Abstract: Objects of the present invention are to provide a base film for cell culture that enables mass production of homogeneous and high-quality cell aggregates even without the use of a biological serum, and to provide a base film-forming agent for forming the base film, and a substrate for producing cell aggregate. The present inventors provide a base film-forming agent for cell culture including a polymer, a cell adhesive substance, and a solvent, wherein the polymer contains a repeating unit derived from a monomer of formula (I) below: wherein Ua1, Ua2, Ra1, and Ra2 are as described in the specification and the claims.

Abstract: The invention provides a structural base that includes a coating film on at least a portion of the surface thereof, the coating film having a difference between the maximum film thickness and the minimum film thickness of not more than 1000 ?. A method of the invention produces such structural bases. The coating film includes a copolymer including a repeating unit containing a group represented by formula (a), and a repeating unit containing a group represented by formula (b): wherein Ua1, Ua2, Ub1, Ub2, Ub3 and An? are as defined herein.

Abstract: Provided is a coating film which is obtained by applying a composition for forming a coating film, the composition containing a copolymer, and which is used to suppress adhesion of a biological substance, in which the copolymer is water-insoluble, the copolymer contains a repeating unit (A) represented by the following Formula (A) and a repeating unit (B) represented by the following Formula (B), and a molar ratio (A:B) of the repeating unit (A) to the repeating unit (B) in the copolymer is 89:11 to 50:50. (In the formula, R1 to R3 each independently represent a hydrogen atom or an alkyl group having 1 to 5 carbon atoms, and X1 and X2 each independently represent a single bond, an ester bond, an ether bond, an amide bond, or an alkylene group having 1 to 5 carbon atoms which may be interrupted by an oxygen atom.

Abstract: The invention provides a method for producing a polymer used as a base film for cell culture which comprises (i) preparing a mixture containing (a) a monomer of formula (I): wherein Ua1 and Ua2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Ra1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Ra2 represents a linear or branched alkylene group having 1 to 5 carbon atoms, (b) a radical polymerization initiator, and (c) an organic solvent, and (ii) preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer. The invention also provides methods of producing a base film for cell culture and a cell culture container.

Abstract: Methods for producing the amorphous metal oxide semiconductor layer where amorphous metal oxide semiconductor layer is formed by use of a precursor composition containing a metal salt, a primary amide, and a water-based solution. The methodology for producing the amorphous metal oxide semiconductor layer includes applying the precursor composition onto a substrate to form a precursor film, and firing the film at a temperature of 150° C. or higher and lower than 300° C.

Abstract: The invention provides a method for producing a polymer used as a base film for cell culture which comprises (i) preparing a mixture containing (a) a monomer of formula (I): wherein Ua1 and Ua2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Ra1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Ra2 represents a linear or branched alkylene group having 1 to 5 carbon atoms, (b) a radical polymerization initiator, and (c) an organic solvent, and (ii) preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer. The invention also provides methods of producing a base film for cell culture and a cell culture container.

Abstract: The invention provides a composition for forming a coating film having an ability to suppress adhesion of biological substances, a coating film using the same, a substrate for cell culture using the same, a laminated film and a method for producing a cell aggregate(s). The composition for forming a coating film comprises a polymer containing unit structures represented by the formula (1) and the formula (2): wherein R1 to R3, X1, X2, T1, n1, n2 and n3 are as defined herein but not containing a polysiloxane skeleton, and a solvent.

Abstract: The invention provides a composition for forming a coating film having compatibility with a biological substance which comprises a block copolymer having unit structures represented by the formula (1) and the formula (2): (wherein R1 to R3, U1 and U2 each independently represent a hydrogen atom or an alkyl group having 1 to 5 carbon atoms, X1 and X2 represent an alkylene group having 1 to 5 carbon atoms, and n1 represents an integer of 1 to 10), and a solvent. The invention also provides a coating film using the same, a substrate for cell culture using the same, a method for producing the substrate for cell culture, and a method for producing a cell aggregate.

Abstract: The invention provides a composition for forming a coating film containing a phosphoric acid group-containing polymer capable of forming a film on a substrate by a simple process and capable of maintaining film properties under a solvent environment containing water, and a coating film which is a cured product of the composition. In particular, the composition for forming a coating film contains (a) a polymer (P) containing a hydroxyl group, and the hydroxyl group is derived only from a phosphoric acid group, (b) a polycarbodiimide (C) containing a structure represented by the following formula (c-1): —N?C?N—??(c-1), and (c) a solvent.

Abstract: The invention provides a resin molding and a method of producing the same in which sterilizability thereof is secured and discoloration (yellowing) thereof is reduced even when the resin molding is radiation-irradiated under anaerobic conditions (deoxidation conditions). The radiation-irradiated and packaged resin molding is primarily packaged with a packaging material having oxygen permeability and then secondarily packaged with an oxygen impermeable packaging material together with a deoxidizer. The method of producing a radiation-irradiated and packaged resin molding involves a step of primarily packaging a resin molding before radiation irradiation with a packaging material having oxygen permeability, a step of subsequently secondarily packaging with an oxygen impermeable packaging material together with a deoxidizer, and then a step of conducting radiation irradiation. A step of opening the secondary packaging under the atmosphere, following the radiation irradiation step, is preferred.

Abstract: A substrate for producing cell aggregates provided with a plurality of spots which comprises a polymer containing a recurring unit derived from a monomer represented by the following formula (I): wherein Ua1, Ua2, Ra1 and Ra2 are as defined herein, on a substrate having an ability to inhibit adhesion of cells, wherein a ratio of a total area of the spots to a surface area of the substrate is 30% or more, a diameter of each spot is 50 to 5,000 ?m, and a distance between the spots is 30 to 1,000 ?m, a method for producing the same, and a method for producing cell aggregates using such a substrate and a method of improving cell utilization efficiency at the time of producing the cell aggregates.

Abstract: Provided are a composition for forming a coating film, a coating film and a substrate having the coating film, which have excellent compatibility with biological substances, as well as a method for producing the same. A composition for forming a coating film having the ability to inhibit adhesion of biological substances, the composition comprising a copolymer and a solvent, wherein the copolymer is obtained by subjecting a monomer mixture to polymerization, wherein the monomer mixture comprises compounds represented by the following formulae (1), (2), and (3): wherein R1 to R6, n, and m are as defined in the Description and Claims, wherein the compound represented by the formula (3) above is contained in a predetermined amount in the monomer mixture, a coating film obtained from the composition, and a substrate having the coating film as well as a method for producing the same.

Abstract: An object of the present invention is to provide a cryopreservation vessel which has a coating that is capable of inhibiting the adhesion of cells or proteins and is resistant not only to aqueous solvents but also to organic solvents (for example, cryoprotectants, in particular, dimethyl sulfoxide). The invention provides a cell or protein cryopreservation vessel including, on at least a portion of the surface thereof, a coating that includes a repeating unit containing a group represented by the following formula (a), and a repeating unit containing a group represented by the following formula (b) (wherein Ua1, Ua2, Ua3, Ub1, Ub2, Ub3 and An? are as defined in the description and the claims).

Abstract: Methods for producing the amorphous metal oxide semiconductor layer where amorphous metal oxide semiconductor layer is formed by use of a precursor composition containing a metal salt, a primary amide, and a water-based solution. The methodology for producing the amorphous metal oxide semiconductor layer includes applying the precursor composition onto a substrate to form a precursor film, and firing the film at a temperature of 150° C. or higher and lower than 300° C.

Abstract: This is to provide a coating film having a function of inhibiting adhesion of a biological substance, a method for manufacturing the coating film, a copolymer obtainable by polymerizing a specific monomer mixture, a composition for forming a coating film having a specific composition, a method for manufacturing a varnish containing a copolymer to be used as a raw material of the composition for forming a coating film which is used for forming said film, and a sol for forming the coating film. In particular, this is to provide a coating film obtained by the method comprising a process of applying a composition for forming a coating film which comprises a copolymer comprising a recurring unit containing an organic group of the formula (a) and a recurring unit containing an organic group of the formula (b) and a solvent onto a substrate; and a process of drying at a temperature of ?200° C. to 200° C. (wherein Ua1, Ua2, Ub1, Ub2 and Ub3, and An? are as defined in the present specification and the claims).

Abstract: The invention provides a method for producing a copolymer-containing varnish using a monomer having a specific unsaturated bond which does not cause the problem of gelation, a method for producing a composition, a method for producing a coating film, preferably compatible with biomaterials. The method for producing a copolymer-containing varnish comprises copolymerizing a monomer mixture containing compounds of formulas (1) and (2): wherein R1 represents a hydrogen atom or a methyl group, R2 represents an alkylene group having 1 to 6 carbon atoms, n represents an integer of 1 to 30, R11 represents a hydrogen atom or a methyl group, and A1 represents a monovalent organic group having a cationic property, in a solvent for polymerization, in the presence of a radical polymerization initiator, wherein % by mass of the compound of formula (1) in a phosphorus-containing compound contained in the monomer mixture is 70% by mass or more.

Abstract: The invention provides a method for producing a polymer used as a base film for cell culture which comprises (i) preparing a mixture containing (a) a monomer of formula (I): wherein Ua1 and Ua2 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, Ra1 represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms, and Ra2 represents a linear or branched alkylene group having 1 to 5 carbon atoms, (b) a radical polymerization initiator, and (c) an organic solvent, and (ii) preparing a polymer by raising a temperature of the mixture under stirring to polymerize the monomer. The invention also provides methods of producing a base film for cell culture and a cell culture container.

Abstract: The invention provides a cell culture vessel with a coating which inhibits adhesion of a peptide relating to cell proliferation on at least a part of the surface thereof. The coating desirably contains a copolymer containing a recurring unit which contains a group represented by formula (a) and a recurring unit which contains a group represented by formula (b). wherein Ua1, Ua2, Ua3, Ub1, Ub2, Ub3 and An? are as defined herein.

Abstract: The invention provides a coating agent for a flow passage which comprises a copolymer containing a recurring unit which contains an organic group of the formula (a) wherein Ua1 and Ua2 are defined herein, and a recurring unit which contains an organic group of the formula (b) wherein Ub1, Ub2, Ub3, and An? are defined herein. The invention also provides a flow passage device having the coating agent for a flow passage on at least part of an inner surface of a flow passage, a platelet-producing flow passage device and a method for manufacturing the same.

Abstract: The present invention provides a cell culture container for long term non-adherent cell culture which comprises having a coating containing a copolymer which is a copolymer having a recurring unit which contains a group represented by the following formula (a), a recurring unit which contains a group represented by the following formula (b), and a recurring unit which contains a group represented by the following formula (c), and a molar ratio of the recurring unit which contains a group represented by the formula (c) being 1 to 50 mol % based on the whole copolymer, provided with at least a part of a surface thereof [wherein, Ua1, Ua2, Ub1, Ub2 and Ub3, Rc, and An? are as defined in the specification and Claims.