Abstract: Provided herein are hybrid capture-based methods to extract single-stranded DNA or RNA directly from non-treated biospecimens. The methods allow for the detection and analysis of unexplored short single-stranded DNA (sssDNA, mean length 50 nt) and ultrashort single-stranded DNA (ussDNA, mean length 15 nt) of human origin present in the biospecimen. The methods allow the discovery of unexplored short single-stranded DNA (sssDNA) in isolated red blood cells, which were believed to be deprived of nucleic acids because of the lack of a nucleus in mature red blood cells. The DNA or RNA extracted using the disclosed methods can be used as disease prognostic biomarkers and treatment predictive biomarkers.

Abstract: Provided herein are high-throughput methods for genetic barcoding and analysis, e.g., for tagging each biomaterial apsule with a barcode cell. These barcode cells are derived from patient samples, and thus embody natural human genetic variation. Also provided are SNP panels that can be used as genetic barcodes to identify the identity of a cell.

Abstract: Provided herein are compositions and methods for assembling multiple DNA molecules into a linear concatemer, with applications to nanopore sequencing of DNA sequence variations.

Abstract: A microangiography method and system based on full-space modulation spectrum splitting and angle compounding is disclosed. Label-free three-dimensional optical coherence tomography angiography is realized by combining the three-dimensional space resolution capability of an optical coherence tomography and the motion recognition capability of a dynamic scattering technology. Probe light of different incident angles is encoded with a transverse scanning modulation spectrum in a spatial frequency domain, incident angle-resolved sub-angiograms which are independent of one another are obtained by splitting the modulation spectrum, and an angiogram with multiple space angles compounded is realized. Conjugate mirror images are removed from a depth (z) domain, a complex-valued OCT interference spectra are reconstructed, the full-space modulation spectrum is obtained in the spatial frequency domain, and the overlap of the modulation spectrum conjugate mirror images is avoided.

Abstract: A cytocompatible hydrogel includes a plurality first polymer macromers linked to a plurality of second polymer macromers different than the first polymer macromers by a plurality of ?-hydroxythio-ether linkages, and a plurality of cells encapsulated in the hydrogel.

Abstract: A microangiography method and system based on full-space modulation spectrum splitting and angle compounding is disclosed. Label-free three-dimensional optical coherence tomography angiography is realized by combining the three-dimensional space resolution capability of an optical coherence tomography and the motion recognition capability of a dynamic scattering technology. Probe light of different incident angles is encoded with a transverse scanning modulation spectrum in a spatial frequency domain, incident angle-resolved sub-angiograms which are independent of one another are obtained by splitting the modulation spectrum, and an angiogram with multiple space angles compounded is realized. Conjugate mirror images are removed from a depth (z) domain, a complex-valued OCT interference spectra are reconstructed, the full-space modulation spectrum is obtained in the spatial frequency domain, and the overlap of the modulation spectrum conjugate mirror images is avoided.