Xanthan; I.e., Xanthomonas-type Heteropolysaccharides Patents (Class 435/104)
  • Patent number: 5736137
    Abstract: Disclosed herein are therapeutic treatment protocols designed for the treatment of B cell lymphoma. These protocols are based upon therapeutic strategies which include the use of administration of immunologically active mouse/human chimeric anti-CD20 antibodies, radiolabeled anti-CD20 antibodies, and cooperative strategies comprising the use of chimeric anti-CD20 antibodies and radiolabeled anti-CD20 antibodies.
    Type: Grant
    Filed: November 3, 1993
    Date of Patent: April 7, 1998
    Assignee: Idec Pharmaceuticals Corporation
    Inventors: Darrell R. Anderson, Nabil Hanna, John E. Leonard, Roland A. Newman, Mitchell E. Reff, William H. Rastetter
  • Patent number: 5705368
    Abstract: The process comprises heat-treating a xanthan gum fermented broth, and consecutively treating the broth first with alkaline protease and then with lysozyme or in reverse order, and thereafter recovering xanthan gum from the treated broth. A clear aqueous solution of xanthan gum may be obtained without complex procedures.
    Type: Grant
    Filed: December 15, 1992
    Date of Patent: January 6, 1998
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Kanji Murofushi, Taira Homma, Shigehiro Nagura, Richard Armentrout
  • Patent number: 5702927
    Abstract: A xanthan gum having an improved transmittance is disclosed. Further, the transmittance of the xanthan gum is at least 75% in an 1% by weight aqueous solution. The xanthan gum is prepared by subjecting a strain ATCC 55429 or ATCC 55298 to submerged fermentation conditions to produce a broth containing the gum and debris. The broth is then subjected to heating at a temperature from 45.degree. to 70 .degree. C. for a period of time of at least one half to two hours and at a pH of 9 to 12.5. Two enzyme treatments are performed using an alkaline protease and lysozyme of which the order of their application is irrelevant to the recovery of the xanthan gum. The enzymes are applied at specific temperatures and time periods as well as pH conditions such as a protease is applied at a temperature of 40.degree. to 65 .degree. C. for a period of from 20 minutes to five hours at a pH range of 6 to 10; and the lysozyme is applied at a temperature of 25.degree. to 60 .degree. C.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: December 30, 1997
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Kanji Murofushi, Taira Homma, Shigehiro Nagura, Richard W. Armentrout
  • Patent number: 5679556
    Abstract: A xanthan gum-containing fermented solution is subjected to an enzyme treatment for solubilizing the microbial cells present in the fermented solution. While the fermented solution having undergone the enzyme treatment is maintained at a temperature of 50.degree. C. to 80.degree. C., and xanthan gum is precipitated by adding an hydrophilic organic solvent incapable of dissolving xanthan gum to the fermented solution. When a rotary turbine is used, the precipitate can be cut with a shearing cutter to recover a finely-divided fibrous product.
    Type: Grant
    Filed: June 7, 1995
    Date of Patent: October 21, 1997
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Taira Homma, Kanji Murofushi, Shigehiro Nagura
  • Patent number: 5610037
    Abstract: Polysaccharide biopolymers, e.g., xanthan gum, are produced by the aerobic microbial fermentation of a carbohydrate in an aqueous nutrient medium containing starch as a source of carbon, and wherein the fermentation is carried out in the presence of a saccharide-specific amylolytic enzyme.
    Type: Grant
    Filed: January 27, 1995
    Date of Patent: March 11, 1997
    Assignee: Rhone-Poulenc Chimie
    Inventor: Patrick Cros
  • Patent number: 5595892
    Abstract: The process includes heat-treating a xanthan gum fermented broth, and consecutively treating the broth first with alkaline protease and then with lysozyme or in reverse order, and thereafter recovering xanthan gum from the treated broth. A clear aqueous solution of xanthan gum may be obtained Without complex procedures.
    Type: Grant
    Filed: November 28, 1994
    Date of Patent: January 21, 1997
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Kanji Murofushi, Taira Homma, Shigehiro Nagura, Richard W. Armentrout
  • Patent number: 5580763
    Abstract: A method for the fermentation production of xanthan gum which comprises the step of carrying out culture by using a water-soluble inorganic nitrogen component alone as the nitrogen source of a production medium, and by mixing and using the water-soluble inorganic nitrogen component and a water-insoluble organic nitrogen component as the nitrogen sources of a seed fermentation medium.
    Type: Grant
    Filed: August 25, 1994
    Date of Patent: December 3, 1996
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Taira Honma, Shigehiro Nagura, Kanji Murofushi
  • Patent number: 5559015
    Abstract: Methods for the recombinant-DNA mediated production of xanthan gum and gum variants structurally related to xanthan are disclosed. The methods in part involve the synthesis of these polysaccharides in anaerobic and/or denitrifying hosts.In particular, plasmids pX209 and pRK290-H366 are disclosed which contain the genes, isolated from X. campestris, encoding Transferase I, Transferases II, Transferase III, Transferase IV, Transferase V, Ketalase, Acetylase and Polymerase. These plasmids have been deposited in the American Type Culture Collection under Accession Nos. 67051 and 67049, respectively.
    Type: Grant
    Filed: December 2, 1994
    Date of Patent: September 24, 1996
    Assignee: Getty Scientific Development Company
    Inventors: Michael A. Capage, Daniel H. Doherty, Michael Betlach, Rebecca W. Vanderslice
  • Patent number: 5536651
    Abstract: The present invention relates toa mutant strain of Xanthomonas campestris;a method of preparing xanthan by fermentation of said strain; anda non-viscous xanthan capable of being obtained by said method.
    Type: Grant
    Filed: March 15, 1995
    Date of Patent: July 16, 1996
    Assignees: Sanofi, Societe Nationale Elf Aguitaine
    Inventor: Marc Salome
  • Patent number: 5514791
    Abstract: Variant xanthan gums are provided which include a water-soluble polysaccharide polymer comprising repeating pentamer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:2:1, and a water-soluble polysaccharide polymer comprising repeating tetramer units having a D-glucose:D-mannose:D-glucuronic acid ratio of about 2:1:1. The D-glucose moieties are linked in a beta- 1,4! configuration. The inner D-mannose moieties are linked in an alpha- 1,3! configuration, generally to alternate glucose moieties. The D-glucuronic acid moieties are linked in a beta- 1,2! configuration to the inner mannose moieties. The outer mannose moieties are linked to the glucuronic acid moieties in a beta- 1,4! configuration. Processes for preparing the polysaccharide polymers are also provided.
    Type: Grant
    Filed: April 25, 1994
    Date of Patent: May 7, 1996
    Assignee: Getty Scientific Development Company
    Inventors: Daniel H. Doherty, Donna M. Ferber, John D. Marrelli, Rebecca W. Vanderslice, Randal A. Hassler
  • Patent number: 5480785
    Abstract: A process of producing xanthan gum using a Xanthomonas microorganism is provided. Increased yields of xanthan gum are obtained by using mannose as part of the carbohydrate component of the fermentation feedstock. Preferably, the carbohydrate component of the feedstock is a mixture of mannose and glucose. More preferable is a mixture comprising 5 to 60% by weight mannose, particularly 20 to 45% by weight mannose, the balance being glucose although minor amounts of other sugars, e.g. maltose, may also be present. The carbohydrate component of the feedstock may be in the form of a raffinate stream from a glucose epimerization.
    Type: Grant
    Filed: January 21, 1994
    Date of Patent: January 2, 1996
    Assignee: Cerestar Holding B.V.
    Inventors: Jean-Claude M. G. de Troostembergh, Roland H. F. Beck, Benedicte L. T. De Wannemaeker
  • Patent number: 5472870
    Abstract: A method of increasing xanthan gum production, comprising culturing a Xanthomonas campestris strain having a xanthan-increasing modification in a culture medium, wherein the modification is selected from the group consisting of (1) a mutation causing rifampicin-resistance; (2) a mutation causing bacitracin-resistance; or (3) exogenous genetic information controlling the synthesis of xanthan; and separating xanthan from the culture medium, is provided along with specific DNA sequences and Xanthomonas campestris strains showing increased xanthan gum production. The strain is preferably ATCC 55429.
    Type: Grant
    Filed: January 27, 1992
    Date of Patent: December 5, 1995
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Thomas J. Pollock, Linda Thorne
  • Patent number: 5451515
    Abstract: The present invention relates to the discovery of a new bacteria of the genus Alteromonas which has been found to attract oyster larvae by the production of compounds involved in melanin synthesis. More specifically, the present invention contemplates a method for inducing the settlement and metamorphosis of Crassostrea virginica larvae by induction with certain metabolic substances produced by the present bacteria and its altered variants. Furthermore, the present invention is directed to other and derivative metabolic products which can be employed for their desired utility and application. In accordance with the present invention, it has been discovered that ammonia (NH.sub.3) initiates settlement and metamorphosis of the oyster larvae in the same manner as the natural phenomena.
    Type: Grant
    Filed: April 15, 1994
    Date of Patent: September 19, 1995
    Assignee: Research Corporation Technologies, Inc.
    Inventors: Ronald M. Weiner, Rita R. Colwell, Dale B. Bonar, Steven L. Coon, Marianne Walsh
  • Patent number: 5446014
    Abstract: Precipitate-free aqueous compositions, well adapted as agricultural chemicals, e.g., crop protectants, are comprised of at least one water soluble cationic organic compound, e.g., a pesticidally active quaternary ammonium compound, and a thickening amount of a xanthan gum having an intrinsic viscosity of less than 3,500 cm.sup.3 /g.
    Type: Grant
    Filed: July 26, 1991
    Date of Patent: August 29, 1995
    Assignee: Rhone-Poulenc Chimie
    Inventors: Jean-Luc Schuppiser, Magali Knipper
  • Patent number: 5434078
    Abstract: The present invention relates to the unexpected discovery that xanthan gum can be produced from filtered whey or milk products, including whey permeates and milk permeates, by a fermentation process which uses organisms which are capable of converting lactose to xanthan gum.
    Type: Grant
    Filed: May 11, 1993
    Date of Patent: July 18, 1995
    Assignees: Shin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.
    Inventors: Thomas J. Pollock, Richard W. Armentrout
  • Patent number: 5401644
    Abstract: The present invention relates toa mutant strain of Xanthomonas campestris;a method of preparing xanthan by fermentation of said strain; anda non-viscous xanthan capable of being obtained by said method.
    Type: Grant
    Filed: December 27, 1991
    Date of Patent: March 28, 1995
    Assignees: Sanofi, Societe Nationale Elf Aquitaine
    Inventor: Marc Salome
  • Patent number: 5401659
    Abstract: An isolated endogly canase having activity against polysaccharides having the subunit backbone structure: ##STR1## wherein Glc is glucose, GlcA is glucuronic acid, Rha is rhamnose, Man is mannose, X may be Rha or Man, and wherein the reducing end of the polymer is toward the X residue of the backbone is disclosed. A method for producing the compound by culturing Bacillus ATCC 55294, the method for selecting Bacillus ATCC 55294 and method for using the endoglycanase are also disclosed.
    Type: Grant
    Filed: May 31, 1994
    Date of Patent: March 28, 1995
    Assignees: Shin-Etsu Bio, Inc., Shin-Etsu Chemical Co., Ltd.
    Inventors: Linda P. Thorne, Richard W. Armentrout, Thomas J. Pollock, Marcia J. Mikolajczak
  • Patent number: 5354671
    Abstract: A novel enzyme composition capable of lysing or digesting cells or cellular debris of polysaccharide-producing bacteria in the presence of the exopolysaccharides without any significant degradation of the exopolysaccharides. Methods for preparing the enzyme composition, using the composition to clarify mixtures of polysaccharides and cellular debris, and pure cultures for making the enzyme are disclosed.
    Type: Grant
    Filed: June 26, 1992
    Date of Patent: October 11, 1994
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventor: Thomas J. Pollock
  • Patent number: 5348675
    Abstract: The novel heteropolysaccharide BM07, useful, e.g., as a thickening/stabilizing/suspending agent for a wide variety of industrial applications, is produced by fermenting a nutrient medium containing at least one source of assimilable carbon with a strain of Agrobacterium tumefaciens I-736, or recombinant or mutant thereof.
    Type: Grant
    Filed: January 19, 1993
    Date of Patent: September 20, 1994
    Assignee: Rhone-Poulenc Chimie
    Inventors: Gabriel Ullmann, Alain Jarry
  • Patent number: 5340743
    Abstract: A method of increasing xanthan gum production, comprising culturing a Xanthomonas campestris strain having a xanthan-increasing modification in a culture medium, wherein the modification is selected from the group consisting of (1) a mutation causing rifampicin-resistance; (2) a mutation causing bacitracin-resistance; or (3) exogenous genetic information controlling the synthesis of xanthan; and separating xanthan from the culture medium, is provided along with specific DNA sequences and Xanthomonas campestris strains showing increased xanthan gum production.
    Type: Grant
    Filed: February 13, 1992
    Date of Patent: August 23, 1994
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Thomas J. Pollock, Linda Thorne
  • Patent number: 5328689
    Abstract: The present invention relates to the discovery of a new bacteria of the genus Alteromonas which has been found to attract oyster larvae by the production of compounds involved in melanin synthesis. More specifically, the present invention contemplates a method for inducing the settlement and metamorphosis of Crassostrea virginica larvae by induction with certain metabolic substances produced by the present bacteria and its altered variants. Furthermore, the present invention is directed to other and derivative metabolic products which can be employed for their desired utility and application. In accordance with the present invention, it has been discovered that ammonia (NH.sub.3) initiates settlement and metamorphosis of the oyster larvae in the same manner as the natural phenomena.
    Type: Grant
    Filed: March 8, 1993
    Date of Patent: July 12, 1994
    Assignee: Research Corporation Technologies, Inc.
    Inventors: Ronald M. Weiner, Rita R. Colwell, Dale B. Bonar, Steven L. Coon, Marianne Walsh
  • Patent number: 5310677
    Abstract: A method of increasing xanthan gum production, comprising culturing a Xanthomonas campestris strain having a xanthan-increasing modification in a culture medium, wherein the modification is selected from the group consisting of (1) a mutation causing rifampicin-resistance; (2) a mutation causing bacitracin-resistance; or (3) exogenous genetic information controlling the synthesis of xanthan; and separating xanthan from the culture medium, is provided along with specific DNA sequences and Xanthomonas campestris strains showing increased xanthan gum production.
    Type: Grant
    Filed: November 5, 1992
    Date of Patent: May 10, 1994
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Thomas J. Pollock, Linda Thorne
  • Patent number: 5300429
    Abstract: P. elodea mutants are produced which produce gellan gum broth which contains no detectable amount of poly-.beta.-hydroxy-butyrate (PHB).
    Type: Grant
    Filed: February 22, 1993
    Date of Patent: April 5, 1994
    Assignee: Merck & Co., Inc.
    Inventors: John K. Baird, Joseph M. Cleary
  • Patent number: 5273892
    Abstract: An acid heteropolysaccharide P-318 which comprises galactose, galacturonic acid, N-acetylfucosamine and pyruvic acid in a ratio of about 2:3:1:1, a sulfated heteropolysaccharide P-318 having a 10% or less sulfur content which is prepared by sulfating said acid heteropolysaccharide and a process for preparing an acid heteropolysaccharide P-318 which comprises culturing Pseudomonas sp. 318 to produce the acid heteropolysaccharide P-318 and collecting the thus produced acid heteropolysaccharide P-318. The sulfated polysaccharide P-318 of the invention shows a strong antiviral activity in spite of a lower content of sulfate.
    Type: Grant
    Filed: January 21, 1992
    Date of Patent: December 28, 1993
    Assignee: Teikoku Seiyaku Kabushiki Kaisha
    Inventor: Koichi Okutani
  • Patent number: 5198469
    Abstract: Disclosed are a bath lotion and a body lotion both consisting essentially of a fermented liquid of high polymer complex polysaccharides. The fermented liquid is obtained by activating and heat-treating a xanthane gum with microorganisms for carbohydrate fermentation. The bath lotion and body lotion are effective and advantageous to health and have a pharmaceutical effect.
    Type: Grant
    Filed: June 10, 1991
    Date of Patent: March 30, 1993
    Inventor: Shigenobu Sakata
  • Patent number: 5194386
    Abstract: A method of increasing xanthan gum production, comprising culturing a Xanthomonas campestris strain having a xanthan-increasing modification in a culture medium, wherein the modification is selected from the group consisting of (1) a mutation causing rifampicin-resistance; (2) a mutation causing bacitracin-resistance; or (3) exogenous genetic information controlling the synthesis of xanthan; and separating xanthan from the culture medium, is provided along with specific DNA sequences and Xanthomonas campestris strains showing increased xanthan gum production.
    Type: Grant
    Filed: February 13, 1990
    Date of Patent: March 16, 1993
    Assignees: Shin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
    Inventors: Thomas J. Pollock, Linda Thorne
  • Patent number: 5153320
    Abstract: Heteropolysaccharide 105-4, prepared by fermentation of a new unnamed Pseudomonas species ATCC 53923 is useful as an industrial thickening, suspending or stabilizing agent.
    Type: Grant
    Filed: July 25, 1989
    Date of Patent: October 6, 1992
    Assignee: Pfizer Inc.
    Inventor: Bruce L. Dasinger
  • Patent number: 5091376
    Abstract: Two new bacterial strains designated Zoogloea ramigera 115SL and Zoogloea ramigera 115SLR, a rifampicin resistant derivative of 115SL, have been developed. These strains are derived from the wild type Zoogloea ramigera 115, ATCC 25935. The two new strains produce a novel exopolysaccharide (EPS) and have several desirable characteristics that are absent from the parent strain, including improved culture properties, since they do not produce an EPS capsule layer like that of the parent 115 strain. The 115SL EPS is instead excreted as a slime layer which is not confined to the immediate area surrounding the cells. Since cells are not trapped within a floc where they grow at a reduced rate or die because of nutrient starvation, the new strains have more consistent and reproducible growth cycles and increased growth rates. As a consequence, exopolysaccharide production is more consistent and titers are higher. The separation of the EPS from the cells is also much easier and more economical.
    Type: Grant
    Filed: May 17, 1990
    Date of Patent: February 25, 1992
    Assignee: Massachusetts Institute of Technology
    Inventors: Donald D. Easson, Jr., Oliver P. Peoples, Anthony J. Sinskey
  • Patent number: 5017479
    Abstract: Process for obtaining a mass of polysaccharide-producing microorganisms, consisting in conducting the growth of the microorganisms in a medium containing an enzyme which hydrolyzes the formed polysaccharide.Application in a process for the production of polysaccharide in two stages, in which the growth stage takes place in the presence of an enzyme, particularly for the production of scleroglucane using sclerotium type fungi.
    Type: Grant
    Filed: February 17, 1989
    Date of Patent: May 21, 1991
    Assignee: Sanofi
    Inventor: Bruno Eyssautier
  • Patent number: 5017237
    Abstract: A process for facilitating the removal of undesired contamination from a surface, comprising the steps:(a) applying a solution containing a polysaccharide and a solvent therefor onto said surface before it is subjected to contamination, said polysaccharide being capable of forming a redissolvable or swelling solid film upon drying;(b) allowing the applied solution to dry to form a solid film on said surface;(c) treating the coated surface with a liquid capable of redissolving the film or providing for swelling thereof; and(d) removing the undesired contamination by removing the dissolved or swelled surface layer of the film.
    Type: Grant
    Filed: January 16, 1990
    Date of Patent: May 21, 1991
    Assignee: Bioboat AB
    Inventor: Sigfrid Svensson
  • Patent number: 5013654
    Abstract: A novel strain of Pseudomonas aeruginosa, designated as strain SB-1, is capable of growth on hydrocarbon substrates having 10 to 32 or more carbon atoms, during which growth there is secreted into the culture medium an emulsifier which can be recovered and used for such applications as reducing the viscosity of crude oil in secondary recovery methods, as well as in oil spill management and the cleaning of oil-contaminated vessels and pipelines. A novel mutant strain of P. aeruginosa SB-1, designated SB-3, has the property of growing on solid (C.sub.20 +) paraffins but not on liquid alkanes. The selective degradation by strain SB-3 of the solid paraffinic components in crude oil is advantageous in reducing the viscosity of the oil for improving the recovery thereof from oil wells. A novel revertant strain of P.
    Type: Grant
    Filed: March 5, 1990
    Date of Patent: May 7, 1991
    Assignee: The Board of Trustees of the University of Illinois
    Inventors: Santimoy Banerjee, Jeffrey S. Karns, Ananda M. Chakrabarty
  • Patent number: 5010186
    Abstract: Novel deacetylated polysaccharide biopolymers having improved viscosity/filtrability/injectability, well adopted for the secondary and tertiary recovery of oil deposits, are conveniently prepared by (i) acidifying an aqueous polysaccharide composition, e.g., a carbohydrate fermentation broth, with nitric acid to a pH of from about 2 to 0.1, (ii) heat-treating said acidified composition at a temperature of from about 50.degree. to 100.degree. C. for from about 5 to 60 minutes, and (iii) cooling said heat-treated composition and adjusting the pH thereof to a value of from about 5 to 7.
    Type: Grant
    Filed: September 13, 1989
    Date of Patent: April 23, 1991
    Assignee: Rhone-Poulenc Specialties Chimiques
    Inventors: Patrick Cros, Robert Pipon
  • Patent number: 4999047
    Abstract: Pseudoplastic aqueous concentrates, well adapted for dilution with water to constitute precipitate-free sprayable compositions useful, e.g., for plant protection, are formulated in water from a thickening amount of a xanthan gum having a content in pyruvic acid moieties of less than about 2%, and at least one water soluble organic cationic compound, e.g., a cationic pesticide.
    Type: Grant
    Filed: July 18, 1989
    Date of Patent: March 12, 1991
    Assignee: Rhone-Poulenc Chimie
    Inventor: Jean-Luc Schuppiser
  • Patent number: 4996153
    Abstract: A novel mixed bacterial culture isolated from soil by enrichment culture techniques produces a xanthanase enzyme complex which is stable to 65.degree. C. in the presence of salt. These properties render the heat-stable, salt-tolerant zanthanase useful for in situ degradation of xanthan gum in petroleum recovery fluids and other thickened industrial brines.
    Type: Grant
    Filed: August 29, 1989
    Date of Patent: February 26, 1991
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventors: Martin C. Cadmus, Morey E. Slodki
  • Patent number: 4940663
    Abstract: The invention relates to a process for the fermentation of carbohydrates by bacteria of the genus Xanthomonas for the production of a polysaccharide of the xanthane type, in which the source of nitrogen consists of a gelatin with a molecular weight of less than 5000.Application: preparation of xanthane.
    Type: Grant
    Filed: June 22, 1988
    Date of Patent: July 10, 1990
    Assignee: SANOFI, Societe Anonyme
    Inventor: Bruno Eyssautier
  • Patent number: 4923803
    Abstract: The galactitol level in the body fluid, e.g. blood or urine, is determined by the method that comprises allowing a body fluid sample to contact in vitro with a bacterium capable of producing D-tagatose from galactitol, and measuring the D-tagatose. Such bacterium is of the genus Arthrobacter, specifically, Arthrobacter globiformis ST-48 FERM P-7592 or its mutant, or of the genus Pseudomonas. The method is useful for detecting galactose dysbolism.
    Type: Grant
    Filed: August 8, 1988
    Date of Patent: May 8, 1990
    Assignee: Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
    Inventors: Ken Izumori, Shuzo Sakai
  • Patent number: 4911909
    Abstract: Monoclonal antibodies to angiotensin II and the continuous hybrid monoclonal cell lines for their production are provided. These antibodies are useful in the diagnosis and treatment of angiotensin II-induced hypertension.
    Type: Grant
    Filed: January 2, 1987
    Date of Patent: March 27, 1990
    Assignee: E. I. Du Pont De Nemours and Company
    Inventor: Thomas M. Reilly
  • Patent number: 4898819
    Abstract: Aqueous saline solutions of ordered heteropolysaccharides useful, e.g., as displacement fluids in the secondary or tertiary recovery of crude oil from subterranean formations thereof, are stabilized against viscosity loss over temperature and time without adversely affecting the filterability thereof, by purging dissolved oxygen therefrom by degassing same with carbon dioxide, and then treating the degassed solutions with an effective amount of a reducng/deoxidizing agent as to maintain the pH thereof at a value ranging from 5 to 7.
    Type: Grant
    Filed: December 5, 1985
    Date of Patent: February 6, 1990
    Assignee: Rhone-Poulenc Specialities Chimiques
    Inventor: Jean-Louis Linossier
  • Patent number: 4894335
    Abstract: Stable oil-in-water emulsions are prepared containing a relatively high concentration of from 8 to 60% by weight of a heteropolysaccharide bipolymer, e.g., xanthan gum preferably, the emulsions contain greater than 15 up to 60% biopolymer, 40 to 99 parts of an aqueous phase containing 10 to 60% by weight biopolymer and 40% to 90% by weight water, 1 to 60 parts oil and 1 to 40% with respect to the aqueous phase and oil of a surface active agent. The emulsions are prepared by combining powdered biopolymer with water, oil and surfactant or by concentrating an oil-in-water emulsion containing the biopolymer such as by ultrafiltration. A fermentation wort containing the biopolymer may be used in preparing the emulsion.
    Type: Grant
    Filed: March 16, 1987
    Date of Patent: January 16, 1990
    Assignee: Rhone-Poulenc Specialites Chimiques
    Inventors: Michel Peignier, Michel Garcia, Alain Senechal, Pierre Gagne, Dominique Lespinasse, Jean-Bernard Chaux
  • Patent number: 4891318
    Abstract: For the fermentative preparation of polysaccharides, in particular xanthane, water, production medium (sugars, nutrient salts), and an inoculation material of a suitable aerobic bacterium are mixed in a reactor as reaction components, the production medium being exposed to fermentation while an oxygen-containing gas is being supplied. The object is to avoid the usual low yield of such a process and to reject an expensive and sensitive alternative construction (circulation tube). According to the invention, the reaction components are introduced into a reactor vessel in a circulating flow by means of pumping means, which circulating flow comprises a rising flow and a descending flow which are separated from each other by one or more partitions disposed in the reactor vessel. The mixing is brought about because the rising flow and/or the descending flow is passed essentially in the form of a plug flow through one or more static in-line mixers.
    Type: Grant
    Filed: June 8, 1987
    Date of Patent: January 2, 1990
    Assignee: Cooperatieve Vereniging Suiker Unie U.S.
    Inventors: Nicolaas M. G. Oosterhuis, Kees Koerts
  • Patent number: 4886746
    Abstract: A novel mixed bacterial culture isolated from soil by enrichment culture techniques produces a xanthanase enzyme complex which is stable to 65.degree. C. in the presence of salt. These properties render the heat-stable, salt-tolerant xanthanase useful for in situ degradation of xanthan gum in petroleum recovery fluids and other thickened industrial brines.
    Type: Grant
    Filed: May 10, 1988
    Date of Patent: December 12, 1989
    Assignee: The United States of America as represented by the Secretary of Agriculture
    Inventors: Martin C. Cadmus, Morey E. Slodki
  • Patent number: 4874044
    Abstract: A process for recovering hydrocarbons from a subterranean hydrocarbon bearing formation penetrated by an injection well and a production well which comprises injecting an aqueous drive fluid into the formation and forcing the drive fluid through the formation to recover the hydrocarbons from the production well.
    Type: Grant
    Filed: October 11, 1988
    Date of Patent: October 17, 1989
    Assignee: Texaco Inc.
    Inventors: Peter D. Robison, Arthur J. Stipanovic
  • Patent number: 4871665
    Abstract: Process for the preparation of exocellular biopolymers having a thickening effect in aqueous media by aerobic culture of microorganism strains forming the biopolymers in an aqueous nutrient medium present as disperse aqueous phase in a water/oil emulsion (W/O-emulsion) stable under fermentation conditions using W/O emulsifiers for emulsion formation and stabilization, in which the oil phase is used in quantities below 50% by volume, based on the mixture as a whole, and fatty acid dialkanolamides are used as the W/O emulsifiers.
    Type: Grant
    Filed: December 19, 1986
    Date of Patent: October 3, 1989
    Assignee: Henkel Kommanditgesellschaft auf Aktien
    Inventor: Holger Viehweg
  • Patent number: 4868293
    Abstract: A polysaccharide polymer is disclosed which is a better viscosifier of water than xanthan gum. The polysaccharide polymer and its non-acetylated form, are comprised of glucose and mannose moieties in a ratio of about 2:1. The invention also discloses Xanthomonas mutants which produce the polysaccharide polymer but which do not produce xanthan gum. Methods of preparing the polysaccharide polymers and of their use are also described.
    Type: Grant
    Filed: September 22, 1987
    Date of Patent: September 19, 1989
    Assignee: Getty Scientific Development Company
    Inventors: Rebecca W. Vanderslice, Patrick Shannon
  • Patent number: 4851393
    Abstract: A method for utilizing a purified exocellular polysaccharide, Zooglan, produced by Zoogloea ramigera, as a viscosity modifier, gellant, drag reducing agent, dispersant, flocculant, or emulsifying agent, wherein the properties of the polymer are dependent on, and can therefore be controlled by varying, the pH, the concentration of polymer, the ionic strength of the solution, and the processing parameters such as mechanical shear.The overall conformation of Zooglan, which is responsible for its functional properties, can be manipulated with pH and/or ionic strength of the solution. For example, the intrinsic viscosity of Zooglan (estimated m.w.=2.times.10.sup.7) can vary from 16 to 170 dl/g in aqueous solution as a function of pH between 2-9 and salt concentration between 0 and 1M KCl. Zooglan is a polyelectrolyte above pH 2. The Zooglan chain is relatively stiff, with a stiffness parameter of 0.02. Zooglan solutions at a concentration above 0.
    Type: Grant
    Filed: July 28, 1986
    Date of Patent: July 25, 1989
    Assignee: Massachusetts Institute of Technology
    Inventors: ChoKyun Rha, Pasawadee Pradipasena, TetsuHisa Nakamura, Donald D. Easson Jr., Anthony J. Sinskey
  • Patent number: 4797473
    Abstract: A hybridoma is provided which yields a monoclonal antibody which binds to an epitope on an unreduced, nonenzymatically-glycated plasma protein, and which is substantially free of cross-reactivity with the corresponding non-glycated plasma protein.
    Type: Grant
    Filed: June 25, 1986
    Date of Patent: January 10, 1989
    Assignee: Regents of the University of Minnesota
    Inventors: Joseph F. Tarsio, Leo T. Furcht
  • Patent number: 4792415
    Abstract: Derivatized anionic gums and compositions thereof are disclosed. The gum derivatives are prepared by reacting non-derivatized gum with quaternary ammonium compounds in an amount equal to or greater than the stoichiometric amount required for complete derivatization. The derived gums are soluble in organic solvents.
    Type: Grant
    Filed: April 13, 1987
    Date of Patent: December 20, 1988
    Assignee: Merck & Co., Inc.
    Inventor: George T. Colegrove
  • Patent number: 4775632
    Abstract: Aqueous solutions of polysaccharide biopolymers, e.g., Xanthomonas/carbohydrate fermentation worts, are treated with mutanase-containing enzymes to improve the filterability and injectability thereof, and are well adapted, e.g., for secondary and tertiary hydrocarbon (petroleum) recovery by waterflooding therewith.
    Type: Grant
    Filed: August 30, 1984
    Date of Patent: October 4, 1988
    Assignee: Rhone-Poulenc Specialites Chimiques
    Inventors: Jean-Pierre Gozard, Alain Jarry, Alain Luccioni
  • Patent number: 4758356
    Abstract: Process for preparing Xanthomonas heteropolysaccharide from Xanthomonas campestris NCIB 11854 and use of the latter, e.g. as viscosity modifier in an aqueous solution, and in a drilling fluid and use in connection with well treatments, and enhanced oil recovery.
    Type: Grant
    Filed: March 20, 1987
    Date of Patent: July 19, 1988
    Assignee: Shell Oil Company
    Inventor: John D. Downs
  • Patent number: 4752580
    Abstract: Process for preparing Xanthomonas heteropolysaccharide from Xanthomonas campestris NCIB 11854 and use of the latter, e.g. as viscosity modifier in an aqueous solution, and in a drilling fluid and use in connection with well treatments, and enhanced oil recovery.
    Type: Grant
    Filed: June 29, 1984
    Date of Patent: June 21, 1988
    Assignee: Shell Oil Company
    Inventor: John D. Downs