed: March 13, 2009
    Publication date: October 8, 2009
    Applicant: HOLOGIC, INC.
    Inventors: Hatim T. Allawi, Victor I. Lyamichev
  • Patent number: 7588891
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: July 15, 2005
    Date of Patent: September 15, 2009
    Assignee: Third Wave Technologies, Inc.
    Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
  • Patent number: 7541145
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
    Type: Grant
    Filed: July 15, 2005
    Date of Patent: June 2, 2009
    Assignee: Third Wave Technologies, Inc.
    Inventors: James R. Prudent, Jeff G. Hall, Victor I. Lyamichev, Mary Ann D. Brow, James E. Dahlberg
  • Publication number: 20090117576
    Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
    Type: Application
    Filed: September 30, 2008
    Publication date: May 7, 2009
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Todd A. Anderson, James E. Dahlberg
  • Patent number: 7514220
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of nucleic acid from various viruses in a sample.
    Type: Grant
    Filed: October 28, 2007
    Date of Patent: April 7, 2009
    Assignee: Third Wave Technologies, Inc.
    Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow, Robert W. Kwiatkowski, Stephanie H. Vavra
  • Publication number: 20080268455
    Abstract: The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
    Type: Application
    Filed: April 8, 2008
    Publication date: October 30, 2008
    Applicant: THIRD WAVE TECHNOLOGIES, INC.
    Inventors: Jeff G. Hall, Victor I. Lyamichev, Andrea L. Mast, Mary Ann D. Brow
  • Patent number: 7429455
    Abstract: The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
    Type: Grant
    Filed: September 5, 2006
    Date of Patent: September 30, 2008
    Assignee: Third Wave Technologies, Inc.
    Inventors: Fang Dong, Victor I. Lyamichev, James R. Prudent, Lance Fors, Bruce P. Neri, Mary Ann D. Brow, Todd A. Anderson, James E. Dahlberg