Patents by Inventor Yuzuru Tozawa

Yuzuru Tozawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230374535
    Abstract: A method for producing an HSL protein with increased catalytic activity to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner is disclosed. A method for producing a plant with increased resistance to a 4-HPPD inhibitor using the method for producing the HSL protein is also disclosed. It has been revealed that, by mutating position 140 to a basic amino acid in an HSL protein, the catalytic activity of the protein to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner can be increased, and an activity of the protein to decompose the inhibitor can be increased.
    Type: Application
    Filed: July 7, 2023
    Publication date: November 23, 2023
    Applicants: National Agriculture and Food Research Organization, SDS BIOTECH K.K.
    Inventors: Yuzuru TOZAWA, Satomi Takei, Masahiro Oshima, Sakiko Hirose, Motoshige Kawata, Keisuke Sekino, Akihiko Yamazaki
  • Publication number: 20230279370
    Abstract: Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CET) family protein obtained by mutating the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles, and mutating the amino acid sequence of a C-terminal region of the cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
    Type: Application
    Filed: February 6, 2023
    Publication date: September 7, 2023
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY, NATIONAL UNIVERSITY CORPORATION SAITAMA UNIVERSITY
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Seiji TAKAHASHI, Toru NAKAYAMA, Satoshi YAMASHITA, Yuzuru TOZAWA
  • Publication number: 20230279371
    Abstract: Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a C-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
    Type: Application
    Filed: February 6, 2023
    Publication date: September 7, 2023
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY, NATIONAL UNIVERSITY CORPORATION SAITAMA UNIVERSITY
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Seiji TAKAHASHI, Toru NAKAYAMA, Satoshi YAMASHITA, Yuzuru TOZAWA
  • Publication number: 20230279369
    Abstract: Provided are a mutant cis-prenyltransferase (CPT) family protein and a method for producing a polyisoprenoid, which enable the production of a high molecular weight polyisoprenoid. Included is a mutant cis-prenyltransferase (CPT) family protein obtained by mutating the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein not found on rubber particles to be identical or similar to the amino acid sequence of a N-terminal region of a cis-prenyltransferase (CPT) family protein found on rubber particles.
    Type: Application
    Filed: February 6, 2023
    Publication date: September 7, 2023
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY, NATIONAL UNIVERSITY CORPORATION SAITAMA UNIVERSITY
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Seiji TAKAHASHI, Toru NAKAYAMA, Satoshi YAMASHITA, Yuzuru TOZAWA
  • Patent number: 11746357
    Abstract: In order to provide: a method for producing an HSL protein with increased catalytic activity to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner; and a method for producing a plant with increased resistance to a 4-HPPD inhibitor using the method for producing the HSL protein, it has been revealed that, by mutating position 140 to a basic amino acid in an HSL protein, the catalytic activity of the protein to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner can be increased, and an activity of the protein to decompose the inhibitor can be increased.
    Type: Grant
    Filed: February 9, 2018
    Date of Patent: September 5, 2023
    Assignees: National Agriculture and Food Research Organization, SDS BIOTECH K.K.
    Inventors: Yuzuru Tozawa, Satomi Takei, Masahiro Oshima, Sakiko Hirose, Motoshige Kawata, Keisuke Sekino, Akihiko Yamazaki
  • Publication number: 20230002792
    Abstract: Objects are to provide: a fusion protein capable of binding to lipid droplets while having an enzymatic activity to synthesize a hydrophobic compound; a method for producing a substance including accumulating a hydrophobic compound in lipid droplets using the fusion protein; a vector which can enhance production of a hydrophobic compound when it is introduced into cells using genetic recombination techniques; and a transgenic cell into which the vector or a gene coding for the fusion protein has been introduced. The present disclosure relates to a fusion protein having an amino acid sequence (first amino acid sequence) capable of binding to lipid droplets, and an amino acid sequence (second amino acid sequence) having an enzymatic activity to synthesize a hydrophobic compound, with the enzymatic activity of the second amino acid sequence being maintained.
    Type: Application
    Filed: October 27, 2020
    Publication date: January 5, 2023
    Applicants: Sumitomo Rubber Industries, Ltd., TOHOKU UNIVERSITY, KANAZAWA UNIVERSITY, National University Corporation Saitama University
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Seiji TAKAHASI, Toru NAKAYAMA, Satoshi YAMASHITA, Yuzuru TOZAWA
  • Patent number: 10889623
    Abstract: The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.
    Type: Grant
    Filed: May 31, 2016
    Date of Patent: January 12, 2021
    Assignees: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY
    Inventors: Haruhiko Yamaguchi, Yukino Inoue, Kazuhisa Fushihara, Seiji Takahashi, Satoshi Yamashita, Toru Nakayama, Yuzuru Tozawa
  • Publication number: 20200048315
    Abstract: In order to provide: a method for producing an HSL protein with increased catalytic activity to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner; and a method for producing a plant with increased resistance to a 4-HPPD inhibitor using the method for producing the HSL protein, it has been revealed that, by mutating position 140 to a basic amino acid in an HSL protein, the catalytic activity of the protein to oxidize a 4-HPPD inhibitor in a 2-oxoglutarate-dependent manner can be increased, and an activity of the protein to decompose the inhibitor can be increased.
    Type: Application
    Filed: February 9, 2018
    Publication date: February 13, 2020
    Applicants: National Agriculture and Food Research Organization, SDS Biotech K.K.
    Inventors: Yuzuru TOZAWA, Satomi TAKEI, Masahiro OSHIMA, Sakiko HIROSE, Motoshige KAWATA, Keisuke SEKINO, Akihiko YAMAZAKI
  • Publication number: 20180355000
    Abstract: The present invention provides a method for producing rubber particles bound to a membrane-associated protein by cell-free protein synthesis. The present invention relates to a method for producing rubber particles bound to a membrane-associated protein, the method including the step of performing protein synthesis in the presence of both rubber particles and a cell-free protein synthesis solution containing an mRNA coding for a membrane-associated protein to bind the membrane-associated protein to the rubber particles.
    Type: Application
    Filed: May 31, 2016
    Publication date: December 13, 2018
    Applicants: SUMITOMO RUBBER INDUSTRIES, LTD., TOHOKU UNIVERSITY
    Inventors: Haruhiko YAMAGUCHI, Yukino INOUE, Kazuhisa FUSHIHARA, Seiji TAKAHASHI, Satoshi YAMASHITA, Toru NAKAYAMA, Yuzuru TOZAWA
  • Publication number: 20080134370
    Abstract: A rice anthranilic acid synthase produced by introducing a variation in the base sequence of rice anthranilic acid synthase gene OASA2 so as to effect substitution for specified multiple amino acids. This synthase not only acquires a resistance to feedback inhibition by tryptophan but also retains an enzymatic activity identical with or higher than that of wild type rice anthranilic acid synthase.
    Type: Application
    Filed: October 11, 2005
    Publication date: June 5, 2008
    Inventors: Yuzuru Tozawa, Takuya Kanno, Kyo Wakasa
  • Patent number: 6388174
    Abstract: A DNA sequence is provided, which encodes an enzyme that is the &agr;-subunit of the first isozyme of rice anthranilate synthase (ASA). Transformed plants having a high tryptophan content can be regenerated by culturing the plant cells having introduced therein such a recombinant vector which contains, at a position downstream of a promoter, an inserted DNA fragment carrying the above DNA sequence. In addition, methods of selecting transformed plant cells are disclosed, wherein plant cells transformed with the discloses DNA are resistant to inhibitory concentrations of tryptophan or tryptophan analogues.
    Type: Grant
    Filed: April 25, 2000
    Date of Patent: May 14, 2002
    Assignees: Hokko Chemical Industry Co., Ltd., National Agricultural Research Organization (NARO)
    Inventors: Kyo Wakasa, Yuzuru Tozawa, Teruhiko Terakawa, Hisakazu Hasegawa