Abstract: The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.

Abstract: The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.

Abstract: An automated analyzer includes a plurality of measurement units including a measurement section which measures a sample, a suction container connected to the plurality of measurement units through a first path, a vacuum exhaust system connected to the suction container through a second path, a discharge section connected to the suction container through a third path, a pressure adjustment mechanism disposed in the second path, and a control section which performs control such that the suction container is vacuum-exhausted by the vacuum exhaust system.

Abstract: An automated analyzer includes a plurality of measurement units including a measurement section which measures a sample, a suction container connected to the plurality of measurement units through a first path, a vacuum exhaust system connected to the suction container through a second path, a discharge section connected to the suction container through a third path, a pressure adjustment mechanism disposed in the second path, and a control section which performs control such that the suction container is vacuum-exhausted by the vacuum exhaust system.

Abstract: A light detection device comprises: a flow path section in which a plurality of lines of flow of a plurality of droplets 4 dispersed in oil and moving along a linear line of flow is retained on an array plane; a laser beam irradiation section which introduces a laser beam 3 in a direction in which the plurality of lines of flow are arrayed in the flow path section to irradiate the plurality of lines of flow; and a light detection section which detects emitted light generated from the plurality of lines of flow by the irradiation of the laser beam, from a vertical direction with respect to the array plane. A refractive index of the oil no and a refractive index of the droplets nd have a difference such that ?0.02?nd?no?0.05.

Abstract: The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.

Abstract: The present invention was made to provide novel methods for detecting DNA and devices therefor. Specifically, the present invention is a method of detecting DNA including the steps of: adding, to an oil, a fluorescently-labeled probe, a DNA intercalator, and a DNA solution containing a target DNA to produce droplets; performing PCR on the droplets; and measuring fluorescence from the fluorescently-labeled probe and fluorescence from the DNA intercalator, wherein the DNA solution has a concentration at which each droplet is produced so as to contain one or less target DNA molecule. In addition, the present invention is a DNA detection device including a droplet production unit, a thermal cycler unit, and a fluorescence detection unit which perform these steps.

Abstract: A light detection device comprises: a flow path section in which a plurality of lines of flow of a plurality of droplets 4 dispersed in oil and moving along a linear line of flow is retained on an array plane; a laser beam irradiation section which introduces a laser beam 3 in a direction in which the plurality of lines of flow are arrayed in the flow path section to irradiate the plurality of lines of flow; and a light detection section which detects emitted light generated from the plurality of lines of flow by the irradiation of the laser beam, from a vertical direction with respect to the array plane. A refractive index of the oil no and a refractive index of the droplets nd have a difference such that ?0.02?nd?no?0.05.

Abstract: To improve the detection sensitivity, detection accuracy, and reproducibility when electrostatic discharge is generated in a sample solution and analysis is performed using light emission in the generated plasma. A flow channel 100, which has cylindrical main portions each expanding conically from a narrow portion, is filled with a conductive sample solution, and an electric field is applied to the flow channel 100 to generate plasma in the generated air bubbles, so that the resulting light emission is measured.

Abstract: To improve detection sensitivity, detection accuracy, and reproducibility when electric discharge is caused in a sample solution to perform analysis with light emission in the plasma, a flow channel 101 provided with a narrow part is filled with a conductive sample solution, plasma is generated in bubbles formed by applying an electric field to the flow channel, and a region other than the narrow part in the flow channel is set as a measurement object region to measure light emission.

Abstract: To improve the detection sensitivity, detection accuracy, and reproducibility when electrostatic discharge is generated in a sample solution and analysis is performed using light emission in the generated plasma. A flow channel 100, which has cylindrical main portions each expanding conically from a narrow portion, is filled with a conductive sample solution, and an electric field is applied to the flow channel 100 to generate plasma in the generated air bubbles, so that the resulting light emission is measured.

Abstract: To improve detection sensitivity, detection accuracy, and reproducibility when electric discharge is caused in a sample solution to perform analysis with light emission in the plasma, a flow channel 101 provided with a narrow part is filled with a conductive sample solution, plasma is generated in bubbles formed by applying an electric field to the flow channel, and a region other than the narrow part in the flow channel is set as a measurement object region to measure light emission.

Abstract: The invention provides a method in which an annular or spiral droplet holder formed of wire is used to hold a droplet in a state of being hung therefrom or being contained therein. A means for moving the droplet holder is added to the droplet holder to enable droplet transfer. To merge two droplets, they are brought into contact. To drip the droplet held by a droplet holder formed of wire, the droplet holder is deformed using an external force. A light path which passes through a droplet is set to enable optical measurement. The present invention enables inexpensive, simple droplet transfer. An inexpensive, simple configuration for handling droplets in the fields of chemical analysis, biochemical analysis, and automatic blood analysis can be realized according to the present invention.

Abstract: A method of highly sensitive and quantitative luminescent analysis with the use of a detection device using a micro-channel and carrying a molecule which is capable of capturing a substance to be detected and bonded to a solid phase. A biochemical to be detected is captured in a channel-type device having a probe bonded to a solid phase. After labeling for luminescence, a luminescent reagent is flown thereto and the luminescence in the vicinity of the probe is optically detected.

Abstract: The present invention provides an apparatus for efficiently transporting or dispensing transport objects including not only particles but also liquid samples. A liquid in a first liquid transport pipe (3) is fed at a liquid feed velocity (V1), and a liquid droplet is formed toward an open end of a second liquid transport pipe (4) disposed with an air gap (11) in between. The particle is released into the liquid droplet, so that the particle is enclosed in the liquid droplet. Suction with a liquid feed velocity (V2) is applied to the inside of the second liquid transport pipe. Since the relationship between V1 and V2 is V1<V2, the liquid droplet, after reaching the open end (10) of the second liquid transport pipe, is sheared off by suction, thereby forming a liquid section in the second liquid transport pipe.

Abstract: A device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution. A chemical analysis device is also provided to capture molecules in the solution and subsequently measure the captured molecules. Reaction efficiency and sample throughput are thereby improved. The chemical reaction and chemical analysis devices use a microfluidic device channel as a reaction vessel. The channel is provided with a particular molecule immobilized on an interior surface of the channel with an obstacle positioned against the flow. In a typical reaction vessel having an enzyme immobilized in the capillary's interior surface and glass beads as obstacles, a reaction solution can move either in one direction or back and forth to react with the immobilized enzyme. The flow of the reaction solution is not laminar such that a reaction between the particular molecule and the reaction solution proceeds at high efficiency.

Abstract: A bead array chip manufacturing process for manufacturing a bead array chip having plural kinds of beads arrayed in a predetermined order in, in a container having a plurality of first channels disposed substantially in parallel with each other and a second channel crossing the plurality of first channels, each of the first channels. The process including lowering a capillary inserted in the second channel and sucking and retaining one bead in one end of the capillary, lifting the capillary to position the beads retained in the one end of the capillary in a desired channel of the plurality of first channels, terminating the suction of the capillary, and generating a uniflow of a fluid in the second channel.

Abstract: A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel.

Abstract: A chemical reaction device is provided for a chemical reaction between molecules immobilized on a solid phase and molecules in a solution, and a chemical analysis device is also provided to capture molecules in the solution by molecules immobilized on the solid phase through a chemical reaction and subsequent measurement of the captured molecules. Reaction efficiency as well as sample throughput are thereby improved. The chemical reaction device and the chemical analysis device use a channel of a microfluidic device for a reaction vessel, and at least a particular molecule is immobilized on an interior surface and a fixed structure or a non-fixed obstacle against a flow is provided in the channel.

Abstract: A method of highly sensitive and quantitative luminescent analysis with the use of a detection device using a micro-channel and carrying a molecule which is capable of capturing a substance to be detected and bonded to a solid phase. A biochemical to be detected is captured in a channel-type device having a probe bonded to a solid phase. After labeling for luminescence, a luminescent reagent is flown thereto and the luminescence in the vicinity of the probe is optically detected.