Lipid Included In Apparatus Patents (Class 204/403.08)
  • Patent number: 10816558
    Abstract: An ascorbic acid responsive electrode comprising an electrode, and a detection layer comprising a non-catalytic electron acceptor that receives an electron from ascorbic acid, an amino acid, and a saccharide and/or a soluble protein; wherein in the detection layer the electron acceptor is reduced by the ascorbic acid, and the reduced electron acceptor is oxidized at the electrode.
    Type: Grant
    Filed: October 23, 2018
    Date of Patent: October 27, 2020
    Assignee: ARKRAY, Inc.
    Inventor: Kazuo Fukuda
  • Patent number: 10571396
    Abstract: The present invention is directed to multiplexed fluorescence detection, including time-resolved fluorescence (TRF) detection. A combination of spectral and temporal differences in fluorescence emission and spectral differences in excitation is used to enhance the ability to separate signals in an assay from multiple fluorescent labels. Different classes of labels may be utilized, including upconversion phosphors as well as lanthanide chelates and transition metal chelates. The methods may be implemented in optical plate readers, including cartridge-based multi-mode readers.
    Type: Grant
    Filed: February 25, 2016
    Date of Patent: February 25, 2020
    Assignee: Molecular Devices, LLC
    Inventors: Annegret Schramm, Michael Katzlinger
  • Patent number: 9903829
    Abstract: Electrodes (A, B) are provided in at least one of plate-like members corresponding to an inner side direction of supply path from an opening of supply path. Electrode (C) of a biological sample is provided in the inner side direction from electrodes (A, B) Reagent part is provided so as to cover electrodes (A, B) and electrode (C). Inflow restricting hole of a sample liquid is provided in at least one of plate-like members corresponding to the portions on both sides of supply path closer to the opening side than the electrode.
    Type: Grant
    Filed: April 12, 2012
    Date of Patent: February 27, 2018
    Assignee: PANASONIC HEALTHCARE HOLDINGS CO., LTD.
    Inventors: Hirofumi Ezaki, Mamiko Ochi, Akihisa Higashihara, Eriko Yoshioka
  • Patent number: 9797863
    Abstract: The invention features the use of graphene, a one atom thick planar sheet of bonded carbon atoms, in the formation of artificial lipid membranes. The invention also features the use of these membranes to detect the properties of polymers (e.g., the sequence of a nucleic acid) and identify transmembrane protein-interacting compounds.
    Type: Grant
    Filed: September 5, 2014
    Date of Patent: October 24, 2017
    Assignee: President and Fellows of Harvard College
    Inventors: Slaven Garaj, Daniel Branton
  • Patent number: 8354336
    Abstract: Accordingly, the present invention provides a method of forming an electrode having reduced corrosion and water decomposition on a surface thereof. A substrate which has a conductive layer disposed thereon is provided and the conductive layer has an oxide layer with an exposed surface. The exposed surface of the oxide layer contacts a solution of an organic surface active compound in an organic solvent to form a protective layer of the organic surface active compound over the oxide layer. The protective layer has a thickness of from about 0.5 nm to about 5 nm and ranges therebetween depending on a chemical structure of the surface active compound.
    Type: Grant
    Filed: June 22, 2010
    Date of Patent: January 15, 2013
    Assignee: International Business Machines Corporation
    Inventors: Ali Afzali-Ardakani, Shafaat Ahmed, Hariklia Deligianni, Dario L. Goldfarb, Stefan Harrer, Binquan Luan, Glenn J. Martyna, Hongbo Peng, Stanislav Polonsky, Stephen Rossnagel, Xiaoyan Shao, Gustavo A. Stolovitzky
  • Patent number: 8268143
    Abstract: The present invention provides an electrochemical sensor having a sensing chemistry that operates substantially free of any “oxygen effect”. The electrochemical sensors are useful in determining the level of an analyte in a biological sample from a subject. The present invention also provides sensor assemblies including the electrochemical sensors as well as methods of using the same.
    Type: Grant
    Filed: July 14, 2008
    Date of Patent: September 18, 2012
    Assignee: Abbott Diabetes Care Inc.
    Inventors: Zenghe Liu, Tianmei Ouyang, Hyun Cho, Yohannes Goti, Benjamin J. Feldman, Fei Mao, Adam Heller
  • Patent number: 8062491
    Abstract: A microelectromechanical systems (MEMS) and integrated circuit (IC) based biosensor capable of sensing or detecting various ionic molecules and macromelecules (DNA, RNA or protein). The MEMS based biosensor utilizes a hybridization and enzyme amplification scheme and an electrochemical detection scheme for sensitivity improvement and system miniaturization. The biosensor or biosensors are incorporated on a single substrate. Preferably, the biosensor system includes at least two electrodes. The electrodes includes a working electrode, a reference electrode and a counter (auxiliary) electrode. The biosensor or biosensors also provide an apparatus and method for confinement of reagent and/or solution in the biosensor or biosensors using surface tension at small scale. The confinement system provides controlled contacts between the reagent(s) and/or solution(s) with the components (i.e., electrodes) of the biosensor or biosensors using controllable surface properties and surface tension forces.
    Type: Grant
    Filed: June 20, 2008
    Date of Patent: November 22, 2011
    Assignee: The United States of America as represented by the Department of the Navy
    Inventor: Jen-Jr Gau
  • Patent number: 8062489
    Abstract: An object of the present invention is to provide a method for stably forming an artificial lipid membrane while suppressing the leakage and evaporation of an electrolytic solution. The present invention is an artificial lipid membrane forming method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The artificial lipid membrane forming apparatus comprises a first chamber, a second chamber, a dividing wall, and an artificial lipid membrane forming portion. Each of the first chamber and the second chamber has a capacity of not smaller than 10 pl and not larger than 200 ?l. The artificial lipid membrane forming method of the present invention comprises the steps of: preparing the artificial lipid membrane forming apparatus; adding to the first chamber a first electrolytic solution having a viscosity of not lower than 1.
    Type: Grant
    Filed: December 17, 2010
    Date of Patent: November 22, 2011
    Assignee: Panasonic Corporation
    Inventors: Akio Oki, Norihito Tsukahara, Masato Suzuki, Hiroaki Oka
  • Patent number: 7828947
    Abstract: An object of the present invention is to provide a method for easily forming an artificial lipid membrane in a short period of time and an artificial lipid membrane forming apparatus suitable for carrying out such method. The present invention relates to a method for forming an artificial lipid membrane using an artificial lipid membrane forming apparatus. The apparatus comprises a substrate, a first spacer, a first thin film, a second spacer, a second thin film, and a cover, wherein a first chamber is formed between the substrate and the first thin film, the first thin film has a first through hole, a second chamber is formed between the first thin film and the second thin film, the second thin film has a second through hole, and the cover has an inlet.
    Type: Grant
    Filed: May 3, 2010
    Date of Patent: November 9, 2010
    Assignee: Panasonic Corporation
    Inventors: Akio Oki, Hiroaki Oka
  • Patent number: 7410709
    Abstract: A bio-battery includes a biomolecular energy source, a first electrode and a second electrode. In some configurations, a bio-battery may also include a first cell containing the first electrode and the biomolecular energy source, and a second cell having a reducible substrate and the second electrode. The first cell can be in ionic communication with the second cell, for example by a proton exchange membrane. Various biomolecular energy sources can be used, including proton donor molecules or electrolytically oxidizable molecules. For example, the biomolecular energy source can be selected from the group consisting of Nicotinamide Adenine Dinucleotide (NADH), Nicotinamide Adenine Dinucleotide Phosphate (NADPH) and 5,10-Methylenetetrahydrofolate Reductase (FADH).
    Type: Grant
    Filed: June 24, 2004
    Date of Patent: August 12, 2008
    Assignee: Purdue Research Foundation
    Inventors: Michael R. Ladisch, Nathan S. Mosier, Eric M. Perkins
  • Patent number: 6863833
    Abstract: The invention provides microfabricated silicon substrates and devices having extremely small apertures (termed “nanoapertures”) and methods for producing such nanoapertures. The devices have a nanoaperture (which may have a diameter ranging from about a few millimeters to as small as a few nm) across a substrate effective to connect two regions separated by the substrate. The devices are suitable for the formation of lipid bilayer membranes across the apertures, and for use in devices such as biosensors. Substrates and devices may include multiple nanoapertures, which may each support a lipid bilayer membrane, allowing fault tolerant devices such as fault-tolerant biosensors, and allowing devices able to sense more than one target molecule.
    Type: Grant
    Filed: June 21, 2002
    Date of Patent: March 8, 2005
    Assignee: The Board of Trustees of the Leland Stanford Junior University
    Inventors: David M. Bloom, Mark C. Peterman, Jonathan M. Ziebarth
  • Patent number: 6861260
    Abstract: The object of the present invention is to provide a lipid membrane whose permeability to substances is high and strongly correlated with the permeability of biomembranes to drugs and which therefore is suitable for rapid measurement. The present invention provides a membrane comprising an unsaturated C7-C9 hydrocarbon and a lipid.
    Type: Grant
    Filed: March 23, 2001
    Date of Patent: March 1, 2005
    Assignee: Chugai Seiyaku Kabushiki Kaisha
    Inventor: Kiyohiko Sugano
  • Patent number: 6824659
    Abstract: A mutant staphylococcal alpha hemolysin polypeptide containing a heterologous analyte-binding amino acid which assembles into an analyte-responsive heptameric pore assembly in the presence of a wild type staphylococcal alpha hemolysin polypeptide, digital biosensors, and methods of detecting, identifying, and quantifying analytes are described.
    Type: Grant
    Filed: February 15, 2001
    Date of Patent: November 30, 2004
    Assignee: University of Massachusetts
    Inventors: Hagan Bayley, Orit Braha, John Kasianowicz, Eric Gouaux
  • Patent number: 6740213
    Abstract: The present invention provides a biosensor comprising an electrode and a membrane in which the biosensor includes at least two zones each zone differing from each other zone in a property. The membrane includes a plurality of ionophores, at least a proportion of which are capable of lateral diffusion within the membrane. A plurality of first binding partner molecules are attached to membrane elements positioned within a first zone such that the first binding partner molecules are prevented from diffusing laterally into a second zone. Second binding partner molecules are attached to the ionophores and the rate of lateral diffusion within the membrane of the first binding partner molecules and second binding partner molecules is different.
    Type: Grant
    Filed: January 16, 2001
    Date of Patent: May 25, 2004
    Assignees: Australian Membrane and Biotechnology Research Institute, The University of Sydney
    Inventors: Peter Damien John Osman, Maxwell John Crossley, Alastair Scott Martin, Ronald John Pace
  • Patent number: 6478939
    Abstract: A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an &agr;-helical coiled-coil heterodimer. The first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.
    Type: Grant
    Filed: May 24, 2000
    Date of Patent: November 12, 2002
    Assignees: Pence, Inc., McGill University
    Inventors: R. Bruce Lennox, Robert S. Hodges, Randall T. Irvin
  • Patent number: 6461490
    Abstract: A biosensor apparatus for detecting a binding event between a ligand and receptor. The apparatus includes a biosensor surface and surface-bound two-subunit heterodimer complexes composed of first and second, preferably oppositely charged peptides that together form an &agr;-helical coiled-coil heterodimer. The-first peptide is attached to the biosensor surface, and the second peptide carries the ligand, accessible for binding by a ligand-binding agent. Binding of anti-ligand binding agent to the surface-bound ligand is detected by a suitable detector. A ligand-specific biosensor surface can be readily prepared from a universal template containing the first charged peptide, by addition of a selected ligand attached to the second peptide.
    Type: Grant
    Filed: July 27, 2000
    Date of Patent: October 8, 2002
    Assignees: PENCE, Inc., McGill University
    Inventors: R. Bruce Lennox, Robert S. Hodges, Randall T. Irvin
  • Patent number: 6451196
    Abstract: An improved membrane based biosensor incorporates sensing and reference electrodes and a dc electrical potential produced by a counter electrode. The biosensor incorporates ionophores. The conductivity of the membrane is dependent on the presence or absence of an analyte. A functional reservoir exists between the sensing electrode and a lipid membrane deposited on the sensing electrode. The invention also includes the method of detecting the presence or absence of the analyte by use of the biosensor.
    Type: Grant
    Filed: January 4, 1999
    Date of Patent: September 17, 2002
    Assignees: Australian Membrane and Biotechnology Research Institute, The University of Sydney
    Inventors: Peter Damien John Osman, Burkhard Raguse, Lech Wieczorek