And Means Segmenting Fluid Material Patents (Class 422/82)
  • Patent number: 7799606
    Abstract: An ion-sensitive sensor arrangement includes: a semiconductor chip having a first surface, which has a media-sensitive region and at least one, first, electrical contact surface; and a support having a second surface, which faces the first surface of the semiconductor chip. An opening is provided, which aligns with the sensitive region, and at least one, second, electrical contact surface, which overlaps, or aligns with, the at least one, first, electrical contact surface. Between the support and the semiconductor chip, a preferably elastic, anisotropic conductor is arranged, which produces a conducting connection between the at least one, first, contact surface and the at least one, second, contact surface, and which has a traversing opening, which aligns with the opening, so that the sensitive region of the semiconductor chip can be contacted through the opening by an analyte. The preferably elastic, anisotropic conductor seals the region outside of the opening against contamination with the analyte.
    Type: Grant
    Filed: December 6, 2003
    Date of Patent: September 21, 2010
    Assignee: Endress + Hauser Conducta Gesellschaft fur Mess- u. Regeltechnik mbH + Co. KG
    Inventors: Torsten Pechstein, Robert Scholz
  • Patent number: 7795037
    Abstract: Methods for using semiconductor nanocrystals for determining fluid movement, fluid dilution and fluid removal are described. Methods for using semiconductor nanocrystals for monitoring and quantifying the amounts of solid materials dissolved in a liquid are also described.
    Type: Grant
    Filed: June 6, 2008
    Date of Patent: September 14, 2010
    Assignee: Novartis Vaccines and Diagnostics, Inc.
    Inventors: Willy Lagwinski, Charles Harrington, Bruce Phelps
  • Patent number: 7785535
    Abstract: A sample processing cartridge may include a plurality of segments arranged in an array at least two rows long and two columns wide. Each segment may be defined by at least one wall of the sample cartridge, fluidly isolated from adjacent segments at least in part by at least one breakable seal or by at least one permanent seal, so expandable as to receive a volume of fluid expelled from another segment, and so compressible as to contain substantially no fluid when so compressed. At least two adjacent segments of at least one row of the array may be aligned along a longitudinal axis of the row and have substantially the same height along a latitudinal axis of the row. At least two adjacent segments in at least one row may be separated by a permanent seal to form at least two tracks. At least one segment, or at least two adjacent segments separated by a breakable seal, may be in fluid communication with the at least two tracks. At least one segment may contain at least one reagent.
    Type: Grant
    Filed: June 7, 2005
    Date of Patent: August 31, 2010
    Assignee: IQuum, Inc.
    Inventors: Shuqi Chen, Bertrand Lemieux, Lingjun Chen
  • Publication number: 20100216126
    Abstract: A microfluidic device comprising; i) an inlet; ii) a first layer comprising at least first and second current carrying structures, wherein the at least first and second current carrying structures each comprise a plurality of teeth, and wherein the teeth of the first and second current carrying structures are optionally offset such that the teeth of the first current carrying structure are positioned between the teeth of the second current carrying structure; iii) a second layer comprising a first microfluidic chamber in fluid communication with the inlet positioned above the at least first and second current carrying structures of the first layer; and iv) a third layer comprising at least third and fourth current carrying structures wherein the at least third and fourth current carrying structures each comprise a plurality of teeth, and wherein the teeth of the third and fourth current carrying structures are optionally offset such that the teeth of the third current carrying structure are positioned between
    Type: Application
    Filed: January 12, 2008
    Publication date: August 26, 2010
    Applicant: BRUNEL UNIVERSITY
    Inventors: Wamadeva Balachandran, Sayyed Mohamad Azimi, Jeremy Ahern, Massoud Zolgharni, Mohammad Reza Bahmanyar, Predrag Slijepcevic
  • Publication number: 20100210019
    Abstract: The present invention is to present a sample analyzer comprising: a reagent container holder; a measurement unit; and an information processing unit configured to perform operations comprising: controlling the measurement unit so as to start the successive measurement of the plurality of samples; determining whether or not to switch from the first reagent container to the second reagent container while performing the successive measurement by using the reagent contained in the first reagent container; controlling the measurement unit so as to suspend a start of aspiration of a next sample, to measure a quality control measurement sample prepared from the reagent contained in the second reagent container, when determined to switch from the first reagent container to the second reagent container; and controlling the measurement unit so as to start the aspiration of the next sample when an analysis result of the quality control measurement sample meets a predetermined condition.
    Type: Application
    Filed: February 16, 2010
    Publication date: August 19, 2010
    Applicant: SYSMEX CORPORATION
    Inventors: Hiroshi KURONO, Keitarou Kondou
  • Publication number: 20100196205
    Abstract: A fluidic configuration, both structural and methodological, for the injection of sample greatly reduces dead volume allowing rapid transition to 100% sample in a flow cell. For a continuous flow injection analysis system the structure and method provide counter flows to remove in one direction the dispersed region of the sample to waste before injecting non-dispersed sample into the flow cell by reversing the effective flow direction. The injection point itself is directly adjacent to the flow cell where all channels are microfluidic channels. Therefore, only the flow cell volume needs to be displaced during injection of sample in order to achieve 100% transition to sample within the flow cell. This greatly accelerates the rise and fall times thereby extending the kinetic range of the real-time interaction analysis instrument. In addition such rapid transition to sample improves overall data quality thereby improving kinetic model fitting.
    Type: Application
    Filed: January 16, 2008
    Publication date: August 5, 2010
    Inventor: John Gerard Quinn
  • Publication number: 20100190263
    Abstract: The present invention is directed to a droplet actuator and methods of making and using the droplet actuator including one or more substrates configured to form a droplet operations gap and including a physical or chemical feature that may be provided at a predetermined locus within or exposed to the droplet operations gap and configured to retain a bubble in position within the droplet operations gap.
    Type: Application
    Filed: January 25, 2010
    Publication date: July 29, 2010
    Applicant: Advanced Liquid Logic, Inc.
    Inventors: Vijay Srinivasan, Michael G. Pollack, Arjun Sudarsan, Zhishan Hua
  • Patent number: 7754150
    Abstract: Methods of detecting a component of interest, such as a protein, in a microfluidic system are provided. The methods include the use of a component-binding moiety specific to the component of interest, such as an antibody, to detect the component of interest. Also included are microfluidic devices and integrated systems for performing such assays, including devices utilizing flowable or fixed particle sets.
    Type: Grant
    Filed: July 2, 2003
    Date of Patent: July 13, 2010
    Assignee: Caliper Life Sciences, Inc.
    Inventors: H. Garrett Wada, Matthew B. Murphy
  • Patent number: 7749365
    Abstract: The invention herein provides improved sample injection systems and related methods to create microfluidic devices with symmetrical channel configurations that can produce relatively large sample volumes. An embodiment of the invention provides microfluidic structures with different geometries that are symmetrical from the perspective of a sample load channel and a sample waste channel, which essentially eliminates issues of time offset and other problems commonly associated with twin-T sample formation techniques. A split-injection approach and related methods of sample plug formation are therefore provided.
    Type: Grant
    Filed: July 26, 2006
    Date of Patent: July 6, 2010
    Assignee: IntegenX, Inc.
    Inventors: Michael Nguyen, Luc Bousse
  • Patent number: 7731902
    Abstract: The present invention provides a sensor device for detecting a substance contained in a fluid comprising a first photonic crystal region, a second photonic crystal region, a first channel which is connected to the first photonic crystal region, and passes the fluid containing the substance therein, a second channel which is connected to the second photonic crystal region, and passes a fluid for reference therein, an optical waveguide which is connected to the first photonic crystal region and the second photonic crystal region, and guides light, and a detecting section for detecting a difference between a light which has passed through the optical waveguide and the first photonic crystal region and a light which has passed through the optical waveguide and the second photonic crystal region.
    Type: Grant
    Filed: February 21, 2006
    Date of Patent: June 8, 2010
    Assignee: Canon Kabushiki Kaisha
    Inventors: Yasuhiro Nagatomo, Mitsuro Sugita, Mamoru Uchida
  • Patent number: 7704748
    Abstract: A novel Flame Temperature Analyzer (FTA) method and apparatus for measuring combustible gas concentration and oxygen content in a sample gas includes supplying a mixture of oxidant and fuel to a sensing flame and measuring the temperature of the flame as the sample is added to the combustion chamber.
    Type: Grant
    Filed: June 26, 2003
    Date of Patent: April 27, 2010
    Assignee: Control Instruments Corporation
    Inventors: Christopher G. Schaeffer, Matthew James Schaeffer, Pravin Patel
  • Patent number: 7691329
    Abstract: In one embodiment, a method for detecting contaminants in a liquid comprises: contacting a sensor with a liquid, generating electrical information based upon a concentration of the contaminant in the liquid, transmitting the electrical information to a controller, and determining the concentration of a contaminant in the liquid. The sensor can comprise a film, a purge chamber, and a transducer, which are configured such that a first surface of the film is in fluid communication with the liquid and the purge chamber is in fluid communication with a second surface of the film that is opposite the first surface, and wherein the transducer is in fluid communication with the purge chamber.
    Type: Grant
    Filed: November 16, 2006
    Date of Patent: April 6, 2010
    Assignee: General Electric Company
    Inventors: Radislav Alexandrovich Potyrailo, David James Monk, Ryo Tamaki
  • Patent number: 7674615
    Abstract: A structure for controlling fluid flow from a sample receiving pad into a lateral flow assay test strip, including: a lateral flow assay test strip; a sample pad abutting the lateral flow assay test strip; a pinch wall positioned to direct fluid flow from the sample pad to the lateral flow assay test strip; a top support structure positioned on top of the test strip; and a bottom support structure positioned underneath the test strip, wherein each of the top and bottom support structures comprise a plurality of separate spaced-apart support ribs positioned along the length of the test strip.
    Type: Grant
    Filed: May 4, 2005
    Date of Patent: March 9, 2010
    Assignee: Bayer HealthCare LLC
    Inventors: Urs A. Ramel, Dillan Tay
  • Patent number: 7670557
    Abstract: A planar-sensor arrangement is disclosed, which is used to detect particles possibly contained in an analyte. The sensor element includes a substrate, a first planar-sensor electrode and a second planar-sensor electrode which are formed on and/or in the substrate and whereon catcher molecules can be immobilized. The first sensor-electrode and the second sensor-electrode are divided, respectively, into a plurality of planar sensor-electrode partial areas. The sensor electrode partial areas of the first sensor electrode and the sensor electrode partial areas of the second sensor electrode are arranged in an alternating manner in two dimensions on the surface plane of the substrate. The sensor element also includes a wiring structure by which at least one part of the sensor electrode partial areas of the first sensor electrode are electrically coupled together, and by which at least one part of the sensor-electrode partial areas of the second sensor electrode are electrically coupled together.
    Type: Grant
    Filed: June 30, 2005
    Date of Patent: March 2, 2010
    Assignee: Siemens Aktiengesellschaft
    Inventors: Alexander Frey, Christian Paulus, Meinrad Schienle, Roland Thewes
  • Patent number: 7662309
    Abstract: The present invention relates to a class of luminescent and conductive polymer compositions having chromophores, and particularly solid films of these compositions exhibiting increased luminescent lifetimes, quantum yields and amplified emissions. These desirable properties can be provided through polymers having rigid groups designed to prevent polymer reorganization, aggregation or ?-stacking upon solidification. These polymers can also display an unusually high stability with respect to solvent and heat exposures. The invention also relates to a sensor and a method for sensing an analyte through the luminescent and conductive properties of these polymers. Analytes can be sensed by activation of a chromophore at a polymer surface. Analytes include aromatics, phosphate ester groups and in particular explosives and chemical warfare agents in a gaseous state.
    Type: Grant
    Filed: October 17, 2005
    Date of Patent: February 16, 2010
    Assignee: Massachusetts Institute of Technology
    Inventors: Timothy M. Swager, Jye-Shane Yang, Vance Williams, Yijun Miao, Claus G. Lugmair, Igor A. Levitsky, Jinsang Kim, Robert Deans
  • Publication number: 20090325300
    Abstract: A method of rapidly detecting trace materials including biohazards, toxins, radioactive materials, and narcotics in situ is disclosed. A corresponding apparatus is disclosed. A trace of the material is collected on a pad of the card component, collected by swiping the pad on suspected surface or exposure to the suspected air volume. A novel card component is disclosed that when inserted in a chemical detection unit (CDU), releases reaction chemicals from flexible walled capsules in desired sequence. The exposed pad containing trace material and chemicals are heated in the chemical detection unit to produce a spectral pattern that is analyzed by the optical electronics in the CDU and results are displayed, stored and/or transmitted over a communications network.
    Type: Application
    Filed: July 25, 2007
    Publication date: December 31, 2009
    Inventors: Vaughan L. Clift, Kristin L. Harriman, Richard L. Pettys, David M. Headley, Tiffany M. Conerly
  • Patent number: 7610943
    Abstract: Systems and methods are provided for dispensing portions of viscous material, such as the viscous materials used to form crystals of membrane proteins or soluble proteins. A liquid under pressure is used to divide the viscous materials into portions. The divided viscous material portions can optionally be deposited into are receptacle.
    Type: Grant
    Filed: June 8, 2005
    Date of Patent: November 3, 2009
    Assignee: Emerald Biosystems, Inc.
    Inventors: Peter Nollert, Alexandrina Muntianu, Robert C. Haushalter, Dean Allyn Hopkins, Jr.
  • Patent number: 7594986
    Abstract: An apparatus and method for capillary zone electrophoresis includes a polyelectrolyte multilayer positioned in a capillary tube for analytical separations of macromolecules. The capillary comprises a passage defined by passage walls comprising fused silica. The polyelectrolyte multilayer is positioned within the passage adjacent the walls, and comprises an organic polyelectrolyte. The passage may further comprise nonporous silica particles coated with a multilayer including a plurality of polyelectrolyte layers. An apparatus includes a power supply having a positive electrode and a negative electrode for generating an electric field therebetween. The apparatus includes a capillary having a passage formed by passage walls and comprising therein a polyelectrolyte multilayer positioned substantially within the passage.
    Type: Grant
    Filed: October 18, 2007
    Date of Patent: September 29, 2009
    Assignee: Florida State University Research Foundation, Inc.
    Inventors: Joseph B. Schlenoff, Timothy W. Graul
  • Patent number: 7572409
    Abstract: Apparatus and method for handling biological samples. Segments of ionic liquid can provide voltage across segments of immiscible liquid to concentrate or separate charged species in the biological samples. Reactants in biological samples can be contacted and reacted in segments of immiscible liquid.
    Type: Grant
    Filed: May 23, 2003
    Date of Patent: August 11, 2009
    Assignee: Applied Biosystems, LLC
    Inventors: Zbigniew T. Bryning, Timothy Z. Liu
  • Patent number: 7556776
    Abstract: The present invention relates generally to microfluidic structures, and more specifically, to microfluidic structures and methods including microreactors for manipulating fluids and reactions. In some embodiments, structures and methods for manipulating many (e.g., 1000) fluid samples, i.e., in the form of droplets, are described. Processes such as diffusion, evaporation, dilution, and precipitation can be controlled in each fluid sample. These methods also enable conditions within the fluid samples (e.g., concentration) to be controlled. Manipulation of fluid samples can be useful for a variety of applications, including testing for reaction conditions, e.g., in crystallization, chemical, and biological assays.
    Type: Grant
    Filed: September 8, 2005
    Date of Patent: July 7, 2009
    Assignees: President and Fellows of Harvard College, Brandeis University
    Inventors: Seth Fraden, Darren Roy Link, Galder Cristobal-Azkarate, Jung uk Shim, David A. Weitz
  • Patent number: 7550267
    Abstract: An improved microscale diffusion immunoassay utilizing multivalent reactants is disclosed. In particular, a method for detecting the presence of analyte particles in an analyte fluid is disclosed, the method comprising: (a) providing the analyte fluid comprising the analyte particles; (b) providing a diffusion fluid comprising binding particles capable of binding with the analyte particles; (c) flowing the analyte fluid and the diffusion fluid in adjacent laminar flow through a microfluidic channel; (d) allowing the analyte particles to diffuse into the diffusion fluid and bind with the binding particles to form analyte/binding particle complexes; and (e) detecting the presence of the analyte particles and the analyte/binding particle complexes, wherein each of the binding particles is capable of binding with more than one analyte particle, and wherein each of the analyte particles is capable of binding with more than one binding particle.
    Type: Grant
    Filed: September 14, 2005
    Date of Patent: June 23, 2009
    Assignee: University of Washington
    Inventors: Kenneth R. Hawkins, Paul Yager
  • Patent number: 7524462
    Abstract: Apparatus and methods consistent with certain principles related to the present invention include a first channel, where a fluid flows through the first channel, where the first channel comprises an upstream region, a tagging region downstream of the upstream region, where at least one analyte is tagged when the fluid flows through the tagging region, a capture region downstream of the tagging region, where at least one tagged analyte is captured when the fluid flows through the capture region. A second channel communicating the upstream region of the first channel with the first channel intermediate the tagging region and capture regions, where a portion of the fluid flows through the second channel and subsequently flows into the capture region of the first channel, and where the fluid flowing through the tagging region flows into the capture region before the portion of the fluid flowing through the second channel flows into the capture region of the first channel.
    Type: Grant
    Filed: March 29, 2002
    Date of Patent: April 28, 2009
    Assignee: Agilent Technologies, Inc.
    Inventors: Leslie Leonard, Klaus Stefan Drese, Dirk Boecker
  • Patent number: 7507586
    Abstract: This invention is a method and device for use with multi-dimensional chromatography that utilizes partial modulation with multiple secondary retention times. An analyte-bearing sample is subjected to a first dimension of chromatography. Thereafter the separated analyte-bearing sample is diluted with a modulated second carrier such at the analyte-bearing sample is not stopped or its temperature altered. The secondary retention time period at which the modulator cycles is variable among multiple secondary retention time periods to prevent loss of resolution with less complex and/or low boiling point compounds and to prevent loss of data with more complex and/or higher boiling point compounds. The partially modulated analyte-bearing sample then feeds into a secondary column where the analyte-bearing sample is further separated.
    Type: Grant
    Filed: December 19, 2005
    Date of Patent: March 24, 2009
    Assignee: Valco Instruments Co., Inc.
    Inventor: Huamin Cai
  • Patent number: 7488452
    Abstract: The system comprises a flow through reaction vessel having an inlet, an outlet and a reaction zone intermediate said inlet and outlet. The system further comprises reagent feed means for introducing one or more reagents into said vessel, said one or more reagents forming one or more reaction products in said reaction zone. Said reagent feed means at least include a liquid feed means for introducing a liquid into said inlet of said vessel. The vessel has a liquid evaporation zone intermediate said inlet and said reaction zone. Said liquid evaporation zone comprises an evaporation structure contacting said liquid and providing a liquid residence time in said liquid evaporation zone improving evaporation of said liquid.
    Type: Grant
    Filed: May 13, 2002
    Date of Patent: February 10, 2009
    Assignee: Avantium International B.V.
    Inventors: Peter John van den Brink, Maarten Bracht
  • Patent number: 7485118
    Abstract: The present invention relates to a microstructure arrangement for the bubble-free filling with a liquid of at least one system for draining off liquids. The arrangement has an inlet for the arrangement to be connected to a system for the supply of liquids and at least one outlet for the arrangement to be connected to the at least one liquid-discharging system. The arrangement has a transition region, through which the liquid can be transported from the inlet to the at least one outlet. At a start of the transition region, at least one first microstructure element for producing a point with increased capillary force is provided, in order to achieve gap-free wetting of the areas bounding this point with increased capillary force, in particular side walls, a cover and/or a bottom.
    Type: Grant
    Filed: December 20, 2004
    Date of Patent: February 3, 2009
    Assignee: Boehringer Ingelholm Microparts GmbH
    Inventors: Gert Blankenstein, Ralf-Peter Peters, Claus Marquordt
  • Patent number: 7452726
    Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system.
    Type: Grant
    Filed: December 11, 2003
    Date of Patent: November 18, 2008
    Assignee: Fluidigm Corporation
    Inventors: Hou-Pu Chou, Antoine Daridon, Kevin Farrell, Brian Fowler, Yish-Hann Liau, Ian D. Manger, Hany Ramez Nassef, William Throndset
  • Patent number: 7438856
    Abstract: Embodiments of the invention are directed to microfluidic devices. In one embodiment, a microanalysis chip comprises a body having at least one transfer-separation channel with a channel bottom that has a bottom opening. The transfer-separation channel terminates in a discharge aperture.
    Type: Grant
    Filed: June 24, 2003
    Date of Patent: October 21, 2008
    Assignee: Zyomyx, Inc.
    Inventors: Paul Jedrzejewski, Steffen Nock, Peter Wagner, Pierre Indermuhle, Frank G. Zaugg
  • Patent number: 7431889
    Abstract: A microfluidic device comprising one, two, three or more enclosed microchannel structures, each of which comprises a) an inlet arrangement with an inlet opening for liquid, b) a reaction microcavity (RM1) downstream to the inlet opening, and c) an upstream section that is located between the inlet opening and the reaction microcavity (RM1) and is defined between two planar substrates made of plastic material which substrates are common for said microchannel structures. The characteristic feature is that the inner wall in said upstream section exposes a hydrophilic surface area with an underlying base layer comprising metal oxide. In one aspect the microfluidic device as such is covered.
    Type: Grant
    Filed: July 29, 2005
    Date of Patent: October 7, 2008
    Assignee: Gyros Patent AB
    Inventors: Johan Engstrom, Cecillia Hellermark
  • Patent number: 7431885
    Abstract: A measuring device for the amperometric measurement of test strips 12, each having a test field moistenable by a liquid to be investigated and having measuring electrodes associated with the test field. The device includes at least one test strip receiver 20 for the insertion of a test strip 12, first contact elements arranged in the region of the strip receiver 20 for contacting the measuring electrodes of a test strip 12 located in the strip receiver 20, and an evaluation and control circuit. The strip receiver 20 is formed with a separate unitary housing which on its external side has second external contact elements 50 electrically connected with the first internal contact elements. The external contact elements 50 are designed for contacting opposite contact elements 16 connected with the evaluation and control circuit.
    Type: Grant
    Filed: May 23, 2007
    Date of Patent: October 7, 2008
    Assignee: LRE Medical GmbH
    Inventors: Norbert Pöllmann, Wolfgang Heider, Genrich Siris
  • Patent number: 7416896
    Abstract: A method for determining total and bound plasma bilirubin. The method utilizes zone fluidics to enable accurate determination of the bilirubin using small volumes of sample and reagents, and significantly reduces dilution errors caused by inherent changes in bilirubin-binding with sample dilution, as well as the effects of weak bilirubin-binding competitors.
    Type: Grant
    Filed: December 20, 2004
    Date of Patent: August 26, 2008
    Assignees: Global FIA, Inc., LW Ligang LLC
    Inventors: Charles E. Ahlfors, Graham D. Marshall, Duane K. Wolcott
  • Patent number: 7416903
    Abstract: The present invention provides methods and apparatus for mixing samples in-line in a microfluidic system, comprising methods of and means for introducing a first fluid sample into a flow-tube at a first end at a first velocity via a first conduit; methods of and means for introducing a second fluid sample into the flow-tube at the first end at a second velocity, the second velocity different from the first velocity, via a second conduit, wherein the first fluid sample and the second fluid sample converge in the flow tube to form an interface; whereby the first fluid sample and the second fluid sample mix at the interface within the flow-tube, wherein fluid flow at the first end of the flow-tube is laminar and fluid flow at a second end of the flow-tube is laminar, and wherein the flow-tube has a constant diameter between the first end and the second end of the flow-tube.
    Type: Grant
    Filed: May 5, 2003
    Date of Patent: August 26, 2008
    Assignee: STC.UNM
    Inventors: Larry A. Sklar, Andrea A. Mammoli, Richard A. Truesdell, Peter V. Vorobieff
  • Patent number: 7393503
    Abstract: The present invention relates to a class of luminescent and conductive polymer compositions having chromophores, and particularly solid films of these compositions exhibiting increased luminescent lifetimes, quantum yields and amplified emissions. These desirable properties can be provided through polymers having rigid groups designed to prevent polymer reorganization, aggregation or ?-stacking upon solidification. These polymers can also display an unusually high stability with respect to solvent and heat exposures. The invention also relates to a sensor and a method for sensing an analyte through the luminescent and conductive properties of these polymers. Analytes can be sensed by activation of a chromophore at a polymer surface. Analytes include aromatics, phosphate ester groups and in particular explosives and chemical warfare agents in a gaseous state.
    Type: Grant
    Filed: October 17, 2005
    Date of Patent: July 1, 2008
    Assignee: Massachusetts Institute of Technology
    Inventors: Timothy M. Swager, Jye-Shane Yang, Vance Williams, Yijun Miao, Claus G. Lugmair, Igor A. Levitsky, Jinsang Kim, Robert Deans
  • Patent number: 7384602
    Abstract: In a chemical analysis apparatus for extracting a specific substance, such as nucleic acid, from a sample containing a plurality of chemical substances, solutions remaining in valves are prevented so that a reagent in an earlier step does not contaminate the subsequent steps. Predetermined quantities of solutions are carried and supplied by centrifugal force without providing valves for controlling the flow of the solutions. After perforating the lids of ventilation holes 272, 273, 274 communicated with a detection container 450 and disposal containers 460 and 470 in an analysis disc 2, the analysis disc 2 is rotated to supply and carry a predetermined quantity of the sample.
    Type: Grant
    Filed: March 28, 2003
    Date of Patent: June 10, 2008
    Assignee: Hitachi High-Technologies Corporation
    Inventors: Yoshihiro Nagaoka, Naruo Watanabe, Kei Takenaka, Tomoki Ohashi, Yuji Miyahara
  • Patent number: 7384795
    Abstract: Methods for using semiconductor nanocrystals for determining fluid movement, fluid dilution and fluid removal are described. Methods for using semiconductor nanocrystals for monitoring and quantifying the amounts of solid materials dissolved in a liquid are also described.
    Type: Grant
    Filed: September 5, 2003
    Date of Patent: June 10, 2008
    Assignee: Novartis Vaccines and Diagnostics, Inc.
    Inventors: Willy Lagwinski, Charles A. Harrington, Bruce H. Phelps
  • Patent number: 7378280
    Abstract: The present invention provides microfluidic devices and methods for using the same. In particular, microfluidic devices of the present invention are useful in conducting a variety of assays and high throughput screening. Microfluidic devices of the present invention include elastomeric components and comprise a main flow channel; a plurality of branch flow channels; a plurality of control channels; and a plurality of valves. Preferably, each of the valves comprises one of the control channels and an elastomeric segment that is deflectable into or retractable from the main or branch flow channel upon which the valve operates in response to an actuation force applied to the control channel.
    Type: Grant
    Filed: November 16, 2001
    Date of Patent: May 27, 2008
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Marc A. Unger, Hou-Pu Chou, Todd A. Thorsen, Axel Scherer
  • Patent number: 7368084
    Abstract: The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.
    Type: Grant
    Filed: February 25, 2005
    Date of Patent: May 6, 2008
    Assignee: STC.UNM
    Inventors: Larry A. Sklar, Bruce S. Edwards, Frederick W. Kuckuck
  • Patent number: 7351376
    Abstract: The invention relates to a microfabricated device for the rapid detection of DNA, proteins or other molecules associated with a particular disease. The devices and methods of the invention can be used for the simultaneous diagnosis of multiple diseases by detecting molecules (e.g. amounts of molecules), such as polynucleotides (e.g., DNA) or proteins (e.g., antibodies), by measuring the signal of a detectable reporter associated with hybridized polynucleotides or antigen/antibody complex. In the microfabricated device according to the invention, detection of the presence of molecules (i.e., polynucleotides, proteins, or antigen/antibody complexes) are correlated to a hybridization signal from an optically-detectable (e.g. fluorescent) reporter associated with the bound molecules.
    Type: Grant
    Filed: November 28, 2000
    Date of Patent: April 1, 2008
    Assignee: California Institute of Technology
    Inventors: Stephen R. Quake, Hou-Pu Chou
  • Patent number: 7351587
    Abstract: A process for the on-line determination of the hydrogen peroxide content of a mixture obtained in a chemical reaction comprises at least the following steps: (1) admixing the mixture comprising hydrogen peroxide with at least one reagent which is capable of forming a substance which can be detected by optical methods on reaction with hydrogen peroxide, so as to form this substance, (2) determining the amount of the substance present by measuring its specific absorption in an appropriate wavelength range.
    Type: Grant
    Filed: February 5, 2002
    Date of Patent: April 1, 2008
    Assignee: BASF Aktiengesellschaft
    Inventors: Thomas Beuermann, Joaquim Henrique Teles
  • Patent number: 7341872
    Abstract: A vessel arrangement having a base and multiple vessels suited for simultaneously conducting a plurality of isolated experimental reactions or treatments at atmospheric process conditions or elevated temperatures and pressure condition has been developed. A component of a first material is introduced into one independent vessel through an opening in its top of the first vessel and another component of a second material is introduced into a different independent vessel through its top. Both vessels are removably located about a base at different first locations. Transformation of the components in the vessels then occurs to produce different materials therein. After completion of the experiments a displacement medium simultaneously urges the vessels from their respective locations about the base for discard or reuse after any necessary cleaning. Typically at least one property of the materials from the vessels is determined either within the vessel or after recovery of the materials.
    Type: Grant
    Filed: April 29, 2004
    Date of Patent: March 11, 2008
    Assignee: UOP LLC
    Inventors: Brent J. Anderson, Ralph D. Gillespie, Maureen L. Bricker
  • Patent number: 7338796
    Abstract: Disclosed is an apparatus and method for inserting one or several chemical or biological species into phospholipid containers that are controlled within a microfluidic network, wherein individual containers are tracked and manipulated by electric fields and wherein the contained species may be chemically processed.
    Type: Grant
    Filed: August 9, 2004
    Date of Patent: March 4, 2008
    Assignee: Sandia Corporation
    Inventors: Rafael V. Davalos, Christopher R. B. Ellis
  • Patent number: 7338804
    Abstract: This invention significantly reduces the frequency of hydraulic failure in the analytical line of a capsule chemistry sample liquid analysis system which can occur when the reagents required for the specific analytical test have an imperfect merger with the sample aliquot reagent in the vanish zone of the analytical line. It has been found that the dynamic surface tension of the sample aliquot reagent and the analytical reagent influence the merging process. It has also been found that optimum merging of these reagent capsules in the vanish zone can be obtained when the dynamic surface tension of the sample aliquot reagent and analytical reagent is different.
    Type: Grant
    Filed: November 24, 2004
    Date of Patent: March 4, 2008
    Assignee: Siemens Healthcare Diagnostics Inc.
    Inventors: Johan Kijlstra, Dieter Rüehle, Ralf Niegl, Paul Gherson, Germaine Adriana Tarcu
  • Patent number: 7329545
    Abstract: In a method for sampling a continuous liquid flow, the liquid flow is supplied to a surface along an input flow path. The liquid flow is sampled by forming a sample droplet from a portion of the liquid flow. The sample droplet is moved along an analysis flow path to a processing area of the surface, where the sample droplet is processed. Discrete sample droplets are formed and moved using an electrowetting technique. A binary mixing apparatus and method are also provided. The apparatus comprises an array of electrodes, an electronic controller communicating with the electrodes, a sample droplet supply area communicating with the array, and an additive droplet supply area communicating with the array. The electronic controller alternately energizes and de-energizes selected electrodes to carry out droplet-to-droplet binary mixing operations to obtain one or more droplets having a target mixing ratio.
    Type: Grant
    Filed: September 24, 2002
    Date of Patent: February 12, 2008
    Assignee: Duke University
    Inventors: Vamsee K. Pamula, Michael G. Pollack, Philip Y. Paik, Hong Ren, Richard B. Fair
  • Patent number: 7329535
    Abstract: Provided are an apparatus for circulating a carrier fluid having two or more chambers or sections, an apparatus for amplifying a nucleic acid using the same, and a chip containing the same. The apparatus for circulating a carrier fluid includes two or more chambers maintained at different temperatures, each chamber having an inlet valve containing a pneumatic air pressure port for controlling inflow of the carrier fluid to the chamber (inlet pneumatic air pressure port), and an outlet valve containing a pneumatic air pressure for controlling outflow of the carrier fluid from the chamber (outlet pneumatic air pressure port), wherein the chambers are sequentially connected such that the outlet valve of one chamber is connected to the inlet valve of an adjacent chamber in a direction the fluid flows.
    Type: Grant
    Filed: November 11, 2002
    Date of Patent: February 12, 2008
    Assignee: Samsung Electronics Co., Ltd.
    Inventors: Kwang-wook Oh, Geun-bae Lim, Young-sun Lee, Yoon-kyoung Cho
  • Patent number: 7329391
    Abstract: Microfluidic devices for manipulating relatively dense materials, such as colloidal rod particles, are provided. Microfluidic devices for separating a denser first material from a less-dense second material are provided. Methods of manipulating a relatively dense first material, for example, colloidal rod particles, and separating the first material from a less-dense second material, are provided. Methods of marking samples or sample components with relatively dense materials, are also provided.
    Type: Grant
    Filed: December 8, 2003
    Date of Patent: February 12, 2008
    Assignee: Applera Corporation
    Inventor: David Cox
  • Patent number: 7323143
    Abstract: The present invention provides, in certain embodiments, improved microfluidic systems and methods for fabricating improved microfluidic systems, which contain one or more levels of microfluidic channels. The inventive methods can provide a convenient route to topologically complex and improved microfluidic systems. The microfluidic systems provided according to the invention can include three-dimensionally arrayed networks of fluid flow paths therein including channels that cross over or under other channels of the network without physical intersection at the points of cross over. The microfluidic networks of the invention can be fabricated via replica molding processes, also provided by the invention, utilizing mold masters including surfaces having topological features formed by photolithography.
    Type: Grant
    Filed: November 25, 2002
    Date of Patent: January 29, 2008
    Assignee: President and Fellows of Harvard College
    Inventors: Janelle R. Anderson, Daniel T. Chiu, Rebecca J. Jackman, Oksana Cherniavskaya, Justin Cooper McDonald, George M. Whitesides
  • Patent number: 7323140
    Abstract: The present invention relates to a system and method for moving samples, such as fluid, within a microfluidic system using a plurality of gas actuators for applying pressure at different locations within the microfluidic. The system includes a substrate which forms a fluid network through which fluid flows, and a plurality of gas actuators integral with the substrate. One such gas actuator is coupled to the network at a first location for providing gas pressure to move a microfluidic sample within the network. Another gas actuator is coupled to the network at a second location for providing gas pressure to further move at least a portion of the microfluidic sample within the network. A valve is coupled to the microfluidic network so that, when the valve is closed, it substantially isolates the second gas actuator from the first gas actuator.
    Type: Grant
    Filed: February 15, 2002
    Date of Patent: January 29, 2008
    Assignee: HandyLab, Inc.
    Inventors: Kalyan Handique, Gene Parunak
  • Patent number: 7320775
    Abstract: There is provided a flow cell assembly in which a shuttle supports and positions a capillary with its end extending beyond the shuttle. The flow cell assembly facilitates the replacement of a flow cell which is damaged or with flow cells having capillaries of different size or shape.
    Type: Grant
    Filed: May 14, 2002
    Date of Patent: January 22, 2008
    Assignee: Guava Technologies, Inc.
    Inventors: Thomas E. Kochy, Roger A. O'Neill, Terah W. Smiley, Vidal O. Smith
  • Patent number: 7318912
    Abstract: Microfluidic devices capable of combining discrete fluid volumes generally include channels for supplying different fluids toward a sample chamber and means for establishing fluid communication between the fluids within the chamber. Discrete fluid plugs are defined from larger fluid volumes before being combined. Certain embodiments utilize adjacent chambers or subchambers divided by a rupture region such as a frangible seal. Further embodiments utilize one or more deformable membranes and/or porous regions to direct fluid flow. Certain devices may be pneumatically or magnetically actuated.
    Type: Grant
    Filed: May 16, 2002
    Date of Patent: January 15, 2008
    Assignee: Nanostream, Inc.
    Inventors: Marci Pezzuto, Eugene Dantsker
  • Patent number: 7314567
    Abstract: In the method for mixing-free transport of homogeneous liquids in microchannels a heterogeneous liquid flow (14) is autonomously divided into portions, transported over a distance and then autonomously recombined. When the flow is divided into portions, individual volumes of a second liquid (16), which may be but must not be homogeneous, are introduced between volume fractions lying one behind the other of the heterogeneous liquid flow (14). While the volume fractions of the heterogeneous liquid (14) that lye close to one another would mix with each other without this measure being taken, separation of the individual volume fractions allows these volume fractions, and thus the heterogeneous liquid (14), to be transported over large distances without any mixing occurring.
    Type: Grant
    Filed: May 26, 2003
    Date of Patent: January 1, 2008
    Assignee: ProtoLife Srl.
    Inventors: Patrick Wagler, John Simpson McCaskill, Tobias Foster
  • Patent number: 7312085
    Abstract: The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or detection of particles, such as cells and/or beads. The invention provides systems, including apparatus, methods, and kits, for the microfluidic manipulation and/or analysis of particles, such as cells, viruses, organelles, beads, and/or vesicles. The invention also provides microfluidic mechanisms for carrying out these manipulations and analyses. These mechanisms may enable controlled input, movement/positioning, retention/localization, treatment, measurement, release, and/or output of particles. Furthermore, these mechanisms may be combined in any suitable order and/or employed for any suitable number of times within a system.
    Type: Grant
    Filed: April 1, 2003
    Date of Patent: December 25, 2007
    Assignee: Fluidigm Corporation
    Inventors: Hou-Pu Chou, Antoine Daridon, Kevin Farrell, Brian Fowler, Yish-Hann Liau, Ian D. Manger, Hany Ramez Nassef, William Throndset