Patents by Inventor Walter Pfefferle

Walter Pfefferle has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7348037
    Abstract: The invention relates to sulfur-containing animal-feed additives produced from fermentation liquors, and to processes for their production.
    Type: Grant
    Filed: August 11, 2003
    Date of Patent: March 25, 2008
    Assignee: Evonik Degussa GmbH
    Inventors: Michael Buchholz, Michael Binder, Thomas Hermann, Walter Pfefferle, Georg Thierbach
  • Patent number: 7306939
    Abstract: The invention provides nucleotide sequences of the gpm gene which encode phosphoglycerate mutase, and fermentation processes for the preparation of amino acids, especially L-lysine, using corynebacteria wherein the gpm gene is amplified.
    Type: Grant
    Filed: November 29, 2000
    Date of Patent: December 11, 2007
    Assignee: Degussa GmbH
    Inventors: Bettina Möckel, Walter Pfefferle
  • Patent number: 7262036
    Abstract: Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
    Type: Grant
    Filed: May 22, 2006
    Date of Patent: August 28, 2007
    Assignee: Degussa AG
    Inventors: Bettina Hädrich, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
  • Patent number: 7252977
    Abstract: The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which encodes the sugar import protein K (msiK) gene, and a host-vector system having a coryneform host bacterium in which the msiK gene is present in enhanced form and a vector which carries at least the msiK gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: September 26, 2001
    Date of Patent: August 7, 2007
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Natalie Schischka, Mike Farwick, Walter Pfefferle
  • Publication number: 20070122832
    Abstract: The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Application
    Filed: December 22, 2006
    Publication date: May 31, 2007
    Inventors: Bettina Mockel, Mike Farwick, Thomas Hermann, Achim Marx, Walter Pfefferle
  • Patent number: 7205144
    Abstract: The invention related to an isolated nucleic acid consisting of a fragment of the polynucleotide of Corynebacterium glutamicum citA gene or a fragment of the full complement of the polynucleotide of the C. glutamicum citA gene wherein said fragment is at least 30 consecutive nucleotide in length, and the vector and host cell harboring these nucleic acids.
    Type: Grant
    Filed: November 1, 2004
    Date of Patent: April 17, 2007
    Assignee: Degussa AG
    Inventors: Bettina Mockel, Mike Farwick, Thomas Hermann, Achim Marx, Walter Pfefferle
  • Patent number: 7205131
    Abstract: The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no.2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the ptsH gene coding for component H of the phosphotransferase system, and the use of the above polynucleotides as primer or hybridisation probe.
    Type: Grant
    Filed: August 12, 2004
    Date of Patent: April 17, 2007
    Assignee: Degussa GmbH
    Inventors: Mike Farwick, Bettina Möckel, Walter Pfefferle
  • Patent number: 7202061
    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the mikE17 gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: August 24, 2004
    Date of Patent: April 10, 2007
    Assignee: Degussa GmbH
    Inventors: Mike Farwick, Klaus Hutchmacher, Walter Pfefferle
  • Publication number: 20070054380
    Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
    Type: Application
    Filed: June 19, 2006
    Publication date: March 8, 2007
    Inventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
  • Publication number: 20070054379
    Abstract: The present invention is directed to nucleotide sequences coding for phosphofructokinase which have been Corynebacterium glutamicum, and a process for the production of an L-amino acid comprising culturing a coryneform bacteria comprising an overexpressed pfk gene, wherein said pfk gene comprises a polynucleotide having a nucleotide sequence which is at least 90% identical to the nucleotide of SEQ ID NO: 1 encoding a polypeptide having the enzymatic activity of a phosphofructokinase, accumulating said L-amino acid in the medium or in the cells of said bacterium, and isolated said L-amino acid.
    Type: Application
    Filed: August 26, 2004
    Publication date: March 8, 2007
    Applicant: Degussa AG
    Inventors: Bettina Mockel, Walter Pfefferle
  • Patent number: 7160703
    Abstract: The present invention provides nucleotide sequences from Coryneform bacteria which code for the PtsI protein and a process for the fermentative preparation of amino acids using bacteria in which the ptsI gene is enhanced.
    Type: Grant
    Filed: June 13, 2003
    Date of Patent: January 9, 2007
    Assignee: Degussa AG
    Inventors: Bettina Moeckel, Stephan Hans, Natalie Schischka, Walter Pfefferle
  • Patent number: 7144724
    Abstract: A process for the production of an L-amino acid wherein coryneform bacteria (e.g. Coryneform glutamicum) in which expression of the mqo gene coding for malate quinone oxidoreductase is attenuated are fermented to produce a desired amino acid, and the amino acid is concentrated in the medium or cells and isolated. Optionally, further genes in the biosynthetic pathway of the desired amino acid are enhanced, and/or metabolic pathways that reduce formation of the amino acid are suppressed.
    Type: Grant
    Filed: November 2, 2005
    Date of Patent: December 5, 2006
    Assignee: Degussa AG
    Inventors: Mike Farwick, Brigitte Bathe, Thomas Hermann, Achim Marx, Walter Pfefferle
  • Patent number: 7132272
    Abstract: The present invention relates to an isolated polynucleotide coding for the lrp gene and to a process for the fermentative production of L-amino acids by altering the expression of this gene.
    Type: Grant
    Filed: April 30, 2002
    Date of Patent: November 7, 2006
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Jörn Kalinowski, Alfred Pühler, Bettina Möckel, Walter Pfefferle
  • Patent number: 7129066
    Abstract: The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide that is at least 70% identical with a polynucleotide that codes for a polypeptide containing the amino acid sequence of SEQ ID No. 2, b) polynucleotide that codes for a polypeptide containing an amino acid sequence that is at least 70% identical with the amino acid sequence of SEQ ID No. 2, c) polynucleotide that is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of a), b) or c), and to a process for the production of L-amino acids by fermentation using coryneform bacteria in which at least the citE gene is present in attenuated form, and to the use of polynucleotides containing the sequences of the invention as hybridization probes.
    Type: Grant
    Filed: February 28, 2003
    Date of Patent: October 31, 2006
    Assignee: Degussa AG
    Inventors: Mike Farwick, Klaus Huthmacher, Achim Marx, Brigitte Bathe, Walter Pfefferle
  • Patent number: 7118904
    Abstract: The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.
    Type: Grant
    Filed: December 8, 2003
    Date of Patent: October 10, 2006
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Thomas Hermann, Alfred Pühler, Jörn Kalinowski, Brigitte Bathe
  • Publication number: 20060205046
    Abstract: Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
    Type: Application
    Filed: May 22, 2006
    Publication date: September 14, 2006
    Inventors: Bettina Mockel, Walter Pfefferle, Achim Marx, Jorn Kalinowski, Brigitte Bathe, Alfred Puhler
  • Patent number: 7105321
    Abstract: The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.
    Type: Grant
    Filed: December 2, 2003
    Date of Patent: September 12, 2006
    Assignee: Degussa AG
    Inventors: Bettina Moeckel, Caroline Kreutzer, Thomas Hermann, Mike Farwick, Achim Marx, Walter Pfefferle
  • Patent number: 7094584
    Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
    Type: Grant
    Filed: March 19, 2004
    Date of Patent: August 22, 2006
    Assignee: Degussa AG
    Inventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Möckel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
  • Publication number: 20060177912
    Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the dep34 gene, and a host-vector system having a coryneform host bacterium in which the dep34 gene is present in attenuated form and a vector which carries at least the dep34 gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Application
    Filed: November 16, 2005
    Publication date: August 10, 2006
    Inventors: Mike Farwick, Klaus Huthmacher, Walter Pfefferle, Thomas Hermann, Brigitte Bathe
  • Patent number: 7078502
    Abstract: Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.
    Type: Grant
    Filed: April 6, 2001
    Date of Patent: July 18, 2006
    Assignee: Degussa AG
    Inventors: Bettina Moeckel, Mike Farwick, Thomas Hermann, Caroline Kreutzer, Walter Pfefferle