Abstract: Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CET) family protein obtained by mutating the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles, and mutating the amino acid sequence of a C-terminal region of the cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.

Abstract: Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.

Abstract: Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.

Abstract: The present disclosure provides a method for producing a polyisoprenoid, which makes it possible to synthesize in vitro a polyisoprenoid having an unprecedented structure, such as a 100% cis-polyisoprenoid or a polyisoprenoid containing an allylic diphosphate derivative as an initiating terminal. The present disclosure relates to a method for producing a polyisoprenoid in vitro, which employs a gene coding for a neryl diphosphate synthase and rubber particles bound to a protein encoded by the gene, or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a gene coding for a neryl diphosphate synthase is linked to a promoter having a promoter activity that drives laticifer-specific gene expression to express a protein encoded by the gene specifically in laticifers.

Abstract: Objects are to provide: a fusion protein capable of binding to lipid droplets while having an enzymatic activity to synthesize a hydrophobic compound; a method for producing a substance including accumulating a hydrophobic compound in lipid droplets using the fusion protein; a vector which can enhance production of a hydrophobic compound when it is introduced into cells using genetic recombination techniques; and a transgenic cell into which the vector or a gene coding for the fusion protein has been introduced. The present disclosure relates to a fusion protein having an amino acid sequence (first amino acid sequence) capable of binding to lipid droplets, and an amino acid sequence (second amino acid sequence) having an enzymatic activity to synthesize a hydrophobic compound, with the enzymatic activity of the second amino acid sequence being maintained.

Abstract: Provided are tires that are excellent in tire properties such as fuel economy, abrasion resistance, wet grip performance, snow and ice performance, fracture resistance, and flex crack growth resistance. Included is a tire containing a rubber composition containing a natural rubber derived from Taraxacum kok-saghyz having a weight average molecular weight (Mw) of 5,000,000 or more and including polymers having a radius of gyration (Rz) of 130 nm or larger, wherein the Mw and Rz values are measured by FFF-MALS.

Abstract: Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.

Abstract: The present invention aims to provide a method for producing a trans-polyisoprenoid which can increase trans rubber production. The present invention is directed to a method for producing a trans-polyisoprenoid in vitro, which involves the use of a gene coding for a trans-prenyltransferase (tPT) family protein and further involves the use of rubber particles bound to a protein encoded by the gene, or a method for producing a trans-polyisoprenoid, which includes introducing into a plant a vector including a promotor having a promoter activity that drives laticifer-specific gene expression and a gene coding for a tPT family protein linked to the promotor to express a protein encoded by the gene specifically in laticifers.

Abstract: The present invention aims to provide a method for producing a trans-polyisoprenoid which can increase trans rubber production. The present invention is directed to a method for producing a trans-polyisoprenoid in vitro, which involves the use of a gene coding for a trans-prenyltransferase (tPT) family protein and further involves the use of rubber particles bound to a protein encoded by the gene, or a method for producing a trans-polyisoprenoid, which includes introducing into a plant a vector including a promotor having a promoter activity that drives laticifer-specific gene expression and a gene coding for a tPT family protein linked to the promotor to express a protein encoded by the gene specifically in laticifers.

Abstract: The present invention provides a method which can replace Agrobacterium techniques and which can efficiently produce transformed cells of a rubber-producing plant. The present invention relates to a method of producing transformed cells of a rubber-producing plant, which includes steps a) to e).

Abstract: Provided is a method that can efficiently produce a transgenic Taraxacum plant in a short period. A method of producing a transgenic Taraxacum plant, including: an infection step of infecting a tissue fragment from a Taraxacum plant with an Agrobacterium tumefaciens containing a plasmid containing a target gene or fragment thereof and hygromycin-resistance gene; a selective culture step of selecting the tissue fragment that has acquired the target gene from the tissue fragment obtained in the infection step by using hygromycin; a callus-inducing step of culturing the tissue fragment obtained in the selective culture step in a callus-inducing medium to form callus; a regeneration-inducing step of culturing the callus obtained in the callus-inducing step in a regeneration-inducing medium to form an adventitious embryo, an adventitious bud, and a shoot; and a rooting step of culturing the shoot obtained in the regeneration-inducing step in a rooting medium to root the shoot.

Abstract: The present invention provides a method for producing a polyisoprenoid with which it is possible to enhance the rubber synthesis activity of rubber particles to increase natural rubber production. The present invention relates to a method for producing a polyisoprenoid in vitro, which involves the use of a gene coding for a cis-prenyltransferase (CPT) family protein and a gene coding for a Nogo-B receptor (NgBR) family protein, and further involves the use of rubber particles bound to proteins encoded by these genes; or a method for producing a polyisoprenoid, which includes introducing into a plant a vector in which a promoter having a promoter activity that drives laticifer-specific gene expression is linked to a gene coding for a CPT family protein and a gene coding for a NgBR family protein, to express proteins encoded by the genes specifically in laticifers.

Abstract: The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.

Abstract: To provide methods for producing a natural rubber which enable the production of a natural rubber with an increased molecular weight, and transgenic plants which are able to produce a natural rubber with an increased molecular weight. Included is a method for producing a natural rubber, the method including the steps of: introducing a gene coding for a cis-prenyltransferase (CPT) family protein derived from a rubber-producing plant A into a rubber-producing plant B to prepare a transgenic plant C; and producing a natural rubber using the transgenic plant C, the rubber-producing plant A producing a natural rubber having a higher molecular weight than a natural rubber produced by the rubber-producing plant B not transfected with the gene.

Abstract: Provided are tires that are excellent in tire properties such as fuel economy, abrasion resistance, wet grip performance, snow and ice performance, fracture resistance, and flex crack growth resistance. Included is a tire containing a rubber composition containing a natural rubber derived from Taraxacum kok-saghyz having a weight average molecular weight (Mw) of 5,000,000 or more and including polymers having a radius of gyration (Rz) of 130 nm or larger, wherein the Mw and Rz values are measured by FFF-MALS.

Abstract: Provided is a mutant protein obtained by mutating a specific amino acid residue of HMGR, a rate-limiting enzyme of isoprene monomer biosynthesis in the polyisoprenoid biosynthesis pathway. The present invention relates to a mutant protein, wherein at least one amino acid residue selected from the group consisting of amino acid residues at positions 91, 225, 257, 287, 339, 411, 470, 509 and 574 of the Arabidopsis thaliana 3-hydroxy-3-methylglutaryl CoA reductase shown by SEQ ID NO:1 and amino acid residues at positions corresponding to the foregoing in 3-hydroxy-3-methylglutaryl CoA reductase is deleted or replaced with another amino acid residue.

Abstract: The present invention provides a method which can replace Agrobacterium techniques and which can efficiently produce transformed cells of a rubber-producing plant. The present invention relates to a method of producing transformed cells of a rubber-producing plant, which includes steps a) to e).

Abstract: Provided is a method that can efficiently produce a transgenic Taraxacum plant in a short period. A method of producing a transgenic Taraxacum plant, including: an infection step of infecting a tissue fragment from a Taraxacum plant with an Agrobacterium tumefaciens containing a plasmid containing a target gene or fragment thereof and hygromycin-resistance gene; a selective culture step of selecting the tissue fragment that has acquired the target gene from the tissue fragment obtained in the infection step by using hygromycin; a callus-inducing step of culturing the tissue fragment obtained in the selective culture step in a callus-inducing medium to form callus; a regeneration-inducing step of culturing the callus obtained in the callus-inducing step in a regeneration-inducing medium to form an adventitious embryo, an adventitious bud, and a shoot; and a rooting step of culturing the shoot obtained in the regeneration-inducing step in a rooting medium to root the shoot.

Abstract: The present invention aims to provide a method for producing a trans-polyisoprenoid which can increase trans rubber production. The present invention is directed to a method for producing a trans-polyisoprenoid in vitro, which involves the use of a gene coding for a trans-prenyltransferase (tPT) family protein and further involves the use of rubber particles bound to a protein encoded by the gene, or a method for producing a trans-polyisoprenoid, which includes introducing into a plant a vector including a promoter having a promoter activity that drives laticifer-specific gene expression and a gene coding for a tPT family protein linked to the promoter to express a protein encoded by the gene specifically in laticifers.

Abstract: Provided is a method for producing a polyisoprenoid, which can increase natural rubber production by enhancing the rubber synthesis activity of rubber particles. The present invention provides methods for producing a polyisoprenoid using a gene coding for a cis-prenyltransferase (CPT) family protein, a gene coding for a Nogo-B receptor (NgBR) family protein and a gene coding for a rubber elongation factor (REF) family protein, specifically a method for producing a polyisoprenoid in vitro using rubber particles bound to proteins coded for by these genes, and a method for producing a polyisoprenoid in vivo using a recombinant organism (plant) having these genes introduced therein.