Abstract: The present disclosure discloses a method for automatically identifying south troughs by improved Laplace and relates to the technical field of meteorology. The method includes the following steps: acquiring grid data of a geopotential height field; calculating a gradient field of the geopotential height field in an x direction; searching for a turning point where a gradient value turned from being negative to being positive, and cleaning the gradient field; calculating a divergence of the x direction to obtain an improved Laplacian numerical value L?; performing 0,1 binarization processing on the L? to obtain a black-and-white image and a plurality of targets of potential troughs, merging the black-and-white image and the plurality of targets of the potential troughs by expansion, recovering original scale through erosion, and selecting an effective target through an angle of direction of a contour and an axial ratio.

Abstract: Provided is a multi-functional integrated molecular test cartridge and application thereof. The multi-functional integrated molecular test cartridge includes a deformable assembly, a blocking assembly and a reaction assembly, where the blocking assembly is placed between the deformable assembly and the reaction assembly, and a channel in communication with the reaction assembly and the deformable assembly is formed on the blocking assembly. The multi-functional integrated molecular test cartridge can be used for isothermal amplification reactions such as cross priming amplification (CPA), recombinase polymerase amplification (RPA), loop-mediated isothermal amplification (LAMP) and rolling circle amplification (RCA), can achieve integrated pre-loading of all reagents, a whole-process integrated test from a sample to a result, and flexible sample processing modes, and has high biological safety and a low risk of product and sample leakage.

Abstract: An nucleic acid integrated detection reagent tube is provided, it includes a main tube and one or more branch tubes, wherein a lysing zone, a cleaning zone and a plurality of separation plugs comprising at least a least a separation plug and a second separation plug which are sequentially disposed, the first separation plug is used for separating the lysing zone and the cleaning zone, a reaction zone is provided in the branch tubes and the second separation plug is positioned at a connection between the branch tubes and the main tube or inside the branch tubes; hydrophobic layers in a liquid or a solid phase disposed at each of the plurality of separation plugs; and a magnetic bead channel for magnetically carrying the nucleic acid to sequentially pass through each hydrophobic layer penetrating to the branch tubes defined in an inner wall of the detection reagent tube.

Abstract: An nucleic acid integrated detection method is provided, the method includes separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.

Abstract: A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.

Abstract: A biological sample processing device includes a containing bottle. A receiving opening is provided and a liquid outlet is provided. The device further includes an upper cover assembly and a lower cover. The upper cover assembly is connected to the upper end of the containing bottle. The lower cover is connected to the lower end of the containing bottle. The upper cover assembly includes an upper cover, a lower seal, a piston and a puncturing element. The upper cover is provided with a through hole, and the lower seal is disposed at a lower end of the through hole. The piston is closely matched with an inner wall of the through hole and can slide along the inner wall to drive the puncturing element to pierce the lower seal. The disclosure provides advantages that the biological sample can be processed by the provided device without opening the upper cover.

Abstract: A nucleic acid integrated detection method and detection reagent tube are provided, separating a lysis solution, a cleaning solution and a reaction solution in a detection reagent tube by providing a plurality of separation plugs in an over-under arrangement and disposing a hydrophobic layer in liquid or solid phase on each separation plug; adding a sample into the lysis solution; extracting nucleic acid in the sample using magnetic nanobeads; and then driving the magnetic nanobeads carrying the nucleic acid to sequentially pass through each hydrophobic layer along a magnetic bead channel and into the cleaning solution and the reaction solution to realize a cleaning and amplification for the nucleic acid, and finally, detecting the nucleic acid of the sample by an external device using an optical detection method, thus realizing a plurality of steps of nucleic acid extraction, cleaning and amplification reactions in the same detection reagent tube.