Abstract: The invention provides novel methods and compositions for introduction, transfer or delivery of one or more biomolecules into wounded recipient plant cell(s). Methods for production of a wounded recipient cell culture and the creation of one or more mutations, edits, transgenic insertions, or other genetic changes in the recipient cell(s) are also provided. Product cells produced by such methods, and resulting cells and regenerated plants, plant parts, and progeny plants are further provided. Molecular and genetic analyses, analysis of phenotypes and traits, and use of screenable and selection markers, are also provided to confirm transfer of the biomolecule in to the recipient cell(s) and generation of the mutation, edit, transgenic insertion, or other genetic change in the recipient cell(s), and/or progeny thereof, and in plants or plant parts developed or regenerated from the foregoing.

Abstract: The invention provides novel methods and compositions for transfer of nuclear and/or plastomic genomes, or portions thereof, or cytoplasmic component(s) and/or genetic material, between plant cells. Methods for production of a wounded mixed cell culture, or mixing two or more cell cultures after wounding, and transfer of genetic and/or cytoplasmic component(s), such as transfer of nuclear and/or plastid gene(s) or mutations, edits or alleles, between cells of the mixed culture, are also provided. Wounded mixed cell cultures produced by such methods, and resulting cells and regenerated plants, plant parts, and progeny plants are further provided. Molecular and genetic analyses, and screenable and selection markers, are also provided to confirm transfer and presence of cytoplasmic and/or nuclear component(s) and/or gene(s), mutation(s) or allele(s) in cells and plants produced by these methods.

Abstract: A method is provided for transforming monocotyledonous plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of monocot plant tissue with heterologous DNA constructs. The invention also provides for monocot cells in which the plastids contain heterologous DNA constructs.

Abstract: A method is provided for transforming monocotyledonous plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of monocot plant tissue with heterologous DNA constructs. The invention also provides for monocot cells in which the plastids contain heterologous DNA constructs.

Abstract: A method is provided for transforming monocotyledonous plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of monocot plant tissue with heterologous DNA constructs. The invention also provides for monocot cells in which the plastids contain heterologous DNA constructs.

Abstract: A method is provided for transforming monocotyledonous plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of monocot plant tissue with heterologous DNA constructs. The invention also provides for monocot cells in which the plastids contain heterologous DNA constructs.

Abstract: A method is provided for transforming maize plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of maize plant tissue with heterologous DNA constructs by microprojectile bombardment of green light grown cultures and dark grown embryogenic cultures. The invention also provides for maize cells in which the plastids contain heterologous DNA constructs.

Abstract: The present invention relates to a novel system for generating transformed dihaploid plants from haploid cells and tissues without the use of chromosome doubling agents.

Abstract: A method is provided for transforming maize plants to express DNA sequences of interest from plant cell plastids. The method allows the transformation of maize plant tissue with heterologous DNA constructs by microprojectile bombardment of green light grown cultures and dark grown embryogenic cultures. The invention also provides for maize cells in which the plastids contain heterologous DNA constructs.

Abstract: A compound containing at least two aromatic rings covalently bonded together, with each aromatic ring containing at least one oxyacetamide-based side chain, the compound being capable of forming a chloride ion channel across a lipid bilayer, and transporting chloride ion across the lipid bilayer.

Abstract: The present invention relates to a novel system for generating transformed dihaploid plants from haploid cells and tissues without the use of chromosome doubling agents.

Abstract: A compound containing at least two aromatic rings covalently bonded together, with each aromatic ring containing at least one oxyacetamide-based side chain, the compound being capable of forming a chloride ion channel across a lipid bilayer, and transporting chloride ion across the lipid bilayer.

Abstract: The present invention relates to a novel culture system for generating transformed corn plants from mature seeds. In particular, the invention relates to the use of plant hormones during germination to affect the culture response. Transgenic corn plants can then be easily produced.

Abstract: A method is provided for transforming solanaceous plants to express DNA sequences interest from the plant cell plastid. The improved method allows the transformation of solanaceous plant tissue which is not obtained from tobacco with DNA constructs. Such DNA constructs comprise, in the 5′ to 3′ direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The method can be utilized in the transformation of solanaceous plants, such as potato and petunia. The invention further provides constructs and methods for the expression of green fluorescent protein from the plant cell plastid.

Abstract: A method is provided for transforming solanaceous plants to express DNA sequences interest from the plant cell plastid. The improved method allows the transformation of solanaceous plant tissue which is not obtained from tobacco with DNA constructs. Such DNA constructs comprise, in the 5′ to 3′ direction of transcription, a promoter region functional in a plant plastid and a DNA sequence of interest. The method can be utilized in the transformation of solanaceous plants, such as potato and petunia. The invention further provides constructs and methods for the expression of green fluorescent protein from the plant cell plastid.