Patents by Inventor Vladimir G. Budker

Vladimir G. Budker has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20040259828
    Abstract: Disclosed is a system for providing nucleic acid expression in a cell. A polynucleotide is inserted into a mammalian vasculature. The vessel permeability is increased and the polynucleotide is delivered to the cell where it enhances the endogenous properties of the cell.
    Type: Application
    Filed: May 4, 2004
    Publication date: December 23, 2004
    Inventors: Jon A. Wolff, Vladimir G. Budker, James E. Hagstrom, Sean D. Monahan, David B. Rozema, Paul M. Slattum
  • Devices and processes for distribution of genetic material to mammalian limb
    Publication number: 20040224879
    Abstract: A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from ischemia. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.
    Type: Application
    Filed: May 5, 2003
    Publication date: November 11, 2004
    Inventors: Jon A. Wolff, Vladimir G. Budker, James E. Hagstrom, Julia Hegge, Vladimir Subbotin
  • Method for single-step attachment of a label to target molecules
    Publication number: 20040210044
    Abstract: Compounds and methods are provided for a single-step covalent attachment of a label to a molecule comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Application
    Filed: April 15, 2003
    Publication date: October 21, 2004
    Inventors: Paul M. Slattum, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker
  • Process for generating multilayered particles
    Publication number: 20040203154
    Abstract: We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
    Type: Application
    Filed: March 8, 2004
    Publication date: October 14, 2004
    Inventors: Vladimir Trubetskoy, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, So Chun Wong, Jason Klein
  • Chelating systems for use in the delivery of compounds to cells
    Publication number: 20040197318
    Abstract: Chelator containing compounds are utilized in the delivery of molecules, polymers, nucleic acids and genes to animal cells. At least one chelator such as crown ether is attached to a polymer and then associated with another polymer such as DNA. An ion is then added to the mixture thereby forming condensed DNA. In condensed form and in complex with the chelator, DNA can be delivered to a cell.
    Type: Application
    Filed: April 22, 2004
    Publication date: October 7, 2004
    Inventors: Jon A. Wolff, Sean D. Monahan, James E. Hagstrom, Paul M. Slattum, Vladimir G. Budker, Vladimir Trubetskoy
  • Endosomolytic polymers
    Publication number: 20040198687
    Abstract: We describe pH-sensitive endosomolytic polymers, delivery particles containing pH-sensitive endosomolytic polymers. The described particles are capable of delivering polynucleotides to cells from the peripheral circulation with subsequent release from endosomes. The endosomolytic polymers are inactive outside the cell but disrupt membranes upon exposure to an acidified endosomal compartment.
    Type: Application
    Filed: April 1, 2004
    Publication date: October 7, 2004
    Inventors: David B. Rozema, Darren Wakefield, Jon A. Wolff, Vladimir Trubetskoy, Vladimir G. Budker, James E. Hagstrom, Aaron G. Loomis, Sean D. Monahan, Paul M. Slattum
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20040198678
    Abstract: Disclosed is a complex for providing nucleic acid expression in a cell. A polynucleotide and a polymer are mixed together to form the complex wherein the zeta potential of the complex is not positive. Then the complex is delivered to the cell wherein the nucleic acid is expressed.
    Type: Application
    Filed: June 7, 2001
    Publication date: October 7, 2004
    Inventors: Sean D. Monahan, Jon A. Wolff, Paul M. Slattum, James E. Hagstrom, Vladimir G. Budker, David B. Rozema
  • Polyampholytes for delivering polyions to a cell
    Patent number: 6794189
    Abstract: An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.
    Type: Grant
    Filed: May 10, 2002
    Date of Patent: September 21, 2004
    Assignee: Mirus Bio Corporation
    Inventors: Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, Vladimir S. Trubetskoy
  • Covalent modification of RNA for in vitro and in vivo delivery
    Publication number: 20040167090
    Abstract: The post-synthetic modification of RNA for the delivery of the RNA to a mammalian cell is described. The modifications enhance resistant of the RNA to nuclease digestion and delivery of the RNA to the cell whether the RNA is delivered alone or in combination with a transfection agent. Activity of the RNA in the cell is maintained.
    Type: Application
    Filed: February 19, 2004
    Publication date: August 26, 2004
    Inventors: Sean D. Monahan, Vladimir G. Budker, Lisa Nader, Vladimir Subbotin, Jon A. Wolff
  • Delivery of siRNA to cells using polyampholytes
    Publication number: 20040162235
    Abstract: A polyampholyte is utilized in a complex with siRNA for purposes of siRNA delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered a cell in vivo or in vitro.
    Type: Application
    Filed: February 18, 2003
    Publication date: August 19, 2004
    Inventors: Vladimir S. Trubetskoy, David B. Rozema, Sean D. Monahan, Vladimir G. Budker, James E. Hagstrom, Jon A. Wolff
  • Process of making a compound by forming a polymer from a template drug
    Publication number: 20040161463
    Abstract: A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.
    Type: Application
    Filed: January 12, 2004
    Publication date: August 19, 2004
    Inventors: Vladimir Trubetskoy, Jon A. Wolff, Paul M. Slattum, Lisa Hanson, Vladimir G. Budker, James E. Hagstrom
  • Protein and peptide delivery to mammalian cells in vitro
    Publication number: 20040151766
    Abstract: Compositions and methods for delivery of proteins and peptides to mammalian cells in vitro are described. Specifically, polypeptide-surfactant complexes formed from noncovalent hydrophobation of polypeptides and reversible hydrophobic modification of polypeptides are described. The compositions can be used to delivery positively charged, negatively charged and charge neutral polypeptides to cells.
    Type: Application
    Filed: January 29, 2004
    Publication date: August 5, 2004
    Inventors: Sean D. Monahan, Vladimir G. Budker, Kirk Ekena, Lisa Nader
  • Compounds and processes for single-pot attachment of a label to nucleic acid
    Publication number: 20040152084
    Abstract: Compounds and methods are provided for a single-pot covalent attachment of a label to nucleic acids comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Application
    Filed: January 31, 2003
    Publication date: August 5, 2004
    Inventors: Paul M. Slattum, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker
  • Compounds and processes for single-pot attachment of a label to siRNA
    Publication number: 20040146867
    Abstract: Compounds and methods are provided for a single-pot covalent attachment of a label to an siRNA comprising forming a covalently attachable labeling reagent for alkylating the molecule. Then, combining the covalently attachable labeling reagent with a mixture containing the molecule, under conditions wherein the labeling reagent has reactivity with the molecule thereby forming a covalent bond.
    Type: Application
    Filed: January 24, 2003
    Publication date: July 29, 2004
    Inventors: Paul M. Slattum, Vladimir G. Budker, James E. Hagstrom, Jon A. Wolff
  • Nucleic acid injected into hapatic vein lumen and delivered to primate liver
    Publication number: 20040110697
    Abstract: Processes are described for obtaining high levels of gene expression in primates after injection of nucleic acid to the liver via the lumen of the hepatic vein. The described process results in high level of gene expression with transient increases in liver enzymes.
    Type: Application
    Filed: December 5, 2002
    Publication date: June 10, 2004
    Inventors: Jon A. Wolff, Julia Hegge, James E. Hagstrom, Vladimir G. Budker
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20040106567
    Abstract: The process comprises designing a polynucleotide, such as an siRNA, for transfection. The polynucleotide is inserted into a mammalian vessel such as an artery. Prior to insertion, subsequent to insertion, or concurrent with insertion volume in the vessel is increased allowing the polynucleotide delivery to the parenchymal cell.
    Type: Application
    Filed: June 30, 2003
    Publication date: June 3, 2004
    Inventors: James E. Hagstrom, Jon A. Wolff, Sean D. Monahan, David B. Rozema, Vladimir G. Budker, Paul M. Slattum, David L. Lewis
  • Process for generating multilayered particles
    Patent number: 6740336
    Abstract: We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
    Type: Grant
    Filed: October 4, 2002
    Date of Patent: May 25, 2004
    Assignee: Mirus Corporation
    Inventors: Vladimir S. Trubetskoy, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, So Chun Wong, Jason Klein
  • Intravascular delivery of non-viral nucleic acid
    Publication number: 20040072785
    Abstract: A process is described for the delivery of a therapeutic polynucleotide to a tissue suffering from or potentially suffering from ischemia. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as an artery or a vein. Prior to insertion, subsequent to insertion, or concurrent with insertion the volume of the tissue is increased such that the genetic material is delivered to the parenchymal cell.
    Type: Application
    Filed: July 28, 2003
    Publication date: April 15, 2004
    Inventors: Jon A. Wolff, Sean D. Monahan, James E. Hagstrom, David B. Rozema, Vladimir G. Budker, Paul M. Slattum
  • Process for delivery of polynucleotides to the prostate
    Publication number: 20040067907
    Abstract: Disclosed is a system for providing in vivo delivery of polynucleotides to mammalian prostate cells using an intravascular administration route. The polynucleotides are inserted in an injection solution into a mammalian vasculature. Insertion of the injection solution at an appropriate rate increases the volume of extravascular fluid in the tissue thereby facilitating delivery of the polynucleotide to the cell.
    Type: Application
    Filed: October 6, 2003
    Publication date: April 8, 2004
    Inventors: James E. Hagstrom, Mark Noble, Julia Hegge, Vladimir G. Budker
  • Process for generating multilayered particles
    Publication number: 20040067587
    Abstract: We describe a process for generating multilayer particles comprising condensing a polymer with an oppositely charged polymer to form a particle and sequentially adding oppositely charged polymers to the particle forming at least three layers of polymers. The process is used to form a composition for delivering a biologically active compound to a cell.
    Type: Application
    Filed: October 4, 2002
    Publication date: April 8, 2004
    Inventors: Vladimir S. Trubetskoy, Jon A. Wolff, James E. Hagstrom, Vladimir G. Budker, So Chun Wong, Jason Klein