Patents by Inventor Yoshinori Fujiyoshi
Yoshinori Fujiyoshi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11261426Abstract: Objects of the present invention are to provide a method for directly obtaining pluripotent stem cells which do not have tumorigenic property from body tissue and the thus obtained pluripotent stem cells. The present invention relates to SSEA-3 (+) pluripotent stem cells that can be isolated from body tissue.Type: GrantFiled: June 22, 2016Date of Patent: March 1, 2022Inventors: Mari Dezawa, Yoshinori Fujiyoshi, Youichi Nabeshima
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Patent number: 10541107Abstract: A three-dimensional image reconstruction method associated with the present invention includes the steps of: obtaining a first transmission electron microscope image of a sample containing the membrane proteins present within a lipid membrane, the image having been taken by illuminating an electron beam on the sample from a direction tilted relative to a line normal to the membrane surface of the lipid membrane; obtaining a second transmission electron microscope image of the sample taken by illuminating the electron beam on the sample perpendicularly to the membrane surface of the lipid membrane; identifying orientations of the membrane proteins of the first transmission electron microscope image on a basis of the second transmission electron microscope image; and analyzing a three-dimensional structure of the membrane proteins from the first transmission electron microscope image on a basis of information about the identified orientations of the membrane proteins.Type: GrantFiled: August 21, 2015Date of Patent: January 21, 2020Assignees: National University Corporation Nagoya University, JEOL Ltd.Inventors: Yoshinori Fujiyoshi, Isamu Ishikawa, Naoki Hosogi
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Patent number: 10369162Abstract: The purpose of the present invention is to identify a migratory factor that guides pluripotent stem cells (Muse cells) useful in new medical applications to damage, and to provide a pharmaceutical composition that includes the migratory factor for promoting tissue regeneration in regenerative medicine that makes use of Muse cells. In the present invention, a receptor that is specifically expressed in Muse cells rather than non-Muse cells was identified, and it was confirmed that a ligand for this receptor can function as a migratory factor. In the present invention, sphingosine-1-phosphate (S1P) was identified as a migratory factor, and thus, the present invention pertains to a pharmaceutical composition for guiding pluripotent stem cells to damage, the composition including S1P as an active ingredient.Type: GrantFiled: July 6, 2017Date of Patent: August 6, 2019Assignees: CLIO, INC., TOHOKU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITYInventors: Mari Dezawa, Yoshinori Fujiyoshi, Masanori Yoshida
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Publication number: 20180355313Abstract: Objects of the present invention are to provide a method for directly obtaining pluripotent stern cells which do not have tumorigenic property from body tissue and the thus obtained pluripotent stem cells. The present invention relates to SSEA-3 (+) pluripotent stern cells that can be isolated from body tissue.Type: ApplicationFiled: August 17, 2018Publication date: December 13, 2018Inventors: Mari Dezawa, Yoshinori Fujiyoshi, Youichi Nabeshima, Shohei Wakao, Masanori Yoshida, Yasumasa Kuroda
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Patent number: 10034889Abstract: The purpose of the present invention is to identify a migratory factor that guides pluripotent stem cells (Muse cells) useful in new medical applications to damage, and to provide a pharmaceutical composition that includes the migratory factor for promoting tissue regeneration in regenerative medicine that makes use of Muse cells. In the present invention, a receptor that is specifically expressed in Muse cells rather than non-Muse cells was identified, and it was confirmed that a ligand for this receptor can function as a migratory factor. In the present invention, sphingosine-1-phosphate (S1P) was identified as a migratory factor, and thus, the present invention pertains to a pharmaceutical composition for guiding pluripotent stem cells to damage, the composition including S1P as an active ingredient.Type: GrantFiled: August 16, 2016Date of Patent: July 31, 2018Assignees: CLIO, INC., TOHOKU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITYInventors: Mari Dezawa, Yoshinori Fujiyoshi, Masanori Yoshida
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Publication number: 20170304326Abstract: The purpose of the present invention is to identify a migratory factor that guides pluripotent stem cells (Muse cells) useful in new medical applications to damage, and to provide a pharmaceutical composition that includes the migratory factor for promoting tissue regeneration in regenerative medicine that makes use of Muse cells. In the present invention, a receptor that is specifically expressed in Muse cells rather than non-Muse cells was identified, and it was confirmed that a ligand for this receptor can function as a migratory factor. In the present invention, sphingosine-1-phosphate (S1P) was identified as a migratory factor, and thus, the present invention pertains to a pharmaceutical composition for guiding pluripotent stem cells to damage, the composition including S1P as an active ingredient.Type: ApplicationFiled: July 6, 2017Publication date: October 26, 2017Applicants: CLIO, INC., TOHOKU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITYInventors: Mari Dezawa, YOSHINORI FUJIYOSHI, MASANORI YOSHIDA
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Publication number: 20170278669Abstract: A three-dimensional image reconstruction method associated with the present invention comprises the steps of: obtaining a first transmission electron microscope image of a sample containing the membrane proteins present within a lipid membrane, the image having been taken by illuminating an electron beam on the sample from a direction tilted relative to a line normal to the membrane surface of the lipid membrane (step S10); obtaining a second transmission electron microscope image of the sample taken by illuminating the electron beam on the sample perpendicularly to the membrane surface of the lipid membrane (step S12); identifying orientations of the membrane proteins of the first transmission electron microscope image on a basis of the second transmission electron microscope image (step S14); and analyzing a three-dimensional structure of the membrane proteins from the first transmission electron microscope image on a basis of information about the identified orientations of the membrane proteins (step S18)Type: ApplicationFiled: August 21, 2015Publication date: September 28, 2017Inventors: Yoshinori Fujiyoshi, Isamu Ishikawa, Naoki Hosogi
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Patent number: 9550975Abstract: Objects of the present invention are to provide a method for directly obtaining pluripotent stem cells from body tissue and the thus obtained pluripotent stem cells. The present invention relates to SSEA-3 (+) pluripotent stem cells that can be isolated from body tissue.Type: GrantFiled: July 14, 2010Date of Patent: January 24, 2017Inventors: Mari Dezawa, Yoshinori Fujiyoshi, Youichi Nabeshima, Shohei Wakao
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Publication number: 20160369232Abstract: Objects of the present invention are to provide a method for directly obtaining pluripotent stem cells which do not have tumorigenic property from body tissue and the thus obtained pluripotent stem cells. The present invention relates to SSEA-3 (+) pluripotent stem cells that can be isolated from body tissue.Type: ApplicationFiled: June 22, 2016Publication date: December 22, 2016Inventors: Mari Dezawa, Yoshinori Fujiyoshi, Youichi Nabeshima, Shohei Wakao, Masanori Yoshida, Yasumasa Kuroda
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Publication number: 20160354393Abstract: The purpose of the present invention is to identify a migratory factor that guides pluripotent stem cells (Muse cells) useful in new medical applications to damage, and to provide a pharmaceutical composition that includes the migratory factor for promoting tissue regeneration in regenerative medicine that makes use of Muse cells. In the present invention, a receptor that is specifically expressed in Muse cells rather than non-Muse cells was identified, and it was confirmed that a ligand for this receptor can function as a migratory factor. In the present invention, sphingosine-1-phosphate (S1P) was identified as a migratory factor, and thus, the present invention pertains to a pharmaceutical composition for guiding pluripotent stem cells to damage, the composition including S1P as an active ingredient.Type: ApplicationFiled: August 16, 2016Publication date: December 8, 2016Inventors: MARI DEZAWA, YOSHINORI FUJIYOSHI, MASANORI YOSHIDA
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Patent number: 9446033Abstract: The purpose of the present invention is to identify a migratory factor that guides pluripotent stem cells (Muse cells) useful in new medical applications to damage, and to provide a pharmaceutical composition that includes the migratory factor for promoting tissue regeneration in regenerative medicine that makes use of Muse cells. In the present invention, a receptor that is specifically expressed in Muse cells rather than non-Muse cells was identified, and it was confirmed that a ligand for this receptor can function as a migratory factor. In the present invention, sphingosine-1-phosphate (S1P) was identified as a migratory factor, and thus, the present invention pertains to a pharmaceutical composition for guiding pluripotent stem cells to damage, the composition including S1P as an active ingredient.Type: GrantFiled: February 28, 2014Date of Patent: September 20, 2016Assignees: CLIO, INC., TOHOKU UNIVERSITY, NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITYInventors: Mari Dezawa, Yoshinori Fujiyoshi, Masanori Yoshida
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Patent number: 9399758Abstract: Objects of the present invention are to provide a method for directly obtaining pluripotent stem cells which do not have tumorigenic property from body tissue and the thus obtained pluripotent stem cells. The present invention relates to SSEA-3 (+) pluripotent stem cells that can be isolated from body tissue.Type: GrantFiled: March 30, 2012Date of Patent: July 26, 2016Inventors: Mari Dezawa, Yoshinori Fujiyoshi, Youichi Nabeshima
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Publication number: 20160008340Abstract: The purpose of the present invention is to identify a migratory factor that guides pluripotent stem cells (Muse cells) useful in new medical applications to damage, and to provide a pharmaceutical composition that includes the migratory factor for promoting tissue regeneration in regenerative medicine that makes use of Muse cells. In the present invention, a receptor that is specifically expressed in Muse cells rather than non-Muse cells was identified, and it was confirmed that a ligand for this receptor can function as a migratory factor. In the present invention, sphingosine-1-phosphate (S1P) was identified as a migratory factor, and thus, the present invention pertains to a pharmaceutical composition for guiding pluripotent stem cells to damage, the composition including S1P as an active ingredient.Type: ApplicationFiled: February 28, 2014Publication date: January 14, 2016Inventors: Mari Dezawa, Yoshinori Fujiyoshi, Masanori Yoshida
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Publication number: 20120244129Abstract: Objects of the present invention are to provide a method for directly obtaining pluripotent stem cells which do not have tumorigenic property from body tissue and the thus obtained pluripotent stem cells. The present invention relates to SSEA-3 (+) pluripotent stem cells that can be isolated from body tissue.Type: ApplicationFiled: March 30, 2012Publication date: September 27, 2012Inventors: Mari Dezawa, Yoshinori Fujiyoshi, Youichi Nabeshima, Shohei Wakao, Masanori Yoshida, Yasumasa Kuroda
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Patent number: 8008633Abstract: A charged-particle beam system is offered which is equipped with a Z-motion mechanism to enable tomography. The Z-motion mechanism includes a rotary disk having three tapering surfaces on which balls are nested. The rotary disk is rotated via a worm gear to cause the balls to go upward along the tapering surfaces. This pushes an overlying elevatable disk upward, i.e., in the Z-direction. Consequently, the specimen stage is pushed up in the Z-direction.Type: GrantFiled: March 28, 2008Date of Patent: August 30, 2011Assignee: Jeol, Ltd.Inventors: Yoshinori Fujiyoshi, Tomohisa Fukuda
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Publication number: 20110070647Abstract: Objects of the present invention are to provide a method for directly obtaining pluripotent stem cells from body tissue and the thus obtained pluripotent stem cells. The present invention relates to SSEA-3 (+) pluripotent stem cells that can be isolated from body tissue.Type: ApplicationFiled: July 14, 2010Publication date: March 24, 2011Inventors: Mari DEZAWA, Yoshinori FUJIYOSHI, Youichi NABESHIMA, Shohei WAKAO
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Publication number: 20090230319Abstract: A charged-particle beam system is offered which is equipped with a Z-motion mechanism to enable tomography. The Z-motion mechanism includes a rotary disk having three tapering surfaces on which balls are nested. The rotary disk is rotated via a worm gear to cause the balls to go upward along the tapering surfaces. This pushes an overlying elevatable disk upward, i.e., in the Z-direction. Consequently, the specimen stage is pushed up in the Z-direction.Type: ApplicationFiled: March 28, 2008Publication date: September 17, 2009Applicant: JEOL, LTDInventors: Yoshinori Fujiyoshi, Tomohisa Fukuda
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Patent number: 6304105Abstract: The level shifter circuit includes a low-voltage operating inverter INV4, a high-voltage operating inverter INV5, NMOS transistors NT5 and NT6 and a PMOS transistor PT3. The output from low-voltage operating inverter INV4, the source of NMOS transistor NT6 and the gate of PMOS transistor PT3 are joined. The input to high-voltage operating inverter INV5, the drain of NMOS transistor NT6 and the source of NMOS transistor PT5 and that of PMOS transistor PT3 are joined. The drain of NMOS transistor NT5 and the gates of NMOS transistors NT5 and NT6 are connected to the power feed line of a high-voltage power source. The input to low-voltage operating inverter INV4 forms an input signal terminal Vin2 while the output from high-voltage operating inverter INV5 forms an output signal terminal Vout2.Type: GrantFiled: May 2, 2000Date of Patent: October 16, 2001Assignee: Sharp Kabushiki KaishaInventor: Yoshinori Fujiyoshi
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Patent number: 5406087Abstract: There is disclosed a simple specimen-holding device for use with an electron microscope. The device comprises a pair of films which transmit the electron beam. The films are reinforced with a network of reinforcing members and placed on a specimen stage. A specimen to be investigated is held in a thin space formed between the films, together with moisture. The fringes of the films are compressed together to hermetically isolate the space between the films from the specimen chamber of the microscope which is evacuated. The electron beam passes through the thin space sandwiched between the films. This permits the specimen to be observed in an almost unmodified state with little damage to the specimen.Type: GrantFiled: January 18, 1994Date of Patent: April 11, 1995Assignees: Protein Engineering Research Institute, JEOL Ltd.Inventors: Yoshinori Fujiyoshi, Mikio Naruse