Patents by Inventor Werner Hoelke
Werner Hoelke has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20230052545Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: ApplicationFiled: August 26, 2022Publication date: February 16, 2023Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Patent number: 11459556Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: GrantFiled: May 4, 2020Date of Patent: October 4, 2022Assignee: Roche Diagnostics Operations, Inc.Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Publication number: 20200263160Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: ApplicationFiled: May 4, 2020Publication date: August 20, 2020Applicant: Roche Diagnostics Operations, Inc.Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Publication number: 20170247679Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: ApplicationFiled: May 15, 2017Publication date: August 31, 2017Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Patent number: 9574186Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: GrantFiled: April 28, 2015Date of Patent: February 21, 2017Assignee: Roche Diagnostics Operations, Inc.Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber
-
Publication number: 20160319268Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: ApplicationFiled: April 29, 2015Publication date: November 3, 2016Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Publication number: 20150337282Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: ApplicationFiled: April 28, 2015Publication date: November 26, 2015Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber
-
Patent number: 9045743Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilizate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: GrantFiled: June 5, 2012Date of Patent: June 2, 2015Assignee: Roche Diagnostic Operations, Inc.Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber
-
Patent number: 8476053Abstract: The present invention provides an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase. The present invention further provides a method of stabilizing an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase, and a method of preparing a purified aqueous composition comprising the protein with enzymatic activity of alpha-galactosidase.Type: GrantFiled: April 19, 2010Date of Patent: July 2, 2013Assignee: Roche Diagnostics Operations, Inc.Inventors: Harald Sobek, Manfred Schmidt, Rainer Mueller, Werner Hoelke, Johann-Peter Thalhofer
-
Publication number: 20120244598Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: ApplicationFiled: June 5, 2012Publication date: September 27, 2012Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber
-
Patent number: 8236356Abstract: The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferably plant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.Type: GrantFiled: June 4, 2009Date of Patent: August 7, 2012Assignee: Roche Diagnostics Operations, Inc.Inventors: Bernhard Suppmann, Werner Hoelke, Artur Hoffmann, Thomas Marx, Kirsten Sonn, Johann-Peter Thalhofer
-
Patent number: 8211684Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: GrantFiled: August 17, 2009Date of Patent: July 3, 2012Assignee: Roche Diagnostics Operations, Inc.Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber
-
Publication number: 20120003714Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysis. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: ApplicationFiled: September 13, 2011Publication date: January 5, 2012Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Patent number: 7956167Abstract: The present invention provides a method for purifying Clostridium histolyticum collagenase type I and type II proteins from a complex mixture by subsequently performing a precipitation with ammonium sulfate, hydrophobic interaction chromatography, cation exchange chromatography, and anion chromatography. Conditions are provided which lead to a stabilized, partially purified preparation even after the precipitation step. The method of the invention leads to a quick and efficient removal of other proteolytic activities. The preparations according to the invention provide exceptionally pure and intact collagenase type I and type II proteins which are enzymatically active. The invention also provides blends of the two isolated proteins. The invention further provides the use of the purified collagenase proteins or blends thereof for treating a tissue sample in vitro.Type: GrantFiled: May 29, 2009Date of Patent: June 7, 2011Assignee: Roche Diagnostics Operations, Inc.Inventors: Werner Hoelke, Hellmut Eckstein, Michaela Fischer, Antje Liehre, Bernhard Suppmann, Johann-Peter Thalhofer
-
Publication number: 20110070622Abstract: The present invention provides a method for purifying Clostridium histolyticum collagenase type I and type II proteins from a complex mixture by subsequently performing a precipitation with ammonium sulfate, hydrophobic interaction chromatography, cation exchange chromatography, and anion chromatography. Conditions are provided which lead to a stabilized, partially purified preparation even after the precipitation step. The method of the invention leads to a quick and efficient removal of other proteolytic activities. The preparations according to the invention provide exceptionally pure and intact collagenase type I and type II proteins which are enzymatically active. The invention also provides blends of the two isolated proteins. The invention further provides the use of the purified collagenase proteins or blends thereof for treating a tissue sample in vitro.Type: ApplicationFiled: May 29, 2009Publication date: March 24, 2011Inventors: Werner Hoelke, Hellmut Eckstein, Michaela Fischer, Antje Liehre, Bernhard Suppmann, Johann-Peter Thalhofer
-
Publication number: 20100273235Abstract: The present invention provides an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase. The present invention further provides a method of stabilizing an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase, and a method of preparing a purified aqueous composition comprising the protein with enzymatic activity of alpha-galactosidase.Type: ApplicationFiled: April 19, 2010Publication date: October 28, 2010Inventors: Harald Sobek, Manfred Schmidt, Rainer Mueller, Werner Hoelke, Johann-Peter Thalhofer
-
Publication number: 20100248326Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: ApplicationFiled: August 17, 2009Publication date: September 30, 2010Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber
-
Publication number: 20100086971Abstract: The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferably plant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.Type: ApplicationFiled: June 4, 2009Publication date: April 8, 2010Inventors: Bernhard Suppmann, Werner Hoelke, Artur Hoffmann, Thomas Marx, Kirsten Sonn, Johann-Peter Thalhofer
-
Patent number: 7482145Abstract: By deglycosylating an enzyme derived from a eukaryotic organism prior to forming a conjugate, i.e. prior to attaching the enzyme to a molecule capable of binding to a target molecule, the sensitivity of an assay for detecting the presence or determining the quantity of a target molecule, in which the conjugate is used as a labelled component, can be increased. The present invention provides conjugates, methods for providing the same, use of such conjugates as well as kits containing the conjugates.Type: GrantFiled: September 15, 2005Date of Patent: January 27, 2009Assignee: Roche Diagnostics Operations, Inc.Inventors: Wolfgang Hoesel, Werner Hoelke, Beata Kanne, Helmut Lenz, Rainer Mueller
-
Patent number: 7368274Abstract: The invention concerns a method for the expression of a gene coding for a soluble proteinase K in yeast e.g. in Pichia pastoris with subsequent secretion into the culture medium. In addition a method for purifying the heterologously expressed and secreted proteinase K is described.Type: GrantFiled: February 5, 2002Date of Patent: May 6, 2008Assignee: Roche Diagnostics Operations, Inc.Inventors: Rainer Mueller, Johann-Peter Thalhofer, Frank Geipel, Stephan Glaser, Werner Hoelke, Helmut Schoen, Thomas Kirschbaum