Patents by Inventor Yoshihiko Yasohara

Yoshihiko Yasohara has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20130183716
    Abstract: The invention relates to a glucose dehydrogenase showing an NADP/NAD activity ratio, namely the value obtained by dividing the enzyme activity value obtained by using NADP as a coenzyme by the enzyme activity value obtained by using NAD as a coenzyme, of not lower than 300, to a gene coding therefor, and to a transformant harboring that gene. The enzyme of the invention is very high in NADP specificity and can be suitably used, for example, for NADPH production, for coenzyme regeneration in enzymatic reduction reactions, and in biosensors for glucose concentration measurements.
    Type: Application
    Filed: September 24, 2008
    Publication date: July 18, 2013
    Applicant: Kaneka Corporation
    Inventors: Shigeru Kawano, Yoshihiko Yasohara
  • Publication number: 20130149769
    Abstract: The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to product (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.
    Type: Application
    Filed: April 25, 2011
    Publication date: June 13, 2013
    Applicant: KANEKA CORPORATION
    Inventors: Noriyuki KIZAKI, Makoto UEDA, Daisuke MORIYAMA, Naoaki TAOKA, Yoshihiko YASOHARA
  • Patent number: 8431378
    Abstract: The invention relates to a novel aminotransferase, DNA encoding the enzyme, a recombinant vector into which the DNA has been introduced, and a transformant into which the vector has been introduced. Further, the invention also relates to a method for producing an optically active amino compound utilizing the enzyme or transformant. The aminotransferase of the invention has an ability of efficiently converting a ketone compound, particularly a cyclic ketone compound to an optically active amino compound. According to the invention, a method for efficiently producing an optically active amino compound, particularly an optically active cyclic amino compound is provided.
    Type: Grant
    Filed: January 27, 2012
    Date of Patent: April 30, 2013
    Assignee: Kaneka Corporation
    Inventors: Noriyuki Ito, Yoshihiko Yasohara
  • Patent number: 8304216
    Abstract: The present invention has its object to provide a method of producing an erythro- or threo-2-amino-3-hydroxypropionic acid ester, and so forth. The present invention relates to: a method of asymmetrically reducing an N-2-amino-3-oxopropionic acid ester by allowing cells of a microorganism to act thereon; a polypeptide having an activity of asymmetrically reducing a carbonyl compound to give an optically active alcohol, which is isolated from a microorganism belonging to genus Brevundimonas; a DNA coding for the polypeptide; and a transformant producing the polypeptide. The invention also relates to a method of producing an optically active alcohol by reducing a carbonyl compound with the help of the polypeptide or the transformant.
    Type: Grant
    Filed: March 29, 2007
    Date of Patent: November 6, 2012
    Assignee: Kaneka Corporation
    Inventors: Tozo Nishiyama, Yoshihiko Yasohara
  • Publication number: 20120164724
    Abstract: The invention relates to a novel aminotransferase, DNA encoding the enzyme, a recombinant vector into which the DNA has been introduced, and a transformant into which the vector has been introduced. Further, the invention also relates to a method for producing an optically active amino compound utilizing the enzyme or transformant. The aminotransferase of the invention has an ability of efficiently converting a ketone compound, particularly a cyclic ketone compound to an optically active amino compound. According to the invention, a method for efficiently producing an optically active amino compound, particularly an optically active cyclic amino compound is provided.
    Type: Application
    Filed: January 27, 2012
    Publication date: June 28, 2012
    Inventors: NORIYUKI ITO, YOSHIHIKO YASOHARA
  • Patent number: 8168412
    Abstract: The present invention relates to a method for producing an optically-active amine compound. The method is characterized by using a transaminase (A), an ?-keto acid reductase (B), and an enzyme (C), each having specific properties, in an identical reaction system to convert a ketone compound into a corresponding optically-active amine compound in which a carbon atom with an amino group bonded thereto serves as an asymmetric point. The present invention also relates to a recombinant vector for use in the method. The present invention makes it possible to efficiently produce an optically-active amine compound.
    Type: Grant
    Filed: May 28, 2007
    Date of Patent: May 1, 2012
    Assignee: Kaneka Corporation
    Inventors: Shigeru Kawano, Noriyuki Ito, Yoshihiko Yasohara
  • Patent number: 8133705
    Abstract: The invention relates to a novel aminotransferase, DNA encoding the enzyme, a recombinant vector into which the DNA has been introduced, and a transformant into which the vector has been introduced. Further, the invention also relates to a method for producing an optically active amino compound utilizing the enzyme or transformant. The aminotransferase of the invention has an ability of efficiently converting a ketone compound, particularly a cyclic ketone compound to an optically active amino compound. According to the invention, a method for efficiently producing an optically active amino compound, particularly an optically active cyclic amino compound is provided.
    Type: Grant
    Filed: May 22, 2006
    Date of Patent: March 13, 2012
    Assignee: Kaneka Corporation
    Inventors: Noriyuki Ito, Yoshihiko Yasohara
  • Patent number: 8129163
    Abstract: An object of the present invention is to provide a novel alcohol dehydrogenase, a gene for the alcohol dehydrogenase, a vector including the gene, a transformant transformed with the vector, and a method for producing an optically active alcohol by utilizing them. A feature of the present invention directs to a novel polypeptide isolated from Candida maltosa, a DNA coding for the polypeptide, and a transformant producing the polypeptide. Another feature of the present invention directs to a method for producing an optically-active alcohol by reducing a carbonyl compound with the polypeptide or the transformant.
    Type: Grant
    Filed: November 27, 2007
    Date of Patent: March 6, 2012
    Assignee: Kaneka Corporation
    Inventors: Shigeru Kawano, Takeru Ishige, Keita Iguchi, Tozo Nishiyama, Yoshihiko Yasohara
  • Publication number: 20110281309
    Abstract: The present invention relates to a novel amino acid dehydrogenase, DNA encoding the enzyme, and a transformant having the DNA introduced therein. The present invention also relates to a process for producing an L-amino acid, 2-oxo acid or D-amino acid, which includes allowing the amino acid dehydrogenase or a microorganism or transformant capable of producing the enzyme to act on a substrate compound. The amino acid dehydrogenase has good reactivity even with an amino acid or a 2-oxo acid each having a bulky side chain such as an aromatic-ring-containing group, which acids are poorly reactive with conventional amino acid dehydrogenases. The amino acid dehydrogenase enables the inexpensive and highly efficient production of a useful optically active amino acid.
    Type: Application
    Filed: December 8, 2009
    Publication date: November 17, 2011
    Applicant: KANEKA CORPORATION
    Inventors: Hiroyuki Kanamaru, Ryuuji Miki, Makoto Ueda, Hironori Nanba, Yoshihiko Yasohara
  • Publication number: 20110236955
    Abstract: The present invention provides an efficient method of producing an arylacylamidase. In an aspect, the present invention provides (a) a DNA comprising the base sequence shown in the sequence listing under SEQ ID NO:1, or (b) a DNA capable of hybridizing, under stringent conditions, with a DNA comprising the base sequence complementary to the base sequence shown in the sequence listing under SEQ ID NO:1 and coding for a polypeptide having arylacylamidase activity. In another aspect, the invention provides a polypeptide encoded by the DNA mentioned above and having arylacylamidase activity, a vector containing the DNA mentioned above, a transformant which is transformed using the DNA or the vector mentioned above, and a method for producing an arylacylamidase which comprises cultivating the transformant mentioned above and obtaining the arylacylamidase from the culture medium.
    Type: Application
    Filed: September 26, 2005
    Publication date: September 29, 2011
    Applicant: KANEKA CORPORATION
    Inventors: Noriyuki Kizaki, Noriyuki Ito, Yoshihiko Yasohara, Toru Nagasawa
  • Publication number: 20110237531
    Abstract: The present invention provides a low-cost, efficient method for producing a glycolipid biosurfactant, in particular, lactonic sophorose lipids. This method is characterized by culturing a microorganism capable of producing the biosurfactant under limited oxygen supply. The present invention enables preferential production of lactonic sophorose lipids and facilitates recovery of the lactonic sophorose lipids in a solid form. Further, the present invention enables production of high purity acidic sophorose lipids by hydrolyzing high purity lactonic sophorose lipids produced by the above method. The present invention also provides lactonic sophorose lipids that possess strong antibacterial and antifungal activities, and an antibacterial and/or antifungal agent containing the sophorose lipids.
    Type: Application
    Filed: October 23, 2009
    Publication date: September 29, 2011
    Applicant: KANEKA CORPORATION
    Inventors: Satohiro Yanagisawa, Shigeru Kawano, Yoshihiko Yasohara
  • Patent number: 8008461
    Abstract: The present invention is to provide a process for efficiently producing an optically active alcohol including (R)-3-hydroxy-3-phenylpropanenitrile. One of the features of the present invention is a polypeptide having an activity of asymmetrically reducing 3-oxo-3-phenylpropanenitrile isolated from a microorganism belonging to the genus Candida to produce (R)-3-hydroxy-3-phenylpropanenitrile, DNA encoding the polypeptide and a transformant of producing the polypeptide. Another feature of the present invention is a process for producing an optically active alcohol such as (R)-3-hydroxy-3-phenylpropanenitrile by reducing a carbonyl compound such as 3-oxo-3-phenylpropanenitrile by use of the polypeptide or the transformant.
    Type: Grant
    Filed: October 20, 2005
    Date of Patent: August 30, 2011
    Assignee: Kaneka Corporation
    Inventors: Noriyuki Kizaki, Makoto Ueda, Daisuke Moriyama, Naoaki Taoka, Yoshihiko Yasohara
  • Patent number: 7915022
    Abstract: The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant. Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.
    Type: Grant
    Filed: July 20, 2010
    Date of Patent: March 29, 2011
    Assignee: Kaneka Corporation
    Inventors: Noriyuki Kizaki, Miho Yano, Masashiro Funaki, Teruaki Takesue, Yoshihiko Yasohara, Souichi Morikawa, Takahisa Nakai, Michihiko Kataoka, Sakayu Shimizu
  • Publication number: 20100317075
    Abstract: The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant. Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.
    Type: Application
    Filed: July 20, 2010
    Publication date: December 16, 2010
    Applicant: KANEKA CORPORATION
    Inventors: NORIYUKI KIZAKI, MIHO YANO, MASAHIRO FUNAKI, TERUAKI TAKESUE, YOSHIHIKO YASOHARA, SOUICHI MORIKAWA, TAKAHISA NAKAI, MICHIHIKO KATAOKA, SAKAYU SHIMIZU
  • Publication number: 20100285544
    Abstract: The invention relates to a novel aminotransferase, DNA encoding the enzyme, a recombinant vector into which the DNA has been introduced, and a transformant into which the vector has been introduced. Further, the invention also relates to a method for producing an optically active amino compound utilizing the enzyme or transformant. The aminotransferase of the invention has an ability of efficiently converting a ketone compound, particularly a cyclic ketone compound to an optically active amino compound. According to the invention, a method for efficiently producing an optically active amino compound, particularly an optically active cyclic amino compound is provided.
    Type: Application
    Filed: May 22, 2006
    Publication date: November 11, 2010
    Inventors: Noriyuki Ito, Yoshihiko Yasohara
  • Patent number: 7794993
    Abstract: The present invention relates to a polypeptide having an activity to asymmetrically reduce (3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanone to produce (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol isolated from a microorganism belonging to the genus Ogataea, a DNA encoding the polypeptide and a transformant that produces the polypeptide. The present invention moreover relates to a method of producing (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol utilizing the polypeptide or the transformant. Using the polypeptide or transformant of the present invention, optically active alcohols such as (2R,3S)-1-chloro-3-tert-butoxycarbonylamino-4-phenyl-2-butanol and the like can be produced efficiently.
    Type: Grant
    Filed: August 1, 2005
    Date of Patent: September 14, 2010
    Assignee: Kaneka Corporation
    Inventors: Noriyuki Kizaki, Miho Yano, Masahiro Funaki, Teruaki Takesue, Yoshihiko Yasohara, Souichi Morikawa, Takahisa Nakai, Michihiko Kataoka, Sakayu Shimizu
  • Publication number: 20100035317
    Abstract: An object of the present invention is to provide a novel alcohol dehydrogenase, a gene for the alcohol dehydrogenase, a vector including the gene, a transformant transformed with the vector, and a method for producing an optically active alcohol by utilizing them. A feature of the present invention directs to a novel polypeptide isolated from Candida maltosa, a DNA coding for the polypeptide, and a transformant producing the polypeptide. Another feature of the present invention directs to a method for producing an optically-active alcohol by reducing a carbonyl compound with the polypeptide or the transformant.
    Type: Application
    Filed: November 27, 2007
    Publication date: February 11, 2010
    Applicant: Kaneka Corporation
    Inventors: Shigeru Kawano, Takeru Ishige, Keita Iguchi, Tozo Nishiyama, Yoshihiko Yasohara
  • Publication number: 20090186391
    Abstract: The present invention has its object to provide a method of producing an erythro- or threo-2-amino-3-hydroxypropionic acid ester, and so forth. The present invention relates to: a method of asymmetrically reducing an N-2-amino-3-oxopropionic acid ester by allowing cells of a microorganism to act thereon; a polypeptide having an activity of asymmetrically reducing a carbonyl compound to give an optically active alcohol, which is isolated from a microorganism belonging to genus Brevundimonas; a DNA coding for the polypeptide; and a transformant producing the polypeptide. The invention also relates to a method of producing an optically active alcohol by reducing a carbonyl compound with the help of the polypeptide or the transformant.
    Type: Application
    Filed: March 29, 2007
    Publication date: July 23, 2009
    Applicant: Kaneka Corporation
    Inventors: Tozo Nishiyama, Yoshihiko Yasohara
  • Publication number: 20090148899
    Abstract: The present invention relates to a method for producing an optically-active amine compound. The method is characterized by using a transaminase (A), an ?-keto acid reductase (B), and an enzyme (C), each having specific properties, in an identical reaction system to convert a ketone compound into a corresponding optically-active amine compound in which a carbon atom with an amino group bonded thereto serves as an asymmetric point. The present invention also relates to a recombinant vector for use in the method. The present invention makes it possible to efficiently produce an optically-active amine compound.
    Type: Application
    Filed: May 28, 2007
    Publication date: June 11, 2009
    Inventors: Shigeru Kawano, Noriyuki Ito, Yoshihiko Yasohara
  • Patent number: 7531329
    Abstract: The present invention provides a novel polypeptide efficiently forming (R)-N-benzyl-3-pyrrolidinol, a polynucleotide coding for said polypeptide, and use of the same. The present invention relates to a polypeptide having the physical and chemical properties (1) to (4): (1) activity acting on N-benzyl-3-pyrrolidinone with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol; (2) optimum pH of 5.5 to 6.0 for activity ; (3) optimum temperature of 50° C. to 55° C. for activity ; (4) molecular weight of about 55,000 as determined by gel filtration analysis, and about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis. The present invention also relates to a polypeptide comprising the amino acid sequence of SEQ ID NO:1, a polynucleotide coding for said polypeptide, and a transformant producing said polypeptide at high levels.
    Type: Grant
    Filed: April 2, 2007
    Date of Patent: May 12, 2009
    Assignee: Kaneka Corporation
    Inventors: Noriyuki Kizaki, Tozo Nishiyama, Yoshihiko Yasohara