Abstract: A quantitative detection method of rare earth doped calcium phosphate fluorescent nanoparticles (RE-nCaP) in organisms includes establishing a fluorescent intensity-concentration standard curve of rare earth ions, preparing samples to be tested and the blank control group into homogenate, performing centrifuging and testing the fluorescent intensity of supernatants, calculating the fluorescent intensity values per unit mass or volume of the samples and the blank control group, and performing significant difference analysis; if P is greater than or equal to 0.05, determining that the RE-nCaP content in the samples is 0, and if P is smaller than 0.05, testing the tissue extraction rate of RE-nCaP; and comprehensively considering the tissue extraction rate, the homogenate volume, the fluorescent intensity value per until mass or volume, the homogenate dilution ratio, and the doping amount to obtain the accurate content of the RE-nCaP in biological tissue samples.

Abstract: The present invention relates to a polyvinyl alcohol/sodium alginate/hydroxyapatite composite fibrous membrane, and a preparation method and an application thereof. The preparation method of the composite fibrous membrane includes the following steps: firstly, reacting a diammonium hydrogen phosphate with a calcium nitrate to prepare a hydroxyapatite, and performing ultrasonic dispersion on the hydroxyapatite with a sodium alginate to form a stable hydroxyapatite suspension; separately preparing a sodium alginate solution of which the mass fraction is 2% and a polyvinyl alcohol solution of which the mass fraction is 18% using the above stable hydroxyapatite suspension; and finally, proportionally and uniformly mixing the two solutions, and performing electrospinning.

Abstract: The present invention relates to a polyvinyl alcohol/sodium alginate/hydroxyapatite composite fibrous membrane, and a preparation method and an application thereof. The preparation method of the composite fibrous membrane includes the following steps: firstly, reacting a diammonium hydrogen phosphate with a calcium nitrate to prepare a hydroxyapatite, and performing ultrasonic dispersion on the hydroxyapatite with a sodium alginate to form a stable hydroxyapatite suspension; separately preparing a sodium alginate solution of which the mass fraction is 2% and a polyvinyl alcohol solution of which the mass fraction is 18% using the above stable hydroxyapatite suspension; and finally, proportionally and uniformly mixing the two solutions, and performing electrospinning.

Abstract: A quantitative detection method of rare earth doped calcium phosphate fluorescent nanoparticles (RE-nCaP) in organisms includes establishing a fluorescent intensity-concentration standard curve of rare earth ions, preparing samples to be tested and the blank control group into homogenate, performing centrifuging and testing the fluorescent intensity of supernatants, calculating the fluorescent intensity values per unit mass or volume of the samples and the blank control group, and performing significant difference analysis; if P is greater than or equal to 0.05, determining that the RE-nCaP content in the samples is 0, and if P is smaller than 0.05, testing the tissue extraction rate of RE-nCaP; and comprehensively considering the tissue extraction rate, the homogenate volume, the fluorescent intensity value per until mass or volume, the homogenate dilution ratio, and the doping amount to obtain the accurate content of the RE-nCaP in biological tissue samples.

Abstract: An RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/?-tricalcium phosphate composite material is composed of ?-tricalcium phosphate particles and RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) with mass ratio of 1:10-1:100, in which the ?-tricalcium phosphate particles are uniformly dispersed in the RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) matrix. The preparation method includes that poly (glycolic acid-L-lysine-L-lactic acid) is polymerized with GRGDY short peptide (glycin-arginine-glycin-aspartic acid-tyrosine sequence) to obtain RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid), and then RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) is compounded with ?-tricalcium phosphate particles.

Abstract: An RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid)/?-tricalcium phosphate composite material is composed of ?-tricalcium phosphate particles and RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) with mass ratio of 1:10-1:100, in which the ?-tricalcium phosphate particles are uniformly dispersed in the RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) matrix. The preparation method includes that poly (glycolic acid-L-lysine-L-lactic acid) is polymerized with GRGDY short peptide (glycin-arginine-glycin-aspartic acid-tyrosine sequence) to obtain RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid), and then RGD polypeptide grafted poly (glycolic acid-L-lysine-L-lactic acid) is compounded with ?-tricalcium phosphate particles.