Abstract: The present invention provides a lipoxygenase that enables the highly efficient formation of an oxide of a highly unsaturated fatty acid or an oxide of a highly unsaturated fatty acid derivative from a highly unsaturated fatty acid or a highly unsaturated fatty acid derivative. The present invention provides a lipoxygenase that enables the highly efficient formation of 8-hydroxyeicosatetraenoic acid from arachidonic acid, the highly efficient formation of 8-hydroxyeicosapentaenoic acid from eicosapentaenoic acid, and the highly efficient formation of 10-hydroxy docosahexaenoic acid from docosahexaenoic acid. Specifically, the present invention provides a polynucleotide that comprises the nucleic acid sequence represented by SEQ ID NO: 1 and 3, or a homolog thereof, and a polypeptide that comprises the amino acid sequence represented by SEQ ID NO: 2 and 4, or a homolog thereof.

Abstract: Provided is a method for producing an unsaturated fatty acid oxide (e.g. ?3 fatty acid oxide) that is new and has more beneficial features compared to conventional technologies. This method uses a lipoxygenase-containing composition (e.g. a lipoxygenase-containing composition derived from beans) as a lipoxygenase enzyme, and also uses a freezing method with organic solvent extraction. The present invention makes it possible to produce an unsaturated fatty acid oxide (e.g. ?3 fatty acid oxide) efficiently and at a low cost. The present invention also provides a pharmaceutical composition containing an ?3 fatty acid oxide isolated and purified by this method as an active ingredient.

Abstract: The present invention provides a lipoxygenase that enables the highly efficient formation of an oxide of a highly unsaturated fatty acid or an oxide of a highly unsaturated fatty acid derivative from a highly unsaturated fatty acid or a highly unsaturated fatty acid derivative. The present invention provides a lipoxygenase that enables the highly efficient formation of 8-hydroxyeicosatetraenoic acid from arachidonic acid, the highly efficient formation of 8-hydroxyeicosapentaenoic acid from eicosapentaenoic acid, and the highly efficient formation of 10-hydroxy docosahexaenoic acid from docosahexaenoic acid. Specifically, the present invention provides a polynucleotide that comprises the nucleic acid sequence represented by SEQ ID NO: 1 and 3, or a homolog thereof, and a polypeptide that comprises the amino acid sequence represented by SEQ ID NO: 2 and 4, or a homolog thereof.

Abstract: Provided is a method for producing an unsaturated fatty acid oxide (e.g. ?3 fatty acid oxide) that is new and has more beneficial features compared to conventional technologies. This method uses a lipoxygenase-containing composition (e.g. a lipoxygenase-containing composition derived from beans) as a lipoxygenase enzyme, and also uses a freezing method with organic solvent extraction. The present invention makes it possible to produce an unsaturated fatty acid oxide (e.g. ?3 fatty acid oxide) efficiently and at a low cost. The present invention also provides a pharmaceutical composition containing an ?3 fatty acid oxide isolated and purified by this method as an active ingredient.

Abstract: A method of chemically extending a carbon chain of an unsaturated fatty acid for conversion into a different unsaturated fatty acid has been reported. The present invention shortens reaction steps of conventional methods, and completes a carbon chain extending reaction in a shorter time. The present invention provides a method of extending a carbon chain of an unsaturated fatty acid by two carbons, comprising steps of four stages including a short-path conversion reaction of an unsaturated fatty chain obtained from an unsaturated fatty acid into a malonic ester derivative, and heating of the malonic ester derivative to reflux in a lower fatty acid solution. The method of the present invention can complete a carbon chain extending reaction in a shorter time.

Abstract: A production method of highly unsaturated fatty acids with a high purity/high yield that compensates for shortcomings of conventional techniques is provided. A purification method of highly unsaturated fatty acids and/or derivatives thereof comprising (a) contacting and stirring first raw materials comprising the substances with a first silver salt aqueous solution to collect a first oil layer and a first aqueous layer; (b) separating the first aqueous layer into a second silver salt aqueous solution and the substances; and (c) contacting and stirring the first oil layer with the second silver salt aqueous solution for separation into an oil layer and an aqueous layer to obtain a second aqueous layer comprising the substances.

Abstract: The present invention provides a method of producing/purifying highly unsaturated fatty acids and/or derivatives thereof while suppressing deterioration of a silver salt aqueous solution, in production/purification of highly unsaturated fatty acids and/or derivatives thereof using the aqueous solution of silver salt. The present invention also contacts or mixes a mixture having a peroxide value (POV) of 10 or smaller with an aqueous solution of silver salt, in a method of purifying highly unsaturated fatty acids and/or derivatives thereof from a mixture comprising highly unsaturated fatty acids and/or derivatives thereof.

Abstract: A method of chemically extending a carbon chain of an unsaturated fatty acid for conversion into a different unsaturated fatty acid has been reported. The present invention shortens reaction steps of conventional methods, and completes a carbon chain extending reaction in a shorter time. The present invention provides a method of extending a carbon chain of an unsaturated fatty acid by two carbons, comprising steps of four stages including a short-path conversion reaction of an unsaturated fatty chain obtained from an unsaturated fatty acid into a malonic ester derivative, and heating of the malonic ester derivative to reflux in a lower fatty acid solution. The method of the present invention can complete a carbon chain extending reaction in a shorter time.

Abstract: The present invention provides a method of producing/purifying highly unsaturated fatty acids and/or derivatives thereof while suppressing deterioration of a silver salt aqueous solution, in production/purification of highly unsaturated fatty acids and/or derivatives thereof using the aqueous solution of silver salt. The present invention also contacts or mixes a mixture having a peroxide value (POV) of 10 or smaller with an aqueous solution of silver salt, in a method of purifying highly unsaturated fatty acids and/or derivatives thereof from a mixture comprising highly unsaturated fatty acids and/or derivatives thereof.

Abstract: A production method of highly unsaturated fatty acids with a high purity/high yield that compensates for shortcomings of conventional techniques is provided. A purification method of highly unsaturated fatty acids and/or derivatives thereof comprising (a) contacting and stirring first raw materials comprising the substances with a first silver salt aqueous solution to collect a first oil layer and a first aqueous layer; (b) separating the first aqueous layer into a second silver salt aqueous solution and the substances; and (c) contacting and stirring the first oil layer with the second silver salt aqueous solution for separation into an oil layer and an aqueous layer to obtain a second aqueous layer comprising the substances.

Abstract: A method of obtaining a polyunsaturated fatty acid derivative comprises contacting a mixture of fatty acid derivatives with a silver salt aqueous solution, and adjusting the free fatty acid content in the silver salt aqueous solution to 0.2 meq or less per gram of silver when repeatedly using the silver salt aqueous solution. A polyunsaturated fatty acid derivative having high purity and excellent quality can thus be economically obtained.

Abstract: A method of obtaining a polyunsaturated fatty acid derivative comprises contacting a mixture of fatty acid derivatives with a silver salt aqueous solution, and adjusting the free fatty acid content in the silver salt aqueous solution to 0.2 meq or less per gram of silver when repeatedly using the silver salt aqueous solution. A polyunsaturated fatty acid derivative having high purity and excellent quality can thus be economically obtained.

Abstract: This invention provides a low-temperature sintering conductive paste for high density circuit printing which can form a fine circuit having good adhesive force, a smooth surface and low resistance when applied on a substrate and then baked; the conductive paste of the invention uses, as conductive media, in combination with metal fillers having an average particle diameter of 0.

Abstract: To provide a method for producing a sensing film for gas sensors by sintering particles comprising a metal or a metal oxide on a substrate, where the sensing film for gas sensors having excellent sensor properties such as sensitivity and responsibility can be easily and simply formed. A nano-meter order particle (100), a dispersant (110) for preventing aggregation of the particles (100), and a scavenger (120) for trapping the dispersant (110) at the sintering are mixed in a solvent (130) to prepare a paste body (140), and this paste body (140) is coated on a base material and fired, thereby forming a sensing film.

Abstract: This invention provides a low-temperature sintering conductive paste for high density circuit printing which can form a fine circuit having good adhesive force, a smooth surface and low resistance when applied on a substrate and then baked; the conductive paste of the invention uses, as conductive media, in combination with metal fillers having an average particle diameter of 0.

Abstract: To provide a method for producing a sensing film for gas sensors by sintering particles comprising a metal or a metal oxide on a substrate, where the sensing film for gas sensors having excellent sensor properties such as sensitivity and responsibility can be easily and simply formed. A nano-meter order particle (100), a dispersant (110) for preventing aggregation of the particles (100), and a scavenger (120) for trapping the dispersant (110) at the sintering are mixed in a solvent (130) to prepare a paste body (140), and this paste body (140) is coated on a base material and fired, thereby forming a sensing film.

Abstract: [Object] A fatty acid-related polyunsaturated compound can be selectively purified using an industrially operable successive method under moderate conditions. [Content] A highly purified fatty acid-related polyunsaturated compound is obtained by a process comprising steps of forming a complex with silver on account of the high degree of unsaturation of the fatty acid-related polyunsaturated compound, isolating the complex from other fats on account of the hydrophilicity of the complex and then applying dissociating procedures to the complex to obtain the fatty acid-related polyunsaturated compound as a target substance on account of the hydrophobicity of the fatty acid-related polyunsaturated compound. As to the procedures for dissociation, addition of a dissociating agent and reduction of silver are disclosed. As to the dissociating agent, various compounds which produce insoluble composition with silver other then water are disclosed.