Patents by Inventor Yoshimi Kuroiwa

Yoshimi Kuroiwa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200236915
    Abstract: The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody ? light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the nonhuman animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.
    Type: Application
    Filed: September 12, 2019
    Publication date: July 30, 2020
    Inventors: Yoshimi KUROIWA, Kazuma TOMIZUKA, Hitoshi YOSHIDA, Isao ISHIDA
  • Patent number: 10448622
    Abstract: The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody ? light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the nonhuman animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.
    Type: Grant
    Filed: October 19, 2016
    Date of Patent: October 22, 2019
    Assignees: E. R. Squibb & Sons, L.L.C., Kyowa Hakko Kirin Co., Ltd
    Inventors: Yoshimi Kuroiwa, Kazuma Tomizuka, Hitoshi Yoshida, Isao Ishida
  • Patent number: 9902970
    Abstract: The invention relates to large-scale production of human antibodies by transgenic animals with high production of fully human IgG up to >10 g/L in sera with human IgG1 subclass dominancy. This invention also supports a feasibility of complex chromosome engineering for complicated genetic studies in non-murine mammalian species.
    Type: Grant
    Filed: August 5, 2013
    Date of Patent: February 27, 2018
    Assignee: SAB, LLC
    Inventors: Yoshimi Kuroiwa, Hiroaki Matsushita, Akiko Sano
  • Patent number: 9775332
    Abstract: A human artificial chromosome vector comprising a gene encoding a human antibody heavy chain, a gene encoding a human antibody light chain, and a gene encoding an IgM heavy chain constant region derived from a nonhuman animal.
    Type: Grant
    Filed: March 15, 2016
    Date of Patent: October 3, 2017
    Assignee: SAB, LLC
    Inventors: Yoshimi Kuroiwa, Hiroaki Matsushita, Akiko Kanai
  • Publication number: 20170099814
    Abstract: The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody ? light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the nonhuman animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.
    Type: Application
    Filed: October 19, 2016
    Publication date: April 13, 2017
    Applicants: E.R. Squibb & Sons, L.L.C., Kyowa Hakko Kirin Co. Ltd.
    Inventors: YOSHIMI KUROIWA, Kazuma Tomizuka, Hitoshi Yoshida, Isao Ishida
  • Patent number: 9499838
    Abstract: The present invention relates to a human artificial chromosome which is genetically transmissible to the next generation with high efficiency and the method for using the same. More specifically, the present invention relates to: a human artificial chromosome in which an about 3.5 Mb to about 1 Mb region containing an antibody ? light chain gene derived from human chromosome 22 is bound to a chromosome fragment which is transmissible to a progeny through a germ line of a non-human animal, said chromosome fragment is derived from another human chromosome; a non-human animal carrying the human artificial chromosome and an offspring thereof; a method for producing the non-human animal; a method for producing a human antibody using the nonhuman animal or an offspring thereof; and a human antibody-producing mouse carrying the human artificial chromosome.
    Type: Grant
    Filed: June 16, 2008
    Date of Patent: November 22, 2016
    Assignees: KYOWA HAKKO KIRIN CO., LTD., E. R. SQUIBB & SONS, L.L.C.
    Inventors: Yoshimi Kuroiwa, Kazuma Tomizuka, Hitoshi Yoshida, Isao Ishida
  • Publication number: 20160235045
    Abstract: A human artificial chromosome vector comprising a gene encoding a human antibody heavy chain, a gene encoding a human antibody light chain, and a gene encoding an IgM heavy chain constant region derived from a nonhuman animal.
    Type: Application
    Filed: March 15, 2016
    Publication date: August 18, 2016
    Inventors: Yoshimi KUROIWA, Hiroaki MATSUSHITA, Akiko KANAI
  • Patent number: 9315824
    Abstract: The present invention provides a human artificial chromosome vector comprising a gene encoding the human antibody heavy chain, a gene encoding the human antibody light chain, and a gene encoding IgM heavy chain constant region derived from a nonhuman animal; and being capable of producing a human antibody with a higher efficiency when the vector is introduced into an animal. By immunizing the animal produced using a human artificial chromosome vector of the present invention with a desired antigen, a large quantity of human polyclonal antibodies can be supplied.
    Type: Grant
    Filed: November 17, 2010
    Date of Patent: April 19, 2016
    Assignee: SAB, LLC
    Inventors: Yoshimi Kuroiwa, Hiroaki Matsushita, Akiko Sano
  • Publication number: 20150211020
    Abstract: The invention relates to large-scale production of human antibodies by transgenic animals with high production of fully human IgG up to >10 g/L in sera with human IgG1 subclass dominancy. This invention also supports a feasibility of complex chromosome engineering for complicated genetic studies in non-murine mammalian species.
    Type: Application
    Filed: August 5, 2013
    Publication date: July 30, 2015
    Inventors: Yoshimi Kuroiwa, Hiroaki Matsushita, Akiko Sano
  • Patent number: 8703482
    Abstract: The present invention relates to a human artificial chromosome (HAC) vector and a method for producing the same. The present invention further relates to a method for introducing foreign DNA using a human artificial chromosome vector and a method for producing a cell which expresses foreign DNA. Furthermore, the present invention relates to a method for producing a protein.
    Type: Grant
    Filed: October 7, 2010
    Date of Patent: April 22, 2014
    Assignee: Kyowa Hakko Kirin Co., Ltd
    Inventors: Mitsuo Oshimura, Motonobu Katoh, Kazuma Tomizuka, Yoshimi Kuroiwa, Minoru Kakeda
  • Publication number: 20120233715
    Abstract: A human artificial chromosome vector comprising a human antibody heavy chain gene, a human antibody light chain gene, and a human antibody surrogate light chain gene.
    Type: Application
    Filed: November 17, 2010
    Publication date: September 13, 2012
    Applicant: KYOWA HAKKO KIRIN CO., LTD
    Inventors: Yoshimi Kuroiwa, Hiroaki Matsushita, Akiko Sano
  • Publication number: 20120222140
    Abstract: The present invention provides a human artificial chromosome vector comprising a gene encoding the human antibody heavy chain, a gene encoding the human antibody light chain, and a gene encoding IgM heavy chain constant region derived from a nonhuman animal; and being capable of producing a human antibody with a higher efficiency when the vector is introduced into an animal. By immunizing the animal produced using a human artificial chromosome vector of the present invention with a desired antigen, a large quantity of human polyclonal antibodies can be supplied.
    Type: Application
    Filed: November 17, 2010
    Publication date: August 30, 2012
    Applicant: KYOWA HAKKO KIRIN CO., LTD.
    Inventors: Yoshimi Kuroiwa, Hiroaki Matsushita, Akiko Sano
  • Publication number: 20120093785
    Abstract: The present invention relates to a human artificial chromosome (HAC) vector and a method for producing the same. The present invention further relates to a method for introducing foreign DNA using a human artificial chromosome vector and a method for producing a cell which expresses foreign DNA. Furthermore, the present invention relates to a method for producing a protein.
    Type: Application
    Filed: October 7, 2010
    Publication date: April 19, 2012
    Inventors: Mitsuo Oshimura, Motonobu Katoh, Kazuma Tomizuka, Yoshimi Kuroiwa, Minoru Kakeda
  • Patent number: 8124406
    Abstract: The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.
    Type: Grant
    Filed: December 28, 2010
    Date of Patent: February 28, 2012
    Assignee: Kyowa Hakko Kirin Co., Ltd
    Inventors: Kazuma Tomizuka, Hitoshi Yoshida, Kazunori Hanaoka, Mitsuo Oshimura, Isao Ishida, Yoshimi Kuroiwa
  • Publication number: 20110231943
    Abstract: In general, the invention features genetically modified non-human mammals (e.g., bovines and other ungulates), and methods of making these mammals. In particular, the invention features transgenic ungulates having reduced levels of endogenous IgM heavy chain and/or prion protein.
    Type: Application
    Filed: April 6, 2011
    Publication date: September 22, 2011
    Applicant: KYOWA HAKKO KIRIN CO., LTD.
    Inventors: JAMES M. ROBL, YOSHIMI KUROIWA, POOTHAPPILLAI KASINATHAN, ISAO ISHIDA, KAZUMA TOMIZUKA
  • Publication number: 20110151518
    Abstract: The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.
    Type: Application
    Filed: December 28, 2010
    Publication date: June 23, 2011
    Inventors: Kazuma Tomizuka, Hitoshi Yoshida, Kazunori Hanaoka, Mitsuo Oshimura, Isao Ishida, Yoshimi Kuroiwa
  • Patent number: 7928285
    Abstract: In general, the invention features genetically modified non-human mammals (e.g., bovines and other ungulates), and methods of making these mammals. In particular, the invention features transgenic ungulates having reduced levels of endogenous IgM heavy chain and/or prion protein.
    Type: Grant
    Filed: June 25, 2008
    Date of Patent: April 19, 2011
    Assignee: Kyowa Hakko Kirin Co., Ltd.
    Inventors: James M. Robl, Yoshimi Kuroiwa, Poothappillai Kasinathan, Isao Ishida, Kazuma Tomizuka
  • Publication number: 20110067122
    Abstract: The present invention relates to the production of a transgenic ungulate which comprises a genetic modification that results in inactivation and loss of expression of its endogenous antibodies, and the expression of xenogenous antibodies, preferably human antibodies. This is effected by inactivation of the IgM heavy chain expression and, optionally, by inactivation of the Ig light chain expression, and by the further introduction of an artificial chromosome which results in the expression of non-bovine antibodies, preferably human antibodies.
    Type: Application
    Filed: February 9, 2009
    Publication date: March 17, 2011
    Applicant: Kyowa Hakko Kirin Co., Ltd.
    Inventors: James M. Robl, Richard A. Goldsby, Stacy E. Ferguson, Yoshimi Kuroiwa, Kazuma Tomizuka, Isao Ishida, Barbara A. Osborne
  • Patent number: 7868223
    Abstract: The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.
    Type: Grant
    Filed: May 7, 2008
    Date of Patent: January 11, 2011
    Assignee: Kyowa Hakko Kirin Co., Ltd.
    Inventors: Kazuma Tomizuka, Hitoshi Yoshida, Kazunori Hanaoka, Mitsuo Oshimura, Isao Ishida, Yoshimi Kuroiwa
  • Patent number: 7820878
    Abstract: The present invention relates to a method of producing an ungulate having both copies of the IgM heavy chain (mu) rag-1 and/or rag-2 gene eliminated from its genome. Animals which have IgM, rag-1 and/or rag-2 eliminated from their genome are unable to conduct the gene rearrangements that are necessary to generate the antigen receptors of B- or T-lymphocytes, and therefore will not develop native B- or T-cells. Because they are unable to produce B- and T-lymphocytes, these IgM, rag-1, or rag-2 ungulates cannot reject human hematopoietic stem cell preparations, and B- and T-lymphocytes which develop therefrom. Therefore, the present invention also involves injecting into IgM, rag-1, and/or rag-2 deficient ungulates, in utero or shortly after birth, human B- and T-lymphocytes whose immune systems produce human immunoglobulin that can be processed for therapeutic uses in humans.
    Type: Grant
    Filed: May 5, 2008
    Date of Patent: October 26, 2010
    Assignee: Kyowa Hakko Kirin Co., Ltd.
    Inventors: Richard A. Goldsby, James M. Robl, Barbara A. Osborne, Yoshimi Kuroiwa