Patents by Inventor Wolfgang Aehle
Wolfgang Aehle has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11130946Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: January 14, 2020Date of Patent: September 28, 2021Assignee: Danisco US Inc.Inventors: Pieter Van Solingen, Richard R. Bott, Wolfgang Aehle, Martijn Scheffers
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Publication number: 20200270595Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: January 14, 2020Publication date: August 27, 2020Inventors: PIETER VAN SOLINGEN, RICHARD R. BOTT, WOLFGANG AEHLE, MARTIJN SCHEFFERS
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Patent number: 10563187Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: March 28, 2018Date of Patent: February 18, 2020Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
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Publication number: 20190194577Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.Type: ApplicationFiled: September 7, 2018Publication date: June 27, 2019Inventors: LUIS G. CASCAO-PEREIRA, DAVID A. ESTELL, FRITS GOEDEGEBUUR, JAMES T. KELLIS, JR., AYROOKARAN J. POULOSE, BRIAN F. SCHMIDT, WOLFGANG AEHLE
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Publication number: 20190002853Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: March 28, 2018Publication date: January 3, 2019Applicant: Danisco US Inc.Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
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Patent number: 10093887Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.Type: GrantFiled: July 21, 2014Date of Patent: October 9, 2018Assignee: DANISCO US INC.Inventors: Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, Jr., Ayrookaran J. Poulose, Brian F. Schmidt
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Patent number: 9944913Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: August 5, 2016Date of Patent: April 17, 2018Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
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Publication number: 20170114336Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: ApplicationFiled: August 5, 2016Publication date: April 27, 2017Applicant: Danisco US Inc.Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
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Patent number: 9523087Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.Type: GrantFiled: February 25, 2014Date of Patent: December 20, 2016Assignee: DANISCO US INCInventors: Wolfgang Aehle, Richard R. Bott, Benjamin S. Bower, Jonathan Caspi, David A. Estell, Frits Goedegebuur, Ronaldus Wilhelmus Joannes Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Johannes Franciscus Thomas Van Lieshout, Igor Nikolaev, Sander Van Stigt Thans, Louise Wallace, Gudrun Vogtentanz, Mats Sandgren
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Patent number: 9469848Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly, the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: June 30, 2011Date of Patent: October 18, 2016Assignee: DUPONT NUTRITION BIOSCIENCES APSInventors: Peter Edvard Degn, Richard R. Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle, Elin Petersen
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Patent number: 9447397Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: October 9, 2007Date of Patent: September 20, 2016Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn S. Scheffers, Richard R. Bott
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Patent number: 9334466Abstract: Variants of Bacillus sp. no. 707 alpha-amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.Type: GrantFiled: August 23, 2013Date of Patent: May 10, 2016Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Neelam S. Amin
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Patent number: 9290753Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: February 4, 2014Date of Patent: March 22, 2016Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Martijn Scheffers, Pieter van Solingen, Casper Vroemen
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Publication number: 20160010072Abstract: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.Type: ApplicationFiled: June 29, 2015Publication date: January 14, 2016Inventors: Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, Ayrookaran J. Poulose, Brian F. Schmidt
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Publication number: 20150132825Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.Type: ApplicationFiled: November 10, 2014Publication date: May 14, 2015Applicant: DANISCO US INC.Inventors: Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, JR., Ayrookaran J. Poulose, Brian F. Schmidt
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Patent number: 9029116Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.Type: GrantFiled: August 30, 2013Date of Patent: May 12, 2015Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikoaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
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Publication number: 20150072913Abstract: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.Type: ApplicationFiled: September 11, 2014Publication date: March 12, 2015Applicant: DANISCO US INC.Inventors: Wolfgang Aehle, David A. Estell, Ronaldus W.J. Hommes, Brian E. Jones, Marc Kolkman, Chris Leeflang, Hiroshi Oh, Ayrookaran J. Poulose, Andrew Shaw, Wilhelmus A.H. Van der Kley, Leonardus P.M. Van Marrewijk
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Publication number: 20150031589Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.Type: ApplicationFiled: July 21, 2014Publication date: January 29, 2015Applicant: DANISCO US INC.Inventors: Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, JR., Ayrookaran J. Poulose, Brian F. Schmidt
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Patent number: 8865449Abstract: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.Type: GrantFiled: May 6, 2013Date of Patent: October 21, 2014Assignee: Danisco US Inc.Inventors: Wolfgang Aehle, David A. Estell, Ronaldus W. J. Hommes, Brian E. Jones, Marc Kolkman, Chris Leeflang, Hiroshi Oh, Ayrookaran J. Poulose, Andrew Shaw, Wilhelmus A. H. Van der Kley, Leonardus P. M. Van Marrewijk
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Publication number: 20140302585Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.Type: ApplicationFiled: February 25, 2014Publication date: October 9, 2014Applicant: DANISCO US INC.Inventors: Wolfgang AEHLE, Richard R. BOTT, Benjamin S. BOWER, Jonathan CASPI, David A. ESTELL, Frits GOEDEGEBUUR, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, Johannes Franciscus Thomas VAN LIESHOUT, Igor NIKOLAEV, Sander VAN STIGT THANS, Louise WALLACE, Gudrun VOGTENTANZ, Mats SANDGREN