Abstract: A nucleic acid sequence measurement method for measuring a target RNA having a specific nucleic acid sequence included in a cell suspension by hybridization, including a step of heating the cell suspension at 100° C. to 200° C. in a pressurized state to obtain an RNA extract, a step of adding a proteolytic enzyme to the RNA extract to cause the RNA extract to be reacted, thereby preparing a sample solution, a step of supplying the sample solution to a device for nucleic acid sequence measurement, the device being equipped with a fluorescent probe that hybridizes with the target RNA, a step of subjecting the target RNA and the fluorescent probe to a hybridization reaction, and a step of measuring fluorescence from the device for nucleic acid sequence measurement.

Abstract: A method for producing a complex, a method for determining microbial inclusion, and a method for identifying the included microorganism, which can be easily performed is provided. A method for producing a complex containing a target that is contained in a sample, includes a step (a) of bringing a sample into contact with a label molecule A, a label molecule B, and a label molecule C and forming a complex, and a step (b) of isolating the complex. The target is a nucleic acid. The label molecule A contains a nucleic acid sequence capable of hybridizing to a part of the target and substantially complementary to the nucleic acid sequence of the target. The label molecule B contains a nucleic acid sequence capable of hybridizing to a part of the target and substantially complementary to the nucleic acid sequence of the target.

Abstract: A water treatment method including: a concentration step of concentrating microorganisms in sample water to purify a concentrate; a detection step of detecting an inhibitor contained in the sample water or the concentrate; a determination step of determining whether to perform a removal step based on a result of the detection in the detection step; the removal step of removing the inhibitor from the concentrate in a case where the removal step is determined to be performed in the determination step; and a measurement step performed after the determination step or the removal step, of measuring the microorganisms contained in the concentrate.

Abstract: A target (50) having a specific nucleic acid sequence included in a sample is measured using a detection probe (10) that has a detection part having a nucleic acid sequence complementary to the nucleic acid sequence of the target (50), a label molecule A (20) having a nucleic acid sequence that is complementary to the nucleic acid sequence of the target (50) and different from the nucleic acid sequence of the detection part, and a label molecule B (30) having a nucleic acid sequence complementary to the nucleic acid sequence of the label molecule A (20), in which a fluorescent molecule is attached to either one of the label molecule A (20) and the label molecule B (30), and the label molecule A (20) and the label molecule B (30) bind to each other at at least two or more sites to form a branched structural body of the label molecule A (20) and the label molecule B (30).

Abstract: A target measurement method for measuring a target contained in a sample, includes, when a solution containing a light-absorbing substance that absorbs excitation light that excites a fluorescent molecule or fluorescence emitted from the fluorescent molecule is in contact with a solid-phase surface of a substrate that is provided with a bound product of the target and a capture molecule specifically binding to the target which is modified with a fluorescent molecule, measuring a fluorescence obtained by irradiation of the excitation light from an opposite side to the solid-phase surface of the substrate from the opposite side to the solid-phase surface of the substrate.

Abstract: A technique involves outputting not only the condition of hydraulic oil but also information on failure prediction of equipment by processing in real time. Such a technique utilizes an oil condition sensor attached to the equipment, a parameter calculator that calculates the values of a plurality of parameters indicating the state of the hydraulic oil based on the sensor output of the oil condition sensor and correlation information between the sensor output and the values of a plurality of parameters indicating the state of the hydraulic oil, and failure prediction determiner that predicts equipment failures based on parameter values and identifies and outputs parameters that are inferred as the cause of the failure prediction, and outputs not only the condition of hydraulic oil but also information on failure prediction of the equipment by processing in real time.

Abstract: A nucleic acid sequence measuring apparatus (1) measures a target (TG) having a specific nucleic acid sequence included in a sample.

Abstract: When the target (30) is not supplied, the binding via the binding part is maintained, and when the donor fluorescent molecule (11) is excited, energy is transferred to the acceptor fluorescent molecule (21) that is close to the donor fluorescent molecule (11). Then, the acceptor fluorescent molecule (21) exhibits fluorescence. When the target (30) is supplied, the target (30) is bound to the detection part to release the binding via the binding part, and the acceptor fluorescent molecule (21) is separated from the donor fluorescent molecule (11). Then, the donor fluorescent molecule (11) exhibits fluorescence.

Abstract: To reduce optical noise in fluorescence measurement. A biochip 110 for fluorescence measurement includes a transparent substrate 111, multiple microlenses 112 dispersively formed on a first surface 111a of the transparent substrate 111, multiple protruding portions 113 formed corresponding one-to-one with the microlenses 112 on a second surface 111b of the transparent substrate 111, and a fluorescence measurement site 114 formed at a top portion of each protruding portion 113.

Abstract: In a case where the target (30) is not supplied, binding through a nucleic acid sequence from the base end on a side of the solid phase surface of the complementary sequence complementary to a part of the nucleic acid sequence of the detection sequence to the other base end is maintained, which causes fluorescence of the fluorescent molecule (11) to be quenched by the quenching molecule (21) close to the fluorescent molecule (11). In a case where the target (30) is supplied, the target is bound to the detection sequence and the binding through the complementary sequence is released, which causes the fluorescent molecule (11) to separate from the quenching molecule (21) and emit fluorescence.

Abstract: To reduce optical noise in fluorescence measurement. A biochip 110 for fluorescence measurement includes a transparent substrate 111, multiple microlenses 112 dispersively formed on a first surface 111a of the transparent substrate 111, multiple protruding portions 113 formed corresponding one-to-one with the microlenses 112 on a second surface 111b of the transparent substrate 111, and a fluorescence measurement site 114 formed at a top portion of each protruding portion 113.

Abstract: In this biological information measurement device, the following are provided within a shield frame 315 in which the interior is shielded from the outside when attached to a sensor sheet 100: a terminal (a spring probe 312) that is connected to a sensor (an electrode 133) of the sensor sheet 100; and an external terminal (a USB terminal 313) for connection to an external device.

Abstract: A terminal (300) is attached to a sensor sheet (100) using a tongue piece (131) that extends from the sensor sheet (100). This makes it possible to reduce a sense of discomfort in a subject caused by the presence of the terminal (300), and to realize a biological-information measurement device with which it is possible to mitigate a sense of discomfort in a subject caused by the device, to a greater extent than in a case in which, e.g., the entire reverse surface of the terminal (300) is secured to the sensor sheet (100).

Abstract: Provided is a sensor sheet 100 comprising: a lower sheet 110 that is affixed to the skin of a subject; an upper sheet 120 that is affixed to the surface side of the lower sheet 110; and an electric circuit section 130 provided to an area corresponding to the upper sheet 120. A peripheral edge section 110a has a single-layer structure comprising only the lower sheet 110. A central area has a multilayer structure comprising the lower sheet 110 and the upper sheet 120. In this way, the ability to conform to the skin is enhanced in the peripheral edge section 110a of the sheet where itching is likely to occur, the electric circuit section 130 can be held firmly in the central area.

Abstract: In this biological information measurement device, the following are provided within a shield frame 315 in which the interior is shielded from the outside when attached to a sensor sheet 100: a terminal (a spring probe 312) that is connected to a sensor (an electrode 133) of the sensor sheet 100; and an external terminal (a USB terminal 313) for connection to an external device.