Abstract: Provided herein are methods and agents for gene specific demethylation and/or activation. Oligonucleotide constructs are provided, the oligonucleotide constructs including: [1] a targeting portion having sequence complementarity and binding affinity with a region of genomic DNA within a gene, near a gene, or both; and [2] a single guide RNA (sgRNA) scaffold portion, wherein a tetra-loop portion of the sgRNA is modified and includes an R2 stem loop of DNMT1-interacting RNA (DiR), and wherein a stem loop 2 portion of the sgRNA is modified and includes an R5 step loop of DiR. The oligonucleotide constructs may be used, together with deactivated (dead) Cas9 (dCas9) for providing gene specific demethylation and/or activation of gene(s) of interest in a cell or subject in need thereof.

Abstract: The invention provides a method and device for auto-generating GOOSE signal connection topology from substation level based on IEC61850 standard. The method comprises the following steps: import substation configuration language (SCL) file; search all GOOSE input and output signals under each access point; match the output signals to the input signals; and generate GOOSE signal connection topology based on the result of said matching. The device comprises an importing module for importing substation configuration language file; a GOOSE signal analyzer for searching all of GOOSE input and output signals under each access point, and matching said GOOSE output signals to said GOOSE input signals; and topology data module for generating GOOSE signal connection topology based on said matching. The device comprises a storage module. The storage module comprises an input dataset for storing GOOSE input signals and an output dataset for storing GOOSE output signals and their matching input signals.

Abstract: Methods of attaching high-temperature electrical components to substrates are provided. The methods involve of attachment of high-temperature components to substrates via a nano-metal film.

Abstract: A patterned molecular self-assembly is provided. The patterned molecular self-assembly comprises a support having an exposed patterned surface and a non-patterned surface. A compound is selectively adsorbed on the exposed patterned surface. The compound may comprise a first compound selectively adsorbed on the exposed patterned surface and a second compound selectively adsorbed on the first compound to form at least one bilayer. The patterned molecular self-assembly may further comprise a first set of bilayers and a second set of bilayers wherein the first set of bilayers has a different composition than the second set of bilayers.

Abstract: An electrostatic self-assembly method of fabricating electrostrictive and piezoelectric thin film assemblies not only provides a thinner film than is attainable by conventional methods, but provides excellent molecular-level uniformity and precise structural control, and thus large, effective piezoelectric coefficients. The method produces a thin film assembly including (a) a substrate, and (b) a film having one or a plurality of layers disposed upon the substrate, wherein at least one of the layers includes a dipolar material, and this layer of dipolar material has a uniform thickness of at most 500 nm.

Abstract: A pyrometer for measuring the temperature of an object with high accuracy and fast response time that uses both multi-wavelength pyrometry techniques and nanotechnology. Radiance from an object is transmitted through a fiber optic cable, is received by a thin-film multi-wavelength modulator, and is detected by an optical detector array. More specifically, the pyrometer includes means for conveying light; an optical lens; an optical fiber; means for optically modulating the wavelength of light; means for optically detecting the modulated light; means for transforming the optically detected wavelengths into electrical signals; and means for processing and recording the electrical signals. The means for optically modulating the wavelength includes at least one piezoelectric film deposited by an electrostatic self-assembly method.

Abstract: Abrasion and scratch protective coatings magnetic coatings, electrically and thermally conducting coatings, and UV absorbing coatings are provided by electrostatic self-assembly (ESA) of one layer of an organic or polymer molecule and one layer of inorganic clusters in a layer by layer fashion at room temperature. A combination of inorganic clusters having a particle size of preferably less than 30 nm and flexible organic molecules allows fabrication of films tens to hundreds of micrometers thick, with large pores and excellent stress relaxation.

Abstract: A patterned molecular self-assembly is provided. The patterned molecular self-assembly comprises a support having an exposed patterned surface and a non-patterned surface. A compound is selectively adsorbed on the exposed patterned surface. The compound may comprise a first compound selectively adsorbed on the exposed patterned surface and a second compound selectively adsorbed on the first compound to form at least one bilayer. The patterned molecular self-assembly may further comprise a first set of bilayers and a second set of bilayers wherein the first set of bilayers has a different composition than the second set of bilayers.