Patents by Inventor Yusuke Fuchiwaki
Yusuke Fuchiwaki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240091771Abstract: An assay device and an assay method are each capable of ensuring the accuracy of a target substance detection section.Type: ApplicationFiled: September 17, 2021Publication date: March 21, 2024Inventors: Yusuke FUCHIWAKI, Masato TANAKA, Shohei YAMAMURA, Naoki MORISHITA, Kumiko KAMIYA, Seiichiro MATSUZAKI
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Patent number: 11781181Abstract: The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising: heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.Type: GrantFiled: February 5, 2020Date of Patent: October 10, 2023Assignees: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, KYORIN PHARMACEUTICAL CO., LTD.Inventors: Hidenori Nagai, Shunsuke Furutani, Yoshihisa Hagihara, Yusuke Fuchiwaki
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Patent number: 11098347Abstract: The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising: heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.Type: GrantFiled: July 7, 2015Date of Patent: August 24, 2021Assignees: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, KYORIN Pharmaceutical Co., Ltd.Inventors: Hidenori Nagai, Shunsuke Furutani, Yoshihisa Hagihara, Yusuke Fuchiwaki
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Publication number: 20210170398Abstract: An assay device allows enhancement of the liquid control performance. The assay device of the present invention includes a microflow passage 1,31,41 which allows flow of the liquid, an absorbing porous medium 2,42 disposed at a distance from one end of the microflow passage, and a separating space 3,43 disposed between the one end of the microflow passage and the absorbing porous medium. The assay device further includes two sideways ventilation passages 6,46 which are adjacent to both sides of the microflow passage, respectively in the width direction orthogonal to the flow direction, the two sideways ventilation passages 6,46 being communicated with the microflow passage to allow air circulation.Type: ApplicationFiled: August 29, 2019Publication date: June 10, 2021Inventor: Yusuke FUCHIWAKI
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Publication number: 20200157607Abstract: The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising: heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.Type: ApplicationFiled: February 5, 2020Publication date: May 21, 2020Applicants: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY, KYORIN PHARMACEUTICAL CO., LTD.Inventors: Hidenori NAGAI, Shunsuke FURUTANI, Yoshihisa HAGIHARA, Yusuke FUCHIWAKI
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Patent number: 10273532Abstract: The invention provides an ultra-rapid nucleic acid amplification method performed in a flow channel. Specifically, the invention provides a nucleic acid amplification method for performing a PCR reaction by supplying a PCR sample solution to a nucleic acid amplification device comprising a serpentine channel adapted to perform at least one PCR cycle, the nucleic acid amplification device comprising a DNA denaturation temperature zone corresponding to the curved portions at one side, an annealing temperature zone corresponding to the curved portions at the other side, and an extension temperature zone positioned between the annealing and DNA denaturation temperature zones, wherein the PCR sample solution is introduced in the form of sample plugs separated by gas into the serpentine channel using a pump, the sample solution being supplied into the channel in a state such that the solution is separated by gas into a segment corresponding to one PCR cycle or smaller segments.Type: GrantFiled: March 9, 2012Date of Patent: April 30, 2019Assignee: National Institute of Advanced Industrial Science and TechnologyInventors: Hidenori Nagai, Yusuke Fuchiwaki
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Patent number: 9744534Abstract: The assay device (10) comprises a micro flow path (76); a porous medium provided near the distal end portion (80) of the micro flow path (76); and a space (82) provided between the micro flow path (76) and the porous medium for controlling the flow rate of a fluid moving from the micro flow path (76) to the space (82). After a fluid moved along the micro flow path (76) based on a lateral flow is brought into contact with the porous medium beyond the space (82) and is absorbed to the porous medium, the fluid is divided by the space (82) so that the fluid stays in the micro flow path (76). With this structure, it is possible to perform solution exchange in the micro flow path without using an external device such as a pump.Type: GrantFiled: September 27, 2013Date of Patent: August 29, 2017Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Yusuke Fuchiwaki, Toshihiko Ooie, Masatoshi Kataoka, Hiroki Takaoka
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Publication number: 20170130261Abstract: The present invention provides a reciprocal-flow-type nucleic acid amplification device comprising: heaters capable of forming a denaturation temperature zone and an extension/annealing temperature zone; a fluorescence detector capable of detecting movement of a sample solution between the two temperature zones; a pair of liquid delivery mechanisms that allow the sample solution to move between the two temperature zones and that are configured to be open to atmospheric pressure when liquid delivery stops; a substrate on which the chip for nucleic acid amplification according to claim 2 can be placed; and a control mechanism that controls driving of each liquid delivery mechanism by receiving an electrical signal from the fluorescence detector relating to movement of the sample solution from the control mechanism; the device being capable of performing real-time PCR by measuring fluorescence intensity for each thermal cycle.Type: ApplicationFiled: July 7, 2015Publication date: May 11, 2017Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGYInventors: Hidenori NAGAI, Shunsuke FURUTANI, Yoshihisa HAGIHARA, Yusuke FUCHIWAKI
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Publication number: 20150266023Abstract: The assay device (10) comprises a micro flow path (76); a porous medium provided near the distal end portion (80) of the micro flow path (76); and a space (82) provided between the micro flow path (76) and the porous medium for controlling the flow rate of a fluid moving from the micro flow path (76) to the space (82). After a fluid moved along the micro flow path (76) based on a lateral flow is brought into contact with the porous medium beyond the space (82) and is absorbed to the porous medium, the fluid is divided by the space (82) so that the fluid stays in the micro flow path (76). With this structure, it is possible to perform solution exchange in the micro flow path without using an external device such as a pump.Type: ApplicationFiled: September 27, 2013Publication date: September 24, 2015Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE & TECHNOLOGYInventors: Yusuke Fuchiwaki, Toshihiko Ooie, Masatoshi Kataoka, Hiroki Takaoka
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Publication number: 20150031087Abstract: The invention provides an ultra-rapid nucleic acid amplification method performed in a flow channel. Specifically, the invention provides a nucleic acid amplification method for performing a PCR reaction by supplying a PCR sample solution to a nucleic acid amplification device comprising a serpentine channel adapted to perform at least one PCR cycle, the nucleic acid amplification device comprising a DNA denaturation temperature zone corresponding to the curved portions at one side, an annealing temperature zone corresponding to the curved portions at the other side, and an extension temperature zone positioned between the annealing and DNA denaturation temperature zones, wherein the PCR sample solution is introduced in the form of sample plugs separated by gas into the serpentine channel using a pump, the sample solution being supplied into the channel in a state such that the solution is separated by gas into a segment corresponding to one PCR cycle or smaller segments.Type: ApplicationFiled: March 9, 2012Publication date: January 29, 2015Applicant: National Institute of Advanced Industrial Science and TechnologyInventors: Hidenori Nagai, Yusuke Fuchiwaki