Abstract: The present invention provides a method for sequencing a protein/polypeptide based on Aerolysin nanopores to achieve specific discrimination of natural amino acids and post-translational modifications thereof and accurate acquisition of a sequence of a single-molecule protein, the method including the following steps: (1) unfolding of the protein; (2) terminus labeling of protein sequencing; (3) protein charge screening; (4) unfolding of a tertiary structure of the polypeptides; (5) orthogonal identification of amino acids; (6) confined perturbation-assisted identification of amino acids; and (7) single-molecule protein sequencing. The present invention aims at sensitive detection of sequence information about 20 amino acids and establishes an innovative method for accurately determining sequences of the amino acids and post-translational modifications of a single protein molecule.

Abstract: The present invention provides a nanopore single-molecule protein sequencer, which mainly includes a nanopore array chip system, an ultra-low current detection system and a data processing and construction system; the chip mainly includes a chip of peptide charge screening, a chip of amino acid sequence reading based on a series of specific nanopores and the like, the design principle of which is as follows: a series of biological nanopores with amino acid targeted identification are designed according to properties such as hydrophilicity and hydrophobicity, polarity, and chargeability of amino acids, and characteristic ion flow signals of amino acids forming a protein to be detected in nanopore are obtained one by one; characteristic information of a protein sequence in each of the nanopores is acquired by an arrayed ultra-low current measurement system; a standard model peptide sequence information base is used for identifying, correcting, integrating and reading amino acid sequences.

Abstract: The present invention provides a intelligent data center based on a service platform for vehicle-mounted devices, including: a static data storage unit, adapted for storing static data relating to a vehicle-mounted device; a static data processing unit, adapted for processing the static data stored in the static data storage unit; a dynamic data storage unit, adapted for storing dynamic data which are relating to the vehicle-mounted device and acquired by the service platform for vehicle-mounted devices; and a data analysis unit, adapted for analyzing the static data and the dynamic data. The intelligent data center can provide a wide variety of services for the vehicle-mounted device through the service platform.

Abstract: A vehicle information system includes: a vehicle-mounted device, a network processing device and a service center which is adapted for providing service for the vehicle-mounted device. A service configuration channel is configured between the network processing device and the service center and is adapted for transmitting configuration data which are adapted for configuring service that is provided for the vehicle-mounted device. The service configuration channel is established on basis of connection to internet. A service channel system is configured between the vehicle-mounted device and the service center. A complete solution for providing information service may be provided by the vehicle information system, thereby improving the quality of user experience while using a vehicle.

Abstract: A vehicle information system includes: a vehicle-mounted device, a voice service system and a service center which is adapted for providing service data. A first service channel system is configured between the voice service system and the vehicle-mounted device, and adapted for transmitting voice data. A second service channel is configured between the voice service system and the service center, and adapted for transmitting configuration data which is adapted for configuring the service data that are provided for the vehicle-mounted device. And the second service channel is established on basis of connection to internet. A third service channel system is configured between the service center and the vehicle-mounted device. A complete solution for providing real-time information service may be provided by the vehicle information system, thereby improving the quality of user experience while using a vehicle.