Dielectrophoretic (i.e., Uses Nonuniform Electric Field) Patents (Class 204/643)
  • Patent number: 10272428
    Abstract: A microfluidic device can include: a channel; a fluid inlet to pass fluid into the channel from a reservoir; a sensor disposed in the channel; and a pump actuator disposed in the channel apart from the sensor to induce fluid flow into the channel.
    Type: Grant
    Filed: April 30, 2013
    Date of Patent: April 30, 2019
    Assignee: Hewlett-Packard Development Company, L.P.
    Inventors: Nicholas M. McGuinness, Manish Giri, Melinda M. Valencia, Chantelle Domingue, Andrew L. Van Brocklin, Dustin W. Blair
  • Patent number: 10241343
    Abstract: A 3D conversion lens and a control method thereof are disclosed. The 3D conversion lens has a first substrate and a second substrate, the first substrate and the second substrate forming a box; electronic ink, the electronic ink filling in the box cavity of the box, and the electronic ink has opaque charged particles, the opaque charged particles have opaque pigment; an electrode formed on the first substrate; and an electrode formed on at least one side wall of the box cavity. With the conventional liquid crystal layer replaced by electronic ink, 3D display effect of the glasses is realized by controlling positive and negative polarity of the transparent electrodes on the substrate and the side wall of the box cavity. In this way, it is unnecessary to control and realize deflection of the liquid crystals, which simplifies the circuit structure and the preparation process of the 3D glasses.
    Type: Grant
    Filed: August 29, 2014
    Date of Patent: March 26, 2019
    Assignees: BOE TECHNOLOGY GROUP CO., LTD., BEIJING BOE DISPLAY TECHNOLOGY CO., LTD.
    Inventors: Chunbing Zhang, Xin Gai
  • Patent number: 10222392
    Abstract: A continuous throughput microfluidic system includes an input system configured to provide a sequential stream of sample plugs; a droplet generator arranged in fluid connection with the input system to receive the sequential stream of sample plugs and configured to provide an output stream of droplets; a droplet treatment system arranged in fluid connection with the droplet generator to receive the output stream of droplets in a sequential order and configured to provide a stream of treated droplets in the sequential order; a detection system arranged to obtain detection signals from the treated droplets in the sequential order; a control system configured to communicate with the input system, the droplet generator, and the droplet treatment system; and a data processing and storage system configured to communicate with the control system and the detection system.
    Type: Grant
    Filed: May 4, 2016
    Date of Patent: March 5, 2019
    Assignees: The Johns Hopkins University, Pioneer Hi-Bred International, Inc.
    Inventors: Tza-huei J. Wang, Tushar Dnyandeo Rane, Helena Claire Zec, Wen-Chy Chu
  • Patent number: 9976983
    Abstract: A semiconductor arrangement and method of formation are provided. The semiconductor arrangement includes an electro-wetting-on-dielectric (EWOD) device. The EWOD device includes a top portion over a bottom portion and a channel gap between the top portion and the bottom portion. The bottom portion includes a driving dielectric layer over a first electrode, a second electrode and a first separating portion of an ILD layer between the first electrode and a second electrode. The driving dielectric layer has a first thickness less than about 1,000 ?. An EWOD device with a driving dielectric layer having a first thickness less 1000 ? requires a lower applied voltage to alter a shape of a droplet within the device and has a longer operating life than an EWOD device that requires a higher applied voltage to alter the shape of the droplet.
    Type: Grant
    Filed: March 7, 2014
    Date of Patent: May 22, 2018
    Assignee: Taiwan Semiconductor Manufacturing Company Limited
    Inventors: Yi-Hsien Chang, Chun-Ren Cheng
  • Patent number: 9808800
    Abstract: According to one aspect of the present disclosure, a control-engaged electrode-driving method for droplet actuation is provided. The method includes, a first voltage is provided to a first electrode for licking off a droplet. A second voltage is naturally discharged to a third voltage for maintaining a droplet movement. A fourth voltage is provided to the first electrode for accelerating the droplet. Naturally discharging from the second voltage to the third voltage and providing the fourth voltage to the first electrode are repeated. The first voltage is provided to a second electrode when a centroid of the droplet reaching a centroid of the first electrode. Naturally discharging from the second voltage to the third voltage and providing the fourth voltage to the second electrode are repeated.
    Type: Grant
    Filed: April 10, 2015
    Date of Patent: November 7, 2017
    Assignee: UNVERSITY OF MACAU
    Inventors: Tianlan Chen, Cheng Dong, Jie Gao, Yanwei Jia, Pui-In Mak, Mang-I Vai, Rui Paulo da Silva Martins
  • Patent number: 9387488
    Abstract: Methods, structures, devices and systems are disclosed for rapid enrichment and mass transport of biomolecules (e.g., such as proteins) or other small molecules and particles using electrodeless dielectrophoresis (eDEP). In one aspect, a device to aggregate molecules includes a substrate that is electrically insulating, an electrically insulative material formed on the substrate and structured to form a channel to carry an electrically conducting fluid containing particles, a constriction structure formed of the electrically insulative material and located in the channel to narrow a channel dimension and forming an opening with a size in the nanometer range, and a circuit coupled to the substrate to apply an ac electric field and a dc bias electric field along the channel, in which the constriction structure is structured to magnify the applied ac electric field to produce forces that operate collectively to aggregate the particles.
    Type: Grant
    Filed: November 13, 2012
    Date of Patent: July 12, 2016
    Assignee: ACADEMIA SINICA
    Inventors: Chia-Fu Chou, Kuo-Tang Liao
  • Patent number: 9358551
    Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.
    Type: Grant
    Filed: October 30, 2015
    Date of Patent: June 7, 2016
    Assignee: ADVANCED LIQUID LOGIC, INC.
    Inventors: Prasanna Thwar, Vamsee K. Pamula, Arjun Sudarsan, Ramakrishna Sista
  • Patent number: 9360402
    Abstract: Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. In one embodiment, an ionic propulsion device for providing a sample for a bio-specific assay of aerosol particles comprises a housing receiving a sample of aerosol particles and enclosing a high voltage electrode to generate a plasma of electrically charged particles. A carrier assembly is removably receivable in the housing, the carrier assembly comprising a non-conductive carrier and an electrode removably secured to the carrier.
    Type: Grant
    Filed: August 22, 2014
    Date of Patent: June 7, 2016
    Assignee: Inspirotec, Inc.
    Inventor: Julian Gordon
  • Patent number: 9144806
    Abstract: An optically-induced dielectrophoresis device includes a first substrate, a first conductive layer, a first patterned photoconductor layer, a first patterned layer, a second substrate, a second conductive layer, and a spacer. The first conductive layer is disposed on the first substrate. The first patterned photoconductor layer is disposed on the first conductive layer. The first patterned layer is disposed on the first conductive layer. The first patterned photoconductor layer and the first patterned layer are distributed alternately over the first conductive layer. Resistivity of the first patterned photoconductor layer is not equal to resistivity of the first patterned layer. At least one of the first substrate and the second substrate is pervious to a light. The second conductive layer is disposed on the second substrate and between the first substrate and the second substrate. The spacer connects the first substrate and the second substrate.
    Type: Grant
    Filed: July 4, 2013
    Date of Patent: September 29, 2015
    Assignee: Industrial Technology Research Institute
    Inventors: Hsiu-Hsiang Chen, Hsin-Hsiang Lo, Chun-Chuan Lin, Chi-Shen Chang, Jyh-Chern Chen, Kuo-Tung Tiao, Kuo-Yao Weng
  • Patent number: 9039883
    Abstract: A well, in particular an open well (14) with an upper end having a vertical axis (101), for containing a liquid and particles contained within said liquid, characterized by comprising at least two manipulation electrodes (1, 2, 3, 31, 32, 36, 17, 40, 41) able to be powered by electrical voltages, in particular alternating electrical voltages, so as to manoeuvre particles within the well by means of the dielectrophoretic effect. A platform comprising a plurality of wells as described above and a method for using said well.
    Type: Grant
    Filed: May 31, 2007
    Date of Patent: May 26, 2015
    Assignee: CELLPLY S.R.L.
    Inventors: Roberto Guerrieri, Massimo Bocchi
  • Publication number: 20150136607
    Abstract: Methods and apparatus are described for the processing (for example washing, incubation, etc.
    Type: Application
    Filed: December 19, 2014
    Publication date: May 21, 2015
    Inventors: Nicolò Manaresi, Roberto Guerrieri, Gianni Medoro
  • Patent number: 9034162
    Abstract: A microfluidic cell for the dielectrophoretic separation, accumulation, and/or lysis of polarizable bioparticles, including an interdigital electrode system composed of two electrode groups having interdigitated electrodes, and a micromixer having microchannels and microelevations. The interdigital electrode system and the micromixer are situated on the same side of the cell to improve the separation, accumulation, and/or lysis characteristics. Moreover, also described is a microfluidic system which includes such a microfluidic cell, and use thereof, and a method for separating, accumulating, and/or lysing polarizable bioparticles.
    Type: Grant
    Filed: August 10, 2010
    Date of Patent: May 19, 2015
    Assignee: ROBERT BOSCH GMBH
    Inventors: Michael Stumber, Martina Daub, Jochen Rupp, Massimo Kubon, Peter Rothacher, Meike Moschallski, Martin Stelzle, Christian Dorrer
  • Publication number: 20150122653
    Abstract: The invention relates to a method for the separation of a polarisable bioparticle comprising the steps: a) dielectrophoretic preseparation of a polarisable bioparticle from a suspension of bioparticles; b) fluidic separation of the selected bioparticle by fixing the bioparticle in a dielectrophoretic field cage and circulating fluid around the bioparticle; c) transferring the separated bioparticle from the dielectrophoretic field cage to a culture chamber; d) dielectrophoretic fixing of the separated bioparticle in the culture chamber and study, observation, manipulation and/or culturing of the separated bioparticle. The invention further relates to a microfluidic system and use thereof.
    Type: Application
    Filed: May 9, 2012
    Publication date: May 7, 2015
    Applicant: Technische Universitat Dortmund
    Inventors: Andreas Schmid, Lars M. Blank, Steffen Howitz, Frederik Sven Ole Fritzsch
  • Patent number: 8998483
    Abstract: A method of manipulating particles suspended within a fluid droplet using a microfluidic system including a piezo-electric substrate (1) and a wave generation means (3) for generating a wave within the piezoelectric substrate (1), and a working surface (2) through which the wave can be distributed and upon which fluid droplets (9) can be located, the method including locating one or more droplets of fluid on the working surface (2), varying the power applied to the wave generation means (3) or varying the distribution of the wave across the working surface (2), such that particles (1 1) suspended within the fluid droplet (9) are either dispersed within the droplet or concentrated in an area within the droplet in dependence on the power or wave distribution applied by the wave generation means (3) to the piezoelectric substrate (1), or to facilitate rotation of the fluid within said fluid droplets (9) located jn the path of the wave.
    Type: Grant
    Filed: May 2, 2007
    Date of Patent: April 7, 2015
    Assignee: Royal Melbourne Institute Technology
    Inventors: James Robert Friend, Leslie Yu-Ming Yeo
  • Publication number: 20150090592
    Abstract: Embodiments of an improved sensor for dielectrophoretic cytometry are presented. In one embodiment, the sensor includes a plurality of sensor electrodes as well as an actuation electrode. Embodiments of microfluidic systems incorporating such sensor are also described. Additionally, embodiments of methods or performing cytometry analysis are also presented.
    Type: Application
    Filed: October 6, 2014
    Publication date: April 2, 2015
    Inventors: Douglas Thomson, Gregory Bridges, Michael Butler, Marija Nikolic-Jaric, Elham Salimi, Tim Cabel, Szymon Rzeszowski, Graham Ferrier, Katrin Braasch
  • Patent number: 8992754
    Abstract: The present invention relates to a method and an apparatus for the characterization and/or the counting of particles by means of non uniform, time variable force fields and integrated optical or impedance meter sensors. The force fields can be of positive or negative dielectrophoresis, electrophoresis or electro-hydrodynamic motions, characterized by a set of stable equilibrium points for the particles (solid, liquid or gaseous); the same method is suitable for the manipulation of droplets (liquid particles) by exploiting effects known to the international scientific community with the name of Electro-wetting on dielectric.
    Type: Grant
    Filed: February 20, 2014
    Date of Patent: March 31, 2015
    Assignee: Silicon Biosystems S.p.A.
    Inventors: Nicolò Manaresi, Roberto Guerrieri, Gianni Medoro
  • Publication number: 20150068903
    Abstract: The invention provides droplet actuators with droplet operations surfaces for manipulating droplets, e.g., by conducting droplet operations. The droplet operations surfaces are typically exposed to a droplet operations gap. One or more regions of a droplet operation surface may include patterned topographic features. The invention also provides a droplet actuator in which one or both gap-facing droplet operations surfaces is formed using a removable film. The removable film may, in various embodiments, also include other components ordinarily associated with the droplet actuator substrate, such as the dielectric layer and the electrodes. Further, the invention provides droplet actuator devices and methods for coupling and/or sealing substrates of a droplet actuator, such as techniques for self-aligning assembly of droplet actuator substrates.
    Type: Application
    Filed: September 26, 2014
    Publication date: March 12, 2015
    Applicant: ADVANCED LIQUID LOGIC, INC.
    Inventors: Vijay Srinivasan, Arjun Sudarsan, Vamsee K. Pamula, Michael G. Pollack, Ramakrishna Sista, Raymond Kozikowski, Nicole Bell
  • Patent number: 8974652
    Abstract: Disclosed are apparatuses, systems, and methods for programmable fluidic processors. In one embodiment, the invention involves manipulating droplets across a reaction surface of the processor substantially contact-free of any surfaces. The reaction surface and the electrodes of the processor may include a coating repelling the droplets. Further, the present invention provides for a suitable suspending medium for repelling droplets away from fixed surfaces.
    Type: Grant
    Filed: May 27, 2005
    Date of Patent: March 10, 2015
    Assignee: Board of Regents, The University of Texas System
    Inventors: Peter R. C. Gascoyne, Jody Vykoukal, Jon Schwartz
  • Publication number: 20150060284
    Abstract: Apparatuses and methods for manipulating droplets are disclosed. In one embodiment, an apparatus for manipulating droplets is provided, the apparatus including a substrate, multiple arrays of electrodes disposed on the substrate, wherein corresponding electrodes in each array are connected to a common electrical signal, and a dielectric layer disposed on the substrate first side surface and patterned to cover the electrodes.
    Type: Application
    Filed: November 11, 2014
    Publication date: March 5, 2015
    Applicant: DUKE UNIVERSITY
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Richard B. Fair
  • Patent number: 8968543
    Abstract: The present invention provides an apparatus and a method to assemble organotypical tissues which can then be perfused and investigated under preferably physiological conditions.
    Type: Grant
    Filed: March 22, 2012
    Date of Patent: March 3, 2015
    Assignee: NMI Naturwissenschaftliches und Medizinisches Institut an der Universitaet Tuebingen
    Inventors: Martin Stelzle, Brigitte Angres, Massimo Kubon, Julia Schuette, Britta Hagmeyer, Felix Holzner
  • Patent number: 8968542
    Abstract: Devices and methods for performing dielectrophoresis are described. The devices contain sample channel which is separated by physical barriers from electrode channels which receive electrodes. The devices and methods may be used for the separation and analysis of particles in solution, including the separation and isolation of cells of a specific type. As the electrodes do not make contact with the sample, electrode fouling is avoided and sample integrity is better maintained.
    Type: Grant
    Filed: March 9, 2010
    Date of Patent: March 3, 2015
    Assignee: Virginia Tech Intellectual Properties, Inc.
    Inventors: Rafael V. Davalos, Hadi Shafiee, Michael B. Sano, John L. Caldwell
  • Publication number: 20150056638
    Abstract: The present invention provides devices and methods to separate and concentrate target protein species at a microliter scale and to generate reagents to those variants with exquisite selectivity for specific protein isoforms using only picograms of target material.
    Type: Application
    Filed: March 12, 2013
    Publication date: February 26, 2015
    Inventors: Michael Sierks, Mark Hayes
  • Publication number: 20150041325
    Abstract: A 3-dimensional PDMS cell sorter having multiple passages in a PDMS layer that follow the same path in a DEP separation region and that are in fluid communication with each other within that region. The passages may differ in width transverse to the flow direction within the passages. Flat plates may sandwich the PDMS layer; each plate may have a planar electrode used to generate a DEP field within a sample fluid flowed within the passages. The DEP field may concentrate target cells or particulates within one of the passages within the DEP separation region. The passages may diverge after the DEP-separation region, leaving one passage with a high concentration of target cells or particulates. Techniques for manufacturing such structures, as well as other micro-fluidic structures, are also provided.
    Type: Application
    Filed: March 27, 2013
    Publication date: February 12, 2015
    Inventors: Pei-yu E. Chiou, Kuo-wei Huang, Yu-jui Fan, Yu-chun Kung
  • Publication number: 20150027895
    Abstract: A method and apparatus for microfluidic processing by programmably manipulating a packet. A material is introduced onto a reaction surface and compartmentalized to form a packet. A position of the packet is sensed with a position sensor. A programmable manipulation force is applied to the packet at the position. The programmable manipulation force is adjustable according to packet position by a controller. The packet is programmably moved according to the programmable manipulation force along arbitrarily chosen paths.
    Type: Application
    Filed: August 5, 2014
    Publication date: January 29, 2015
    Inventors: Frederick F. BECKER, Peter GASCOYNE, Xiaobo WANG, Jody Valentine VYKOUKAL, Giovanni De GASPERIS
  • Patent number: 8940148
    Abstract: The present invention provides a nano-fluidic field effective device. The device includes a channel having a first side and a second side, a first set of electrodes adjacent to the first side, a second set of electrodes adjacent to the second side, a control unit for applying electric potentials to the electrodes and a fluid within the channel containing a charge molecule. The first set of electrodes is disposed such that application of electric potentials produces a spatially varying electric field that confines a charged molecule within a predetermined area of said channel. The second set of electrodes is disposed such that application of electric potentials relative to the electric potentials applied to the first set of electrodes creates an electric field that confines the charged molecule to an area away from the second side of the channel.
    Type: Grant
    Filed: June 22, 2010
    Date of Patent: January 27, 2015
    Assignee: International Business Machines Corporation
    Inventors: Ali Afzali-Ardakani, Stefan Harrer, Binquan Luan, Glenn J. Martyna, Dennis M. Newns, Hongbo Peng, Stanislav Polonsky, Stephen Rossnagel, Gustavo Stolovitzky
  • Publication number: 20150021189
    Abstract: A droplet actuator configured to improve the throughput of droplet operations in a detection spot of the droplet actuator and/or to reduce carryover problems is provided. The droplet actuator may include electrodes configured for effecting droplet operations transporting droplets on a surface; a sensor arranged in proximity to one or more of the electrodes establishing a detection window on the surface for detection of one or more properties of one or more droplets on the surface; wherein the electrodes may establish at least two pathways for transport of droplets into the detection window.
    Type: Application
    Filed: August 8, 2014
    Publication date: January 22, 2015
    Applicant: ADVANCED LIQUID LOGIC, INC.
    Inventors: Vijay Srinivasan, Vamsee K. Pamula, Ramakrishna Sista, Michael G. Pollack
  • Publication number: 20150014171
    Abstract: A microfluidic particle separation device includes a substrate and a plurality of electrode bars formed on the substrate, disposed around as array center, angularly spaced apart from one another, and extending radially with respect to the array center so as to form a radially-extending electrode array that is capable of inducing circular or elliptical shear flow of the liquid through travelling-wave electroosmosis when being applied with a travelling-wave electric potential.
    Type: Application
    Filed: April 29, 2014
    Publication date: January 15, 2015
    Applicant: National Taiwan University
    Inventors: Shiang-Chi LIN, Chih-Ting LIN, Yi-Chung TUNG, Yu-Lung SUNG
  • Patent number: 8932447
    Abstract: Devices and techniques are described that involve a combination of multidimensional electrokinetic, dielectrophoretic, electrophoretic and fluidic forces and effects for separating cells, nanovesicles, nanoparticulates and biomarkers (DNA, RNA, antibodies, proteins) in high conductance (ionic) strength biological samples and buffers. In disclosed embodiments, a combination of continuous and/or pulsed dielectrophoretic (DEP) forces, continuous and/or pulsed field DC electrophoretic forces, microelectrophoresis and controlled fluidics are utilized with arrays of electrodes. In particular, the use of chambered DEP devices and of a properly scaled relatively larger electrode array devices that combines fluid, electrophoretic and DEP forces enables both larger and/or clinically relevant volumes of blood, serum, plasma or other samples to be more directly, rapidly and efficiently analyzed. The invention enables the creation of “seamless” sample-to-answer diagnostic systems and devices.
    Type: Grant
    Filed: October 25, 2013
    Date of Patent: January 13, 2015
    Assignee: The Regents of the University of California
    Inventors: Michael Heller, Benjamin Sullivan, Rajaram Krishnan, Dennis Carson, Sadik C. Esener
  • Publication number: 20150008127
    Abstract: An apparatus and method for measurement are disclosed. The apparatus and method separate solutes and suspended solids in a mixture using a dielectrophoretic force provided by an electrode and a conductive layer, and then perform quantitative or qualitative analysis on at least one of the solutes using the electrode or at least one of the suspended solids.
    Type: Application
    Filed: July 3, 2014
    Publication date: January 8, 2015
    Inventors: Shu-Hsien Liao, Hsien Chang Chang
  • Publication number: 20150001081
    Abstract: A method for enriching a heterogeneous population of cells includes loading one or more sample chambers containing DEP electrodes therein with a solution containing the heterogeneous population of cells, wherein the heterogeneous population of cells comprises a first subpopulation of cells having a first crossover frequency and a second subpopulation of cells having a second, higher crossover frequency. An AC electrical field is applied to the DEP electrodes, wherein the AC electrical field has an applied frequency that is between the crossover frequency of the first subpopulation of cells and the second subpopulation of cells, wherein the first subpopulation of cells are substantially killed by the applied electrical field and the second subpopulation of cells are substantially not killed by the applied electrical field.
    Type: Application
    Filed: June 27, 2014
    Publication date: January 1, 2015
    Inventors: Jente Lu, Lisa A. Flanagan, Abraham P. Lee
  • Publication number: 20140367260
    Abstract: An apparatus for identifying objects in a plurality of objects includes a portion which applies dielectrophoresis to the plurality of objects. The apparatus includes a portion which tracks the plurality of objects' reaction to the dielectrophoresis over time and extracts visible features about the plurality objects being tracked. The apparatus includes a portion which automatically identifies the objects from the plurality of objects based on the objects' reaction to the dielectrophoresis over time and the visible features of the objects. A method for identifying objects in a plurality of objects. A dielectrophoresis cartridge.
    Type: Application
    Filed: June 4, 2014
    Publication date: December 18, 2014
    Applicant: NANOPHORETICS LLC
    Inventors: Samuel J. Dickerson, Donald M. Chiarulli, Steven P. Levitan, Craig Carthel, Stefano Coraluppi
  • Publication number: 20140371102
    Abstract: Disclosed are embodiments of methods, apparatus, systems, compositions, and articles of manufacture relating to identifying the source of bioparticles, such as bioparticles shed by an organism. In embodiments, a method may include collecting a sample of bioparticles from the environment, selecting from that sample the bioparticles most informative for identifying their source, and gathering data from those bioparticles to form bioparticle signatures; the bioparticle signatures may be processed into a multi-dimensional vector which may be compared to a multi-dimensional vector derived from a standard using a pattern recognition strategy. In embodiments, an apparatus may include a particle collection device to collect a sample, a transfer device to select bioparticles, and a detector that restricts the movement of the bioparticles; the restricted movement may be used to produce a bioparticle signature.
    Type: Application
    Filed: September 1, 2014
    Publication date: December 18, 2014
    Inventors: Mark A. Hayes, Thomas J. Taylor
  • Patent number: 8911606
    Abstract: Disclosed herein are methods and devices for dielectrokinetic chromatography. As disclosed, the devices comprise microchannels having at least one perturber which produces a non-uniformity in a field spanning the width of the microchannel. The interaction of the field non-uniformity with a perturber produces a secondary flow which competes with a primary flow. By decreasing the size of the perturber the secondary flow becomes significant for particles/analytes in the nanometer-size range. Depending on the nature of a particle/analyte present in the fluid and its interaction with the primary flow and the secondary flow, the analyte may be retained or redirected. The composition of the primary flow can be varied to affect the magnitude of primary and/or secondary flows on the particles/analytes and thereby separate and concentrate it from other particles/analytes.
    Type: Grant
    Filed: October 10, 2013
    Date of Patent: December 16, 2014
    Assignee: Sandia Corporation
    Inventors: Gabriela S. Chirica, Gregory J. Fiechtner, Anup K. Singh
  • Patent number: 8900434
    Abstract: A dielectrophoresis method for separating particles from a sample, including a dielectrophoresis channel, the dielectrophoresis channel having a central axis, a bottom, a top, a first side, and a second side; a first mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the first mesa extending at an angle to the central axis of the dielectrophoresis channel; a second mesa projecting into the dielectrophoresis channel from the bottom and extending from the first side across the dielectrophoresis channel to the second side, the second mesa parallel to said first mesa; a space between at least one of the first electrode and the second side or the second electrode and the second side; and a gap between the first electrode and the second electrode, and pumping a recovery fluid through said gap between said first electrode and into said space between at least one of said first mesa and said second side or said s
    Type: Grant
    Filed: July 18, 2013
    Date of Patent: December 2, 2014
    Assignee: Lawrence Livermore National Security, LLC.
    Inventors: Dietrich A. Dehlinger, Klint A. Rose, Maxim Shusteff, Christopher G. Bailey, Raymond P. Mariella, Jr.
  • Publication number: 20140346045
    Abstract: A continuous flow particle separation system for separating metallic and nonmetallic particles from a mixed-particle suspension includes a fluid channeling component defining an input channel and first and second output channels fluidly connected to the input channel at a bifurcated junction, a first electrode and a second electrode arranged proximate the input channel at least partially prior to the bifurcated junction, and an alternating current (AC) electric power source electrically connected to the first and second electrodes.
    Type: Application
    Filed: March 19, 2014
    Publication date: November 27, 2014
    Applicant: The Johns Hopkins University
    Inventors: Su Chih Chi, Robert Cammarata, Stephen L. Farias, Donglei Fan, Danru Qu, Chia-Ling Chien
  • Publication number: 20140346044
    Abstract: A continuous flow particle separation system for separating metallic and nonmetallic particles from a mixed-particle suspension includes a fluid channeling component defining an input channel and first and second output channels fluidly connected to the input channel at a bifurcated junction, a first electrode and a second electrode arranged proximate the input channel at least partially prior to the bifurcated junction, and an alternating current (AC) electric power source electrically connected to the first and second electrodes.
    Type: Application
    Filed: May 24, 2013
    Publication date: November 27, 2014
    Applicant: The Johns Hopkins University
    Inventors: Su Chih Chi, Robert Cammarata, Stephen L. Farias, Donglei Fan, Danru Qu, Chia-Ling Chien
  • Publication number: 20140329699
    Abstract: The present disclosure relates to systems and methods for high efficiency electronic sequencing of nucleic acids and molecular detection. In an example embodiment of the instant disclosure, the NanoNeedle may be utilized to detect a change in impedance resulting from the modulation of the counter ion concentration or Debye length associated with a biomolecule of interest, such as DNA or protein, for an application of interest, such as DNA sequencing, DNA hybridization, or protein detection.
    Type: Application
    Filed: December 3, 2012
    Publication date: November 6, 2014
    Inventor: Hesaam Esfandyarpour
  • Publication number: 20140318965
    Abstract: Provided herein is technology relating to depositing and/or placing a macromolecule at a desired site for an assay and particularly, but not exclusively, to methods and systems for placing or guiding a macromolecule such as a protein, a nucleic acid, or a protein:nucleic acid complex to an assay site, such as near a nanopore, a nanowell, or a zero mode waveguide.
    Type: Application
    Filed: December 21, 2012
    Publication date: October 30, 2014
    Inventor: Mark A. Hayden
  • Patent number: 8871063
    Abstract: A process for selective removal of a gaseous product (P) from a gaseous system comprising said product and other components (R1, R2), wherein the gaseous system is admitted to a first environment, which is separated from a second environment by a boundary wall, and a permeation membrane (3, 300) forms at least part of said boundary wall; a spatially non-uniform electric field (4) is generated between a first electrode or first plurality of electrodes (1, 301) located in the first environment and a second electrode or second plurality of electrodes (2, 302) located in the second environment, so that field lines of said non-uniform electric field cross said membrane, and a dielectrophoretic force generated on particles of said gaseous component (P) is at least part of a driving force of the permeation through said membrane, an amount of said product (P) being selectively removed from the first environment and collected in the second environment.
    Type: Grant
    Filed: June 10, 2011
    Date of Patent: October 28, 2014
    Assignee: Casale SA
    Inventor: Sergio Panza
  • Patent number: 8864972
    Abstract: Provided is a dielectrophoresis apparatus with which it is possible to handle (move, stop, separate and sort, etc.) a dielectric particle utilizing dielectrophoresis and to measure dielectrophoretic force. The interior of a dielectrophoresis device that includes a case having a flat top or bottom surface is filled with a dielectric solution S and accepts introduction of a small target body (particle) P comprising a dielectric. A non-uniform alternating electric field is formed within the case. By tilting the case (through an angle ?pitch or other direction), rotating the case in an inclined plane (through a rotational angle ?yaw) or adjusting the voltage and frequency of the alternating electric field, imbalance or balance is produced between a dielectrophoretic force FDEP that acts upon the small body and a force FG sin ?pitch ascribable to gravity and buoyancy, thereby enabling the small body to be moved and stopped.
    Type: Grant
    Filed: September 26, 2008
    Date of Patent: October 21, 2014
    Assignee: Kyushu Institute of Technology
    Inventors: Takeshi Yamakawa, Hiroko Imasato
  • Patent number: 8864973
    Abstract: A device for dielectrophoretic manipulation of suspended particulate matter comprises a plurality of interleaved layers of electrically conductive and non-conductive material wherein at least one channel is defined through a plurality of the interleaved layers of electrically conductive material.
    Type: Grant
    Filed: February 27, 2004
    Date of Patent: October 21, 2014
    Assignee: University of Surrey
    Inventors: Michael P. Hughes, Kai F. Hoettges, Stephen Ogin, Reg Wattingham
  • Publication number: 20140262787
    Abstract: The invention provides devices, systems, and methods for extracting target objects from a sample. In the method, a stream of sample containing a plurality of target and non-target objects is directed by first and second streams of buffer through a sample inlet channel into a fluid junction and through the fluid junction into a sample waste channel. In response to detecting a target object within the stream of sample, an actuator is energized to close a normally open valve, resulting in a transient burst of cross-flow into the fluid junction that briefly diverts the flow of sample within the fluid junction and results in an aliquot of sample being directed into an aliquot delivery channel. The combination of the valve and the actuator acts as a self-limiting pulse generator.
    Type: Application
    Filed: March 15, 2013
    Publication date: September 18, 2014
    Applicant: CALIPER LIFE SCIENCES, INC.
    Inventors: Joshua I. MOLHO, Daniel G. STEARNS, I-Jane CHEN, Danh TRAN, Bradley W. RICE, Tobias Daniel WHEELER, Alexander V. DUKHOVNY
  • Patent number: 8834810
    Abstract: A method and apparatus for microfluidic processing by programmably manipulating a packet. A material is introduced onto a reaction surface and compartmentalized to form a packet. A position of the packet is sensed with a position sensor. A programmable manipulation force is applied to the packet at the position. The programmable manipulation force is adjustable according to packet position by a controller. The packet is programmably moved according to the programmable manipulation force along arbitrarily chosen paths.
    Type: Grant
    Filed: July 10, 2012
    Date of Patent: September 16, 2014
    Assignee: Board of Regents, The University of Texas System
    Inventors: Frederick F. Becker, Peter Gascoyne, Xiaobo Wang, Jody Valentine Vykoukal, Giovanni De Gasperis
  • Patent number: 8834698
    Abstract: A method for sorting cells in a biological sample comprising a first type of cells and a second type of cells may comprise introducing the biological sample into a chamber comprising a first surface and a second surface, wherein the first surface is associated with a transparent electrode and the second surface is associated with a photoconductive portion of an electrode. The method may further comprise moving incident light and the photoconductive portion relative to one another so as to illuminate regions of the photoconductive portion and modulate an electric field in the chamber in proximity to the illuminated regions. The method may further comprise separating the first type of cells from the second type of cells in the chamber via dielectrophoretic movement of the first type of cells and the second type of cells caused by the modulated electric field, wherein a dielectrophoretic characteristic of at least one of the first type of cells and the second type of cells has been modified.
    Type: Grant
    Filed: February 8, 2010
    Date of Patent: September 16, 2014
    Assignee: Life Technologies Corporation
    Inventors: Aldrich Lau, Joon Yang, Huan Phan, Steven Sherwood, Hans Fuernkranz
  • Publication number: 20140251813
    Abstract: An analyte selection device can include: a body defining a fluid channel having a channel inlet and channel outlet; a bipolar electrode (BPE) between the inlet and outlet; one of an anode or cathode electrically coupled with the BPE on a channel inlet side of the BPE and the other of the anode or cathode electrically coupled with the BPE on a channel outlet side of the BPE; and an electronic system operably coupled with the anode and cathode so as to polarize the BPE. The fluid channel can have any shape or dimension. The channel inlet and channel outlet can be longitudinal or lateral with respect to the longitudinal axis of the channel. The BPE can be any metallic member, such as a flat plate on a wall or mesh as a barrier BPE. The anode and cathode can be located at a position that polarizes the BPE.
    Type: Application
    Filed: March 10, 2014
    Publication date: September 11, 2014
    Applicant: CFD Research Corporation
    Inventors: Yi Wang, Hongjun Song, Kapil Pant
  • Patent number: 8828212
    Abstract: Disclosed herein is a dielectric cytometric apparatus capable of analyzing cells and sorting the cells without adopting an optical analysis method and provides a dielectric cytometric cell sorting method for the apparatus. A stenosis channel allowing a single cell to flow is created on a cell injection section of a micro flow channel device used as a portion of the dielectric cytometric apparatus. A pair of measurement electrodes is created on the stenosis channel and an analyzer connected to the measurement electrodes measures the complex dielectric constant of for every cell passing through the stenosis channel. A electric-field application section provided on the downstream side of the stenosis channel applies an electric field for changing the flow of the cells inside a flow channel on the basis of information on the measured complex dielectric constants so that the cells can be sorted by making use of branch channels.
    Type: Grant
    Filed: October 21, 2011
    Date of Patent: September 9, 2014
    Assignee: Sony Corporation
    Inventors: Shinji Omori, Kazumasa Sato, Yoichi Katsumoto, Tomoyuki Umetsu, Yoshihito Hayashi
  • Publication number: 20140246322
    Abstract: Provided is a flow channel device including: a flow channel through which a fluid including particles flow; a plurality of branch channels branching from the flow channel; a measurement portion that is provided at a predetermined position in the flow channel and configured to measure electrical characteristics when the particles pass the predetermined position; an operation portion that is provided on a downstream side of the measurement portion and on an upstream side of the plurality of branch channels and configured to apply a dielectrophoretic force to the particles by forming an electric field; and an electrical first guard portion provided between the measurement portion and the operation portion.
    Type: Application
    Filed: February 25, 2014
    Publication date: September 4, 2014
    Applicant: SONY CORPORATION
    Inventors: Yoichi Katsumoto, Kazumasa Sato, Keiwa Sakai
  • Publication number: 20140246319
    Abstract: Droplet actuators and techniques for droplet-based assays are provided. A method of conducting an assay comprises: incubating a droplet in oil, the droplet comprising an umbelliferone substrate, a sample potentially comprising an enzyme which cleaves the umbelliferone substrate, and a zwitterionic surfactant; and detecting a signal emitted from the droplet.
    Type: Application
    Filed: July 11, 2012
    Publication date: September 4, 2014
    Applicant: ADVANCED LIQUID LOGIC, INC.
    Inventor: Theodore Winger
  • Patent number: 8821703
    Abstract: The invention relates to a method for identifying the source of shed bioparticles and an apparatus that implements the method. The method involves collecting a sample of bioparticles from the environment, selecting from that sample the bioparticles most effective in identifying their source, and gathering data from those bioparticles to form bioparticle signatures. The bioparticle signatures are then processed into a multi-dimensional vector which is then compared to the multi-dimensional vector derived from a standard using a pattern recognition strategy that identifies the source. The apparatus has a particle collection device to collect the sample, a transfer device that selects information-rich bioparticles and a detector that restricts the movement of the information-rich bioparticles. The restricted movement is then translated into a bioparticle signature.
    Type: Grant
    Filed: July 7, 2008
    Date of Patent: September 2, 2014
    Inventors: Mark A. Hayes, Thomas J. Taylor, Karl Booksh, Neal Woodbury, Pierre Herckes
  • Patent number: 8815070
    Abstract: Disclosed herein is a device A device of the microelectrode array architecture, comprising: (a) a bottom plate comprising an array of multiple microelectrodes disposed on a top surface of a substrate covered by a dielectric layer; wherein each of the microelectrode is coupled to at least one grounding elements of a grounding mechanism, wherein a hydrophobic layer is disposed on the top of the dielectric layer and the grounding elements to make hydrophobic surfaces with the droplets; (b) a field programmability mechanism for programming a group of configured-electrodes to generate microfluidic components and layouts with selected shapes and sizes; and, (c) a system management unit, comprising: (i) a droplet manipulation unit; and (ii) a system control unit.
    Type: Grant
    Filed: February 17, 2011
    Date of Patent: August 26, 2014
    Assignee: Sparkle Power, Inc.
    Inventors: Gary Chorng-Jyh Wang, Ching Yen Ho, Wen Jang Hwang, Wilson Wen-Fu Wang