Nitrogen As Only Ring Hetero Atom Patents (Class 435/121)
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Publication number: 20140199734Abstract: The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c], from the corresponding ketones of general formula [1][a], by using novel transaminases. These novel transaminases are selected from two different groups: either from a group of some 20 proteins with sequences as specified herein, or from a group of proteins having transaminase activity and isolated from a microorganism selected from the group of organisms consisting of Rahnella aquatilis, Ochrobactrum anthropi, Ochrobactrum tritici, Sinorhizobium morelense, Curtobacterium pusilllum, Paecilomyces lilacinus, Microbacterium ginsengisoli, Microbacterium trichothecenolyticum, Pseudomonas citronellolis, Yersinia kristensenii, Achromobacter spanius, Achromobacter insolitus, Mycobacterium fortuitum, Mycobacterium frederiksbergense, Mycobacterium sacrum, Mycobacterium fluoranthenivorans, Burkholderia sp., Burkholderia tropica, Cosmospora episphaeria, and Fusarium oxysporum.Type: ApplicationFiled: July 14, 2011Publication date: July 17, 2014Applicant: DSM IP ASSETS B.V.Inventors: Martin Schürmann, Wijnand Peter Helena Peeters, Natascha Hubertina Smeets, Helmut Schwab, Kerstin Steiner, Kateryna Mykoiayivna Lypetska, Gernot Strohmeier
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Publication number: 20140199735Abstract: The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.Type: ApplicationFiled: April 12, 2012Publication date: July 17, 2014Applicant: CODEXIS, INC.Inventors: Anupam Gohel, Derek Smith, Brian Wong, Joly Sukumaran, Wan Lin Yeo, Steven J. Collier, Scott Novick
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Patent number: 8778654Abstract: Recombinant bacteria for producing deoxyviolacein and uses thereof are provided, wherein the recombinant bacteria is obtained by introducing the deoxyviolacein synthesis-related gene cluster into Escherichia coli BL21-CodonPlus (DE3)-RIL or Pseudomonas putida mt-2. The deoxyviolacein synthesis-related gene cluster is obtained by knocking out VioD gene from the violacein synthesis-related gene cluster composed of VioA, VioB, VioC, VioD and VioE, and the nucleotide sequence is as shown in the SEQ ID NO: 1 in the sequence listing. A method for producing deoxyviolacein by fermenting the recombinant bacteria to produce deoxyviolacein by using L-tryptophan as substrate is provided. The method has high efficiency of deoxyviolacein production, the deoxyviolacein produced is convenient to be extracted, and simple to be separated and purified.Type: GrantFiled: April 22, 2009Date of Patent: July 15, 2014Assignee: Tsinghua UniversityInventors: Xinhui Xing, Peixia Jiang
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Patent number: 8778642Abstract: Aspects of the invention relate to methods for the production of difunctional alkanes in host cells. In particular, aspects of the invention describe components of genes associated with the difunctional alkane production from carbohydrate feedstocks in host cells. More specifically, aspects of the invention describe metabolic pathways for the production of adipic acid, aminocaproic acid, caprolactam, and hexamethylenediamine via 2-ketopimelic acid.Type: GrantFiled: June 4, 2012Date of Patent: July 15, 2014Assignee: Celexion, LLCInventors: Brian M. Baynes, John Michael Geremia
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Patent number: 8771997Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.Type: GrantFiled: October 12, 2011Date of Patent: July 8, 2014Assignee: Ajinomoto Co., Inc.Inventors: Yasuaki Takakura, Hiroomi Ogino, Masakazu Sugiyama, Kenichi Mori, Eri Tabuchi, Koki Ishikawa, Uno Tagami, Hidemi Fujii
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Publication number: 20140186902Abstract: This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, 6-hydroxyhexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.Type: ApplicationFiled: December 13, 2013Publication date: July 3, 2014Applicant: INVISTA North America S. a r.l.Inventors: Adriana Leonora Botes, Alex Van Eck Conradie
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Publication number: 20140178460Abstract: This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.Type: ApplicationFiled: August 30, 2013Publication date: June 26, 2014Applicant: VERENIUM CORPORATIONInventors: David P. WEINER, Alexander VARVAK, Toby RICHARDSON, Mircea PODAR, Ellen BURKE, Shaun HEALEY
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Publication number: 20140162326Abstract: Methods for forming ammonium salts of C4 diacids in a fermentation process with removal of divalent metal carbonate salts are disclosed. The pH of fermentation broths for production of C4 diacids is controlled by adding alkaline oxygen containing calcium or magnesium compounds, which forms divalent metal salts of the diacids. The divalent metal salts of the diacids are substituted with ammonium by introduction of ammonium salts at elevated temperature and pressure forming soluble ammonium salts thereof. C02 or bicarbonate is simultaneously added to the fennentation media at the elevated temperature and pressure. Reducing the temperature and pressure forms insoluble divalent metal carbonate salts that are separated from the solubilized ammonium diacid salts.Type: ApplicationFiled: July 9, 2012Publication date: June 12, 2014Inventors: ChiCheng Ma, Todd Werpy
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Patent number: 8728775Abstract: The present invention relates to a method for preparing 2-pyrrolidone using biomass, comprising: a step (a) of culturing a microorganism which contains glutamate decarboxylase as a whole-cell catalyst in a culture medium containing glutamic acid or glutamate so as to prepare 4-amino butyric acid; a step (b) of filtering the 4-amino butyric acid from the culture medium in order to obtain the 4-amino butyric acid; and a step (c) of converting the 4-amino butyric acid into 2-pyrrolidone. The present invention provides a series of processes for preparing 2-pyrrolidone from glutamic acid or glutamate using biomass. According to the present invention, 4-amino butyric acid is prepared using a microorganism as a whole cell, and preferably, 4-amino butyric acid which has not undergone a complicated refining process such as a crystallization process is directly used to prepare 2-pyrrolidone at a high yield rate in an economically advantageous manner.Type: GrantFiled: September 30, 2011Date of Patent: May 20, 2014Assignee: Daesang CorporationInventors: Dong Cheol Park, Ki Kueon Kang, Hyun Jung Park, Seo Hyoung Kim, Jae Young Oh
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Publication number: 20140134672Abstract: Bioreactors, and particularly, photobioreactors having a reactor chamber and surge driver, and methods for using these devices, for example, for the production of carbon-based products are provided. The reactor chamber provides a housing for microorganisms and culture medium. The surge driver produces a surge of the microorganisms and/or culture medium in the reactor chamber.Type: ApplicationFiled: July 5, 2012Publication date: May 15, 2014Inventors: Max B. Tuttman, David A. St. Angelo
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Publication number: 20140127764Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.Type: ApplicationFiled: January 8, 2014Publication date: May 8, 2014Applicant: BASF SEInventors: Hartwig Schröder, Stefan Haefner, Gregory Von Abendroth, Rajan Hollmann, Aline Raddatz, Hansgeorg Ernst, Hans Gurski
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Publication number: 20140113338Abstract: The invention relates to an alpha-ketopimelic acid decarboxylase enzyme that is a homologue of SEQ ID NO:2, comprising at least one mutation selected from a group of substitutions listed in the specification, to a method for preparing 5-formyl valeric acid (hereinafter also referred to as ‘5-FVA’), to a method for preparing 6-aminocaproic acid (hereinafter also referred to as ‘6-ACA’), to a method for preparing ?-caprolactam (hereinafter referred to as ‘caprolactam’) from 6-ACA, to a method for the preparation of adipic acid, to a method for preparing diaminohexane. The invention further relates to a host cell which may be used in a method according to the invention and to a polynucleotide encoding an alpha-ketopimelic acid decarboxylase enzyme.Type: ApplicationFiled: March 30, 2012Publication date: April 24, 2014Applicant: DSM IP ASSETS B.V.Inventors: Axel Christoph Trefzer, Stefanus Cornelis Hendrikus Josef Turk, Jan Metske Van Der Laan, Iise De Lange, Denise Ilse Jacobs
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Patent number: 8685673Abstract: The present invention provides a method for in vitro producing an indole derivative in a one-pot reaction. The method for producing a rhamnosylated indolocarbazole compound includes the steps of transforming a plasmid carrying a gene encoding N-glycosyltransferase into a bacterial strain; expressing the gene encoding N-glycosyltransferase in the bacterial strain; lysing the bacterial strain to obtain a crude enzyme extract; and adding TDP-glucose, an indolocarbazole aglycone and a metal ion in the crude enzyme extract for performing an enzymatic reaction to form the rhamnosylated indolocarbazole compound. Alternatively, the method for producing an indole-3-carboxaldehyde analog includes the steps of transforming a plasmid carrying a gene encoding NokA of Nocardiopsis sp.Type: GrantFiled: August 2, 2010Date of Patent: April 1, 2014Assignee: National Chiao Tung UniversityInventor: Hsien-Tai Chiu
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Patent number: 8673598Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.Type: GrantFiled: February 12, 2010Date of Patent: March 18, 2014Assignee: BASF SEInventors: Hartwig Schroder, Stefan Haefner, Gregory Von Abendroth, Rajan Hollmann, Aline Raddatz, Hansgeorg Ernst, Hans Gurski
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Publication number: 20140073018Abstract: Disclosed is a method for the preparation of carbamic acid (R)-1-aryl-2-tetrazolyl-ethyl esters, comprising the enantioselective enzyme reduction of a 1-aryl-2-tetrazolyl-ethyl ketone to form a (R)-1-aryl-2-tetrazolyl-ethyl alcohol and the carbamation of said alcohol.Type: ApplicationFiled: February 26, 2013Publication date: March 13, 2014Applicant: SK BIOPHARMACEUTICALS CO. LTD.Inventors: Sang Chul Lim, Moo Yong Uhm, Dae Won Lee, Hui Ho Kim, Dong Ho Lee, Hyun Seok Lee
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Publication number: 20140024088Abstract: Process for the enzymatic synthesis of the compound of formula (I), (7S)-1-(3,4-dimethoxy-bicyclo[4.2.0]octa-1,3,5-trien-7-yl)N-methyl methanamine: and application in the synthesis of ivabradine and addition salts thereof with a pharmaceutically acceptable acid.Type: ApplicationFiled: July 15, 2013Publication date: January 23, 2014Inventors: Sandrine PEDRAGOSA-MOREAU, François LEFOULON, Francisco MORIS VARAS, Javier GONZALEZ SABIN
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Patent number: 8633001Abstract: A process for producing optically active succinimide derivatives as key intermediates of (3R)-2?-(4-bromo-2-fluorobenzyl)spiro{pyrrolidine-3,4?(1?H)-pyrrolo[1,2-a]pyrazine}-1?,2,3?,5(2?H)-tetraone, which comprises the following reaction steps.Type: GrantFiled: February 5, 2010Date of Patent: January 21, 2014Assignees: Dainippon Sumitomo Pharma Co., Ltd., Kyowa Hakko Bio Co., Ltd.Inventors: Masaji Kasai, Shinji Kita, Tadashi Ogawa, Hideaki Tokai
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Publication number: 20140004575Abstract: Provided is a method for efficiently producing from a ketone compound an optically active amino compound useful as an intermediate of a drug, an agricultural chemical, or the like. Provided are: a polypeptide having aminotransferase activity that is increased in stereoselectivity, heat resistance, and resistance to amine compounds compared to the wild type enzyme by means of modifying an aminotransferase derived from Pseudomonas fluorescens; a gene encoding the polypeptide; and a transformant that expresses the gene at a high level.Type: ApplicationFiled: March 9, 2012Publication date: January 2, 2014Applicant: Kaneka CorporationInventors: Noriyuki Ito, Shigeru Kawano, Yoshihiko Yasohara
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Patent number: 8613857Abstract: A product is enriched by a method for the processing of a reaction solution, in which: a) a pH value of the reaction solution is adjusted to less than 4; wherein said reaction solution comprises a whole-cell catalyst, an aqueous component, and an organic component, wherein the organic component contains a product to be enriched; and b) the reaction solution is filtered in the presence of a filter aid, thereby enriching the product.Type: GrantFiled: June 21, 2007Date of Patent: December 24, 2013Assignee: Evonik Degussa GmbHInventors: Harald Groeger, Claudia Rollmann, Helge Werner, Francoise-Christine Chamouleau, Dittmar Orzewski
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Publication number: 20130337485Abstract: The present invention discloses a process for preparing an active pharmaceutical ingredient (API) or intermediates thereof, notably particular step in the synthesis of an intermediate useful for example in the preparation of statins, by using an enzyme capable of catalyzing oxidation or dehydrogenation. The invention further provides an expression system effectively translating said enzyme. In addition, the invention relates to a specific use of such enzyme for preparing API or intermediate thereof, and in particular for preparing statin or intermediate thereof.Type: ApplicationFiled: December 20, 2011Publication date: December 19, 2013Applicant: LEK PHARMACEUTICALS D.DInventors: Peter Mrak, Tadeja Zohar, Matej Oslaj, Gregor Kopitar
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Publication number: 20130310570Abstract: Methods that include latent 1,3-dipole-functional compounds are disclosed herein. The latent 1,3-dipole-functional compound (e.g., an oxime) can be used to form an active 1,3-dipole-functional compound (e.g., a nitrile oxide) that can be used to react with a cyclic alkyne in a dipolar cycloaddition reaction.Type: ApplicationFiled: September 27, 2011Publication date: November 21, 2013Applicant: UNIVERSITY OF GEORIGA RESEARCH FOUNDATION, INC.Inventors: Geert-Jan Boons, Frederic Friscourt, Petr A. Ledin, Ngalle Eric Mbua
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Publication number: 20130302859Abstract: The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds.Type: ApplicationFiled: May 9, 2013Publication date: November 14, 2013Applicant: CODEXIS,INC.Inventors: CHEN Haibin, Steven J. Collier, Jovana NAZOR, Joly SUKUMARAN, Derek SMITH, Jeffrey C. MOORE, Gregory HUGHES, Jacob JANEY, Gjalt HUISMAN, Scott NOVICK, Nicholas AGARD, Oscar ALVIZO, Gregory COPE, Wan Lin YEO, Stephanie NG
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Patent number: 8574875Abstract: The present invention relates to a novel bacterial strain designated DD1, which has the ability to produce organic acids, in particular succinic acid (SA), which was originally isolated from bovine rumen, and is capable of utilizing glycerol as a carbon source; and variant strains derived there from retaining said capability; as well as to methods of producing organic acids, in particular succinic acid by making use of said microorganism.Type: GrantFiled: August 14, 2008Date of Patent: November 5, 2013Assignee: BASF SEInventors: Edzard Scholten, Dirk Dägele, Stephan Haefner, Hartwig Schröder
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Publication number: 20130280766Abstract: The present invention provides for a polyketide synthase (PKS) capable of synthesizing an even-chain or odd-chain diacid or lactam or diamine. The present invention also provides for a host cell comprising the PKS and when cultured produces the even-chain diacid, odd-chain diacid, or KAPA. The present invention also provides for a host cell comprising the PKS capable of synthesizing a pimelic acid or KAPA, and when cultured produces biotin.Type: ApplicationFiled: October 31, 2011Publication date: October 24, 2013Applicant: The Regents of the Unversity of CaliforniaInventors: Jeffrey L. Fortman, Andrew Hagen, Leonard Katz, Jay D. Keasling, Sean Poust, Jingwei Zhang, Sergey Zotchev
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Patent number: 8563278Abstract: A process for producing efficiently glutamic acid derivatives (including salts thereof) such as monatin is described. This process entails converting a substituted ?-keto acid of formula (1) into a glutamic acid derivative of formula (2) in the presence of an enzyme catalyzing conversion of the same.Type: GrantFiled: August 10, 2010Date of Patent: October 22, 2013Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Nao Funakoshi, Yusuke Amino, Shigeru Kawahara, Tadashi Takemoto
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Publication number: 20130273616Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: ApplicationFiled: April 12, 2013Publication date: October 17, 2013Inventors: Paula M. HICKS, Sara C. MCFARLAN
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Publication number: 20130273617Abstract: The present invention provides a method for in vitro producing an indole derivative in a one-pot reaction. The method for producing a rhamnosylated indolocarbazole compound includes the steps of transforming a plasmid carrying a gene encoding N-glycosyltransferase into a bacterial strain; expressing the gene encoding N-glycosyltransferase in the bacterial strain; lysing the bacterial strain to obtain a crude enzyme extract; and adding TDP-glucose, an indolocarbazole aglycone and a metal ion in the crude enzyme extract for performing an enzymatic reaction to form the rhamnosylated indolocarbazole compound. Alternatively, the method for producing an indole-3-carboxaldehyde analog includes the steps of transforming a plasmid carrying a gene encoding NokA of Nocardiopsis sp.Type: ApplicationFiled: June 18, 2013Publication date: October 17, 2013Inventor: Hsien-Tai Chiu
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Publication number: 20130252288Abstract: The invention relates to a process for the preparation of a chiral compound, comprising enantioselectively reducing a carbon-carbon double bond of an ?,?-unsaturated compound in a mixture comprising both an E isomer and a Z isomer of the ?,?-unsaturated compound, wherein both E isomer and Z isomer are converted in the presence of a hydrogenation catalyst.Type: ApplicationFiled: July 11, 2008Publication date: September 26, 2013Inventors: Stefaan Marie André De Wildeman, Henricus Martinus Maria Straatman, Gerardus Karel Maria Verzijl, Linda M. Vermote, Andreas Hendrikus Maria De Vries
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Patent number: 8535920Abstract: The present invention relates to a process for producing efficiently glutamic acid derivatives (including salts thereof) such as monatin by converting a substituted ?-keto acid of formula (1) into a glutamic acid derivative of formula (2) in the presence of an enzyme catalyzing conversion of the same.Type: GrantFiled: November 20, 2006Date of Patent: September 17, 2013Assignee: Ajinomoto Co., Inc.Inventors: Masakazu Sugiyama, Kunihiko Watanabe, Nao Funakoshi, Yusuke Amino, Shigeru Kawahara, Tadashi Takemoto
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Publication number: 20130224807Abstract: The invention relates to methods for enriching monomer content in a cycloalkane oxidation process mixed organic waste stream. In particular, the methods involve combining a biocatalyst with a mixed organic waste stream from a cycloalkane oxidation process, and enzymatically converting dimeric and/or oligomeric components of said waste stream into monomeric components. The methods may enrich the content of diacids, adipic acid, and/or other ?,?-difunctional C6 alkanes in the mixed organic waste stream. Additionally, the treated mixed organic waste streams may have improved burning efficiency.Type: ApplicationFiled: June 15, 2012Publication date: August 29, 2013Applicant: INVISTA North America S.a r.l.Inventors: Paul S. Pearlman, Changlin Chen, Adriana L. Botes
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Publication number: 20130210091Abstract: Process for the enzymatic synthesis of the compound of formula (I): wherein R1 represents a hydrogen atom or an alkyl group. Application in the synthesis of ivabradine and addition salts thereof with a pharmaceutically acceptable acid.Type: ApplicationFiled: February 5, 2013Publication date: August 15, 2013Applicant: LES LABORATOIRES SERVIERInventor: LES LABORATOIRES SERVIER
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Publication number: 20130210090Abstract: Embodiments of the invention relate to the enzymatic conversion of bioderived feedstocks to commercially valuable chemicals. The enzymatic conversions of the embodiments of the invention offer the potential for lower cost routes to these value-added chemicals. Some of the chemicals that are useful include nylon intermediates such as caprolactam, adipic acid, 1,6-hexamethylene diamine; butanediols such as 1,4-butanediol, 1,3-butanediol, and 2,3-butanediol; butanols such as 1-butanol, and 2-butanol; succinic acid, butadiene, isoprene, and 3-hydroxypropanoic acid.Type: ApplicationFiled: June 15, 2012Publication date: August 15, 2013Applicant: INVISTA North America S.a.r.l.Inventors: Paul S. PEARLMAN, Changlin CHEN, Adriana L. BOTES
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Publication number: 20130210092Abstract: A method for efficiently producing an optically active amino compound useful as an intermediate for pharmaceutical preparations, agricultural chemicals, or the like, from a ketone compound is provided. Specifically, a polypeptide having high resistance to a water-soluble organic solvent and novel transaminase activity for generating (S)-1-benzyl-3-pyrrolidinone with high optical purity of 93% or more, a gene encoding the same, and a transformant expressing the gene at a high level are also provided herein.Type: ApplicationFiled: September 28, 2011Publication date: August 15, 2013Applicant: Kaneka CorporationInventors: Toru Nagasawa, Toyokazu Yoshida, Kouichi Ishida, Noriyuki Ito, Shigeru Kawano, Yoshihiko Yasohara
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Publication number: 20130203131Abstract: The present invention relates to a method for preparing 2-pyrrolidone using biomass, comprising: a step (a) of culturing a microorganism which contains glutamate decarboxylase as a whole-cell catalyst in a culture medium containing glutamic acid or glutamate so as to prepare 4-amino butyric acid; a step (b) of filtering the 4-amino butyric acid from the culture medium in order to obtain the 4-amino butyric acid; and a step (c) of converting the 4-amino butyric acid into 2-pyrrolidone. The present invention provides a series of processes for preparing 2-pyrrolidone from glutamic acid or glutamate using biomass. According to the present invention, 4-amino butyric acid is prepared using a microorganism as a whole cell, and preferably, 4-amino butyric acid which has not undergone a complicated refining process such as a crystallization process is directly used to prepare 2-pyrrolidone at a high yield rate in an economically advantageous manner.Type: ApplicationFiled: September 30, 2011Publication date: August 8, 2013Applicant: Daesang CorporationInventors: Dong Cheol Park, Ki Kueon King, Hyun Jung Park, Seo Hyoung Kim, Jae Young Oh
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Patent number: 8501436Abstract: Disclosed is a method for the preparation of carbamic acid (R)-1-aryl-2-tetrazolyl-ethyl ester, comprising the asymmetric reduction of arylketone and the carbamation of alcohol.Type: GrantFiled: October 14, 2009Date of Patent: August 6, 2013Assignee: SK Biopharmaceuticals Co. Ltd.Inventors: Sang Chul Lim, Moo Yong Uhm, Nahm Ryune Cho, Dae Won Lee, Ju Young Lee, Hui Ho Kim, Dong Ho Lee, Hyun Seok Lee, Se Il Lee
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Publication number: 20130196013Abstract: Compositions and methods for controlling molluscs, members of the Gastropoda and Bivalvia classes which includes but is not limited to lactones, lactams, carbamates, amides, and/or carboxylic acid containing compounds as active ingredients and/or compounds derived from Pseudomonas and/or Erwinia. Also provided are methods and compositions for increasing the efficacy of chemical and biological control for invasive molluscs in open waters, power plants, and drinking water treatment facilities under coldwater conditions.Type: ApplicationFiled: March 15, 2013Publication date: August 1, 2013Applicant: MARRONE BIO INNOVATIONS, INC.Inventor: MARRONE BIO INNOVATIONS, INC.
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Publication number: 20130196388Abstract: A process for efficiently producing optically active succinimide derivatives as key intermediates of (3R)-2?-(4-bromo-2-fluorobenzyl)spiro{pyrrolidine-3,4?(1?H)-pyrrolo[1,2-a]pyrazine}-1?,2,3?,5(2?H)-tetraone, which comprises the following reaction steps, and the step 2 is performed by using a non-animal-derived enzyme.Type: ApplicationFiled: July 28, 2011Publication date: August 1, 2013Applicants: KYOWA HAKKO BIO CO., LTD., DAINIPPON SUMITOMO PHARMA CO., LTD.Inventors: Shinji Kita, Kazuya Tsuzaki, Eitora Yamamura
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Publication number: 20130183728Abstract: This document describes biochemical pathways for producing adipic acid, caprolactam, 6-aminohexanoic acid, hexamethylenediamine or 1,6-hexanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C6 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on CoA-dependent elongation enzymes or analogues enzymes associated with the carbon storage pathways from polyhydroxyalkanoate accumulating bacteria.Type: ApplicationFiled: December 14, 2012Publication date: July 18, 2013Applicant: INVISTA NORTH AMERICA S.A R.L.Inventor: INVISTA North America S.a.r.l.
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Publication number: 20130149751Abstract: The present invention relates to a process for preparing aminocyclohexyl ether compounds of Formula I: or the pharmaceutically acceptable salts and esters thereof. In particular, the instant invention is directed towards a process for preparing (1R,2R)-2-[(3R)-Hydroxypyrrolidinyl]-1-(3,4-dimethoxyphenethoxy)-cyclohexane as well as various intermediates.Type: ApplicationFiled: August 8, 2011Publication date: June 13, 2013Inventors: John Limanto, Gregory L. Beutner, Jingjun Yin, Artis Klapars, Eric R. Ashley, Hallena R. Strotman, Matthew D. Truppo, Cheol K. Chung, Gregory Hughes, Zhijian Liu, Brendan Grau, Jacob Janey
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Publication number: 20130131306Abstract: Processes for producing caprolactam (CL) and derivatives thereof from adipic acid (AA) obtained from fermentation broths containing diammonium adipate (DAA) or monoammonium adipate (MAA).Type: ApplicationFiled: June 10, 2011Publication date: May 23, 2013Applicant: BIOAMBER S.A.S.Inventors: Olan S. Fruchey, Leo E. Manzer, Dilum Dunuwila, Brian T. Keen, Brooke A. Albin, Nye A. Clinton, Bernard D. Dombek
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Publication number: 20130130333Abstract: The invention provides novel processes for making ethyl-4-cyano-3-hydroxybutyrate, e.g., (R)-ethyl 4-cyano-3-hydroxybutyric acid, and 4-cyano-3-hydroxybutyric acid. The invention provides protocols for making and 4-cyano-3-hydroxybutyric acid and ethyl-4-cyano-3-hydroxybutyrate by whole cell processes, cell lysate processes, “one pot processes” and “multi-pot” processes using a variety of parameters.Type: ApplicationFiled: January 25, 2013Publication date: May 23, 2013Applicant: VERENIUM CORPORATIONInventor: Verenium Corporation
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Publication number: 20130121978Abstract: Compositions and methods for controlling molluscs, members of the Gastropoda and Bivalvia classes which includes but is not limited to lactones, lactams, carbamates, amides, and/or carboxylic acid containing compounds as active ingredients and/or compounds derived from Pseudomonas and/or Erwinia. Also provided are methods and compositions for increasing the efficacy of chemical and biological control for invasive molluscs in open waters, power plants, and drinking water treatment facilities under coldwater conditions.Type: ApplicationFiled: December 17, 2012Publication date: May 16, 2013Applicant: MARRONE BIO INNOVATIONS, INC.Inventor: Marrone Bio Innovations, Inc.
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Patent number: 8440434Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: GrantFiled: May 20, 2008Date of Patent: May 14, 2013Assignee: Cargill, IncorporatedInventors: Paula M. Hicks, Sara C. McFarlan
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Publication number: 20130116398Abstract: Processes for making caprolactam (CL) from monoammonium adipate (MAA) and/or adipic aicd (AA) obtained from a clarified diammonium adipate-containing (DAA-containing) fermentation broth or MAA-containing fermentation broth and converting the MAA or AA to the CL with hydrogen in the presence of a catalyst at selected temperatures and pressures.Type: ApplicationFiled: June 10, 2011Publication date: May 9, 2013Applicant: BIOAMBER S.A.S.Inventors: Olan S. Fruchey, Leo E. Manzer, Dilum Dunuwila, Brian T. Keen, Brooke A. Albin, Nye A. Clinton, Bernard D. Dombek
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Patent number: 8435765Abstract: Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.Type: GrantFiled: May 20, 2008Date of Patent: May 7, 2013Assignee: Cargill, IncorporatedInventors: Timothy W. Abraham, Douglas C. Cameron, Paula M. Hicks, Sara C. McFarlan, James R. Millis, John Rosazza, David P. Weiner, Lishan Zhao
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Publication number: 20130101571Abstract: Methods and compositions for detoxifying nitrobenzodiazepines with nitroreductase mutants.Type: ApplicationFiled: March 23, 2012Publication date: April 25, 2013Applicant: Academia SinicaInventors: Andrew H-J. Wang, Shiuan-Woei Lin Wu, Fu-Chuo Peng, Che-An Wu
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Publication number: 20130084608Abstract: The present invention provides fungal proteases and improved fungal strains that are deficient in protease production.Type: ApplicationFiled: August 29, 2012Publication date: April 4, 2013Applicant: Codexis, Inc.Inventors: Lorand Szabo, Zsolt Molnar, Attila Laszlo Nemeth
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Publication number: 20130084610Abstract: It is an object of the present invention to provide a procedure for realizing inexpensive and simple production of 3-indole-pyruvic acid. A transformant is made using a polynucleotide having a specific nucleotide sequence encoding a protein having an oxidase activity, and oxidase is generated by culturing the transformant in a medium to accumulate the oxidase in the medium and/or the transformant. Further, tryptophan is converted into 3-indole-pyruvic acid in the presence of the transformant and/or a culture thereof to produce 3-indole-pyruvic acid.Type: ApplicationFiled: November 26, 2012Publication date: April 4, 2013Inventors: Yasuaki TAKAURA, Seiichi Hara, Toshiki Taba, Shunichi Suzuki, Masakazu Sugiyama, Kunihiko Watanabe, Kenzo Yokozeki
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Publication number: 20130066066Abstract: The presently disclosed subject matter demonstrates that ThnG and ThnQ enzymes encoded by the thienamycin gene cluster in Streptomyces cattleya oxidize the C-2 and C-6 moieties of carbapenems, respectively. ThnQ stereospecifically hydroxylates PS-5 giving N-acetyl thienamycin. ThnG catalyzes sequential desaturation and sulfoxidation of PS-5, giving PS-7 and its sulfoxide. The ThnG and ThnQ enzymes are relatively substrate selective, but give rise to the oxidative diversity of carbapenems produced by S. cattleya, and orthologues likely function similarly in allied streptomyces.Type: ApplicationFiled: December 13, 2010Publication date: March 14, 2013Applicant: THE JOHNS HOPKINS UNIVERSITYInventors: Craig Arthur Townsend, Micah Jeffrey Bodner, Ryan Martin Phelan, Michael Francis Freeman
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Publication number: 20130052699Abstract: The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.Type: ApplicationFiled: May 4, 2011Publication date: February 28, 2013Applicant: CODEXIS, INC.Inventors: Michael Crowe, Oscar Alvizo, Behnaz Behrouzian, Yong Koy Bong, Steven J. Collier, Anupam Gohel, Jagadeesh Mavinahalli, Naga Modukuru, Emily Mundorff, Derek Smith, Shiwei Song, Wan Lin Yeo