Patents by Inventor Walter Pfefferle

Walter Pfefferle has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nucleotide sequences which code for the ACP gene
    Patent number: 6830921
    Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), a process for the fermentative preparation of L-amino acids with enhancement of the acp gene and the use of the polynucleotide as a primer or hybridization probe.
    Type: Grant
    Filed: May 10, 2001
    Date of Patent: December 14, 2004
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Anke Weissenborn, Walter Pfefferle, Hermann Sahm, Lothar Eggeling, Madhavan Nampoothiri
  • Nucleotide sequences encoding a sensor kinase, citA, from corynebacterium glutamicum
    Patent number: 6825030
    Abstract: The present invention is related to nucleotide sequences encoding a sensor kinse, citA, from Corynebacterium glutamicum.
    Type: Grant
    Filed: March 13, 2001
    Date of Patent: November 30, 2004
    Assignee: Degussa AG
    Inventors: Bettina Möckel, Mike Farwick, Thomas Hermann, Achim Marx, Walter Pfefferle
  • Nucleotide sequences which code for the cysD, cysN, cysK, cysE and cysH genes
    Patent number: 6822085
    Abstract: Nucleotide sequences from coryneform bacteria which code for the cysD, cysN, cysK, cysE and cysH genes and a process for the fermentative preparation of amino acids using bacteria in which the genes mentioned are enhanced, a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the cysD gene, the cysN gene, the cysK gene, the cysE gene and/or the cysH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes and a process for the preparation of an L-methionine-containing animal feedstuffs additive from fermentation broths.
    Type: Grant
    Filed: September 26, 2001
    Date of Patent: November 23, 2004
    Assignee: Degussa AG
    Inventors: Mike Farwick, Klaus Huthmacher, Natalie Schischka, Brigitte Bathe, Walter Pfefferle, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Nucleotide sequences encoding the ptsH gene
    Patent number: 6818432
    Abstract: The invention relates to an isolated polynucleotide containing a polynucleotide sequence selected from the group comprising a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no.2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the ptsH gene coding for component H of the phosphotransferase system, and the use of the above polynucleotides as primer or hybridisation probe.
    Type: Grant
    Filed: March 29, 2001
    Date of Patent: November 16, 2004
    Assignee: Degussa AG
    Inventors: Mike Farwick, Bettina Mockel, Walter Pfefferle
  • Nucleotide sequences encoding o-succinylhomoserine sulfhydrylase
    Patent number: 6815196
    Abstract: The present invention relates to isolated polynucleotides from Corynebacterium glutamicum encoding proteins of the transcription activator (MetR) and O-succinylhomoserine sulfhydrylase (MetZ). The invention also relates to producing L-amino acids, particularly methionine, in coryneform cells having attenuated metR and metZ genes.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: November 9, 2004
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Nucleotide sequences which code for the eno gene
    Publication number: 20040220394
    Abstract: The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.
    Type: Application
    Filed: December 8, 2003
    Publication date: November 4, 2004
    Applicant: Degussa AG
    Inventors: Bettina Mockel, Walter Pfefferle, Thomas Hermann, Alfred Puhler, Jorn Kalinowski, Brigitte Bathe
  • Nucleotide sequences which code for the metY gene
    Patent number: 6812016
    Abstract: The present invention relates to isolated polynucleotides which encode proteins with O-acetylhomoserine sulfhydrylase, vectors and cells containing the polynucleotides as well as methods of preparing L-amino acids.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: November 2, 2004
    Assignee: Degussa AG
    Inventors: Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Nucleotide sequences which code for the ccpa2 gene
    Publication number: 20040209285
    Abstract: The invention relates to polynucleotides corresponding to the ccpA2 gene and which encode a CcpA2 catabolite control protein, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having CcpA2 catabolite control activity.
    Type: Application
    Filed: December 2, 2003
    Publication date: October 21, 2004
    Applicant: DEGUSSA AG
    Inventors: Bettina Moeckel, Caroline Kreutzer, Thomas Hermann, Mike Farwick, Achim Marx, Walter Pfefferle
  • Nucleotide sequences which encode the pfk gene
    Patent number: 6806068
    Abstract: The present invention is directed to nucleotide sequences coding for phosphofructokinase which have been derived from Corynebacterium glutamicum.
    Type: Grant
    Filed: November 20, 2000
    Date of Patent: October 19, 2004
    Assignee: Degussa AG Dusseldorf
    Inventors: Bettina Möckel, Walter Pfefferle
  • Nucleotide sequences which code for the rpoB gene
    Publication number: 20040180359
    Abstract: The present invention relates to polynucleotides corresponding to the rpoB gene and which encode the &bgr;-subunit of RNA polymerase B, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having activity of the &bgr;-subunit of RNA polymerase B.
    Type: Application
    Filed: November 13, 2003
    Publication date: September 16, 2004
    Applicant: DEGUSSA AG
    Inventors: Bettina Moeckel, Brigitte Bathe, Thomas Hermann, Walter Pfefferle, Michael Binder
  • Nucleotide sequences which code for the rpoB gene
    Patent number: 6783967
    Abstract: The present invention relates to polynucleotides corresponding to the rpoB gene and which encode the &bgr;-subunit of RNA polymerase B, methods of producing L-amino acids, and methods of screening for polynucleotides which encode proteins having activity of the &bgr;-subunit of RNA polymerase B.
    Type: Grant
    Filed: June 25, 2001
    Date of Patent: August 31, 2004
    Assignee: Degussa AG
    Inventors: Bettina Moeckel, Brigitte Bathe, Thomas Hermann, Walter Pfefferle, Michael Binder
  • Nucleotide sequences which code for the RodA protein
    Patent number: 6777206
    Abstract: The invention provides nucleotide sequences from Coryneform bacteria which code for the RodA protein and a process for the fermentative preparation of amino acids using bacteria in which the rodA gene is enhanced.
    Type: Grant
    Filed: September 12, 2001
    Date of Patent: August 17, 2004
    Assignee: Degussa AG
    Inventors: Mike Farwick, Klaus Huthmacher, Walter Pfefferle, Brigitte Bathe
  • Process for the preparation of L-amino acids by attenuating the sucC and sucD genes
    Publication number: 20040152166
    Abstract: The invention relates to polynucleotides that contain polynucleotide sequences coding for the genes sucC and sucD, selected from the group
    Type: Application
    Filed: March 17, 2004
    Publication date: August 5, 2004
    Applicant: Degussa AG
    Inventors: Bettina Mockel, Walter Pfefferle, Achim Marx
  • Nucleotide sequences which code for the sahH gene
    Patent number: 6759224
    Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which at least the sahH gene is present in enhanced form, and the use of polynucleotides which contain the sequences according to the invention as hybridization probes.
    Type: Grant
    Filed: August 2, 2001
    Date of Patent: July 6, 2004
    Assignee: Degussa AG
    Inventors: Mike Farwick, Klaus Huthmacher, Jennifer Brehme, Walter Pfefferle, Michael Binder, Dieter Greissinger, Georg Thierbach
  • Nucleotide sequences coding for the glbO gene
    Patent number: 6759218
    Abstract: The present invention provides an isolated polynucleotide containing a polynucleotide sequence selected from the group a) polynucleotide which is at least 70% identical to a polynucleotide which codes for a polypeptide containing the amino acid sequence of SEQ ID no. 2, b) polynucleotide which codes for a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative production of L-amino acids with enhancement of the glbO gene which codes for the haemoglobin-like protein and the use of the above polynucleotides as a primer or hybridization probe.
    Type: Grant
    Filed: May 7, 2002
    Date of Patent: July 6, 2004
    Assignee: Degussa-AG
    Inventors: Bettina Mockel, Achim Marx, Walter Pfefferle
  • Nucleotide sequences encoding histidine kinase from corynebacterium glutamicum
    Patent number: 6746854
    Abstract: This invention relates to novel polynucleotide sequences encoding the histidine kinase luminescence expression sensor (luxS) gene from Corynebaclerium glutamicum, probes to the novel polynucleotide sequences encoding the luxS gene, vector and host cells containing the novel luxS polynucleotide sequences, the encoded Lux S polypeptide, and a process for the fermentative preparation of amino acids using bacteria which the luxS gene is attenuated.
    Type: Grant
    Filed: August 1, 2001
    Date of Patent: June 8, 2004
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Caroline Kreutzer, Achim Marx, Walter Pfefferle
  • L-lysine-producing corynebacteria and process for the preparation of L-lysine
    Patent number: 6746855
    Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
    Type: Grant
    Filed: January 8, 2003
    Date of Patent: June 8, 2004
    Assignees: Dégussa-Hüls Aktiengesellschaft, Forschungszentrum Jülich GmbH
    Inventors: Caroline Kreutzer, Stephan Hans, Mechthild Rieping, Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm, Miroslav Patek
  • Nucleotide sequences encoding the dapC gene and process for the production of L-lysine
    Patent number: 6740742
    Abstract: The invention relates to an isolated polynucleotide from coryneform bacteria wherein the polynucleotide encodes a protein having n-succinylaminokeptopimelate transaminase activity.
    Type: Grant
    Filed: March 22, 2001
    Date of Patent: May 25, 2004
    Assignee: Degussa AG
    Inventors: Bettina Mockel, Anke Weissenborn, Walter Pfefferle, Michael Hartmann, Jörn Kalinowski, Alfred Pühler
  • Nucleotide sequences coding for the cdsA gene
    Publication number: 20040092710
    Abstract: This invention relates to a genetically modified coryneform bacterium, the cdsA gene of which is amplified, and to an isolated polynucleotide, which codes for phosphatidate cytidylyl transferase from coryneform bacteria and to a method for the fermentative production of L-amino acids with amplification of the cdsA gene in the bacteria and to the use of the polynucleotide as a primer or hybridization probe.
    Type: Application
    Filed: May 14, 2001
    Publication date: May 13, 2004
    Inventors: Madhavan Nampoothiri K., Bettina Mockel, Walter Pfefferle, Lothar Eggeling, Hermann Sahm
  • Nucleotide sequences coding for the chrS protein
    Patent number: 6734002
    Abstract: The invention provides nucleotide sequences from Coryneform bacteria coding for the ChrS protein and a process for the fermentative preparation of amino acids using bacteria in which the ChrS protein is attenuated.
    Type: Grant
    Filed: September 10, 2001
    Date of Patent: May 11, 2004
    Assignee: Degussa AG
    Inventors: Brigitte Bathe, Natalie Schischka, Achim Marx, Walter Pfefferle