Patents by Inventor Wolfgang Wohlleben

Wolfgang Wohlleben has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5637489
    Abstract: Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: June 10, 1997
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Eckhard Strauch, Wolfgang Wohlleben, Walter Arnold, Renate Alijah, Alfred Puhler, Gerhard Wohner, Rudiger Marquardt, Susanne Grabley, Dieter Brauer, Klaus Bartsch
  • Patent number: 5633154
    Abstract: A method of locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves:(1) The construction of a non-self-transferrable vector mobilizable from an E. coli mobilizer strain which vector is composed of(a) A DNA segment containing a replicon functional in E. coli,(b) A second DNA segment containing the DNA fragment coding for the mobilization function (Mob site containing the oriT),(c) A third DNA segment which recombines homologously in Gram-positive bacteria and/or contains a replicon functional in coryneform bacteria,(d) A DNA segment from Bacillus subtilis containing the sacB gene,(2) Transfer of this vector by means of conjugative transfer into the coryneform recipient strains,(3) Cultivation of the transconjugants containing the vector in an .about.
    Type: Grant
    Filed: November 4, 1994
    Date of Patent: May 27, 1997
    Assignee: Degussa Aktiengesellschaft
    Inventors: Andreas Schaefer, Anna-Hildegard Seep-Feldhaus, Wolfgang Jaeger, Joern Kalinowski, Wolfgang Wohlleben, Alfred Puehler
  • Patent number: 5380657
    Abstract: A method locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves:(1) The construction of a non-self-transferrable vector mobilizable from an E. coli mobilizer strain which vector is composed of(a) A DNA segment containing a replicon functional in E. coli,(b) A second DNA segment containing the DNA fragment coding for the mobilization function (Mob site containing the oriT),(c) A third DNA segment which recombines homologously in Gram-positive bacteria and/or contains a replicon functional in coryneform bacteria,(d) A DNA segment from Bacillus subtilis containing the sacB gens,(2) Transfer of this vector by means of conjugative transfer into the coryneform recipient strains,(3) Cultivation of the transconjugants containing the vector in an .about.
    Type: Grant
    Filed: March 18, 1993
    Date of Patent: January 10, 1995
    Assignee: Degussa Aktiengesellschaft
    Inventors: Andreas Schaefer, Anna-Hildegard Seep-Feldhaus, Wolfgang Jaeger, Joern Kalinowski, Wolfgang Wohlleben, Alfred Puehler
  • Patent number: 5336607
    Abstract: The kil-kor system from the plasmid pIJ101 can be utilized for regulated gene expression in Streptomycetes. For this, the KorA protein is either inactivated or eliminated to "switch on" a particular gene.
    Type: Grant
    Filed: July 23, 1993
    Date of Patent: August 9, 1994
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Wolfgang Wohlleben, Gunter Muth, Alfred Puhler, Gunther J. Rieb
  • Patent number: 5328998
    Abstract: The invention relates to a promoter screening vector, to methods for the identification and isolation of Streptomyces promoters using the screening vector, and to the isolated promoters themselves, preferably the pS1 and P14 (SEQ2ID NO: 1) promoters of the S. ghanaensis phase I19.
    Type: Grant
    Filed: August 7, 1992
    Date of Patent: July 12, 1994
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Gabriele Labes, Wolfgang Wohlleben
  • Patent number: 5276268
    Abstract: The phosphinothricin (PTC)-resistance gene isolated from the genome of Streptomyces viridochromogenes DSM 40736 is, after adaptation to the codon usage in plants, synthesized and incorporated into gene structures which make plants resistant to PTC after expression therein.
    Type: Grant
    Filed: July 29, 1991
    Date of Patent: January 4, 1994
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Eckhard Strauch, Walter Arnold, Renate Alijah, Wolfgang Wohlleben, Alfred Puhler, Peter Eckes, Gunter Donn, Eugen Uhlmann, Friedrich Hein, Friedrich Wengenmayer
  • Patent number: 5273894
    Abstract: Selection of Streptomyces viridochromogenes DSM 4112 for resistance to phosphinothricyl-alanyl-alanine (PTT) results in PTT-resistance selectants. The DNA fragment which carries the phosphinothricin(PTC)-resistance gene is obtained from the total DNA of these selectants by cutting with BamHI, cloning of a fragment 4.0 kb in size, and selection for PTT resistance. This gene is suitable for the production of PTC-resistant plants, and as a resistance marker and for the selective N-acetylation of the L-form of racemic PTC.
    Type: Grant
    Filed: November 20, 1991
    Date of Patent: December 28, 1993
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Eckhard Strauch, Wolfgang Wohlleben, Walter Arnold, Renate Alijah, Alfred Puhler, Gerhard Wohner, Rudiger Marquardt, Susanne Grabley, Dieter Brauer, Klaus Bartsch
  • Patent number: 4992371
    Abstract: Starting from the Streptomycetes plasmids pSG2, pSG5 and pSVH1, "shuttle vectors" which, in additon to a E. coli replicon, also contain suitable selection markers for E. coli and Streptomycetes are constructed. These shuttle vectors replicate both in E. coli and in Streptomycetes, and they can be used for cloning and expression of foreign DNA.
    Type: Grant
    Filed: June 26, 1989
    Date of Patent: February 12, 1991
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Wolfgang Wohlleben, Gunter Muth, Alfred Puhler
  • Patent number: 4965207
    Abstract: Intensely crimson colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of Streptomyces lividans. Re-isolation of the plasmid DNA and cutting with BamHI results in fragments about 3.4 to 9 kb in length, on which is located the gene which determines production of the coloring agent. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.
    Type: Grant
    Filed: August 11, 1987
    Date of Patent: October 23, 1990
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Gunter Muth, Wolfgang Wohlleben, Alfred Puhler, Gerhard Wohner, Rudiger Marquardt
  • Patent number: 4963496
    Abstract: Intensely blue-black colonies are obtained from Streptomyces coelicolor DSM 3030 by total digestion of the total DNA, cloning into a suitable vector, and transformation of a streptomycetes recipient strain. Re-isolation of the plasmid DNA and cutting with BamHI results in a 5.5 kb fragment on which the gene for the coloring agent is located. This gene is suitable as marker, in particular as inactivation marker, in Streptomycetes.
    Type: Grant
    Filed: August 11, 1987
    Date of Patent: October 16, 1990
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Gunter Muth, Wolfgang Wohlleben, Alfred Puhler, Gerhard Wohner, Rudiger Marquardt
  • Patent number: 4918015
    Abstract: S. ghanaensis DSM 2932 is resistant to gentamicin at up to 20 .mu.g/ml. Total digestion of the genomic DNA with BglII, incorporation of the restriction fragments into a suitable plasmid, and selection using gentamicin results in gentamicin-resistant clones which contain a 7 kb fragment with the gentamicin-resistance gene. The plasmid pPH1JI likewise contains a gentamicin-resistance gene located on a 2.3 kb HindIII-BamHI fragment. These genes are suitable as markers, in particular for Streptomycetes vectors.
    Type: Grant
    Filed: April 30, 1987
    Date of Patent: April 17, 1990
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Wolfgang Wohlleben, Gunter Muth, Alfred Puhler
  • Patent number: 4880746
    Abstract: The plasmid pSG5 which, because of its molecular length of about 12.7 kb and its four unique restriction sites, can be used for the construction of plasmid vectors for Streptomycetes, for example of shuttle vectors between Streptomycetes and other microorganisms, can be isolated from a culture of Streptomyces ghanaensis DSM 2932.
    Type: Grant
    Filed: October 14, 1987
    Date of Patent: November 14, 1989
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Wolfgang Wohlleben, Agnes Schulte, Alfred Puhler
  • Patent number: 4621061
    Abstract: The new streptomyces plasmid p SG 2, having a molecular weight of 9.2 megadaltons, a contour length of 4.58 .mu.m and a molecular length of about 13.8 kilobases, is described, together with its preparation from a culture of "Streptomyces ghanaensis" ATCC 14 672.
    Type: Grant
    Filed: December 13, 1983
    Date of Patent: November 4, 1986
    Assignee: Hoechst Aktiengesellschaft
    Inventors: Alfred Puhler, Wolfgang Wohlleben, Michael Leineweber