Patents by Inventor Walter Pfefferle

Walter Pfefferle has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7074607
    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.
    Type: Grant
    Filed: August 14, 2003
    Date of Patent: July 11, 2006
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
  • Patent number: 7049106
    Abstract: A process for the production of an L-amino acid wherein coryneform bacteria (e.g. Coryneform glutamicum) in which expression of the mqo gene coding for malate quinone oxidoreductase is attenuated are fermented to produce a desired amino acid, and the amino acid is concentrated in the medium or cells and isolated. Optionally, further genes in the biosynthetic pathway of the desired amino acid are enhanced, and/or metabolic pathways that reduce formation of the amino acid are suppressed.
    Type: Grant
    Filed: April 9, 2002
    Date of Patent: May 23, 2006
    Assignee: Degussa AG
    Inventors: Mike Farwick, Brigitte Bathe, Thomas Hermann, Achim Marx, Walter Pfefferle
  • Patent number: 7038034
    Abstract: The invention provides nucleotide sequences from Coryneform bacteria coding for the dep33 efflux protein and a process for the fermentative preparation of amino acids using bacteria in which the dep33 efflux protein is attenuated.
    Type: Grant
    Filed: September 10, 2001
    Date of Patent: May 2, 2006
    Assignee: Degussa AG
    Inventors: Mike Farwick, Klaus Huthmacher, Walter Pfefferle, Thomas Hermann, Brigitte Bathe
  • Patent number: 7037689
    Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the sigC gene from Corynebacterium glutamicum, and a host-vector system having a coryneform host bacterium, such as Corynebacterium glutamicum, and a vector which carries at least the sigC gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: August 30, 2001
    Date of Patent: May 2, 2006
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Stephan Hans, Mike Farwick, Thomas Hermann, Walter Pfefferle
  • Patent number: 7029904
    Abstract: The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which codes for the dep34 efflux protein, and a host-vector system having a coryneform host bacterium in which the dep34 gene is present in attenuated form and a vector which carries at least the dep34 gene according to SEQ ID NO: 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: September 6, 2001
    Date of Patent: April 18, 2006
    Assignee: Degussa AG
    Inventors: Mike Farwick, Klaus Huthmacher, Walter Pfefferle, Thomas Hermann, Brigitte Bathe
  • Patent number: 7026158
    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the mikE17 gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: April 4, 2001
    Date of Patent: April 11, 2006
    Assignee: Degussa AG
    Inventors: Mike Farwick, Klaus Huthmacher, Walter Pfefferle
  • Publication number: 20060040317
    Abstract: The invention relates to a process for the production of L-amino acids, in which the following steps are carried out: a) fermentation of the coryneform bacteria producing the desired L-amino acid, in which at least the mqo gene is attenuated, b) concentration of the desired L-amino acid in the medium or in the cells of the bacteria, and c) isolation of the L-amino acid, and, optionally, bacteria are used in which further genes of the biosynthesis pathway of the desired L-amino acid are additionally enhanced, or bacteria are used in which at least some of the metabolic pathways reducing formation of the desired L-amino acid are excluded.
    Type: Application
    Filed: November 2, 2005
    Publication date: February 23, 2006
    Applicant: DEGUSSA AG
    Inventors: Mike Farwick, Brigitte Bathe, Thomas Hermann, Achim Marx, Walter Pfefferle
  • Patent number: 6995000
    Abstract: The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which encodes the sigma factor M (sigM) gene, and a host-vector system having a coryneform host bacterium in which the sigM gene is present in enhanced form and a vector which carries at least the sigM gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: August 31, 2001
    Date of Patent: February 7, 2006
    Assignee: Degussa
    Inventors: Brigitte Bathe, Christine Bastuck, Mike Farwick, Thomas Hermann, Walter Pfefferle
  • Publication number: 20060019357
    Abstract: An isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the rpsL gene is present in enhanced form, as well as the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Application
    Filed: August 5, 2005
    Publication date: January 26, 2006
    Applicant: DEGUSSA AG
    Inventors: Bettina Moeckel, Brigitte Bathe, Stefan Hans, Caroline Kreutzer, Thomas Hermann, Walter Pfefferle, Michael Binder
  • Patent number: 6987015
    Abstract: An isolated polynucleotide containing a polynucleotide sequence selected from the following group: a) a polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID NO:2, b) a polynucleotide which encodes a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID NO:2, c) a polynucleotide which is complementary to the polynucleotides of a) or b), and d) a polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), a process for the fermentative production of L-amino acids with amplification of the pfkA gene and use as primer or hybridisation probe.
    Type: Grant
    Filed: November 20, 2000
    Date of Patent: January 17, 2006
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Walter Pfefferle
  • Publication number: 20050282259
    Abstract: Isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the lysR2 gene is present in attenuated form, and the use of the polynucleotide sequences as hybridization probes.
    Type: Application
    Filed: July 12, 2005
    Publication date: December 22, 2005
    Applicant: Degussa AG
    Inventors: Bettina Moeckel, Mike Farwick, Thomas Hermann, Caroline Kreutzer, Walter Pfefferle
  • Publication number: 20050271768
    Abstract: The invention relates to sulfur-containing animal-feed additives produced from fermentation liquors, and to processes for their production.
    Type: Application
    Filed: August 11, 2003
    Publication date: December 8, 2005
    Applicant: DEGUSSA AG
    Inventors: Michael Buchholz, Michael Binder, Thomas Hermann, Walter Pfefferle, Georg Thierbach
  • Patent number: 6972190
    Abstract: The invention relates to an isolated polynucleotide from Corynebacterium glutamicum having a polynucleotide sequence which codes for the carbon starvation protein A (cstA) gene, and a host-vector system having a coryneform host bacterium in which the cstA gene is present in enhanced form and a vector which carries at least the cstA gene according to SEQ ID NO: 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: August 24, 2001
    Date of Patent: December 6, 2005
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Achim Marx, Walter Pfefferle, Mike Farwick, Thomas Hermann
  • Publication number: 20050266534
    Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the cstA gene, and a host-vector system having a coryneform host bacterium in which the cstA gene is present in attenuated form and a vector which carries at least the cstA gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.
    Type: Application
    Filed: June 8, 2005
    Publication date: December 1, 2005
    Inventors: Bettina Mockel, Achim Marx, Walter Pfefferle, Mike Farwick, Thomas Hermann
  • Publication number: 20050266535
    Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.
    Type: Application
    Filed: June 29, 2005
    Publication date: December 1, 2005
    Applicant: DEGUSSA AG
    Inventors: Brigitte Bathe, Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Michael Binder, Dieter Greissinger, Georg Thierbach, Alfred Puehler
  • Patent number: 6958228
    Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: October 25, 2005
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Publication number: 20050233373
    Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.
    Type: Application
    Filed: June 20, 2005
    Publication date: October 20, 2005
    Applicant: DEGUSSA AG
    Inventors: Brigitte Bathe, Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Publication number: 20050233424
    Abstract: Nucleotide sequences from coryneform bacteria which code for the cysD, cysN, cysK, cysE and cysH genes and a process for the fermentative preparation of amino acids using bacteria in which the genes mentioned are enhanced, a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the cysD gene, the cysN gene, the cysK gene, the cysE gene and/or the cysH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes and a process for the preparation of an L-methionine-containing animal feedstuffs additive from fermentation broths.
    Type: Application
    Filed: July 26, 2004
    Publication date: October 20, 2005
    Applicant: DEGUSSA AG
    Inventors: Mike Farwick, Klaus Huthmacher, Natalie Schischka, Brigitte Bathe, Walter Pfefferle, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Publication number: 20050221450
    Abstract: The present invention relates to an isolated polynucleotide from Corynebacterium glutamicum comprising a polynucleotide sequence chosen from the group consisting of (a) a polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO: 2; (b) a polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO: 2; (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b); and (d) a polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of (a), (b), or (c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the sigE gene is present in enhanced form, and the use of polynucleotides which comprise the sequence according to the invention as hybridization probes.
    Type: Application
    Filed: May 26, 2005
    Publication date: October 6, 2005
    Inventors: Bettina Mockel, Thoams Hermann, Mike Farwick, Michael Binder, Walter Pfefferle
  • Patent number: 6949374
    Abstract: The invention relates to a genetically modified coryneform bacterium, the fadD15 gene of which is amplified, and an isolated polynucleotide which codes for acyl-CoA synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the fadD15 gene in the bacteria and the use of the polynucleotide as a primer or hybridization probe.
    Type: Grant
    Filed: May 16, 2001
    Date of Patent: September 27, 2005
    Assignee: Degussa AG
    Inventors: Madhavan Nampoothiri K., Bettina Möckel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm