Patents by Inventor Yoshiharu Asaoka

Yoshiharu Asaoka has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11542301
    Abstract: The present application addresses the problem of providing an Fc-binding protein having an improved antibody separation ability. The present application also addresses the problem of providing a high-accuracy antibody separation method using an insoluble carrier having the protein immobilized thereon. The problems can be solved by: an Fc-binding protein in which at least an amino acid substitution at a specific position therein occurs and which has reduced affinity for an antibody; and an antibody separation method including allowing an equilibration buffer solution to pass through a column in which an insoluble carrier having the protein immobilized thereon is filled to equilibrate the column, adding a solution containing an antibody to cause the adsorption of the antibody onto the carrier, and eluting the antibody adsorbed on the carrier using an elution solution.
    Type: Grant
    Filed: February 7, 2018
    Date of Patent: January 3, 2023
    Assignee: TOSOH CORPORATION
    Inventors: Yosuke Terao, Yoshiharu Asaoka, Ryoko Otake, Satoshi Endo, Naoki Yamanaka, Yukie Yamamoto, Seigo Oe
  • Publication number: 20220402995
    Abstract: The present invention provides an improved adeno-associated virus (AAV)-binding protein having enhanced stability, especially, heat stability, acid stability and alkali stability, of an AAV-binding protein, a method for producing the improved AAV-binding protein, and an AAV adsorbent using the same.
    Type: Application
    Filed: November 24, 2020
    Publication date: December 22, 2022
    Applicant: TOSOH CORPORATION
    Inventors: Yoshiharu ASAOKA, Hidetaka KOBAYASHI, Yuriko MAKINO, Kouhei YOSHIDA, Kento KURIHARA
  • Patent number: 10815289
    Abstract: Provided are: an Fc-binding protein having improved stability, particularly to heat and acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent. Specifically provided are: an Fc-binding protein having improved stability to heat and acid, achieved by substituting an amino-acid residue in a specific position in the extracellular region of human FcyRIIIa with another specific amino acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent.
    Type: Grant
    Filed: June 24, 2015
    Date of Patent: October 27, 2020
    Assignee: TOSOH CORPORATION
    Inventors: Yoshiharu Asaoka, Toru Tanaka, Yosuke Terao, Naoki Yamanaka, Natsuko Kizu, Masaru Aoki, Teruhiko Ide
  • Publication number: 20200223885
    Abstract: The present application addresses the problem of providing an Fc-binding protein having an improved antibody separation ability. The present application also addresses the problem of providing a high-accuracy antibody separation method using an insoluble carrier having the protein immobilized thereon. The problems can be solved by: an Fc-binding protein in which at least an amino acid substitution at a specific position therein occurs and which has reduced affinity for an antibody; and an antibody separation method including allowing an equilibration buffer solution to pass through a column in which an insoluble carrier having the protein immobilized thereon is filled to equilibrate the column, adding a solution containing an antibody to cause the adsorption of the antibody onto the carrier, and eluting the antibody adsorbed on the carrier using an elution solution.
    Type: Application
    Filed: February 7, 2018
    Publication date: July 16, 2020
    Applicant: TOSOH CORPORATION
    Inventors: Yosuke TERAO, Yoshiharu ASAOKA, Ryoko OTAKE, Satoshi ENDO, Naoki YAMANAKA, Yukie YAMAMOTO, Seigo OE
  • Patent number: 10611817
    Abstract: The present invention addresses the first problem of providing an Fc-binding protein having improved stability, especially stability to heat and acid, of the Fc-binding protein, a method for producing this protein, and an antibody adsorbent using this protein. The present invention also addresses the second problem of providing a method that makes it possible to identify the presence or absence of glycosylation of an antibody, and a material to be used in this method. The first problem is solved by an Fc-binding protein having improved stability to heat and acid obtained by substituting amino acid residues at specific positions in the extracellular domain within human Fc?RIIIa with other specific amino acids, a method for producing this protein, and an antibody adsorbent using this protein. The second problem is solved by using an adsorbent capable of specifically adsorbing an antibody having a sugar chain, the adsorbent being obtained by immobilizing human Fc?RIIIa on an insoluble carrier.
    Type: Grant
    Filed: September 18, 2014
    Date of Patent: April 7, 2020
    Assignee: TOSOH CORPORATION
    Inventors: Yoshiharu Asaoka, Toru Tanaka, Teruhiko Ide
  • Publication number: 20190211077
    Abstract: Provided are: an Fc-binding protein having improved stability, particularly to heat and acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent. Specifically provided are: an Fc-binding protein having improved stability to heat and acid, achieved by substituting an amino-acid residue in a specific position in the extracellular region of human FcyRIIIa with another specific amino acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent.
    Type: Application
    Filed: June 24, 2015
    Publication date: July 11, 2019
    Applicant: TOSOH CORPORATION
    Inventors: Yoshiharu ASAOKA, Toru TANAKA, Yosuke TERAO, Naoki YAMANAKA, Natsuko KIZU, Masaru AOKI, Teruhiko IDE
  • Publication number: 20170218044
    Abstract: Provided are: an Fc-binding protein having improved stability, particularly to heat and acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent. Specifically provided are: an Fc-binding protein having improved stability to heat and acid, achieved by substituting an amino-acid residue in a specific position in the extracellular region of human FcyRIIIa with another specific amino acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent.
    Type: Application
    Filed: June 24, 2015
    Publication date: August 3, 2017
    Applicant: TOSOH CORPORATION
    Inventors: Yoshiharu ASAOKA, Toru TANAKA, Yosuke TERAO, Naoki YAMANAKA, Natsuko KIZU, Masaru AOKI, Teruhiko IDE
  • Patent number: 9453066
    Abstract: Disclosed are: an Fc binding protein having increased stability with respect to heat, acid, and/or alkalinity compared with the wild type; a method for producing same; and a method for specifically isolating protein containing an Fc binding protein binding site using said Fc binding protein as a ligand for affinity chromatography. An Fc binding protein was obtained having increased stability with respect to heat, acid, and/or alkalinity compared with the wild-type human Fc receptor by means of substituting at least one specific amino acid residue in the extracellular domain of the wild-type human Fc receptor with another amino acid residue. The Fc binding protein is useful as a ligand for affinity chromatography for example by immobilizing in a solid phase.
    Type: Grant
    Filed: March 10, 2011
    Date of Patent: September 27, 2016
    Assignees: Sagami Chemical Research Institute, TOSOH CORPORATION
    Inventors: Kouta Hatayama, Yoshiharu Asaoka, Toru Tanaka, Teruhiko Ide
  • Publication number: 20160222081
    Abstract: The present invention addresses the first problem of providing an Fc-binding protein having improved stability, especially stability to heat and acid, of the Fc-binding protein, a method for producing this protein, and an antibody adsorbent using this protein. The present invention also addresses the second problem of providing a method that makes it possible to identify the presence or absence of glycosylation of an antibody, and a material to be used in this method. The first problem is solved by an Fc-binding protein having improved stability to heat and acid obtained by substituting amino acid residues at specific positions in the extracellular domain within human Fc?RIIIa with other specific amino acids, a method for producing this protein, and an antibody adsorbent using this protein. The second problem is solved by using an adsorbent capable of specifically adsorbing an antibody having a sugar chain, the adsorbent being obtained by immobilizing human Fc?RIIIa on an insoluble carrier.
    Type: Application
    Filed: September 18, 2014
    Publication date: August 4, 2016
    Applicant: TOSOH CORPORATION
    Inventors: Yoshiharu ASAOKA, Toru TANAKA, Teruhiko IDE
  • Publication number: 20130079499
    Abstract: Disclosed are: an Fc binding protein having increased stability with respect to heat, acid, and/or alkalinity compared with the wild type; a method for producing same; and a method for specifically isolating protein containing an Fc binding protein binding site using said Fc binding protein as a ligand for affinity chromatography. An Fc binding protein was obtained having increased stability with respect to heat, acid, and/or alkalinity compared with the wild-type human Fc receptor by means of substituting at least one specific amino acid residue in the extracellular domain of the wild-type human Fc receptor with another amino acid residue. The Fc binding protein is useful as a ligand for affinity chromatography for example by immobilizing in a solid phase.
    Type: Application
    Filed: March 10, 2011
    Publication date: March 28, 2013
    Applicants: TOSOH CORPORATION, SAGAMI CHEMICAL RESEARCH INSTITUTE
    Inventors: Kouta Hatayama, Yoshiharu Asaoka, Toru Tanaka, Teruhiko Ide
  • Publication number: 20020037752
    Abstract: A mobile radio communication apparatus includes a liquid crystal display section configured to display information, keys configured to input information, a first illuminating section configured to illuminate the liquid crystal display section, and a second illuminating section configured to illuminate the keys. The apparatus further includes a detecting section configured to detect a predetermined operation of the apparatus, and a control section configured to operate the first illuminating section to illuminate the liquid crystal display section and not to operate the second illuminating section so as not to illuminate the keys, when the detecting section detects the predetermined operation of the apparatus. This makes it possible to provide a mobile radio communication apparatus capable of reducing the current consumption and permitting a user to reliably check information displayed on the LCD screen.
    Type: Application
    Filed: September 20, 2001
    Publication date: March 28, 2002
    Inventor: Yoshiharu Asaoka
  • Patent number: 5878340
    Abstract: When a vehicle radio telephone has transmitted a call signal, a base-station/control-station suspends the communication channel. After the base-station/control-station transmits a PIN transmission request signal to the vehicle radio telephone via the communication channel, a liquid crystal display 452 incorporated in the telephone displays a PIN transmission request. Operating a key section 430 in the telephone generates a flash operation signal. When the base-station/control-station has received the flash operation signal, it transmits a telephone number transmission request signal. At this time, the PIN transmission request on the display 452 is erased, and a PIN is read from an ID/ROM and a PIN code signal is transmitted. Upon receiving the PIN code signal, the base-station/control-station examines whether or not the PIN indicated by the PIN code signal identical to a registered one.
    Type: Grant
    Filed: January 24, 1996
    Date of Patent: March 2, 1999
    Assignee: Kabushiki Kaisha Toshiba
    Inventors: Yoshiharu Asaoka, Shinya Takahashi, Takashi Sakagawa