Abstract: A mixing machine 1 comprising a mixing head 3 having element(s) for connecting to a receptacle to form a mixing container. The mixing head 3 is pivotably supported in a frame 2 such that the mixing container can pivot for the mixing process. The mixing head 3 has at least one rotationally driven mixing tool whose drive shaft 23 extends through the wall of the mixing head 3. The mixing head 3 has a double wall with a perforated inner wall 8. The perforations form a fluid path from the chamber surrounded by the inner wall 8 to the intermediate wall chamber 10. The intermediate wall chamber 10 is connected to a pressure adjustment device for adjusting the pressure. A flexible liner 15 is arranged in the mixing head 3 on its inner surface, which liner 15 can be fixed to the inner wall 8 of the mixing head by applying a vacuum.

Abstract: A chromatography column (2) containing a bed of packed particles (22, 24, 26, 28, 30), wherein the packed particles comprise fused core particles and the particle diameters of the packed particles vary along the column. Preferably, the particles (2, 24, 26, 28, 30) are arranged according to their average particle diameter, in order of increasing average particle diameter from the inlet end (4) to the outlet end (6). The bed may comprise a plurality of bed sections and each bed section has an average particle diameter calculated from the particles in that section and there are at least two different average particle diameter bed sections, wherein the particles of each bed are separated from particles of an adjacent bed by a partition that is liquid permeable to allow through a flow of mobile phase. A high column efficiency can be provided with lower pressure drop per unit length of the column.

Abstract: A measurement device includes mechanical support elements (101-104) for supporting a sample well, other mechanical support elements (105-109) for supporting a measurement head (112) suitable for optical measurements, and a control system (111) configured to control the measurement head to carry out at least two optical measurements from at least two different measurement locations inside the sample well, where each measurement location is a center point of a capture range from which radiation is captured in the respective optical measurement. The final measurement result is formed from the results of the at least two optical measurements in accordance with a pre-determined rule. The use of the at least two optical measurements from different measurement locations reduces measurement variation in situations where the sample well (153) contains a piece (158) of sample carrier.

Abstract: Fabricating a capacitor includes performing an oxide formation operation on a sheet of material. The oxide formation operation forms an anode metal oxide on an anode metal. A thermal compression is performed on the sheet of material after the oxide formation operation is performed. The thermal compression applies thermal energy to the sheet of material while applying pressure to the sheet of material. After the thermal compression, the capacitor is assembled such that at least one electrode in the capacitor includes at least a portion of the sheet of material.

Abstract: Polydisperse and charged polysaccharides are fractionated into low polydispersity fractions (preferably having pd<1.1), each containing species within a narrow range of molecular weights. An aqueous solution of the polydisperse polysaccharides is contacted with an ion exchange resin in a column and the polysaccharides are subjected to selective elution by aqueous elution buffer. The selective elution consists of at least 3 sequential elution buffers having different and constant ionic strength and/or pH and in which the subsequent buffers have ionic strength and/or pH than those of the preceding step. The new preparations are particularly suitable for the production of PSA-derivatized therapeutic agents intended for use in humans and animals.

Abstract: In a preparative separation-purification system for passing a solution containing a target component through a trap column to capture the target component in the column, and for subsequently passing an eluting solvent through the column to elute the captured component and collect it in a container, a dilution passage is merged with a collection passage for sending an eluate from the outlet end of the trap column to the collection container, and a diluting liquid is intermittently introduced through the dilution passage into the collection passage. The diluting liquid lowers the concentration of the target component in the eluate and impedes the deposition of the target component. Thus, clogging of the passage due to the deposition of the target component eluted from the trap column is effectively prevented.

Abstract: Polydisperse and charged polysaccharides are fractionated into low polydispersity fractions (preferably having pd<1.1), each containing species within a narrow range of molecular weights. An aqueous solution of the polydisperse polysaccharides is contacted with an ion exchange resin in a column and the polysaccharides are subjected to selective elution by aqueous elution buffer. The selective elution consists of at least 3 sequential elution buffers having different and constant ionic strength and/or pH and in which the subsequent buffers have ionic strength and/or pH than those of the preceding step. The new preparations are particularly suitable for the production of PSA-derivatised therapeutic agents intended for use in humans and animals.

Abstract: Processes for separating a di-carboxylic acid or salt thereof from a mixture containing the di-carboxylic acid or salt thereof and one or more other components are provided. Also separation media useful for these separation processes is provided. In particular, processes for preparing an aldaric acid are described, such as glucaric acid from glucose, which includes separating the aldaric acid from the reaction product. Also, various glucaric acid products are described.

Abstract: A supercritical fluid chromatography system is provided with an injection valve subsystem for introducing a sample into a flow of mobile phase fluid. The injection valve subsystem includes an auxiliary valve and an inject valve. The operations of the auxiliary and inject valves are coordinated in such a manner as to reduce sample carry-over and system pressure perturbations occurring during sample injection.

Abstract: A supercritical fluid chromatograph includes a supercritical flow path, a mobile phase supply section for supplying a mobile phase containing liquid carbon dioxide, a sample introduction section, a sample separation section, a detector, and a pressure control valve. A valve post-stage flow path is connected to a fluid outlet of the pressure control valve, and the inside of the valve post-stage flow path is maintained by pressure maintaining means at a pressure by which a mobile phase from the fluid outlet of the pressure control valve is not vaporized.

Abstract: The present invention aims at providing an analyzing apparatus control system which is capable of appropriately setting the conditions of measurement in an analyzing apparatus which is connected to a chromatograph, in which one more measurement events are performed based on a reference chromatogram. This system includes a measurement time range setting section for setting, for each of all peaks or previously selected peak or peaks included in a previously provided reference chromatogram which corresponds to the sample to be examined, the width of a peak as the measurement time range of the peak when the peak does not overlap another peak, or, when the peak overlaps another peak, the overall width of the peak and the overlapping peak as the measurement time range of the peak.

Abstract: The present invention relates to a method for purifying a protein by separating the protein from impurities in a non-adsorption mode using an activated carbon. In particular, the present invention relates to a method for purifying an antibody using the activated carbon instead of protein A affinity chromatography.

Abstract: An ion electrospray device with a porous reservoir and at least one porous emitter includes a porous compliant interface sandwiched between the porous reservoir and the porous emitter for transferring fluid from the porous reservoir to the porous emitter. The interface has a characteristic capillary pressure stronger than the characteristic capillary pressure of the porous reservoir to fill the porous emitter with fluid via a fluid injection section of the interface and before the porous reservoir is then partially filled with fluid. Emitter leakage and propellant bridging problems are addressed.

Abstract: The embodiments disclose a method of surface tension control to reduce trapped gas bubbles in an imprint including modifying chemistry aspects of interfacial surfaces of an imprint template and a substrate to modify surface tensions, differentiating the interfacial surface tensions to control interfacial flow rates of a pre-cured liquid resist and controlling pre-cured liquid resist interfacial flow rates to reduce trapping gas and prevent trapped gas bubble defects in cured imprinted resist.

Abstract: A serviceable system for handling fluids in microgravity includes supply reservoirs to hold a supply fluid for a process, a fluid processing unit to perform the process and a collection reservoir to collect a fluid from the fluid processing unit. Each supply reservoirs is provided with a supply valve assembly of valves and conduits, and at least one external conduit that is connectable to an external fluid source or to an external suction source. The supply valve assembly is configured to enable individually: flow of the supply fluid from the supply reservoir to the fluid processing unit, flow of a fluid into the supply reservoir from the external conduit; withdrawal of fluid from supply reservoir to the external conduit, and flow of fluid from the external conduit to the fluid processing unit.

Abstract: The present invention relates to a skin care composition comprising an extract of Bursera simaruba seeds and a cosmetically acceptable topical carrier. Such composition is useful for improving skin barrier function and moisturization as well as improving signs of skin aging.

Abstract: Apparatus and methods for creating a pulsed ion beam. The pulsed ion beam can be used for performing mass spectrometry. A pulsed solenoid valve can provide a pulsed ion beam from an electrospray in a pre-vacuum chamber. The pulsed ion beam can enter a high vacuum region and a mass analyzer for mass spectrometry.

Abstract: Methods and apparatus consistent with the invention provide the ability to organize, index, search, and present time series data based on searches. Time series data are sequences of time stamped records occurring in one or more usually continuous streams, representing some type of activity. In one embodiment, time series data is organized into discrete events with normalized time stamps and the events are indexed by time and keyword. A search is received and relevant event information is retrieved based in whole or in part on the time indexing mechanism, keyword indexing mechanism, or statistical indices calculated at the time of the search.

Abstract: The invention generally relates to ion-exchange chromatography. More particularly, the invention relates to compositions and methods that can substantially improve analytical sensitivity and/or selectivity of ion-exchange chromatography as well its efficiency and quality as a purification or preparation tool.

Abstract: Embodiments of the present disclosure present novel systems, devices and methods for an automated biological sample analysis using mass-spectrometry. The time from sample introduction to the reporting of data, in some embodiments, takes a relatively short amount of time (e.g., several minutes). In some embodiments, a biological sample to be analyzed is a blood sample. For many applications, only a single drop of blood may be sufficient. Through the use of a mixture of standards with unique molecular mass, a quantitative analysis of the target analyte can be performed in a single MS run (for example), eliminating the need to create and analyze standard curves. One advantage of such embodiments may be that the system, devices, and methods can eliminate the need for batch creation since the requirement to amortize the time and effort of creating and analyzing standard curves can be eliminated.

Abstract: HPLC methods for detecting, identifying and quantifying munitions compounds or munitions materials are disclosed. Insensitive munitions explosives (IMX) can be detected along with conventional munitions compounds such as 2,4,6-trinitrotolene (TNT) in a single column analysis. The methods are also useful to provide analytical evaluation of soil samples, aqueous samples such as ground water samples and tissue samples containing insensitive munitions explosives (IMX).

Abstract: The invention relates to containers or bags for chromatographic media and methods of packing chromatography columns using such containers. The bags may be used for storing and/or transporting chromatographic media and can be inserted directly into the chamber of a chromatography column in readiness for use.

Abstract: The present disclosure relates to compositions comprising gymnemic acid, together with a form of zinc to block the unpleasant bitter taste of gymnemic acid as well as to extend the sweet taste blocking properties of gymnemic acid, resulting in palatable compositions for delivery to the oral cavity to block sweet taste receptors located therein. The present disclosure also relates to methods of reducing sugar consumption and reducing calorie intake via administration of such compositions to a subject.

Abstract: A method for characterizing the saturates portion of a petroleum or hydrocarbon sample that includes compounds with boiling points of 1000° F. (538° C.) or higher includes use of laser desorption ionization (LDI) to desorb and vaporize petroleum saturates into the gas phase. After ionization, the saturate compounds cations can be detected using mass spectrometry. The mass spectrum generated from the ionized saturated compounds is then characterized by assigning molecular formulas to any “detected” masses that exhibit a peak with an intensity greater than a defined signal to noise threshold. After making the molecular assignments, the abundance of each assigned molecule can be determined based on the signal magnitude of the peaks in the mass spectrum. The assigned molecules and the corresponding abundances can then be grouped based on a variety of factors.

Abstract: Porous block nano-fiber composite (110), a filtration system (10) and methods of using the same are disclosed. An exemplary porous block nano-fiber composite (110) includes a porous block (100) having one or more pores (200). The porous block nano-fiber composite (110) also includes a plurality of inorganic nano-fibers (211) formed within at least one of the pores (200).

Abstract: The invention relates to a batch chromatography process the purification of (per)fluoropolyether (PFPE) carboxylates, in particular to a process for increasing the average functionality of PFPE carboxylates mixtures.

Abstract: A protective structure and an electronic device using the same are disclosed. The electronic device includes a first housing member, a second housing member, a blocking unit, and an operating module. The second housing member faces the first housing member, wherein a slit is located at the periphery of an enclosure disposed between the first and second housing members. The blocking unit, having a shape compatible with the shape of the slit, is disposed therein and includes a sealing member and a first foamable material. The sealing member is compressed by the first and second housing members, and the first foamable material is formed in the slit along an extending direction of the sealing member.

Abstract: A multifunctional device for chromatographic separation, in particular for affinity chromatographic separation of a substance mixture, provides for a uniform fluid distribution on a separation medium and a uniform flow through a stationary phase. The fluid to be separated is passed through a radial inlet line in an upper partial region of the device, to the center of the upper partial region. The radial inlet line is enlarged in the form of a cupola at the center of the upper partial region.

Abstract: The present invention provides a cleaning method by which the performance of a microchip can be recovered and a cleaning liquid. A method for cleaning a microfluidic device comprising: cleaning a channel that is formed in the microfluidic device, has a surface having a polymer coating, and has been brought into contact with a sample containing nucleic acid and/or protein, by bringing the channel into contact with a cleaning liquid comprised only of an organic solvent having solubility in at least the same volume of water at 25° C., or a cleaning liquid containing 50 vol % or more of the organic solvent in a buffer solution. The method wherein the buffer solution has a pH of 8 to 10. The method wherein the buffer solution further contains 3 to 8M of a protein denaturant. The method wherein the buffer solution has a pH of 2 to 4.

Abstract: There is a tendency of the intensity and the shape of a spectrum to be measured transitioning with the passage of measured time, depending on the volatility and the reactivity of a component. A mass spectrometric system includes: a mass spectrometric unit that measures a specimen and outputs a mass spectrum; and an estimator that has an estimation rule on content information, the estimation rule being assigned to each component and each measurement time. The estimator estimates, based on a mass spectrum output from the mass spectrometric unit, content information on each component of a plurality of components that may be contained in the specimen in accordance with the estimation rule.

Abstract: The embodiments disclose a method of surface tension control to reduce trapped gas bubbles in an imprint including modifying chemistry aspects of interfacial surfaces of an imprint template and a substrate to modify surface tensions, differentiating the interfacial surface tensions to control interfacial flow rates of a pre-cured liquid resist and controlling pre-cured liquid resist interfacial flow rates to reduce trapping gas and prevent trapped gas bubble defects in cured imprinted resist.

Abstract: A method and device for enhanced capture of target analytes is disclosed. This invention relates to collection of chemicals for separations and analysis. More specifically, this invention relates to a solid phase microextraction (SPME) device having better capability for chemical collection and analysis. This includes better physical stability, capacity for chemical collection, flexible surface chemistry and high affinity for target analyte.

Abstract: Collection containers (17) are heated by head conduction from a container rack (18) with a heater (19) as the heat source. When an eluting solvent is supplied into a trap column (7) by a pump (5), an eluate containing a target compound exiting from the column (7) flows through a preparative separation passage (13) and drips from a solution nozzle (13a), and this solution is separated into fine droplets by a gas stream blowing from a gas ejection nozzle (15a). When a droplet touches an inner wall of the container (17), the volatile solvent immediately vaporizes, leaving the target compound precipitated in solid forms on the inner wall. Thus, the process of vaporizing and drying the eluate to collect the target compound is completed within a short period of time. This process can be performed online and hence is suitable for laborsaving.

Abstract: At least one system for eluting a radioactive material and a method of eluting a radioactive material is provided. The system for eluting a radioactive material may include an elution column configured to enclose an radioactive material, a first sealing member sealing a first end of the elution column, a second sealing member sealing a second end of the elution column, an elution supply source connected to the first end of the elution column via a first needle, a collection system connected to the second end of the elution column via a second needle, and a filter in the elution column, the filter being configured to support the radioactive material and prevent the radioactive material from contacting the second needle.

Abstract: The object of the present invention is to provide a sliding member, a prosthesis and a method of producing the sliding member, which can suppress production of abrasive wear debris by suppressing friction of the sliding section, and also can maintain satisfactory mechanical characteristics in vivo. A sliding member comprising: a substrate 1 capable of forming hydroxyl groups; and a biocompatible material layer 4 laminated on appropriate sections of the substrate 1, wherein hydroxyl groups are formed on at least a required section of a surface of the substrate 1 by surface treating to form a surface-treated layer 2, while the biocompatible material layer 4 is formed from a polymer containing phosphorylcholine groups, and wherein the substrate 1 and the biocompatible material layer 4 are joined via a binder layer 3 formed from silica being covalently bonded with the hydroxyl groups and the biocompatible material, respectively.

Abstract: This invention provides for the fully automated, hands free, packing of chromatography columns by means of delivering a pre-calculated volume of slurry and using two different packing modalities to stop the packing when either 1) this volume has been delivered in the column, or 2) when the adapter is moved to reach a bed height corresponding to the pre-calculated volume. Thus, a chromatography column can be packed in a fully automated fashion and such a column is 1) stable and 2) has the desired performance characteristic.

Abstract: A type of liquid chromatographic stationary phase and preparation method thereof, the bonding terminal of the chromatographic stationary phase is zwitterionic functional group. The preparation method includes the following steps, alkenyl or alkynyl silane is bonded onto the surface of silica based on the horizontal polymerization approach to obtain alkenyl- or alkynyl-modified silica. Then the thiol click reaction with zwitterionic compound containing thiol group is performed to obtain the zwitterionic hydrophilic interaction chromatographic stationary phase. The present stationary phase possesses both zwitterionic characteristics and excellent hydrophilicity. It can be widely applied in the separation of variety of samples.

Abstract: A chromatography column and method of maintenance is described which does not require the use of a hoist or crane for disassembly. The method provides improved operator safety by reducing the need for the operator to work below a suspended or supported load within the column. Furthermore, the removal or replacement of column components is facilitated by providing access to the interior of the column and by the provision of a handling device.

Abstract: Novel particles and materials for chromatographic separations, processes for preparation and separations devices containing the chromatographic particles and materials are provided by the instant invention. In particular, the invention provides a porous inorganic/organic hybrid particle, wherein the inorganic portion of the hybrid particle is present in an amount ranging from about 0 molar % to not more than about 49 molar %, wherein the pores of the particle are substantially disordered. The invention also provides a porous inorganic/organic hybrid particle, wherein the inorganic portion of the hybrid particle is present in an amount ranging from about 25 molar % to not more than about 50 molar %, wherein the pores of the particle are substantially disordered and wherein the particle has a chromatographically enhancing pore geometry (CEPG). Methods for producing the hybrid particles, separations devices comprising the hybrid particles and kits are also provided.

Abstract: A column packing system comprises a control unit provided with software for monitoring the column interior pressure and/or flow through the column for use in calculating the breakpoint where a movable adapter comes into contact with a consolidated bed of bed media. The calculated breakpoint is used by the control unit to determine how much further the movable adapter has to move into the column in order to achieve a desired amount of bed compression.

Abstract: Methods and systems for analyzing ratios of analytes within a flowing sample are provided. The flowing sample can be processed in real-time to determine a time interval over which a predetermined amount of a group of analytes passes by a fixed point in a flow cell. The predetermined amount can be routed to a sample container for future processing. The sample can comprise diluted blood and the analytes can comprise a component of hemoglobin, such as A1c, and the total amount of hemoglobin, of which the predetermined amount is metered.

Abstract: Methods and kits for regenerating antibody binding resins are provided. In some embodiments the methods include a first step of washing an antibody binding resin with a reducing solution, followed by a second step of washing the antibody binding resin with a chaotropic solution.

Abstract: Methods using homogeneous precipitation of a metal on a surface of a particle to prepare silica particles having the metal adsorbed thereon are disclosed herein. In certain embodiments, the silica particles having the metal adsorbed thereon can be used to prepare carbon coated silica particles. The carbon coated silica particles can be useful in a wide variety of applications including, for example, for use as sorbents in chromatography.

Abstract: Provided are a filler for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler in which a protein represented by the following formula (1) is immobilized on a carrier. R—R2??(1) wherein R represents an amino acid sequence consisting of 4 to 300 amino acid residues containing a region consisting of 4 to 20 contiguous histidine residues; and R2 represents an amino acid sequence capable of binding to immunoglobulin, the amino acid sequence consisting of 50 to 500 amino acid residues containing Z domain of Protein A or a fragment thereof, or a variant thereof, provided that the R binds to C-terminus or N-terminus of the R2.

Abstract: Herein is reported a method for determining whether a re-usable chromatography column packing, which is used at least for the second time in a purification step of a purification of a polypeptide, has reduced separation efficacy in said purification step of said purification of said polypeptide, comprising the following steps: a) identifying and determining the experimental data of an inert change of at least one physicochemical parameter of a mobile phase passing through said re-usable chromatography column packing, b) determining the parameters of a function of formula I by fitting the experimental data of the inert change of the physicochemical parameter of the at least second use, c) determining the difference between the experimental data of the inert change of the physicochemical parameter of the at least second use and the function of formula I with the parameters determined in step b), d) calculating the difference between the maximum value and the minimum value of the difference determined in step c)

Abstract: The present invention provides a chromatographic stationary phase material various different types of chromatography. One example chromatographic stationary phase is represented by Formula 1 [X](W)a(Q)b(T)c (Formula 1). X can be a high purity chromatographic core composition. W can be absent and/or can include hydrogen and/or can include hydroxyl on the surface of X. Q can be bound directly to X and can include a first hydrophilic, polar, ionizable, and/or charged functional group that chromatographically interacts with the analyte. T can be bound directly to X and can include a second hydrophilic, polar, ionizable, and/or charged functional group that chromatographically interacts with the analyte. Additionally, Q and T can essentially eliminate chromatographic interaction between the analyte, and X and W, thereby minimizing retention variation over time (drift or change) under chromatographic conditions utilizing low water concentrations.

Abstract: Guanidinyl ligand-functionalized polymers, methods of making the same, and substrates bearing a grafted coating of the ligand-functional polymers are described. The grafted polymer has the requisite affinity for binding neutral or negatively charged biomaterials, such as cells, cell debris, bacteria, spores, viruses, nucleic acids, endotoxins and proteins, at pH's near or below the pI's of the biomaterials.

Abstract: A chromatography column is prepared with a stationary phase comprising a deuterated poly(ethyleneglycol), or other deuterated polymer. Formation of the stationary phase can be performed using exactly the same methodology as used when forming a stationary phase with the equivalent non-deuterated polymer. The deuterated poly(ethyleneglycol), or other deuterated polymer, preferably has increased thermal stability as compared to non-deuterated poly(ethyleneglycol), or equivalent non-deuterated polymer. This reduces bleeding of the stationary phase during gas chromatography and allows the use of greater operating temperatures.

Abstract: A flash chromatography apparatus which provides an improved apparatus for large and medium commercial scale flash chromatography columns. More particularly, the invention relates to a flash chromatography column and cartridge system for positioning and ease of loading and unloading of stationary phase agent in the flash chromatography column. The flash chromatography column further provides flow distributors to support the stationary phase and permit plug flow through the column. The apparatus of the present is modular and can be disposed in any configuration to reduce maintenance cost and downtime in a commercial installation. Flash chromatography is widely used for purification of low molecular weight organic compounds and products of organic synthetic reactions.

Abstract: In one aspect, the present invention provides a chromatographic stationary phase material for various different modes of chromatography represented by Formula 1: [X](W)a(Q)b(T)c (Formula 1). X can be a high purity chromatographic core composition having a surface comprising a silica core material, metal oxide core material, an inorganic-organic hybrid material or a group of block copolymers thereof. W can be absent and/or can include hydrogen and/or can include a hydroxyl on the surface of X. Q can be a functional group that minimizes retention variation over time (drift) under chromatographic conditions utilizing low water concentrations. T can include one or more hydrophilic, polar, ionizable, and/or charged functional groups that chromatographically interact with the analyte. Additionally, b and c can be positive numbers, with the ratio 0.05?(b/c)?100, and a?0.