Patents by Inventor William V. Burnett

William V. Burnett has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20120015438
    Abstract: The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise at least two temperature shifts performed during the culturing period, in which the temperature is lower at the end of the culturing period than at the time of initial cell culture. Throughout their duration, the culturing processes of the invention involving two or more downward shifts in temperature sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein. In another aspect, the methods comprise the delayed addition of polyanionic compound during the culturing period.
    Type: Application
    Filed: March 18, 2009
    Publication date: January 19, 2012
    Inventors: Bernhard M. Schilling, Linda Matlock, Stephen G. Zegarelli, William V. Burnett, Christoph E. Joosten, Jonathan D. Basch, Sivakesava Sakhamuri, Steven S. Lee
  • Patent number: 7541164
    Abstract: The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise at least two temperature shifts performed during the culturing period, in which the temperature is lower at the end of the culturing period than at the time of initial cell culture. Throughout their duration, the culturing processes of the invention involving two or more downward shifts in temperature sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein. In another aspect, the methods comprise the delayed addition of polyanionic compound during the culturing period.
    Type: Grant
    Filed: December 18, 2003
    Date of Patent: June 2, 2009
    Assignee: Bristol-Myers Squibb Company
    Inventors: Bernhard M. Schilling, Linda Matlock, Stephen G. Zegarelli, William V. Burnett, Christoph E. Joosten, Jonathan D. Basch, Sivakesava Sakhamuri, Steven S. Lee
  • Patent number: 6942990
    Abstract: The invention provides a glutaryl 7-ACA amidase from Pseudomonas diminuta strain BS-203, which catalyzes the hydrolysis of 7-?-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.
    Type: Grant
    Filed: January 17, 2003
    Date of Patent: September 13, 2005
    Assignee: Bristol Myers Squibb Company
    Inventors: Ross Binder, Joanne L. Brown, Thomas J. Franceschini, William V. Burnett, Michael Politino, Suo Win Liu, Sean M. Tonzi
  • Publication number: 20030143715
    Abstract: The invention provides a glutaryl 7-ACA amidase from Pseudomonas diminuta strain BS-203, which catalyzes the hydrolysis of 7-&bgr;-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.
    Type: Application
    Filed: January 17, 2003
    Publication date: July 31, 2003
    Inventors: Ross Binder, Joanne L. Brown, Thomas J. Franceschini, William V. Burnett, Michael Politino, Suo Win Liu, Sean M. Tonzi
  • Patent number: 6528300
    Abstract: The invention provides a glutaryl 7-ACA amidase from Pseudomonas diminuta strain BS-203, which catalyzes the hydrolysis of 7-&bgr;-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.
    Type: Grant
    Filed: September 21, 2001
    Date of Patent: March 4, 2003
    Assignee: Bristol-Myers Squibb Co.
    Inventors: Ross Binder, Joanne L. Brown, Thomas J. Franceschini, William V. Burnett, Michael Politino, Suo Win Liu, Sean M. Tonzi
  • Publication number: 20020160500
    Abstract: The invention provides a glutaryl 7-ACA amidase from Pseudomonas diminuta strain BS-203, which catalyzes the hydrolysis of 7-&bgr;-(4-carboxybutanamido)-cephalosporanic acid to 7-aminocephalosporanic acid and glutaric acid. The invention also provides nucleic acid sequences, vectors, and host cells useful in the production of this amidase. The glutaryl 7-ACA amidase can be used for the preparation of 7-aminocephalosporanic acid.
    Type: Application
    Filed: September 21, 2001
    Publication date: October 31, 2002
    Inventors: Ross Binder, Joanne L. Brown, Thomas J. Franceschini, William V. Burnett, Michael Politino, Suo Win Liu, Sean M. Tonzi
  • Patent number: 5869309
    Abstract: The instant invention provides nucleic acid molecules encoding cephalosporin esterase from Rhodosporidium toruloides. In addition, the invention provides isolated cephalosporin esterase, expression vectors, host cells, and methods of producing cephalosporin esterase.
    Type: Grant
    Filed: September 17, 1997
    Date of Patent: February 9, 1999
    Assignee: Bristol-Myers Squibb Co.
    Inventors: Michael Politino, Sean M. Tonzi, John J. Usher, William V. Burnett, Guna Romancik
  • Patent number: 5516679
    Abstract: Nucleic acids coding for penicillin V amidohydrolase (PVA) from Fusarium oxysporum. Also, expression vectors, host cells and a method for production of PVA.
    Type: Grant
    Filed: December 23, 1994
    Date of Patent: May 14, 1996
    Assignee: Bristol-Myers Squibb Company
    Inventors: Shu-Jen Chiang, William V. Burnett, Jr., Sean M. Tonzi
  • Patent number: 4865982
    Abstract: A DNA fragment from Streptomyces sp. which contains the XP55 gene expression unit.
    Type: Grant
    Filed: May 29, 1985
    Date of Patent: September 12, 1989
    Assignee: SmithKline Beckman Corporation
    Inventors: William V. Burnett, Jr., Thomas G. Eckhardt, Louis R. Fare
  • Patent number: 4859601
    Abstract: A DNA fragment from Streptomyces sp. which contains the Bgl protein (P49) promoter, the P1 promoter, for expressing heterologous genes.
    Type: Grant
    Filed: January 21, 1986
    Date of Patent: August 22, 1989
    Assignee: SmithKline Beckman Corporation
    Inventors: William V. Burnett, Jr., Thomas G. Eckhardt, Louis R. Fare