Patents by Inventor Xing-Fang Li

Xing-Fang Li has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 12104150
    Abstract: The present disclosure relates generally to a near-infrared photothermally activated CRISPR/CAS9 genome editing machine.
    Type: Grant
    Filed: September 14, 2020
    Date of Patent: October 1, 2024
    Assignee: The Governors of the University of Alberta
    Inventors: Hanyong Peng, Hongquan Zhang, Xing-Fang Li, Xiaochun Le
  • Patent number: 11819822
    Abstract: A silica monolith nested in a polymer sponge may be formed by applying a hydrolyzed mixture of siloxanes to a melamine-formaldehyde sponge, and may be used in methods of solid phase extraction.
    Type: Grant
    Filed: January 31, 2020
    Date of Patent: November 21, 2023
    Assignee: The Governors of the University of Alberta
    Inventors: Xing-Fang Li, Zhongshan Liu
  • Publication number: 20230313322
    Abstract: On-site viral inactivation and RNA preservation of gargle and saliva samples combined with direct analysis of SARS-COV2 RNA on magnetic beads.
    Type: Application
    Filed: February 3, 2023
    Publication date: October 5, 2023
    Inventors: Xiaochun Chris LE, Xing-Fang Li, D. Lorne Tyrrell, Yanming Liu, Teresa Kumblathan
  • Publication number: 20210079373
    Abstract: The present disclosure relates generally to a near-infrared photothermally activated CRISPR/CAS9 genome editing machine.
    Type: Application
    Filed: September 14, 2020
    Publication date: March 18, 2021
    Inventors: Hanyong PENG, Hongquan ZHANG, Xing-Fang LI, Xiaochun LE
  • Patent number: 10934162
    Abstract: There is described herein a nanomotor system and methods of use.
    Type: Grant
    Filed: November 15, 2017
    Date of Patent: March 2, 2021
    Assignee: THE GOVERNORS OF THE UNIVERSITY OF ALBERTA
    Inventors: X. Chris Le, Hongquan Zhang, Hanyong Peng, Xing-Fang Li
  • Publication number: 20200246778
    Abstract: A silica monolith nested in a polymer sponge may be formed by applying a hydrolyzed mixture of siloxanes to a melamine-formaldehyde sponge, and may be used in methods of solid phase extraction.
    Type: Application
    Filed: January 31, 2020
    Publication date: August 6, 2020
    Inventors: Zhongshan Liu, Xing-Fang Li
  • Patent number: 10526608
    Abstract: The present disclosure relates generally to method of preparing aptamers, aptamers, and uses thereof.
    Type: Grant
    Filed: October 30, 2017
    Date of Patent: January 7, 2020
    Assignee: The Governors of the University of Alberta
    Inventors: Zhixin Wang, Hongquan Zhang, Xing-Fang Li, Xiaochun Le
  • Patent number: 10155797
    Abstract: The invention provides a binding-induced DNA nanomachine that can be activated by proteins and nucleic acids. This new type of nanomachine hamesses specific target binding to trigger assembly of separate DNA components that are otherwise unable to spontaneously assemble. Three-dimensional DNA tracks of high density are constructed on gold nanoparticles functionalized with hundreds of single-stranded oligonucleotides and tens of an affinity ligand. A DNA swing arm, free in solution, can be linked to a second affinity ligand. Binding of a target molecule to the two ligands brings the swing arm to AuNP and initiates autonomous, stepwise movement of the swing arm around the AuNP surface. The movement of the swing arm generates hundreds of oligonucleotides in response to a single binding event. The new nanomachines have several unique and advantageous features over DNA nanomachines that rely on DNA self-assembly.
    Type: Grant
    Filed: September 12, 2016
    Date of Patent: December 18, 2018
    Assignee: The Governors of the University of Alberta
    Inventors: Hongquan Zhang, Xiaochun Le, Xing-Fang Li
  • Publication number: 20180134549
    Abstract: There is described herein a nanomotor system and methods of use.
    Type: Application
    Filed: November 15, 2017
    Publication date: May 17, 2018
    Inventors: X. Chris LE, Hongquan ZHANG, Hanyong PENG, Xing-Fang LI
  • Publication number: 20180119149
    Abstract: The present disclosure relates generally to method of preparing aptamers, aptamers, and uses thereof.
    Type: Application
    Filed: October 30, 2017
    Publication date: May 3, 2018
    Inventors: Zhixin WANG, Hongquan ZHANG, Xing-Fang LI, Xiaochun LE
  • Publication number: 20170073682
    Abstract: The invention provides a binding-induced DNA nanomachine that can be activated by proteins and nucleic acids. This new type of nanomachine hamesses specific target binding to trigger assembly of separate DNA components that are otherwise unable to spontaneously assemble. Three-dimensional DNA tracks of high density are constructed on gold nanoparticles functionalized with hundreds of single-stranded oligonucleotides and tens of an affinity ligand. A DNA swing arm, free in solution, can be linked to a second affinity ligand. Binding of a target molecule to the two ligands brings the swing arm to AuNP and initiates autonomous, stepwise movement of the swing arm around the AuNP surface. The movement of the swing arm generates hundreds of oligonucleotides in response to a single binding event. The new nanomachines have several unique and advantageous features over DNA nanomachines that rely on DNA self-assembly.
    Type: Application
    Filed: September 12, 2016
    Publication date: March 16, 2017
    Inventors: Hongquan ZHANG, Xiaochun LE, Xing-Fang LI
  • Patent number: 9176123
    Abstract: This application relates to a binding-induced hairpin detection system comprising: a. a first probe comprising a targeting molecule and an oligonucleotide that has a free end and an end attached to the targeting molecule; and b. a second probe comprising a targeting molecule and an oligonucleotide that has an end attached to the targeting molecule and a free end comprising a nucleotide sequence that is complementary to a nucleotide sequence at or near the free end of the oligonucleotide of the first probe; wherein upon binding of the targeting molecule to a target molecule, the free end of the oligonucleotide of the second probe hybridizes at or near the free end of the oligonucleotide of the first probe forming a hairpin stem, the non-hybridized portions of the first and second probes together with the target molecule bound thereto forming a hairpin loop, thereby providing a binding-induced hairpin.
    Type: Grant
    Filed: August 30, 2010
    Date of Patent: November 3, 2015
    Assignee: THE GOVERNORS OF THE UNIVERSITY OF ALBERTA
    Inventors: Xiaochun Le, Hongquan Zhang, Xing-Fang Li
  • Patent number: 9150866
    Abstract: A method for single-cycle selection of aptamers is provided. More specifically, a method comprising single-cycle selection of aptamers for proteins blotted on a membrane is provided. In some embodiments, the present methods can comprise a deoxyribonuclease I (DNase I) mediated aptamers selection strategy that may be capable of isolating strong binding aptamers for target proteins from a crude protein extract. Aptamers selected using the present method are further provided. More specifically, the presently selected aptamers may be thermally stable, modifiable and easily produced through single-cycle synthesis process. The present aptamers may provide unique affinity reagents for use in diagnosing and detecting infectious disease (for example Hepatitis B), for research and biochemical studies (e.g. into molecular mechanisms). Further, the present aptamers may be utilized to develop unique assays or kits for clinical application, such as monitoring disease treatment and outcome.
    Type: Grant
    Filed: August 28, 2013
    Date of Patent: October 6, 2015
    Assignee: The Governors of the University of Alberta
    Inventors: Xing-Fang Li, X. Chris Le, Yanming Liu
  • Publication number: 20140148356
    Abstract: A method for single-cycle selection of aptamers is provided. More specifically, a method comprising single-cycle selection of aptamers for proteins blotted on a membrane is provided. In some embodiments, the present methods can comprise a deoxyribonuclease I (DNase I) mediated aptamers selection strategy that may be capable of isolating strong binding aptamers for target proteins from a crude protein extract. Aptamers selected using the present method are further provided. More specifically, the presently selected aptamers may be thermally stable, modifiable and easily produced through single-cycle synthesis process. The present aptamers may provide unique affinity reagents for use in diagnosing and detecting infectious disease (for example Hepatitis B), for research and biochemical studies (e.g. into molecular mechanisms). Further, the present aptamers may be utilized to develop unique assays or kits for clinical application, such as monitoring disease treatment and outcome.
    Type: Application
    Filed: August 28, 2013
    Publication date: May 29, 2014
    Applicant: The Governors of the University of Alberta
    Inventors: Xing-Fang Li, X. Chris Le, Yanming Liu
  • Patent number: 8507203
    Abstract: The invention is directed to aptamers, methods and kits comprising same for detecting Streptococcus pyogenes. The method of screening a subject for Streptococcus pyogenes involves obtaining a body sample from the subject; contacting the sample or a bacterial culture of the sample with an aptamer or a panel of aptamers specific to S. pyogenes; and detecting the presence or absence of S. pyogenes in the sample or the bacterial culture, wherein binding of the aptamer is indicative of the presence of S. pyogenes.
    Type: Grant
    Filed: March 29, 2012
    Date of Patent: August 13, 2013
    Assignee: The Governors of the University of Alberta
    Inventors: X. Chris Le, Camille Hamula, Xing-Fang Li
  • Publication number: 20120276547
    Abstract: The invention is directed to aptamers, methods and kits comprising same for detecting Streptococcus pyogenes. The method of screening a subject for Streptococcus pyogenes involves obtaining a body sample from the subject; contacting the sample or a bacterial culture of the sample with an aptamer or a panel of aptamers specific to S. pyogenes; and detecting the presence or absence of S. pyogenes in the sample or the bacterial culture, wherein binding of the aptamer is indicative of the presence of S. pyogenes.
    Type: Application
    Filed: March 29, 2012
    Publication date: November 1, 2012
    Applicant: GOVERNORS OR THE UNIVERSITY OF ALBERTA
    Inventors: X. Chris LE, Camille HAMULA, Xing-Fang LI
  • Publication number: 20120156678
    Abstract: This application relates to a binding-induced hairpin detection system comprising: a. a first probe comprising a targeting molecule and an oligonucleotide that has a free end and an end attached to the targeting molecule; and b. a second probe comprising a targeting molecule and an oligonucleotide that has an end attached to the targeting molecule and a free end comprising a nucleotide sequence that is complementary to a nucleotide sequence at or near the free end of the oligonucleotide of the first probe; wherein upon binding of the targeting molecule to a target molecule, the free end of the oligonucleotide of the second probe hybridizes at or near the free end of the oligonucleotide of the first probe forming a hairpin stem, the non-hybridized portions of the first and second probes together with the target molecule bound thereto forming a hairpin loop, thereby providing a binding-induced hairpin.
    Type: Application
    Filed: August 30, 2010
    Publication date: June 21, 2012
    Applicant: The Governors of the University of Alberta
    Inventors: Xiaochun Le, Hongquan Zhang, Xing-Fang Li