And Means Segmenting Fluid Material Patents (Class 422/82)
  • Patent number: 8389272
    Abstract: A method and apparatus for delivering one or more fluids. Fluids may be delivered sequentially from a common vessel to a chemical, biological or biochemical process.
    Type: Grant
    Filed: September 6, 2011
    Date of Patent: March 5, 2013
    Assignee: President and Fellows of Harvard College
    Inventors: Vincent Linder, Samuel K. Sia, George M. Whitesides
  • Patent number: 8389294
    Abstract: A microfluidic device and method is provided for coupling discrete channels and for co-culture. The microfluidic device includes first and second bodies. Each body has a bottom surface and defines a channel. The channel in each body includes an inlet and an outlet communicating with the bottom surface. A first fluid, such as a first cell suspension, is provided within the channel of the first body and a second fluid, such a second cell suspension, is provided within the channel of the second body. The first and second bodies are movable between a first position wherein the outlet of the channel of the first body is spaced from the inlet of the channel of the second body and a second position wherein the fluid at the outlet of the channel of the first body communicates with the fluid at the inlet of the channel of the second body.
    Type: Grant
    Filed: June 9, 2008
    Date of Patent: March 5, 2013
    Assignee: Wisconsin Alumni Research Foundation
    Inventors: David J. Beebe, Jay W. Warrick
  • Patent number: 8383061
    Abstract: Fluid-based no-moving part logic devices are constructed from complex sequences of micro- and nanofluidic channels, on-demand bubble/droplet modulators and generators for programming the devices, and micro- and nanofluidic droplet/bubble memory elements for storage and retrieval of biological or chemical elements. The input sequence of bubbles/droplets encodes information, with the output being another sequence of bubbles/droplets or on-chip chemical synthesis. For performing a set of reactions/tasks or process control, the modulators can be used to program the device by producing a precisely timed sequence of bubbles/droplets, resulting in a cascade of logic operations within the micro- or nanofluidic channel sequence, utilizing the generated droplets/bubbles as a control. The devices are based on the principle of minimum energy interfaces formed between the two fluid phases enclosed inside precise channel geometries.
    Type: Grant
    Filed: April 4, 2011
    Date of Patent: February 26, 2013
    Assignee: Massachusetts Institute of Technology
    Inventors: Manu Prakash, Neil Gershenfeld
  • Patent number: 8383060
    Abstract: An integrated electronic-micro fluidic device an integrated electronic-micro fluidic device, comprising a semiconductor substrate on a first support, an electronic circuit on a first semiconductor-substrate side of the semiconductor substrate, and a signal interface structure to an external device. A micro fluidic structure is formed in the semiconductor substrate, and is configured to confine a fluid and to allow a flow of the fluid to and from the microfluidic structure only on a second semiconductor-substrate side that is opposite to the first semiconductor-substrate side and faces away from the first support.
    Type: Grant
    Filed: March 7, 2007
    Date of Patent: February 26, 2013
    Assignee: Koninklijke Philips Electronics N.V.
    Inventors: Ronald Dekker, Remco Henricus Wilhelmus Pijnenburg, Nicolaas Johannes Anthonius Van Veen
  • Patent number: 8372357
    Abstract: A rotatable microfluidic device that comprises a hydrophilic microchannel structure in which there is a) an upstream microcavity I with a liquid outlet I, b) a microconduit I connected to liquid outlet I, and c) a capillary valve I associated with microconduit I. The inlet end of the microconduit is closer to the spin axis than the outlet end of the microconduit. The difference in radial distance between the inlet end and the outlet end of microconduit I is typically ?5%, such as ?10% or ?100% or ?500%, of the difference in radial distance between the uppermost part of the upstream microcavity and liquid outlet I.
    Type: Grant
    Filed: April 13, 2006
    Date of Patent: February 12, 2013
    Assignee: Gyros Patent AB
    Inventors: Per Andersson, Gunnar Ekstrand, Gérald Jesson
  • Patent number: 8303909
    Abstract: Embodiments of a microfluidic assembly comprise at least two adjacent microstructures and a plurality of interconnecting fluid conduits which connect an outlet port of one microstructure to an inlet port of an adjacent microstructure. Each microstructure comprises an inlet flow path and an outlet flow path not aligned along a common axis. Moreover, the microfluidic assembly defines a microfluidic assembly axis along which respective inlet ports of adjacent microstructures are oriented or alternatively along which respective outlet ports of adjacent microstructures are oriented, and each microstructure is oriented relative to the microfluidic assembly axis at a nonorthogonal angle.
    Type: Grant
    Filed: February 23, 2010
    Date of Patent: November 6, 2012
    Assignee: Corning Incorporated
    Inventor: Mark Stephen Friske
  • Patent number: 8298833
    Abstract: A bridge (30) comprises a first inlet port (31) at the end of a capillary, a narrower second inlet port (32) which is an end of a capillary, an outlet port (33) which is an end of a capillary, and a chamber (34) for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber (34). The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber (34). The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port (32) causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port (32), and the droplets combine at the liquid bridge (30).
    Type: Grant
    Filed: February 7, 2007
    Date of Patent: October 30, 2012
    Assignee: Stokes Bio Limited
    Inventors: Mark Davies, Tara Dalton, Kieran Curran
  • Patent number: 8298497
    Abstract: A delivery apparatus for selectively delivering one or more liquid reagents into a reaction or test chamber (2), especially of an assay apparatus, the apparatus comprising: one or more respective storage chambers (5,6) for containing the one or more liquid reagents and arranged generally above the reaction or test chamber (2); and a plunger element (4) arranged and operable for insertion into the mouth of a selected storage chamber so as to displace a selected reagent from therewithin into the reaction or test chamber (2) generally therebelow by gravitational liquid overflow from the mouth of the chamber. The apparatus may conveniently be provided as a discrete delivery unit, with the storage chambers (5,6) prefilled with the selected reagents.
    Type: Grant
    Filed: December 14, 2009
    Date of Patent: October 30, 2012
    Assignee: The Secretary of State for Defence
    Inventor: David James Squirrell
  • Patent number: 8287708
    Abstract: A microfluidic system for creating encapsulated droplets whose shells can be further removed comprises: two electrode plates and a spacing structure disposed between the two electrode plates. One of the electrode plates has three reservoir electrodes and a plurality of channel electrodes. The three electrodes are respectively used for accommodating a shell liquid, a core liquid, and a removing liquid which is able to remove the shell liquid. The channel electrodes are used for communicating droplets among the three reservoir electrodes. Via these arrangements, the microfluidic system can create a quantitative shell droplet and a quantitative core droplet, and then merge the shell and core droplets to form an encapsulated droplet. Moreover, the shell of the encapsulated droplet can be removed by mixing it with the removing liquid. This invention is further provided with a method for creating an encapsulated droplet with a removable shell.
    Type: Grant
    Filed: June 15, 2010
    Date of Patent: October 16, 2012
    Assignee: National Chiao Tung University
    Inventors: Shih-Kang Fan, Yao-Wen Hsu, Chiun-Hsun Chen
  • Patent number: 8277729
    Abstract: The present invention is to present a sample analyzer which is capable of respond immediately when a need to perform analysis of multiple items arises. The sample analyzer 1 includes a table 12 capable of holding a first rack 320 and a second rack 330; a reagent dispensing arm 120 which comprises a pipette part 121; a reagent dispensing driving section 120a for moving the reagent dispensing arm 120; a reagent barcode reader 350; and a control section 501 for controlling the reagent dispensing driving section 120a so as to move the pipette part 121 to a predetermined reagent aspirating position according to the identification information obtained by the reagent barcode reader 350.
    Type: Grant
    Filed: March 27, 2008
    Date of Patent: October 2, 2012
    Assignee: Sysmex Corporation
    Inventors: Naohiko Matsuo, Hiroyuki Fujino, Takamichi Naito, Nobuhiro Kitagawa
  • Patent number: 8277744
    Abstract: The invention provides a device, system, and method for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. The device includes first and second pinching channels, a separation channel extending between the first and second pinching channels, a collection leg that includes a collection well between first and second ends of the collection leg, and a waste leg, all of which are in fluid communication with a switching region. In the method, a sample material is separated into a plurality of separated components in the device and one or more of the separated components are isolated in the collection well. The separated components are constrained and elongated in the switching region by first and second buffer streams.
    Type: Grant
    Filed: May 14, 2012
    Date of Patent: October 2, 2012
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Josh Molho, Hui Xu, Jorge J. Zaninovich
  • Patent number: 8277731
    Abstract: To shorten the time required for an oscillation frequency to stabilize in a sensing device sensing a substance to be sensed by using the fact that a natural frequency of a piezoelectric resonator changes when the substance to be sensed is adsorbed by an adsorption layer formed on the piezoelectric resonator. A sensing device includes: an oscillator circuit 4 oscillating a quartz-crystal resonator 12; and an intense excitation circuit that is formed by the oscillator circuit 4 including a series circuit of a resistor 52 and a third transistor 53 of PNP type and that intensely excites the quartz-crystal resonator 12 connected to the oscillator circuit 4 for a period of time preset by a one-shot circuit 22 by supplying the quartz-crystal resonator 12 with high power equal to or more than twice regular power supplied at the time of the measurement of the substance to be sensed, to stabilize the oscillation of the quartz-crystal resonator 12.
    Type: Grant
    Filed: April 14, 2010
    Date of Patent: October 2, 2012
    Assignee: Nihon Dempa Kogyo Co., Ltd.
    Inventors: Tomoya Yorita, Junichiro Yamakawa, Shigenori Watanabe, Mitsuaki Koyama
  • Patent number: 8273312
    Abstract: A liquid testing assembly for testing a liquid, the assembly comprising a test vessel and a stopper adapted to fit into a free end of the vessel. The stopper substantially hermetically seals the test vessel from the ambient. Further, the assembly includes a support coated with one or more identifying materials for identifying one or more constituents of the liquid. The support is fixed in the stopper and/or the vessel and extends into its interior for a predetermined distance. The liquid testing assembly when assembled is pre-evacuated to a predetermined vacuum sufficient to draw a predetermined volume of liquid to be sampled into the test vessel. The predetermined volume is of such an amount that it wets the one or more identifying materials ensuring identification of one or more constituents present in the liquid. A kit employing the liquid testing assembly is also discussed.
    Type: Grant
    Filed: January 22, 2008
    Date of Patent: September 25, 2012
    Assignee: Association for Public Health
    Inventors: Gadi Porat, Joel Stern, Boaz Rimon, Yoram Cohen
  • Patent number: 8273308
    Abstract: This disclosure provides systems, methods, and devices for processing samples on a microfluidic device. One system includes a microfluidic device having an upstream channel, a DNA manipulation zone located downstream from the upstream channel and configured to perform PCR amplification of a sample, a first valve disposed upstream of the DNA manipulation zone, and a second valve disposed downstream of the DNA manipulation zone. The system also includes a controller programmed to close the first and second valves to prevent gas and liquid from flowing into or out of the DNA manipulation zone, and a computer-controlled heat source in thermal contact with the DNA manipulation zone.
    Type: Grant
    Filed: October 30, 2007
    Date of Patent: September 25, 2012
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Gene Parunak
  • Patent number: 8268633
    Abstract: A method for conducting a broad range of biochemical analyses or manipulations on a series of nano- to subnanoliter reaction volumes and an apparatus for carrying out the same are disclosed. The invention is implemented on a fluidic microchip to provide high serial throughput. In particular, the disclosed device is a microfabricated channel device that can manipulate nanoliter or subnanoliter reaction volumes in a controlled manner to produce results at rates of 1 to 10 Hz per channel. The reaction volumes are manipulated in serial fashion analogous to a digital shift register. The invention has application to such problems as screening molecular or cellular targets using single beads from split-synthesis combinatorial libraries, screening single cells for RNA or protein expression, genetic diagnostic screening at the single cell level, or performing single cell signal transduction studies.
    Type: Grant
    Filed: April 5, 2007
    Date of Patent: September 18, 2012
    Assignee: UT-Battelle, LLC
    Inventors: J. Michael Ramsey, Stephen C. Jacobson
  • Patent number: 8268571
    Abstract: The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.
    Type: Grant
    Filed: August 11, 2011
    Date of Patent: September 18, 2012
    Assignee: STC.UNM
    Inventors: Larry A Sklar, Bruce Edwards, Frederick Kuckuck
  • Patent number: 8256747
    Abstract: A system and method for closed-loop testing of a biological or chemical sensor is disclosed. The tester is fluidically coupled to the input port and exhaust port of a sensor. A mixture of a chemical or biological simulant and air is generated by the tester and is delivered to the sensor. The mixture of simulant and air exiting the sensor is delivered to a collection device.
    Type: Grant
    Filed: October 18, 2006
    Date of Patent: September 4, 2012
    Assignee: Lockheed Martin Corporation
    Inventor: Kevin J. Tupper
  • Patent number: 8252539
    Abstract: A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix.
    Type: Grant
    Filed: October 8, 2007
    Date of Patent: August 28, 2012
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Todd Thorsen
  • Patent number: 8202486
    Abstract: The invention provides a device, system, and method for isolating one or more sample components of a sample material following separation of the sample material into a plurality of sample components. The device includes first and second pinching channels, a separation channel extending between the first and second pinching channels, a collection leg that includes a collection well, and a waste leg, all of which are in fluid communication with a switching region. In the method, a sample material is separated into a plurality of separated components in the device. and one or more of the separated components are isolated in the collection well. The separated components are constrained and elongated in the switching region by first and second buffer streams.
    Type: Grant
    Filed: July 26, 2010
    Date of Patent: June 19, 2012
    Assignee: Caliper Life Sciences, Inc.
    Inventors: Josh Molho, Hui Xu, Jorge J. Zaninovich
  • Patent number: 8202733
    Abstract: A system and method for obtaining hydrodynamic focusing of a first fluid. The method includes pressurizing the first fluid and a second fluid at a pre-defined pressure from a pressure source. Further, the method includes controlling the first flow rate of the first fluid by passing it through a first flow circuit. Furthermore, the method includes controlling the second flow rate of the second fluid by passing it through a second flow circuit. Moreover, the method includes passing the first and the second fluid though a converging section and hydrodynamically focusing the first fluid.
    Type: Grant
    Filed: December 14, 2007
    Date of Patent: June 19, 2012
    Inventor: Shervin Javadi
  • Patent number: 8143073
    Abstract: An apparatus is disclosed for carrying out an analysis process, in which probe molecules which are immobilized on a substrate within an analysis area are brought into contact with an analyte solution, which contains target molecules as reaction partners, and reaction events are detected between target molecules and probe molecules with the aid of magnetic marker particles which are coupled to the target molecules or to the probe molecules. An inhomogeneous magnetic field is applied to the analysis area before and/or after detection. In the apparatus, magnetic-field devices are provided at least for the production of an inhomogeneous magnetic field acting in the analysis area, with these magnetic-field devices being part of sequence control for determination of the bonding forces of the reaction events.
    Type: Grant
    Filed: August 11, 2006
    Date of Patent: March 27, 2012
    Assignee: Siemens Aktiengesellschaft
    Inventor: Joachim Bangert
  • Patent number: 8143070
    Abstract: A use composition monitor determines the concentration of peracid and/or peroxide in a use composition using a kinetic assay procedure. A sample mixture containing a sample of the use composition, a diluent and at least one reagent is prepared and analyzed using, for example, an optical detector. A reduced-turbulence optical detector can be used to improve collected response data. A reduced-turbulence optical detector can include a cell body disposed about a length of transparent tubing. The cell body positions one or more emitter/receiver pairs about the transparent tubing. Thus, tube junctions are eliminated and sample flow within the tube is substantially turbulence free.
    Type: Grant
    Filed: February 12, 2009
    Date of Patent: March 27, 2012
    Assignee: Ecolab USA Inc.
    Inventors: Eugene Tokhtuev, Christopher Owen, Anna Pilipchenko, Paul Schilling, Daniel Kamben
  • Patent number: 8137623
    Abstract: The present invention provides an apparatus for analyzing easily not only the overall taste of a sample but also what kind of components, synergetic effects and diminishing effects contribute to the taste. A sample to be analyzed is injected into a mobile phase by a liquid sending pump, and the sample is sent to a taste detector by way of a blending means and valves. Then, detection signals are obtained by taste sensors on the taste detector. The sample sent through the taste detector is introduced into a column by way of a 6-port-2-position valve, temporally separated into components and eluted from the column. After each component is detected by a UV detector, the eluted liquid is sent to the taste detector once more, and detection signals which reflect the tastes of each sample component. When analyzing how the taste changes by an additive, send the additive to the blending means and blend it with the sample.
    Type: Grant
    Filed: June 6, 2007
    Date of Patent: March 20, 2012
    Assignees: Shimadzu Corporation, Takasago International Corporation
    Inventors: Yosuke Iwata, Tsuyoshi Kobayashi
  • Patent number: 8124413
    Abstract: The invention relates to continuous flow systems, in particular thermocyclers for the automated and continuous cycling of fluid between a plurality of temperature zones in the amplification of nucleic acids. The invention also relates to an improved sample port for introducing a volume of a liquid sample into a continuous flow system.
    Type: Grant
    Filed: February 2, 2007
    Date of Patent: February 28, 2012
    Assignee: Corbett Life Science Pty Ltd
    Inventors: Keith Stanley, John Corbett
  • Patent number: 8101137
    Abstract: An analysis instrument for processing a microfluidic device, having sample storage means, a microfluidic device holder, sample loading means for loading sample into a microfluidic device disposed in the holder, processing means for enabling a reaction in a microfluidic device, and detection means for detecting and/or measuring the reaction is disclosed. The microfluidic device holder is adapted to hold the microfluidic device including a tape in position for processing and/or detection. A microfluidic processing device is also disclosed which includes a reaction chamber, and a sample loading chamber into which a sample is injectable. The reaction chamber is operatively connected to the sample loading chamber. A cover extends across at least part of the sample loading chamber. The cover and the reaction chamber include pierceable material and are separated by an overspill cavity configured to accept any overspill of an injected sample.
    Type: Grant
    Filed: February 8, 2006
    Date of Patent: January 24, 2012
    Assignee: Lab901 Limited
    Inventors: Stuart Polwart, Joel Fearnley, Kenneth G. Macnamara, Urs Lamb
  • Patent number: 8097153
    Abstract: A device, system and method for exchanging components between first and second fluids by direct contact in a microfluidic channel. The fluids flow as thin layers in the channel. One of the fluids is passed through a filter upon exiting the channel and is recycled through a secondary processor which changes the fluid's properties. The recycled fluid is reused for further exchange. The filter excludes blood cells from the recycled fluid and prevents or limits clogging of the filter. The secondary processor removes metabolic waste and water by diafiltration.
    Type: Grant
    Filed: April 13, 2010
    Date of Patent: January 17, 2012
    Assignee: The Trustees of Columbia in the City of New York
    Inventors: Edward F. Leonard, Alan C. West, Christian Paul Aucoin, Edgar E. Nanne
  • Patent number: 8083706
    Abstract: The present invention is directed to devices, systems and methods for removing undesirable materials from a sample fluid by contact with a second fluid. The sample fluid flows as a thin layer adjacent to, or between, concurrently flowing layers of the second fluid, without an intervening membrane. In various embodiments, a secondary separator is used to restrict the removal of desirable substances and effect the removal of undesirable substances from blood. The invention is useful in a variety of situations where a sample fluid is to be purified via a diffusion mechanism against an extractor fluid. Moreover, the invention may be used for the removal of components from a sample fluid that vary in size. When blood is the sample fluid, for example, this may include the removal of ‘small’ molecules, ‘middle’ molecules, macromolecules, macromolecular aggregates, and cells, from the blood sample to the extractor fluid.
    Type: Grant
    Filed: July 7, 2009
    Date of Patent: December 27, 2011
    Assignee: The Trustees of Columbia University in the City of New York
    Inventors: Edward F. Leonard, Alan C. West, Nina C. Shapley, Zhongliang Tang
  • Patent number: 8062903
    Abstract: Methods and devices are provided for overcoming detrimental diffusive effects in a sample liquid stream by forming segmented liquid bodies (e.g., droplets) from a sample liquid stream in an immiscible liquid stream. The liquid bodies are formed at the intersection of a channel providing the sample liquid stream and a channel providing the immiscible liquid stream. The formed liquid bodies compartmentalize the portion of the sample liquid stream from which the liquid bodies are formed, thus minimizing the detrimental effects of diffusion that occur in a continuous liquid stream.
    Type: Grant
    Filed: March 3, 2009
    Date of Patent: November 22, 2011
    Assignee: University of Washington
    Inventors: Daniel T. Chiu, John Scott Edgar
  • Patent number: 8052930
    Abstract: A device for characterizing ex vivo tissue employs a set of independent electrodes that may be used to scan the tissue by moving a voltage gradient across the tissue surface acquiring impedance spectrographs that may be mapped to an image.
    Type: Grant
    Filed: April 2, 2009
    Date of Patent: November 8, 2011
    Assignee: WiSys Technology Foundation
    Inventor: William D. Gregory
  • Patent number: 8043846
    Abstract: The invention features devices for mixing fluids, e.g., for lysing cells, and methods of use thereof. One device is based on the ability to control the flow of fluids, e.g., by contact angle and channel size. Fluids in this device can be divided to form segments of controlled volume, which are then brought together to initiate mixing. An exemplary use of the device is for the lysis of single cells. Another device is based on the ability to two mix two fluids in a channel and affinity capture of analytes. The devices can be integrated on the same chip with other devices, for example, for cell handling or analysis of DNA, mRNA, and proteins released from the lysis of a cell.
    Type: Grant
    Filed: June 9, 2004
    Date of Patent: October 25, 2011
    Assignee: The General Hospital Corporation
    Inventors: Daniel Irimia, Mehmet Toner
  • Patent number: 8030057
    Abstract: Method and device for storing and/or delivering fluids, wherein at least a first and a second fluid, such as chemical or biochemical reagents or rinse solutions, are maintained separately from each other in a common vessel and transferred in series from the vessel to a reaction site to carry out a predetermined chemical or biochemical reaction. Separation may be achieved by interposing a third fluid, e.g., a gaseous fluid plug, between the first and second fluids.
    Type: Grant
    Filed: January 26, 2005
    Date of Patent: October 4, 2011
    Assignee: President and Fellows of Harvard College
    Inventors: Vincent Linder, Samuel K. Sia, George M. Whitesides
  • Patent number: 8025853
    Abstract: A biochemical processing apparatus is provided having a stage receiving a biochemical reaction cartridge which includes chambers and flow paths communicating therebetween, a moving system for moving liquid via the flow paths, and a detector for detecting the presence of the liquid in a chamber and/or the amount of the liquid. In addition, a determining device determines a result of the movement of the liquid from the information of the liquid in the chamber detected by the detector.
    Type: Grant
    Filed: December 7, 2005
    Date of Patent: September 27, 2011
    Assignee: Canon Kabushiki Kaisha
    Inventor: Yasuyuki Numajiri
  • Patent number: 8021884
    Abstract: A system and method for detecting the presence of submicron sized particles in a sample taken from the environment. More particularly, the system may be used to detect and identify bacteria by detecting the presence of bacterial pili which have been separated from bacterial cells in the sample. The system includes means for collecting a sample from the environment, separating pili from bacteria in the sample, and purifying and concentrating the submicron sized pili in the sample based on the size of the pili. The purified and concentrated pili are detected with an apparatus which includes an electrospray assembly having an electrospray capillary, a differential mobility analyzer which receives output from the capillary, and a condensation particle counting device for counting the number of pili sized particles that pass through the differential mobility analyzer.
    Type: Grant
    Filed: December 9, 2010
    Date of Patent: September 20, 2011
    Assignee: The United States of America as represented by the Secretary of the Army
    Inventor: Charles H. Wick
  • Patent number: 8007739
    Abstract: Protein crystallization screening and optimization droplet actuators, systems and methods are provided. According to one embodiment, a screening droplet actuator is provided and includes: (a) a port for introduction of one or more crystallization reagents and/or one or more protein solutions; and (b) a substrate including: (i) an array of two or more mixing wells; and (ii) electric field mediated microfluidics for moving droplets comprising the crystallization reagents and protein solutions into the mixing wells. Optimization droplet actuators, systems including screening droplet actuators, methods of screening protein crystallization conditions, and methods of testing conditions for growing a crystal are also provided.
    Type: Grant
    Filed: August 16, 2007
    Date of Patent: August 30, 2011
    Assignees: Advanced Liquid Logic, Inc., Duke University
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Allen E. Eckhardt, Vijay Srinivasan
  • Patent number: 8003054
    Abstract: Fluid analyzer systems are provided which can detect a multitude of fluids in a sample to a very high level of accuracy which includes the detection of the presence of very small amounts of fluids. The results are both qualitative and quantitative. The systems consist of a receptacle which is filled with the fluid sample to be analysed which is placed into a consistent light condition environment where its temperature is measured. Under a predetermined time duration, Charge-Coupled Device (CCD) detector(s) receive by absorbance, radiation from the fluid sample at known wavelengths and are matched against a databank of known wavelengths of fluids. Matching wavelengths within a pre-defined tolerance will determine whether an individual fluid is present or not.
    Type: Grant
    Filed: August 25, 2009
    Date of Patent: August 23, 2011
    Assignee: Elan Vital (UK) Ltd
    Inventors: Timothy Nicholas Moor, Jonathan Alexander Redecen Dibble
  • Patent number: 7998436
    Abstract: Multiwell droplet actuators, systems and methods are provided. According to one embodiment, a substrate is provided and comprises: (a) one or more input ports for introduction of one or more reagents and/or samples; (b) a regular array of processing wells; and (c) a network of droplet transport pathways comprising pathways that provide direct or indirect droplet transport from each of the input ports to each of the one or more processing wells. Varying droplet actuators and systems related thereto are also provided.
    Type: Grant
    Filed: August 16, 2007
    Date of Patent: August 16, 2011
    Assignee: Advanced Liquid Logic, Inc.
    Inventors: Michael G. Pollack, Vamsee K. Pamula, Vijay Srinivasan
  • Patent number: 7993934
    Abstract: A reaction method using a microreactor, comprises: forming, in a microchannel, an at least two-layered laminar flow in n kinds (n is an integer of 3 or more) of a fluid 1, a fluid 2, . . . , and a fluid n at least two kinds of which are mutually incompatible; and pulsating at least one kind of the fluids.
    Type: Grant
    Filed: August 20, 2008
    Date of Patent: August 9, 2011
    Assignee: Fuji Xerox Co., Ltd.
    Inventors: Kazuaki Tabata, Masaki Hirota, Takayuki Yamada
  • Patent number: 7955863
    Abstract: Microfluidic devices for manipulating relatively dense materials, such as colloidal rod particles, are provided. Microfluidic devices for separating a denser first material from a less-dense second material are provided. Methods of manipulating a relatively dense first material, for example, colloidal rod particles, and separating the first material from a less-dense second material, are provided. Methods of marking samples or sample components with relatively dense materials, are also provided.
    Type: Grant
    Filed: February 11, 2008
    Date of Patent: June 7, 2011
    Assignee: Applied Biosystems, LLC
    Inventor: David Cox
  • Patent number: 7935489
    Abstract: Methods for detecting one or more analytes, such as a protein, in a fluid path are provided. The methods include resolving, immobilizing and detecting one or more analytes in a fluid path, such as a capillary. Also included are devices and kits for performing such assays.
    Type: Grant
    Filed: July 19, 2005
    Date of Patent: May 3, 2011
    Assignee: Cell Biosciences, Inc.
    Inventors: Roger A. O'Neill, Marc Glazer, Tom Weisan Yang
  • Patent number: 7935308
    Abstract: Methods for detecting one or more analytes, such as a protein, in a fluid path are provided. The methods include resolving, immobilizing and detecting one or more analytes in a fluid path, such as a capillary. Also included are devices and kits for performing such assays.
    Type: Grant
    Filed: October 30, 2007
    Date of Patent: May 3, 2011
    Assignee: Cell Biosciences, Inc.
    Inventors: Roger A. O'Neill, Marc Glazer, Tom Weisan Yang
  • Patent number: 7935479
    Abstract: Methods for detecting one or more analytes, such as a protein, in a fluid path are described herein. In some embodiments a method includes resolving one or more analytes in a fluid path, such as, for example, a capillary. After the one or more analytes are resolved, the one or more analytes are bound to the fluid path upon activation of one or more triggerable agents disposed within the fluid path. The one or more analytes that are bound to the fluid path are detected within the fluid path.
    Type: Grant
    Filed: March 19, 2007
    Date of Patent: May 3, 2011
    Assignee: Cell Biosciences, Inc.
    Inventors: Roger A. O'Neill, Marc Glazer, Tom W. Yang, Daniel J. Suich, Karl O. Voss
  • Patent number: 7922885
    Abstract: In a device for transporting liquid, liquid to be transported is enclosed by an oil droplet, which is fractionated with air, for manipulation. Moreover, as a method for supplying liquids wherein a plurality of liquids are enclosed by an oil droplet sequentially and treated, liquid enclosed by an oil droplet is formed by associating one oil with one liquid in a liquid introducing section. According to the present invention, even in the case where a plurality of liquids are manipulated, it is possible to manipulate stably without affecting other liquids. Moreover, it is possible to treat as a liquid enclosed by an oil droplet successively in the device for transporting liquid, thereby allowing the invention to be applied to a system for analyzing easily.
    Type: Grant
    Filed: May 10, 2006
    Date of Patent: April 12, 2011
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Sakuichiro Adachi, Kunio Harada, Hideo Enoki, Isao Yamazaki
  • Patent number: 7910379
    Abstract: Disclosed is an apparatus and method for ejecting droplets using charge concentration and liquid bridge breakup. The droplet ejection apparatus includes a reservoir storing a liquid; a capillary nozzle having a lower end submerged in the liquid stored in the reservoir and an upper end exposed outside the surface of the liquid, the capillary nozzle transferring the liquid to the upper end using capillary force; a potentiostat for applying a voltage to the liquid; a substrate mount on which a substrate is disposed to face the upper end of the capillary nozzle; and a distance adjusting unit for reciprocatingly moving the substrate between first and second positions with respect to the capillary nozzle, wherein the first position denotes a position where a distance between the upper end of the capillary nozzle and the surface of the substrate is less than a effective distance.
    Type: Grant
    Filed: September 23, 2010
    Date of Patent: March 22, 2011
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Beom-seok Lee, Jeong-gun Lee, Hye-jung Cho, In-seok Kang, Jae-suk Moon, Jin-seok Hong
  • Patent number: 7910380
    Abstract: The present invention relates to a structure (a solution introducing structure) for introducing a plurality of solutions (a sample and/or a reagent solution) in highly accurate volume, in high amount and simply, into a channel, in particular a channel of a micro fluidic device, a micro fluidic device having said structure, a method for introducing a solution using said device, a method for separation of a substance, and a method for separating a complex between an analyte or analogue thereof, and a substance binding to said analyte or analogue thereof (hereinafter may be abbreviated as a complex forming substance or CFS) which is obtained by reacting a plurality of solutions introduced by these, and said CFS or analogue not involved in formation of said complex, rapidly, simply and in high accuracy, along with a method for measuring an analyte or an analogue thereof in a sample in high sensitivity.
    Type: Grant
    Filed: October 7, 2010
    Date of Patent: March 22, 2011
    Assignee: Wako Pure Chemical Industries, Ltd.
    Inventors: Tomohisa Kawabata, Shinji Satomura, Henry Garrett Wada
  • Patent number: 7906074
    Abstract: The system and method of the present application is a microfluidic array system for biological, chemical and biochemical assessments including a microfluidic chip for reaction assays, wherein the microfluidic chip includes a control layer and a fluidic layer, wherein the control layer is pressurized through pneumatic or hydraulic means in order to control the flow of the reagents in the fluidic layer. The system and method of the present application further includes a method of fabricating such a microfluidic chip, and further a method for operating the same. Lastly, the system and method of the present application further includes a system for operating and analyzing the microfluidic array including a pressure source, a fluidic source, a biochip reader, and a processor configured to control the same.
    Type: Grant
    Filed: April 9, 2008
    Date of Patent: March 15, 2011
    Assignee: Auburn University
    Inventors: Jong Wook Hong, Jihyun F. Kim, Tae Kwang Oh, Sung Ho Yoon
  • Patent number: 7892857
    Abstract: The invention relates to an assay method and a fluid microsystem for carrying out said assay method, in particular, for carrying out miniaturized affinity tests in a micro-array format. According to said invention, a liquid phase comprising at least one type of detectable high-molecular weight soluble substance, for example a labeled reaction partner or product of an affinity reaction is displaced by the hydraulic effect of a high-molecular weight osmotic agent on an ultrafiltration membrane towards a miniaturized measuring chamber defined thereby. The fraction of the detectable high-molecular weight substance(s) is concentrated in the measuring chamber, while the dissolved low-molecular weight components are removed with the solvent through the membrane pores, thereby making it possible to attain a high detection sensitivity.
    Type: Grant
    Filed: August 30, 2007
    Date of Patent: February 22, 2011
    Inventors: Rudolf Ehwald, Helge Adleff, Frank Bier, Nenad Gajovic-Eichelmann, Max Ehwald
  • Patent number: 7887753
    Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.
    Type: Grant
    Filed: May 27, 2008
    Date of Patent: February 15, 2011
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Marc A. Unger, Hou-Pu Chou, Todd A. Thorsen, Axel Scherer
  • Patent number: 7833804
    Abstract: The present invention relates to a solution introducing structure for introducing a plurality of solutions (a sample and/or a reagent solution) in highly accurate volume, in high amount and simply, into a channel (1) of a micro fluidic device, said channel (1) comprising not less than three side channels (2) for drain and not less than two side channels (3) for introducing a sample or a reagent solution.
    Type: Grant
    Filed: April 11, 2007
    Date of Patent: November 16, 2010
    Assignee: Wako Pure Chemical Industries, Ltd.
    Inventors: Tomohisa Kawabata, Shinji Satomura, Henry Garrett Wada
  • Patent number: 7811521
    Abstract: A testing microchip includes a specimen storage section; a reagent storage section; a reaction section; a testing section for a test of a reaction product obtained from the reaction; a liquid feed control section; and a gas bubble trapping structure. The sections are connected continuously by a series of flow channels. The liquid feed control section stops passing of liquid until a liquid feeding pressure reaches a predetermined pressure, and passes the liquid when the liquid feeding pressure becomes higher than the predetermined pressure; and the gas bubble trapping structure traps a gas bubble in the liquid that flows in the flow channel so that the gas bubble does not flow to the downstream side and only the liquid passes to the downstream side. A testing apparatus that performs testing in the testing section of the testing microchip, wherein the testing microchip is attachably and detachably mounted to the apparatus.
    Type: Grant
    Filed: March 21, 2006
    Date of Patent: October 12, 2010
    Assignee: Konica Minolta Medical & Graphic, Inc.
    Inventors: Yasuhiro Sando, Akihisa Nakajima, Kusunoki Higashino
  • Publication number: 20100240142
    Abstract: A solution component 18a separated in a separating cavity 23 is connected to a measuring cavity through a connecting channel 37 and a measurement channel 38, and a first capillary cavity 33 is provided on one side of the separating cavity 23 so as to communicate with the connecting channel 37. The first capillary cavity 33 is formed to extend to the outside of a separation interface 18c of a sample liquid separated in the separating cavity 23.
    Type: Application
    Filed: November 7, 2008
    Publication date: September 23, 2010
    Applicant: Panasonic Corporation
    Inventors: Hiroshi Saiki, Kouzou Tagashira, Tomohiro Kijima, Hirofumi Sugimoto, Kenji Watanabe, Hisashi Takahashi, Masaaki Kitoh