Patents by Inventor Wolfgang Aehle

Wolfgang Aehle has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11130946
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: January 14, 2020
    Date of Patent: September 28, 2021
    Assignee: Danisco US Inc.
    Inventors: Pieter Van Solingen, Richard R. Bott, Wolfgang Aehle, Martijn Scheffers
  • Publication number: 20200270595
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Application
    Filed: January 14, 2020
    Publication date: August 27, 2020
    Inventors: PIETER VAN SOLINGEN, RICHARD R. BOTT, WOLFGANG AEHLE, MARTIJN SCHEFFERS
  • Patent number: 10563187
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: March 28, 2018
    Date of Patent: February 18, 2020
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
  • Publication number: 20190194577
    Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
    Type: Application
    Filed: September 7, 2018
    Publication date: June 27, 2019
    Inventors: LUIS G. CASCAO-PEREIRA, DAVID A. ESTELL, FRITS GOEDEGEBUUR, JAMES T. KELLIS, JR., AYROOKARAN J. POULOSE, BRIAN F. SCHMIDT, WOLFGANG AEHLE
  • Publication number: 20190002853
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Application
    Filed: March 28, 2018
    Publication date: January 3, 2019
    Applicant: Danisco US Inc.
    Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
  • Patent number: 10093887
    Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
    Type: Grant
    Filed: July 21, 2014
    Date of Patent: October 9, 2018
    Assignee: DANISCO US INC.
    Inventors: Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, Jr., Ayrookaran J. Poulose, Brian F. Schmidt
  • Patent number: 9944913
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: August 5, 2016
    Date of Patent: April 17, 2018
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
  • Publication number: 20170114336
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Application
    Filed: August 5, 2016
    Publication date: April 27, 2017
    Applicant: Danisco US Inc.
    Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn Scheffers, Richard Bott
  • Patent number: 9523087
    Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
    Type: Grant
    Filed: February 25, 2014
    Date of Patent: December 20, 2016
    Assignee: DANISCO US INC
    Inventors: Wolfgang Aehle, Richard R. Bott, Benjamin S. Bower, Jonathan Caspi, David A. Estell, Frits Goedegebuur, Ronaldus Wilhelmus Joannes Hommes, Thijs Kaper, Bradley R. Kelemen, Slavko Kralj, Johannes Franciscus Thomas Van Lieshout, Igor Nikolaev, Sander Van Stigt Thans, Louise Wallace, Gudrun Vogtentanz, Mats Sandgren
  • Patent number: 9469848
    Abstract: The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly, the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: June 30, 2011
    Date of Patent: October 18, 2016
    Assignee: DUPONT NUTRITION BIOSCIENCES APS
    Inventors: Peter Edvard Degn, Richard R. Bott, Casper Willem Vroemen, Martijn Silvan Scheffers, Wolfgang Aehle, Elin Petersen
  • Patent number: 9447397
    Abstract: The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: October 9, 2007
    Date of Patent: September 20, 2016
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Piet Van Solingen, Martijn S. Scheffers, Richard R. Bott
  • Patent number: 9334466
    Abstract: Variants of Bacillus sp. no. 707 alpha-amylase are provided that are produced more efficiently and thus more economically. Higher fermentation yields are achieved through introducing amino acid variations that promote solubility of the variant in a fermentation broth. Increased solubility allows more enzyme to remain in solution after expression in a host cell. This in turn increases the efficiency with which the expressed variant enzyme can be recovered from the fermentation broth.
    Type: Grant
    Filed: August 23, 2013
    Date of Patent: May 10, 2016
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Neelam S. Amin
  • Patent number: 9290753
    Abstract: The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: February 4, 2014
    Date of Patent: March 22, 2016
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Richard R. Bott, Martijn Scheffers, Pieter van Solingen, Casper Vroemen
  • Publication number: 20160010072
    Abstract: The present invention provides engineered protease variants. In particular, the protease variants comprise combinable mutations at selected surface positions that affect the charge and/or hydrophobicity of the enzyme to enhance at least one desired property of the resulting variant enzyme in a chosen application. Compositions comprising the protease variants, and methods for using the same are also provided.
    Type: Application
    Filed: June 29, 2015
    Publication date: January 14, 2016
    Inventors: Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, Ayrookaran J. Poulose, Brian F. Schmidt
  • Publication number: 20150132825
    Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
    Type: Application
    Filed: November 10, 2014
    Publication date: May 14, 2015
    Applicant: DANISCO US INC.
    Inventors: Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, JR., Ayrookaran J. Poulose, Brian F. Schmidt
  • Patent number: 9029116
    Abstract: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.
    Type: Grant
    Filed: August 30, 2013
    Date of Patent: May 12, 2015
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, Richard R. Bott, Igor Nikoaev, Martijn Scheffers, Piet Van Solingen, Casper Vroemen
  • Publication number: 20150072913
    Abstract: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.
    Type: Application
    Filed: September 11, 2014
    Publication date: March 12, 2015
    Applicant: DANISCO US INC.
    Inventors: Wolfgang Aehle, David A. Estell, Ronaldus W.J. Hommes, Brian E. Jones, Marc Kolkman, Chris Leeflang, Hiroshi Oh, Ayrookaran J. Poulose, Andrew Shaw, Wilhelmus A.H. Van der Kley, Leonardus P.M. Van Marrewijk
  • Publication number: 20150031589
    Abstract: The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.
    Type: Application
    Filed: July 21, 2014
    Publication date: January 29, 2015
    Applicant: DANISCO US INC.
    Inventors: Wolfgang Aehle, Luis G. Cascao-Pereira, David A. Estell, Frits Goedegebuur, James T. Kellis, JR., Ayrookaran J. Poulose, Brian F. Schmidt
  • Patent number: 8865449
    Abstract: The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.
    Type: Grant
    Filed: May 6, 2013
    Date of Patent: October 21, 2014
    Assignee: Danisco US Inc.
    Inventors: Wolfgang Aehle, David A. Estell, Ronaldus W. J. Hommes, Brian E. Jones, Marc Kolkman, Chris Leeflang, Hiroshi Oh, Ayrookaran J. Poulose, Andrew Shaw, Wilhelmus A. H. Van der Kley, Leonardus P. M. Van Marrewijk
  • Publication number: 20140302585
    Abstract: The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
    Type: Application
    Filed: February 25, 2014
    Publication date: October 9, 2014
    Applicant: DANISCO US INC.
    Inventors: Wolfgang AEHLE, Richard R. BOTT, Benjamin S. BOWER, Jonathan CASPI, David A. ESTELL, Frits GOEDEGEBUUR, Ronaldus Wilhelmus HOMMES, Thijs KAPER, Bradley R. KELEMEN, Slavko KRALJ, Johannes Franciscus Thomas VAN LIESHOUT, Igor NIKOLAEV, Sander VAN STIGT THANS, Louise WALLACE, Gudrun VOGTENTANZ, Mats SANDGREN