Abstract: The present invention provide a method for detecting the genome-wide off-target effects of adenine base editor (ABE) and the application in gene editing thereof. ABE comprises the TadA:TadA*:Cas9 fusion protein and gRNA which is able to catalyze the substitution of A to G with high efficiency at the target site, which can bring ABE a bright application prospect in gene editing and construction of disease model for human disease. Thus, the present invention provides the EndoV-seq method first time to detect the genome-wide off-target effects of ABE. The EndoV-seq method has a wide application prospect in gene editing, especially in gene editing for treatment field of human disease.

Abstract: The invention provides methods for rapidly determining kinase binding site motifs using a oriented degenerate peptide library approach. The methods involve the selection of peptides by binding affinity. Inhibitors of kinases that include or compete for the binding site motifs determined using the methods also are provided, as are methods and compositions for using these binding site motifs and inhibitors.

Abstract: Methods for determining an optimal binding motif for a binding compound are provided in which the binding compound is contacted with an oriented degenerate cyclic peptide library (ODCPL) under conditions which allow for interaction between the binding compound and the ODCPL such that a complex is formed between the binding compound and a subpopulation of library members capable of interacting with the binding compound. The subpopulation of library members capable of interacting with the binding compound is then separated from library members that are incapable of interacting with the binding compound. The subpopulation of library members capable of interacting with the binding compound is linearized to form a subpopulation of linearized library members.

Abstract: The invention provides a method for determining an amino acid sequence motif for a phosphorylation site of a protein kinase. In the method of the invention, a protein kinase is contacted with an oriented degenerate peptide library, peptides within the library which are substrates for the kinase are converted to phosphopeptides and the phosphopeptides are separated from non-phosphorylated peptides. The isolated phosphopeptides are sequenced and an amino acid sequence motif for the phosphorylation site is determined based upon the relative abundance of different amino acids residues at each degenerate position. The invention also provides peptide substrates for protein kinase A, cell cycle control kinases (including cyclin B/p33.sup.cdc2 and cyclin A/p33.sup.CDK2), src family kinases (including pp60.sup.c-src and pp60.sup.v-src), EGF receptor, p92.sup.

Abstract: The invention provides a method for determining an amino acid sequence motif for a phosphorylation site of a protein kinase. In the method of the invention, a protein kinase is contacted with an oriented degenerate peptide library, peptides within the library which are substrates for the kinase are converted to phosphopeptides and the phosphopeptides are separated from non-phosphorylated peptides. The isolated phosphopeptides are sequenced and an amino acid sequence motif for the phosphorylation site is determined based upon the relative abundance of different amino acids residues at each degenerate position. The invention also provides peptide substrates for protein kinase A, cell cycle control kinases, src family kinases, the EGF receptor and p92.sup.c-fps/fes based upon amino acid sequence motifs for the phosphorylation sites of these kinases.