Patents by Inventor Yoshiaki Nishiya
Yoshiaki Nishiya has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9133446Abstract: The present invention provides AP that has a high specific activity and preferably has superior reactivity with respect to various luminescent substrates generally used for high-sensitivity immunoassay analysis. Further preferably, the present invention provides AP having a thermal stability higher than that of CIAP. The alkaline phosphatase of the present invention is derived from the genus Shewanella and has the following characteristics: (A) molecular weight: about 104,000; (B) optimum reaction pH: about 9.5; (C) stable pH range: 5.5 to 10.4; (D) thermal stability: 65° C., and (E) specific activity: 5,000 U/mg or more.Type: GrantFiled: August 22, 2013Date of Patent: September 15, 2015Assignee: TOYOBO CO., LTD.Inventors: Hiroshi Aiba, Takahide Kishimoto, Yoshiaki Nishiya
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Patent number: 8993255Abstract: The present invention provides: a protein having a fructosyl amino acid oxidase activity which protein is useful for measurement of a glycosylated protein (particularly, glycosylated hemoglobin); a modified protein thereof; and use of the protein or the modified protein. The protein of the present invention is, for example, a fructosyl valyl histidine oxidase derived from Phaeosphaeria nodorum, the fructosyl valyl histidine oxidase having excellent thermal stability and substrate specificity and also having a small Km value to fructosyl valyl histidine. This allows a glycosylated protein measuring reagent to be stored in a long time and measurement accuracy of the glycosylated protein measuring reagent to be improved.Type: GrantFiled: October 6, 2009Date of Patent: March 31, 2015Assignees: Toyo Boseki Kabushiki Kaisha, Gekkeikan Sake Co., Ltd.Inventors: Rie Hirao, Masao Kitabayashi, Yoshiaki Nishiya, Hiroki Ishida, Yoji Hata
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Patent number: 8728771Abstract: The present invention provides a L-succinylacylase consisting of: (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; or (d) a protein which consists of an amino acid sequence where one or several amino acid(s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity. This enzyme is able to produce a sterically bulky unnatural amino acid such as L-tert-leucine etc. which is useful as an intermediate for pharmaceuticals.Type: GrantFiled: December 10, 2009Date of Patent: May 20, 2014Assignees: Toyo Boseki Kabushiki Kaisha, Sekisui Medical Co., Ltd.Inventors: Atsushi Toda, Sachio Iwai, Yoshiaki Nishiya, Shinya Kumagai
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Publication number: 20140030790Abstract: The present invention provides AP that has a high specific activity and preferably has superior reactivity with respect to various luminescent substrates generally used for high-sensitivity immunoassay analysis. Further preferably, the present invention provides AP having a thermal stability higher than that of CIAP. The alkaline phosphatase of the present invention is derived from the genus Shewanella and has the characteristics below: (A) molecular weight: about 104,000; (B) optimum reaction pH: about 9.5; (C) stable pH range: 5.5 to 10.4; (D) thermal stability: 65° C.; and (E) specific activity: 5,000 U/mg or more.Type: ApplicationFiled: August 22, 2013Publication date: January 30, 2014Applicant: TOYOBO CO., LTD.Inventors: Hiroshi Aiba, Takahide Kishimoto, Yoshiaki Nishiya
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Patent number: 8470574Abstract: The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals. A protein which is characterized in being represented by any of the following (a) to (d): (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; and (d) a protein which consists of an amino acid sequence where one or several amino acid (s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity.Type: GrantFiled: May 7, 2009Date of Patent: June 25, 2013Assignees: Toyo Boseki Kabushiki Kaisha, Sekisui Medical Co., Ltd.Inventors: Atsushi Toda, Sachio Iwai, Yoshiaki Nishiya, Takeshi Miyata, Aya Oosato, Shinya Kumagai, Toshihide Yamada
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Patent number: 8394615Abstract: The invention provides a glucose dehydrogenase that is an extremely stable enzyme having a thermostability of 80° C. or more, and that does not substantially act upon saccharides other than glucose (e.g., having a reactivity of less than 3% with respect to maltose, galactose, and xylose). The invention also provides a method for producing such an enzyme, and a composition for quantifying glucose using such an enzyme.Type: GrantFiled: December 26, 2008Date of Patent: March 12, 2013Assignees: Toyo Boseki Kabushiki Kaisha, Kyoto UniversityInventors: Hiroshi Aiba, Yoshiaki Nishiya, Tadayuki Imanaka, Haruyuki Atomi
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Patent number: 8247189Abstract: Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO:2 or an amino acid sequence that has 60% homology therewith, and that has an improved temperature dependency.Type: GrantFiled: March 15, 2012Date of Patent: August 21, 2012Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroshi Kawaminami, Masao Kitabayashi, Yoshiaki Nishiya
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Publication number: 20120171708Abstract: Provided is an enzyme that is further advantageous in terms of practical aspects when compared to publicly known enzymes for blood sugar sensors, and that can be used in a blood sugar level measuring reagent. A flavin adenine dinucleotide-dependent glucose dehydrogenase that has amino acid sequence including a specific amino acid in an amino acid sequence shown in SEQ ID NO: 2 or an amino acid sequence that has a 60% homology therewith, and that has an improved temperature dependency.Type: ApplicationFiled: March 15, 2012Publication date: July 5, 2012Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Hiroshi KAWAMINAMI, Masao KITABAYASHI, Yoshiaki NISHIYA
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Patent number: 8039248Abstract: The invention provides a modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH), as well as a glucose sensor comprising the modified FADGDH and a method for measuring glucose comprising using the glucose sensor to measure glucose of a sample.Type: GrantFiled: August 25, 2009Date of Patent: October 18, 2011Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroshi Kawaminami, Yuji Tsuji, Masao Kitabayashi, Yoshiaki Nishiya
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Publication number: 20110250653Abstract: The present invention provides a L-succinylacylase consisting of: (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; or (d) a protein which consists of an amino acid sequence where one or several amino acid(s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity. This enzyme is able to produce a sterically bulky unnatural amino acid such as L-tert-leucine etc. which is useful as an intermediate for pharmaceuticals.Type: ApplicationFiled: December 10, 2009Publication date: October 13, 2011Applicants: SEKISUI MEDICAL CO., LTD., TOYO BOSEKI KABUSHIKI KAISHAInventors: Atsushi Toda, Sachio Iwai, Yoshiaki Nishiya, Shinya Kumagai
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Publication number: 20110244530Abstract: The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals. A protein which is characterized in being represented by any of the following (a) to (d): (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; and (d) a protein which consists of an amino acid sequence where one or several amino acid (s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity.Type: ApplicationFiled: May 7, 2009Publication date: October 6, 2011Applicants: TOYO BOSEKI KABUSHIKI KAISHA, SEKISUI MEDICAL CO., LTD.Inventors: Atsushi Toda, Sachio Iwai, Yoshiaki Nishiya, Takeshi Miyata, Aya Oosato, Shinya Kumagai, Toshihide Yamada
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Publication number: 20110195444Abstract: The present invention provides: a protein having a fructosyl amino acid oxidase activity which protein is useful for measurement of a glycosylated protein (particularly, glycosylated hemoglobin); a modified protein thereof; and use of the protein or the modified protein. The protein of the present invention is, for example, a fructosyl valyl histidine oxidase derived from Phaeosphaeria nodorum, the fructosyl valyl histidine oxidase having excellent thermal stability and substrate specificity and also having a small Km value to fructosyl valyl histidine. This allows a glycosylated protein measuring reagent to be stored in a long time and measurement accuracy of the glycosylated protein measuring reagent to be improved.Type: ApplicationFiled: October 6, 2009Publication date: August 11, 2011Applicants: TOYO BOSEKI KABUSHIKI KAISHA, GEKKEIKAN SAKE CO., LTD.Inventors: Rie Hirao, Masao Kitabayashi, Yoshiaki Nishiya, Hiroki Ishida, Yoji Hata
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Publication number: 20110020851Abstract: The invention provides a glucose dehydrogenase that is an extremely stable enzyme having a thermostability of 80° C. or more, and that does not substantially act upon saccharides other than glucose (e.g., having a reactivity of less than 3% with respect to maltose, galactose, and xylose). The invention also provides a method for producing such an enzyme, and a composition for quantifying glucose using such an enzyme.Type: ApplicationFiled: December 26, 2008Publication date: January 27, 2011Applicants: TOYO BOSEKI KABUSHIKI KAISHA, KYOTO UNIVERSITYInventors: Hiroshi Aiba, Yoshiaki Nishiya, Tadayuki Imanaka, Haruyuki Atomi
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Patent number: 7871805Abstract: The present invention provides glucose dehydrogenase which is excellent in heat resistance and substrate specificity and is not affected by dissolved oxygen. Specifically, the present invention relates to glucose dehydrogenase characterized by being derived from an eukaryotic organism and keeping 90% or more activity after being treated with heat at 55° C. for 15 minutes in a liquid form compared with the activity before being treated.Type: GrantFiled: January 9, 2009Date of Patent: January 18, 2011Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroshi Aiba, Hiroshi Kawaminami, Takahide Kishimoto, Yoshiaki Nishiya
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Patent number: 7816116Abstract: To overcome disadvantages of a known creatinine amide hydrolase, and provide a creatinine amide hydrolase having improved affinity for creatinine or having a decreased Km value for creatinine, and also provide a reagent composition for use in the determination of creatinine, which is adapted to an automated analysis apparatus and is excellent in accuracy, preciseness and economic efficiency. Disclosed is a modified creatinine amide hydrolase having improved affinity for a substrate compared to an unmodified one. Also disclosed is a creatinine determination reagent comprising the modified creatinine amide hydrolase, a creatinine amidino hydrolase, sarcosine oxidase and a reagent for detection of hydrogen peroxide.Type: GrantFiled: April 20, 2007Date of Patent: October 19, 2010Assignee: Toyo Boseki Kabushiki KaishaInventors: Rie Nagai, Masao Kitabayashi, Yoshiaki Nishiya
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Patent number: 7741100Abstract: The present invention provides a method for highly expressing a recombinant FAD-GDH protein derived from filamentous fungi, protein obtained by the method, and a regent for measuring glucose using the protein. According to the invention, the FAD-GDH can be highly expressed by altering DNA sequence coding for a signal peptide of FAD-GDH gene isolated from Aspergillus oryzae. FAD-GDH can be stably produced by adjusting pH of 7.1 to 7.3 during culture production.Type: GrantFiled: March 28, 2007Date of Patent: June 22, 2010Assignee: Toyo Boseki Kabushiki KaishaInventors: Masao Kitabayashi, Yuji Tsuji, Hiroshi Kawaminami, Takahide Kishimoto, Yoshiaki Nishiya
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Patent number: 7662600Abstract: An object of the present invention is to provide a more practically advantageous enzyme usable as a reagent for measuring blood glucose than the known enzymes used as blood glucose sensors. A modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH) with more improved heat stability than FADGDH derived from wild-type FADGDH, the modified FADGDH being derived from preferably a eukaryote, more preferably a filamentous fungus, and furthermore preferably an Aspergillus fungus, and, for example, those having a primary structure with at least one amino acid substituted, deleted, inserted or added to FADGDH having an amino acid sequence represented by SEQ ID Nos. 2 or 46 in the sequence table.Type: GrantFiled: November 13, 2007Date of Patent: February 16, 2010Assignee: Toyo Boseki Kabushiki KaishaInventors: Hiroshi Kawaminami, Yuji Tsuji, Masao Kitabayashi, Yoshiaki Nishiya
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Patent number: 7655130Abstract: The present invention effectively produces glucose dehydrogenase derived from Aspergillus oryzae, and provides more practical glucose dehydrogenase. The invention makes it possible to efficiently produce glucose dehydrogenase and to obtain glucose dehydrogenase in more practical manner by using a glucose dehydrogenase gene isolated from Aspergillus oryzae.Type: GrantFiled: May 18, 2009Date of Patent: February 2, 2010Assignee: Toyo Boseki Kabushiki KaishaInventors: Yuji Tsuji, Masao Kitabayashi, Takahide Kishimoto, Yoshiaki Nishiya
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Publication number: 20090317848Abstract: The invention provides a modified flavin adenine dinucleotide dependent glucose dehydrogenase (FADGDH), as well as a glucose sensor comprising the modified FADGDH and a method for measuring glucose comprising using the glucose sensor to measure glucose of a sampleType: ApplicationFiled: August 25, 2009Publication date: December 24, 2009Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Hiroshi KAWAMINAMI, Yuji TSUJI, Masao KITABAYASHI, Yoshiaki NISHIYA
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Publication number: 20090259024Abstract: The present invention effectively produces glucose dehydrogenase derived from Aspergillus oryzae, and provides more practical glucose dehydrogenase. The invention makes it possible to efficiently produce glucose dehydrogenase and to obtain glucose dehydrogenase in more practical manner by using a glucose dehydrogenase gene isolated from Aspergillus oryzae.Type: ApplicationFiled: May 18, 2009Publication date: October 15, 2009Applicant: TOYO BOSEKI KABUSHIKI KAISHAInventors: Yuji TSUJI, Masao KITABAYASHI, Takahide KISHIMOTO, Yoshiaki NISHIYA